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Flightless I (FLII) (NM_001256264) Human Tagged ORF Clone
CAT＃: RG235245
- TrueORF®
FLII (tGFP-tagged) - Homo sapiens flightless I homolog (Drosophila) (FLII), transcript variant 2
ORF Plasmid: DDK
"NM_001256264" in other vectors (2)
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CNY 13,780.00
货期*
14周
规格
Product images
经常一起买 (5)
Rabbit Polyclonal antibody to Flightless I (flightless I homolog (Drosophila))
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FLI; Fli1; FLIL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235245 representing NM_001256264
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTCCGAGGCCTCCGCCCAGTGCCTGGCGGCTACTTCCCTGAGAATGTCAAGGCCATGACCAGCC TGCGGTGGCTGAAGCTGAACCGCACTGGCCTCTGCTACCTGCCCGAGGAGCTGGCCGCCCTGCAGAAGCT GGAACACTTGTCTGTGAGCCACAACAACCTGACCACGCTTCATGGGGAGCTGTCCAGCCTGCCATCGCTG CGCGCCATCGTGGCCCGAGCCAACAGTCTGAAGAATTCCGGAGTCCCCGATGACATCTTCAAGCTAGATG ATCTCTCAGTCCTGGACTTGAGCCACAACCAGCTGACAGAGTGCCCGCGGGAGCTGGAGAACGCCAAGAA CATGCTGGTGCTGAACCTCAGCCACAACAGCATCGACACCATCCCCAACCAGCTCTTCATCAACCTCACT GACCTACTATACCTGGACCTCAGCGAGAACCGCCTGGAGAGCCTGCCCCCGCAGATGCGCCGCCTGGTGC ACCTGCAGACGCTCGTGCTCAATGGAAACCCCCTGCTGCATGCACAGCTCCGGCAGCTCCCAGCGATGAC GGCCCTGCAGACCCTGCACCTGCGGAGCACCCAGCGCACCCAGAGCAACCTGCCCACCAGCCTGGAGGGT CTGAGCAACCTCGCAGACGTGGATCTGTCCTGCAATGACCTGACACGGGTGCCCGAGTGTCTGTACACCC TCCCCAGCCTGCGCCGCCTCAACCTCAGCAGCAACCAGATCACGGAGCTGTCCCTGTGCATAGACCAGTG GGTGCACGTGGAAACTCTGAACCTGTCCCGAAATCAGCTCACCTCACTGCCCTCAGCCATTTGCAAGCTG AGCAAGCTGAAGAAGCTGTACCTGAATTCCAACAAGCTGGACTTTGACGGGCTGCCCTCAGGCATTGGCA AGCTCACCAACCTGGAAGAGTTCATGGCTGCCAACAACAACCTGGAGCTGGTCCCTGAAAGTCTCTGCAG GTGCCCAAAGCTGAGGAAACTTGTCCTGAACAAGAACCACCTGGTGACCCTCCCAGAAGCCATCCATTTC CTGACGGAGATCGAGGTCCTGGATGTGCGGGAGAACCCCAACCTGGTCATGCCGCCCAAGCCCGCAGACC GTGCCGCTGAGTGGTACAACATCGACTTCTCGCTGCAGAACCAGCTGCGGCTAGCGGGTGCCTCTCCTGC TACCGTGGCTGCAGCTGCAGCTGCAGGGAGTGGGCCCAAGGACCCTATGGCTCGCAAGATGCGACTGCGG AGGCGCAAGGATTCAGCCCAGGATGACCAGGCCAAGCAGGTGCTGAAGGGCATGTCAGATGTTGCCCAGG AGAAGAACAAAAAGCAGGAGGAGAGCGCAGATGCCCGGGCCCCCAGCGGGAAGGTGCGGCGTTGGGACCA