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ARHGAP20 (NM_001258416) Human Tagged ORF Clone
CAT＃: RG235201
- TrueORF®
ARHGAP20 (tGFP-tagged) - Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 3
ORF Plasmid: DDK
"NM_001258416" in other vectors (2)
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CNY 12,730.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RARHOGAP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235201 representing NM_001258416
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATTTTGGATTATAATAAATAAGAAAATGAAAACACTAGCAGAAAGGAGGAGGAGCGCTCCATCTC TTATCCTGGATAAAGCCCTACAAAAACGGCCTACTACCAGGGACAGTCCTTCTGCTAGTGTTGACACATG CACATTTCTGTCATCATTAGTGTGCTCCAATAGGACTCTGCTGATTGATGGCCGGGCAGAACTCAAAAGA GGCCTCCAGAGGCAGGAGCGGCATCTTTTCCTATTCAATGATCTGTTTGTTGTGGCCAAAATCAAATATA ACAATAACTTTAAGATAAAAAATAAAATTAAATTAACTGATATGTGGACAGCAAGCTGTGTGGATGAAGT GGGAGAAGGCAACACCAATGCCATGAAATCCTTTGTTTTGGGCTGGCCCACAGTGAACTTTGTGGCCACT TTCAGTTCTCCAGAACAAAAGGACAAATGGCTCTCTCTCCTTCAGAGATACATCAATCTAGAGAAAGAAA AGGACTACCCGAAGAGCATTCCCCTCAAAATCTTCGCCAAGGACATTGGGAATTGTGCCTACTCTAAAAC TATAACAGTAATGAATTCAGATACAGCGAATGAAGTTATCAACATGTCATTACCAATGCTAGGGATAACT GGCTCTGAGAGAGATTACCAGTTGTGGGTCAATTCTGGCAAAGAAGAGGCTCCATACCCACTCATTGGGC ATGAATATCCATATGGAATTAAAATGAGCCATCTTCGAGACTCTGCACTCCTGACACCGGGATCAAAGGA CTCTACCACCCCTTTCAACCTCCAGGAGCCCTTCCTTATGGAACAGCTCCCCCGAGAGATGCAGTGCCAG TTCATCCTGAAGCCCAGCCGCCTGGCTGCAGCCCAGCAACTGAGTGATTCAGGTCATAAGACATTTAAAA GGAGAAGATCTATCATAAACTGGGCCTTCTGGCGAGGTTCTAGCACTCACCTGGACAACTTGCCCTCATC GCCAACATCACCTATGCCAGGACAGCTCTTTGGAATTTCTCTGCCAAATATTTGTGAGAATGACAATCTG CCCAAACCTGTCTTGGATATGCTTTTCTTTCTTAATCAAAAAGGACCTCTCACCAAAGGTATCTTCAGGC AATCAGCCAATGTGAAATCCTGCAGAGAACTAAAAGAGAAATTGAATTCTGGAGTCGAAGTACACCTAGA CTGTGAATCTATTTTTGTGATAGCATCTGTCTTAAAGGATTTTCTGCGAAATATTCCAGGAAGTATTTTT TCATCAGATCTCTATGATCACTGGGTCTCTGTAATGGATCAAGGAAATGATGAAGAGAAAATAAATACTG TTCAAAGGCTATTAGACCAGCTTCCGAGAGCCAATGTTGTTCTCCTAAGGTATCTTTTTGGGGTGTTACA CAACATTGAGCAACATTCCTCATCCAATCAGATGACTGCATTTAATTTAGCTGTGTGTGTCGCTCCAAGT ATTCTTTGGCCTCCTGCTTCCTCCAGCCCAGAACTAGAAAACGAATTTACAAAAAAGGTTTCCCTGCTTA TACAATTTCTGATTGAGAATTGCCTTAGGATATTTGGAGAAGAAATCACTTCCCTCTTCAGAGAGGTTTC AGTGAGATGTGACACTAGAGAGAATGCCTCAGATATTTCTTGCTTTCAACTGAATGACTCCTCCTATGAC AGCTTGGAAAATGAGCTAAATGAGGATGTTGATGCACCATGCAGTGACTTGGTAAAGAAACTTGGCCAGG GGAGCAGAAGCATGGACTCTGTCTTAACCCTCAGTGACTATGATCTTGACCAGCCCGAGGTGGAAGGCCT TTTAACCCTAAGCGACTTTGACTTGGCCCATTCTAAAGATGAAGATGTTCAAATGAAACGGCCTCTTGAA TCCAAGCCGGTGAACATTTTAGTGTACACAAAGATCCCACTGCGGGATCATGCCAGGGCCCCATCTGCCA TGTGCACACCCAGCTACCTGTCCACAGCTGCAGCAAATGCTGCAAAAAGCCTGAGGCGACACCGGCGTTG CTCAGAGCCCAGCATCGACTATCTGGATTCAAAGCTTTCCTACCTCAGGGAGTTTTATCAGAAAAAGCTA CGCAAGTCCAGCTGTGATGCAATTCTTTCTCAAAAAGATGAAGACTATCTGAAGCAGAATCAACCCCTCC AGGAGGAAGGAAAGACATGTTTTAAACAGAGTTTAGTCACAGGCACTGATGTCAGCAAGAAAAATGCCAC TACTCAAAACACTAAGAAGAAAAGCTTGTCTGGTAGTGAAGGAAATCACGTGAAACTTTTCCCTAAGTCT AAGCCAGTGGCCATTTCTGTGGCATCTTATAGTCCTATGTCCTCACAGGATCATTCCAAGAACCAGCCCT