USP19 (NM_001199162) Human Tagged ORF Clone
CAT#: RG235284
- TrueORF®
USP19 (tGFP-tagged) - Homo sapiens ubiquitin specific peptidase 19 (USP19), transcript variant 3
ORF Plasmid: DDK
"NM_001199162" in other vectors (2)
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CNY 15,010.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ZMYND9 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG235284 representing NM_001199162
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCGGGGCCAGTGCCACAGGCCCAAGGAGAGGGCCCCCAGGACTGGAGGACACCACTAGTAAGA AGAAGCAGAAGGATCGAGCAAACCAGGAGAGCAAGGATGGAGATCCTAGGAAAGAGACAGGGTCTCGATA TGTTGCCCAGGCTGGTCTTGAACCTCTGGCCTCAGGTGATCCTTCTGCCTCAGCCTCCCATGCAGCTGGG ATCACAGGCTCACGCCACCGTACCCGGCTGTTCTTTCCTTCATCGTCAGGGTCAGCATCCACTCCTCAAG AGGAGCAGACCAAAGAGGGAGCTTGTGAAGACCCTCATGATCTCTTGGCTACTCCCACTCCAGAGTTGTT GCTCGATTGGAGGCAGAGTGCAGAAGAGGTGATTGTCAAGCTTCGTGTGGGAGTAGGTCCCCTGCAGCTG GAGGATGTAGATGCTGCTTTCACAGATACAGACTGTGTGGTGCGGTTTGCAGGTGGTCAGCAGTGGGGTG GTGTCTTCTATGCTGAGATAAAAAGCTCTTGTGCTAAAGTGCAAACCCGCAAGGGCAGTCTCCTGCACCT GACACTGCCCAAAAAGAAGAAACCTCTAGGGACCCAGGAGCTGGTGCCGGGGCTGCGGTGCCAGGAGAAT GGGCAGGAACTGTCTCCCATTGCCCTGGAGCCAGGCCCTGAGCCCCACCGGGCTAAGCAGGAGGCCCGGA ACCAGAAGCGGGCCCAGGGCCGTGGTGAGGTAGGCGCAGGGGCTGGCCCCGGGGCCCAGGCAGGGCCCAG CGCCAAGAGGGCTGTGCATCTCTGCAGAGGGCCAGAGGGGGACGGGTCCAGGGATGACCCTGGACCCCGG GGTGATGCCCCACCCTTCGTGGCTGACCCAGCCACCCAGGTTGAGGCTGATGAACAGCTTTGCATACCAC CGCTGAACTCCCAAACCTGCCTCCTGGGCTCAGAGGAGAATTTAGCCCCTTTGGCAGGAGAGAAAGCAGT GCCTCCCGGGAATGACCCAGTCTCTCCAGCCATGGTCCGGAGCAGAAACCCTGGGAAAGATGACTGTGCC AAGGAGGAGATGGCAGTGGCAGCAGATGCTGCAACCTTGGTGGATGAGCCCGAGTCGATGGTGAACCTGG CGTTTGTCAAGAATGACTCGTATGAGAAGGGCCCGGATTCAGTGGTGGTGCACGTGTACGTGAAGGAGAT CTGCAGGGACACCTCAAGAGTACTTTTCCGTGAGCAGGACTTCACGCTCATCTTCCAGACCAGGGATGGA AACTTCCTGAGGCTGCACCCGGGCTGTGGGCCCCACACCACCTTCCGTTGGCAGGTGAAGCTCAGGAATC TGATTGAGCCAGAGCAGTGCACCTTCTGTTTCACGGCTTCTCGCATCGACATCTGCCTTCGTAAGAGGCA GAGTCAGCGCTGGGGGGGCCTGGAGGCCCCGGCTGCACGAGTGGGTGGTGCAAAGGTTGCCGTGCCGACA GGTCCAACCCCTCTGGATTCAACCCCACCAGGAGGTGCTCCCCACCCCCTGACAGGCCAGGAGGAGGCCC GGGCTGTGGAGAAGGATAAATCCAAGGCACGATCTGAGGACACAGGGCTAGACAGTGTGGCAACCCGCAC ACCCATGGAGCATGTAACCCCAAAGCCAGAGACACACCTGGCCTCGCCCAAGCCTACATGCATGGTGCCT CCCATGCCCCACAGCCCAGTTAGTGGAGACAGCGTGGAGGAGGAGGAAGAGGAAGAGAAGAAGGTGTGTC