GGGCCTGGAGAAGCCCCGCCTTGACTACTCCGAGTTCTTCACGGAGGACGTGGGCCAGCTGCCCGGACTG ACCATCTGGCAGATAGAGAACTTCGTGCCTGTGCTGGTGGAGGAAGCCTTCCACGGCAAGTTCTACGAGG CTGACTGCTACATTGTGCTCAAGACCTTTCTGGATGACAGCGGCTCCCTCAACTGGGAGATCTACTACTG GATTGGCGGGGAGGCCACACTCGACAAGAAAGCTTGCTCTGCCATCCACGCTGTCAACTTGCGCAACTAC CTGGGTGCTGAGTGCCGCACTGTCCGGGAGGAGATGGGCGATGAGAGCGAGGAGTTCCTGCAGGTGTTTG ACAACGACATCTCCTACATTGAGGGTGGAACAGCCAGTGGCTTCTACACTGTGGAAGACACACACTATGT CACCAGGATGTATCGTGTGTATGGGAAAAAGAACATCAAGTTGGAGCCTGTGCCCCTCAAGGGGACCTCT CTGGACCCAAGGTTTGTTTTCCTGCTGGACCGAGGGCTAGACATCTACGTATGGCGGGGGGCCCAGGCCA CACTGAGCAGCACCACCAAGGCCAGGCTCTTTGCAGAGAAAATTAACAAGAATGAGCGGAAAGGGAAGGC TGAGATCACACTGCTGGTGCAGGGCCAGGAGCTCCCAGAGTTCTGGGAGGCACTGGGTGGGGAGCCCTCT GAGATCAAGAAGCACGTGCCTGAAGACTTCTGGCCGCCGCAGCCCAAGCTGTACAAGGTGGGCCTGGGCT TGGGCTACCTGGAGCTGCCACAGATCAACTACAAGCTCTCCGTGGAACATAAGCAGCGTCCCAAGGTGGA GCTGATGCCAAGAATGCGGCTGCTGCAGAGTCTGCTGGACACGCGCTGCGTGTACATTCTGGACTGTTGG TCCGACGTGTTCATCTGGCTCGGCCGCAAGTCCCCGCGCCTGGTGCGCGCTGCCGCCCTCAAGCTGGGTC AGGAGCTGTGCGGGATGCTGCACCGGCCACGCCATGCCACGGTCAGCCGCAGCCTCGAGGGCACCGAGGC GCAGGTGTTCAAGGCCAAGTTCAAGAATTGGGACGATGTGTTGACGGTGGACTACACACGCAATGCGGAG GCCGTGCTGCAGAGCCCGGGTCTCTCCGGGAAGGTGAAACGCGACGCCGAGAAGAAAGACCAGATGAAGG CTGACCTCACTGCGCTTTTCCTGCCGCGGCAGCCGCCCATGTCGCTGGCCGAGGCGGAGCAGCTGATGGA GGAGTGGAACGAAGACCTAGACGGCATGGAGGGTTTCGTGCTGGAGGGCAAGAAGTTTGCGCGGCTGCCG GAAGAGGAGTTTGGCCACTTCTACACGCAGGACTGCTACGTCTTCCTCTGCAGGTACTGGGTGCCTGTGG AGTACGAGGAGGAGGAAAAGAAGGAAGACAAGGAGGAGAAGGCCGAGGGCAAAGAAGGCGAGGAAGCAAC CGCTGAGGCAGAGGAGAAGCAGCCAGAGGAGGACTTCCAGTGCATCGTGTACTTCTGGCAGGGCCGTGAA GCCTCCAATATGGGCTGGCTCACCTTCACCTTCAGCCTGCAAAAGAAGTTCGAGAGCCTCTTCCCTGGGA AGCTGGAGGTGGTACGCATGACGCAGCAGCAGGAGAACCCCAAGTTCCTGTCCCATTTCAAGAGGAAGTT CATCATCCACCGGGGCAAGAGGAAGGCGGTCCAGGGCGCCCAACAGCCCAGCCTCTACCAGATCCGCACC AACGGCAGCGCCCTCTGCACCCGGTGCATCCAGATCAACACCGACTCCAGCCTCCTCAACTCCGAGTTCT GCTTCATCCTCAAGGTTCCCTTTGAGAGTGAGGACAACCAGGGCATCGTGTATGCCTGGGTGGGCCGGGC ATCAGACCCTGACGAAGCCAAGTTGGCAGAAGACATCCTGAACACCATGTTTGACACCTCCTACAGCAAG CAGGTTATCAACGAAGGTGAGGAGCCTGAGAACTTCTTCTGGGTGGGCATTGGGGCACAGAAGCCCTATG ATGACGATGCCGAGTACATGAAACACACACGTCTCTTCCGGTGCTCCAACGAGAAGGGCTACTTTGCAGT