TTGATGTGAATACATCTGGATACTCCCCACCACACACAGCAGATGCCCTCAAGGGTCCAAGGACACATCG GCGCTGCTCAGAGCCCAACATAGAAGACCAGAACCGCAAGCTGACCTATCTCAGGGGAATTTATTCAAAG AAACAACATAAAACCAGCTGTGAAGCTGGTCTCTTGCATGGAGAGGAGGATTATCTCAAACGGCATAAGT CTTTGCAAATGGAGGGGCAGAAGCTCATTAATCAGAGTTTAGTCATGGGGATTGAGGTGGGCAAGAGTAG TGCCACAAACCAAAACACTGAGAAGGTTTTACCCCCAAGATTAAACCTTTGCCCAAGGACCAGCTATTCC AGCTTATCCTCCCCAGGCACTTCCCCATCCGGCTCATCAGTAAGCTCCCAAGACAGTGCTTTTTCTCAGA TTTCTGAACACTCTGTGTTTACACCCACTGAGACTTCCTCTCCAATAGATTGCACTTTTCAGGCTCAGAG AAAACGGGAAGACCTTTCTCCTGACTTTAGCAATGCCAGCCATGTTTCCGGAATGCCCGGTCCCTCATCA GGGCAGGCTTGCAGCCGCCCAGCCTATACAAAGAAGGACACCATGGAGTGGCATTCACAAATGCATTCTG TAACTCTTCATCCCAGCACATGGTTGAGAAATGGTGTGGCCAGTTTGAAAAACTGGTCCCTCAAAAAGAA AGCAAAGGCAGCCAGACCAGAGGAAGAGAAAATAGCTTCTCCAAAAGGACCCTTAGAGCCACCCCCACAT GCTTCTGGTGTTCCAGAAGCCAACTCACTGCAAGAGGAACAAAAAGACTTGCCCTTAAGGGCAGCTGAAG GACTGTCCCCTGTGCAGTCAGCCCAAAGGTGTAGTTCTTCTCCCTTCCAGGACTCAGAGAGACACTGTAG CTCTCCATTCAGCCTGGTGGAGAGCAGACTTAAGCTGTGCATGAAGTCACATGAGGAAATAGAGCCTGGT AGTCAGAGCTCTTCTGGTTCTCTGCCTTGGGAAAGAGCCTCAGCCAGCTCTTGGACTCTAGAGGATGCGA CCAGCCCAGACTCAGGGCCTACAGTGGTCTGCGACATTGAGGACAGGTATTTAACCAAAGACATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235201 representing NM_001258416
Red=Cloning site Green=Tags(s) MTFWIIINKKMKTLAERRRSAPSLILDKALQKRPTTRDSPSASVDTCTFLSSLVCSNRTLLIDGRAELKR GLQRQERHLFLFNDLFVVAKIKYNNNFKIKNKIKLTDMWTASCVDEVGEGNTNAMKSFVLGWPTVNFVAT FSSPEQKDKWLSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVMNSDTANEVINMSLPMLGIT GSERDYQLWVNSGKEEAPYPLIGHEYPYGIKMSHLRDSALLTPGSKDSTTPFNLQEPFLMEQLPREMQCQ FILKPSRLAAAQQLSDSGHKTFKRRRSIINWAFWRGSSTHLDNLPSSPTSPMPGQLFGISLPNICENDNL PKPVLDMLFFLNQKGPLTKGIFRQSANVKSCRELKEKLNSGVEVHLDCESIFVIASVLKDFLRNIPGSIF SSDLYDHWVSVMDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCVAPS ILWPPASSSPELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDSSYD SLENELNEDVDAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKRPLE SKPVNILVYTKIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQKKL RKSSCDAILSQKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLFPKS KPVAISVASYSPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGIYSK KQHKTSCEAGLLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRTSYS SLSSPGTSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPGPSS GQACSRPAYTKKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEPPPH ASGVPEANSLQEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEIEPG SQSSSGSLPWERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001258416 |
| ORF Size | 3495 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001258416.1, NP_001245345.1 |
| RefSeq Size | 6085 bp |
| RefSeq ORF | 3498 bp |
| Locus ID | 57569 |
| UniProt ID | Q9P2F6 |
| Gene Summary | The protein encoded by this gene is an activator of RHO-type GTPases, transducing a signal from RAP1 to RHO and impacting neurite outgrowth. [provided by RefSeq, Sep 2016] |
Documents
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| SDS |
Resources
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