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DRIP130 (MED23) (NM_001270521) Human Tagged ORF Clone
CAT＃: RG235279
- TrueORF®
MED23 (tGFP-tagged) - Homo sapiens mediator complex subunit 23 (MED23), transcript variant 3
ORF Plasmid: DDK
"NM_001270521" in other vectors (2)
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CNY 14,920.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARC130; CRSP3; CRSP130; CRSP133; DRIP130; MRT18; SUR-2; SUR2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235279 representing NM_001270521
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACGCAACTGCAGAGCATTTTCGAAGAGGTGGTGAAAACGGAAGTTATAGAAGAGGCTTTTCCTG GCATGTTTATGGATACTCCTGAAGATGAGAAAACAAAACTAATTAGCTGTTTGGGGGCCTTCAGACAGTT TTGGGGTGGTCTTTCTCAGGAGTCTCATGAACAGTGTATCCAGTGGATTGTTAAGTTTATTCATGGTCAG CATAGTCCTAAAAGAATTTCTTTTCTTTATGACTGCTTAGCAATGGCAGTTGAGACTGGTCTCCTTCCAC CCAGGCTGGTTTGTGAATCCCTGATAAACTCTGACACTCTTGAGTGGGAAAGAACACAGCTTTGGGCCTT AACATTTAAACTGGTTCGGAAAATAATTGGGGGAGTGGATTACAAGGGTGTTCGAGATCTCTTAAAAGTG ATTTTGGAGAAGATTTTGACAATTCCTAATACAGTGAGCTCTGCTGTTGTACAGCAGCTTCTGGCAGCAA GAGAGGTTATAGCATATATCTTGGAAAGAAATGCCTGCTTATTACCAGCCTATTTTGCAGTCACTGAGAT CAGGAAACTGTATCCTGAAGGCAAACTTCCACACTGGTTACTTGGAAACCTAGTATCAGACTTTGTGGAT ACCTTCAGGCCCACAGCAAGGATAAACTCCATTTGTGGTCGCTGTAGTCTTCTGCCAGTTGTAAATAATT CGGGTGCCATTTGTAATTCATGGAAACTGGATCCTGCTACTCTTCGTTTTCCTTTGAAAGGCCTTTTGCC ATATGATAAGGATCTGTTTGAACCACAGACTGCTTTGTTGAGATATGTATTGGAGCAGCCTTATTCCAGG GATATGGTCTGCAATATGCTAGGTTTAAATAAGCAGCACAAGCAGCGCTGCCCTGTGCTGGAGGACCAGT TGGTGGATCTGGTTGTTTATGCCATGGAGCGATCTGAGACCGAGGAGAAGTTTGACGATGGGGGAACAAG CCAACTCCTGTGGCAGCATCTCTCAAGTCAGCTCATTTTCTTTGTGCTTTTCCAGTTTGCAAGTTTTCCA CATATGGTGCTTTCTCTTCATCAGAAGTTAGCAGGGCGAGGACTGATTAAAGGCAGAGATCATCTTATGT GGGTTCTCCTGCAATTCATTTCTGGAAGTATTCAGAAAAATGCACTAGCTGATTTTCTCCCTGTGATGAA GCTCTTCGACTTGCTATACCCAGAAAAAGAATATATCCCAGTTCCTGATATTAACAAACCCCAGTCAACC CATGCCTTTGCAATGACCTGTATTTGGATTCATCTCAATAGAAAAGCTCAAAATGACAACTCCAAGCTAC AGATTCCAATACCTCATTCCCTAAGACTTCACCATGAGTTCCTGCAGCAGAGTCTAAGAAATAAAAGTTT ACAGATGAATGACTATAAGATTGCTCTATTGTGTAATGCATACTCTACAAATTCAGAATGTTTTACATTA CCCATGGGAGCTCTGGTAGAAACTATTTATGGAAATGGAATTATGAGGATACCTCTCCCTGGAACAAACT GTATGGCTTCAGGATCTATTACCCCCTTACCTATGAACCTCCTGGATTCACTGACAGTTCATGCCAAAAT GAGCCTTATTCACAGCATTGCAACCAGGGTGATAAAACTTGCTCATGCAAAGTCCAGTGTGGCCTTGGCT CCAGCCCTAGTGGAAACTTACAGTCGTTTATTGGTCTATATGGAAATAGAGTCTTTGGGCATCAAAGGAT TTATCAGTCAGCTTTTGCCAACTGTTTTCAAATCACATGCATGGGGGATCTTACACACACTCCTTGAGAT GTTTAGCTACCGGATGCATCATATTCAGCCTCATTACAGAGTTCAGCTCCTGAGTCATCTTCATACTTTG GCTGCAGTTGCACAAACAAACCAGAACCAGCTCCATCTTTGTGTCGAGAGCACTGCTCTCAGGCTTATAA CAGCATTAGGTAGCTCAGAGGTACAACCGCAGTTTACACGCTTCCTTAGTGATCCCAAAACAGTGCTCTC AGCAGAATCTGAAGAACTGAACCGAGCCTTGATATTGACCTTGGCTAGAGCAACTCATGTAACAGATTTT TTTACAGGCTCTGATTCAATTCAGGGAACTTGGTGTAAAGACATACTTCAGACCATCATGAGTTTCACTC CTCATAATTGGGCTTCACACACCCTGAGCTGTTTTCCAGGCCCACTACAGGCATTCTTCAAACAAAATAA TGTGCCTCAGGAAAGCCGTTTTAATCTGAAAAAAAATGTGGAGGAGGAGTATAGGAAGTGGAAGTCAATG AGCAACGAAAACGACATTATTACCCACTTCTCTATGCAGGGCTCCCCTCCTCTCTTTCTTTGTCTTCTCT GGAAAATGCTCTTGGAAACAGATCATATTAATCAGATTGGCTATAGAGTATTAGAGAGAATTGGAGCCAG GGCCTTGGTAGCCCATGTGAGGACATTTGCAGATTTCCTGGTATATGAGTTTTCTACATCAGCAGGGGGT