TGCCAGGCTTCACTGGCCTTGTCAATTTAGGCAACACCTGCTTCATGAACAGCGTCATTCAGTCTCTGTC CAACACTCGGGAACTCCGGGACTTCTTCCATGACCGCTCCTTTGAGGCTGAGATCAACTACAACAACCCA CTAGGGACTGGTGGGCGTCTGGCCATTGGCTTTGCCGTGCTGCTTCGGGCGCTGTGGAAGGGCACCCACC ATGCCTTCCAGCCTTCCAAGTTGAAGGCCATTGTGGCGAGTAAGGCCAGCCAGTTCACAGGCTATGCACA GCATGATGCCCAGGAGTTCATGGCTTTCCTGCTGGATGGGCTGCACGAGGACCTGAATCGCATTCAGAAC AAGCCCTACACAGAGACCGTGGATTCAGATGGGCGGCCCGATGAGGTGGTAGCTGAGGAAGCATGGCAGC GGCACAAGATGAGGAATGACTCTTTCATCGTGGACCTATTTCAGGGGCAGTACAAGTCGAAGCTGGTGTG CCCTGTGTGTGCCAAGGTCTCCATCACTTTTGACCCGTTTCTTTATCTGCCGGTGCCCTTGCCACAAAAG CAAAAGGTTCTCCCTGTCTTTTATTTTGCCCGAGAGCCCCACAGCAAGCCCATCAAGTTCCTGGTGAGCG TCAGCAAGGAGAACTCCACTGCGAGCGAAGTATTGGACTCCCTCTCTCAGAGTGTTCATGTGAAGCCTGA GAACCTGCGTTTGGCGGAGGTAATTAAGAATCGTTTTCATCGTGTGTTCCTACCCTCCCACTCACTGGAC ACTGTGTCCCCATCTGATACGCTCCTCTGCTTTGAGCTGCTATCCTCAGAGTTGGCTAAGGAGCGGGTAG TGGTGCTAGAGGTGCAACAGCGCCCCCAGGTGCCCAGCGTCCCCATCTCCAAGTGTGCAGCCTGCCAGCG GAAGCAACAGTCGGAGGATGAAAAGCTGAAGCGCTGTACCCGGTGCTACCGTGTGGGCTACTGCAACCAG CTCTGCCAGAAAACCCACTGGCCTGACCACAAGGGCCTCTGCCGACCTGAGAACATTGGCTACCCCTTCC TGGTCAGTGTACCTGCCTCACGCCTCACTTATGCCCGCCTCGCTCAGTTGCTAGAGGGCTATGCCCGGTA CTCTGTGAGTGTATTCCAGCCACCCTTTCAGCCAGGCCGCATGGCCTTGGAGTCTCAGAGCCCTGGCTGC ACCACACTGCTCTCCACAGGTTCCCTGGAGGCTGGGGACAGCGAGAGAGACCCCATTCAGCCACCTGAGC TCCAGCTGGTGACCCCTATGGCTGAGGGGGACACAGGGCTTCCCCGGGTGTGGGCAGCCCCTGACCGGGG TCCTGTGCCCAGCACCAGTGGAATTTCTTCTGAGATGCTGGCCAGTGGGCCCATTGAGGTTGGCTCCTTG CCAGCTGGCGAGAGGGTGTCCCGACCCGAAGCTGCTGTGCCTGGGTACCAGCATCCAAGTGAAGCTATGA ATGCCCACACACCCCAGTTCTTCATCTATAAAATTGATTCATCCAACCGAGAGCAGCGGCTAGAGGACAA AGGAGACACCCCACTGGAGCTGGGTGACGACTGTAGCCTGGCTCTCGTCTGGCGGAACAATGAGCGCTTG CAGGAGTTTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAGGATCCAGGCTCTGCCGGTGAGGCTG CCCGGGCCGGCCACTTCACCCTGGACCAGTGCCTCAACCTCTTCACACGGCCTGAGGTGCTGGCACCCGA GGAGGCCTGGTACTGCCCACAGTGCAAACAGCACCGTGAGGCCTCCAAGCAGCTGTTGCTATGGCGCCTG CCAAATGTTCTCATCGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTTTATCTGGCGTGACAAGATCAATG ACTTGGTGGAGTTCCCTGTTAGGAACCTGGACCTGAGCAAGTTCTGCATTGGTCAGAAAGAGGAGCAGCT GCCCAGCTACGATCTATATGCTGTCATCAACCACTATGGAGGCATGATTGGTGGCCACTACACTGCCTGT GCACGCCTGCCCAATGATCGTAGCAGTCAGCGCAGTGACGTGGGCTGGCGCTTGTTTGATGACAGCACAG TGACAACGGTAGACGAGAGCCAGGTTGTGACGCGTTATGCCTATGTACTCTTCTACCGCCGGCGGAACTC TCCTGTGGAGAGGCCCCCCAGGGCAGGTCACTCTGAGCACCACCCAGACCTAGGCCCTGCAGCTGAGGCT GCTGCCAGCCAGGGACTAGGCCCTGGCCAGGCCCCCGAGGTGGCCCCCACGCGGACAGCCCCTGAACGCT TCGCCCCCCCTGTGGATCGGCCAGCCCCCACCTACAGCAACATGGAGGAGGTGGAT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235284 representing NM_001199162