GACTGAGAAATGCTCCGACTTTTGCCAAGATGACCTGGCAGATGATGACATCATGTTGCTAGACAATGGC CAAGAGGTCTACATGTGGGTGGGGACCCAGACTAGCCAGGTGGAGATCAAGCTGAGCCTGAAGGCCTGCC AGGTATATATCCAGCACATGCGGTCCAAGGAACATGAGCGGCCGCGCCGGCTGCGCCTGGTCCGCAAGGG CAATGAGCAGCACGCCTTTACCCGCTGCTTCCACGCCTGGAGCGCCTTCTGCAAGGCCCTGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235245 representing NM_001256264
Red=Cloning site Green=Tags(s) MDLRGLRPVPGGYFPENVKAMTSLRWLKLNRTGLCYLPEELAALQKLEHLSVSHNNLTTLHGELSSLPSL RAIVARANSLKNSGVPDDIFKLDDLSVLDLSHNQLTECPRELENAKNMLVLNLSHNSIDTIPNQLFINLT DLLYLDLSENRLESLPPQMRRLVHLQTLVLNGNPLLHAQLRQLPAMTALQTLHLRSTQRTQSNLPTSLEG LSNLADVDLSCNDLTRVPECLYTLPSLRRLNLSSNQITELSLCIDQWVHVETLNLSRNQLTSLPSAICKL SKLKKLYLNSNKLDFDGLPSGIGKLTNLEEFMAANNNLELVPESLCRCPKLRKLVLNKNHLVTLPEAIHF LTEIEVLDVRENPNLVMPPKPADRAAEWYNIDFSLQNQLRLAGASPATVAAAAAAGSGPKDPMARKMRLR RRKDSAQDDQAKQVLKGMSDVAQEKNKKQEESADARAPSGKVRRWDQGLEKPRLDYSEFFTEDVGQLPGL TIWQIENFVPVLVEEAFHGKFYEADCYIVLKTFLDDSGSLNWEIYYWIGGEATLDKKACSAIHAVNLRNY LGAECRTVREEMGDESEEFLQVFDNDISYIEGGTASGFYTVEDTHYVTRMYRVYGKKNIKLEPVPLKGTS LDPRFVFLLDRGLDIYVWRGAQATLSSTTKARLFAEKINKNERKGKAEITLLVQGQELPEFWEALGGEPS EIKKHVPEDFWPPQPKLYKVGLGLGYLELPQINYKLSVEHKQRPKVELMPRMRLLQSLLDTRCVYILDCW SDVFIWLGRKSPRLVRAAALKLGQELCGMLHRPRHATVSRSLEGTEAQVFKAKFKNWDDVLTVDYTRNAE AVLQSPGLSGKVKRDAEKKDQMKADLTALFLPRQPPMSLAEAEQLMEEWNEDLDGMEGFVLEGKKFARLP EEEFGHFYTQDCYVFLCRYWVPVEYEEEEKKEDKEEKAEGKEGEEATAEAEEKQPEEDFQCIVYFWQGRE ASNMGWLTFTFSLQKKFESLFPGKLEVVRMTQQQENPKFLSHFKRKFIIHRGKRKAVQGAQQPSLYQIRT NGSALCTRCIQINTDSSLLNSEFCFILKVPFESEDNQGIVYAWVGRASDPDEAKLAEDILNTMFDTSYSK QVINEGEEPENFFWVGIGAQKPYDDDAEYMKHTRLFRCSNEKGYFAVTEKCSDFCQDDLADDDIMLLDNG QEVYMWVGTQTSQVEIKLSLKACQVYIQHMRSKEHERPRRLRLVRKGNEQHAFTRCFHAWSAFCKALA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256264 |
| ORF Size | 3774 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256264.2 |
| RefSeq Size | 4208 bp |
| RefSeq ORF | 3777 bp |
| Locus ID | 2314 |
| UniProt ID | Q13045 |
| Protein Families | ES Cell Differentiation/IPS, Transcription Factors |
| Gene Summary | This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
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