CAGCAACTCAATAAATGCATTGAAATTCTTAATGACATGGTATGGAAGTATAACATTGTTACACTGGACA GATTAATTCTCTGCCTGGCCATGCGTAGTCACGAAGGAAATGAAGCCCAGGTTTGTTATTTCATAATTCA GTTGCTGTTACTCAAACCAAACGATTTTAGAAATCGAGTAAGTGACTTTGTGAAGGAAAATTCCCCAGAG CACTGGTTACAGAATGACTGGCACACCAAGCACATGAATTATCACAAGAAATATCCAGAGAAGTTGTATT TTGAGGGCCTCGCGGAACAGGTGGATCCTCCTGTACAGATCCAGTCTCCCTATCTGCCCATCTATTTTGG GAATGTGTGTCTTCGATTCCTTCCAGTATTTGATATAGTAATCCACAGATTTTTAGAGTTGCTTCCGGTA TCCAAATCACTGGAGACTCTACTGGATCATCTAGGAGGCTTATATAAATTTCATGATCGTCCAGTGACTT ATCTGTATAACACTCTGCACTATTATGAAATGCACCTGAGAGACCGCGCATTTCTCAAACGAAAACTCGT CCATGCGATCATTGGCTCTCTGAAGGATAATCGACCGCAGGGCTGGTGTCTAAGTGACACTTACCTGAAA TGCGCTATGAATGCACGAGAGGAAAATCCTTGGGTTCCAGATGACACCTACTATTGCAGATTGATTGGCA GACTAGTCGATACGATGGCTGGCAAATCTCCTGGTCCCTTTCCAAACTGTGACTGGAGATTCAATGAGTT TCCCAACCCAGCTGCCCATGCTCTCCATGTTACTTGTGTGGAGCTCATGGCCTTGGCAGTTTCAGGCAAA GAAGTTGGGAATGCCCTTCTAAATGTTGTCCTAAAAAGTCAGCCTTTAGTGCCAAGAGAGAACATTACAG CATGGATGAATGCAATTGGTTTGATCATCACTGCCCTACCAGAGCCATATTGGATTGTTCTTCATGATCG AATTGTGAGTGTCATCAGCAGCCCCAGCTTGACGTCTGAAACAGAGTGGGTTGGCTATCCATTCCGCCTC TTTGATTTCACTGCCTGTCATCAGTCCTACTCTGAGATGAGTTGTAGCTATACGTTAGCTCTTGCACATG CTGTGTGGCACCATTCTAGCATCGGACAACTTTCTCTCATTCCAAAGTTTCTTACTGAAGTACTTCTTCC TATAGTGAAGACCGAATTCCAGTTGCTTTATGTATACCATCTTGTTGGACCATTTTTACAAAGATTTCAG CAAGAGAGAACTCGTTGTATGATAGAGATTGGTGTGGCGTTTTATGACATGCTGCTGAATGTTGACCAGT GTAGCACCCATTTAAATTACATGGATCCCATCTGTGACTTCCTCTATCACATGAAGTATATGTTTACTGG TGACAGCGTGAAAGAGCAAGTAGAGAAGATTATCTGTAACTTAAAACCAGCTTTAAAACTTCGTCTTCGA TTCATCACACACATTAGCAAGATGGAGCCAGCTGCAGTGCCTCCACAAGCCATGAACAGTGGGTCTCCAG CACCTCAGTCTAATCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235279 representing NM_001270521
Red=Cloning site Green=Tags(s) METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCIQWIVKFIHGQ HSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKV ILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVD TFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSR DMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFP HMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQST HAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTL PMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALA PALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTL AAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDF FTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSM SNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGG QQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPE HWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPV SKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLK CAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGK EVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRL FDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQ QERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLR FITHISKMEPAAVPPQAMNSGSPAPQSNQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001270521 |
| ORF Size | 4077 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001270521.2 |
| RefSeq Size | 4612 bp |
| RefSeq ORF | 4080 bp |
| Locus ID | 9439 |
| UniProt ID | Q9ULK4 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2012] |
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