Red=Cloning site Green=Tags(s) MSGGASATGPRRGPPGLEDTTSKKKQKDRANQESKDGDPRKETGSRYVAQAGLEPLASGDPSASASHAAG ITGSRHRTRLFFPSSSGSASTPQEEQTKEGACEDPHDLLATPTPELLLDWRQSAEEVIVKLRVGVGPLQL EDVDAAFTDTDCVVRFAGGQQWGGVFYAEIKSSCAKVQTRKGSLLHLTLPKKKKPLGTQELVPGLRCQEN GQELSPIALEPGPEPHRAKQEARNQKRAQGRGEVGAGAGPGAQAGPSAKRAVHLCRGPEGDGSRDDPGPR GDAPPFVADPATQVEADEQLCIPPLNSQTCLLGSEENLAPLAGEKAVPPGNDPVSPAMVRSRNPGKDDCA KEEMAVAADAATLVDEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEICRDTSRVLFREQDFTLIFQTRDG NFLRLHPGCGPHTTFRWQVKLRNLIEPEQCTFCFTASRIDICLRKRQSQRWGGLEAPAARVGGAKVAVPT GPTPLDSTPPGGAPHPLTGQEEARAVEKDKSKARSEDTGLDSVATRTPMEHVTPKPETHLASPKPTCMVP PMPHSPVSGDSVEEEEEEEKKVCLPGFTGLVNLGNTCFMNSVIQSLSNTRELRDFFHDRSFEAEINYNNP LGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQN KPYTETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPVCAKVSITFDPFLYLPVPLPQK QKVLPVFYFAREPHSKPIKFLVSVSKENSTASEVLDSLSQSVHVKPENLRLAEVIKNRFHRVFLPSHSLD TVSPSDTLLCFELLSSELAKERVVVLEVQQRPQVPSVPISKCAACQRKQQSEDEKLKRCTRCYRVGYCNQ LCQKTHWPDHKGLCRPENIGYPFLVSVPASRLTYARLAQLLEGYARYSVSVFQPPFQPGRMALESQSPGC TTLLSTGSLEAGDSERDPIQPPELQLVTPMAEGDTGLPRVWAAPDRGPVPSTSGISSEMLASGPIEVGSL PAGERVSRPEAAVPGYQHPSEAMNAHTPQFFIYKIDSSNREQRLEDKGDTPLELGDDCSLALVWRNNERL QEFVLVASKELECAEDPGSAGEAARAGHFTLDQCLNLFTRPEVLAPEEAWYCPQCKQHREASKQLLLWRL PNVLIVQLKRFSFRSFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLPSYDLYAVINHYGGMIGGHYTAC ARLPNDRSSQRSDVGWRLFDDSTVTTVDESQVVTRYAYVLFYRRRNSPVERPPRAGHSEHHPDLGPAAEA AASQGLGPGQAPEVAPTRTAPERFAPPVDRPAPTYSNMEEVD SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001199162 |
ORF Size | 4116 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001199162.1, NP_001186091.1 |
RefSeq Size | 4847 bp |
RefSeq ORF | 4119 bp |
Locus ID | 10869 |
UniProt ID | O94966 |
Protein Families | Druggable Genome, Protease, Transmembrane |
Gene Summary | Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This protein is a ubiquitin protein ligase and plays a role in muscle wasting. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2017] |
Documents
Product Manuals |
FAQs |
SDS |