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COL8A2 (NM_001294347) Human Tagged ORF Clone
CAT＃: RG239174
- TrueORF®
COL8A2 (tGFP-tagged) - Human collagen, type VIII, alpha 2 (COL8A2), transcript variant 2
ORF Plasmid: DDK
"NM_001294347" in other vectors (2)
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CNY 4,940.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FECD; FECD1; PPCD; PPCD2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239174 representing NM_001294347.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCTCTACCGCTGCTGCCGATGGACCTGAAGGGAGAGCCCGGCCCCCCTGGGAAGCCCGGGCCTCGG GGTCCCCCTGGCCCCCCTGGCTTCCCAGGAAAACCAGGCATGGGAAAGCCAGGACTCCATGGGCAGCCT GGCCCTGCTGGGCCCCCTGGCTTCTCCCGGATGGGCAAGGCTGGTCCCCCAGGGCTCCCTGGCAAGGTC GGGCCACCAGGGCAGCCGGGGCTTCGGGGGGAGCCAGGAATACGAGGGGACCAGGGCCTCCGGGGACCC CCAGGACCCCCTGGCCTCCCGGGCCCCTCAGGCATTACTATCCCTGGAAAACCAGGTGCCCAAGGGGTG CCAGGGCCCCCAGGATTCCAGGGGGAACCAGGGCCCCAGGGGGAGCCTGGGCCCCCAGGTGATCGAGGC CTCAAGGGGGATAATGGAGTGGGCCAGCCCGGGCTGCCTGGGGCCCCAGGGCAGGGGGGTGCCCCCGGC CCCCCCGGCCTCCCTGGTCCAGCTGGCTTAGGCAAACCTGGTTTGGATGGGCTTCCTGGGGCCCCAGGA GACAAGGGTGAGTCTGGGCCTCCTGGAGTTCCAGGCCCCAGGGGGGAGCCAGGAGCTGTGGGCCCAAAA GGACCTCCTGGAGTAGACGGTGTGGGAGTCCCAGGGGCAGCAGGGTTGCCAGGACCACAGGGCCCATCA GGGGCCAAAGGGGAGCCAGGGACCCGGGGCCCCCCTGGGCTGATAGGCCCCACTGGCTATGGGATGCCA GGACTGCCAGGCCCCAAGGGGGACAGGGGCCCAGCTGGGGTCCCAGGACTCTTGGGGGACAGGGGTGAG CCAGGGGAGGATGGGGAGCCAGGGGAGCAGGGCCCACAGGGTCTTGGGGGTCCCCCTGGACTTCCTGGG TCTGCAGGGCTTCCTGGCAGACGTGGGCCCCCTGGGCCTAAGGGTGAGGCAGGGCCTGGAGGACCCCCA GGAGTGCCTGGCATTCGAGGTGACCAGGGGCCTAGTGGCCTGGCTGGGAAACCAGGGGTCCCAGGTGAG AGGGGACTTCCTGGGGCCCATGGACCCCCTGGACCAACTGGGCCCAAGGGTGAGCCGGGTTTCACGGGT CGCCCTGGAGGACCAGGGGTGGCAGGAGCCCTGGGGCAGAAAGGTGACTTGGGGCTCCCTGGGCAGCCT GGCCTGAGGGGTCCCTCAGGAATCCCAGGACTCCAGGGTCCAGCTGGCCCTATTGGGCCCCAAGGCCTG CCGGGCCTGAAGGGGGAACCAGGCCTGCCAGGGCCCCCTGGAGAGGGGAGAGCAGGGGAACCTGGCACG GCTGGGCCCACGGGGCCCCCAGGGGTCCCTGGCTCCCCTGGAATCACGGGCCCTCCGGGGCCTCCCGGG CCCCCGGGACCCCCTGGTGCCCCTGGGGCCTTCGATGAGACTGGCATCGCAGGCTTGCACCTGCCCAAC GGCGGTGTGGAGGGTGCCGTGCTGGGCAAGGGGGGCAAGCCACAGTTTGGGCTGGGCGAGCTGTCTGCC CATGCCACACCGGCCTTCACTGCGGTGCTCACCTCGCCCTTCCCCGCCTCGGGCATGCCCGTGAAATTT GACCGGACTCTCTACAATGGCCACAGCGGCTACAACCCAGCCACTGGCATCTTCACCTGCCCTGTGGGC GGCGTCTACTACTTTGCTTACCATGTGCACGTCAAGGGCACCAACGTGTGGGTGGCCCTGTACAAGAAC AACGTGCCGGCCACCTATACCTACGATGAGTACAAGAAGGGCTACCTGGACCAGGCATCTGGTGGGGCC GTGCTCCAGCTGCGGCCCAACGACCAGGTCTGGGTGCAGATGCCGTCGGACCAGGCCAACGGCCTCTAC TCCACGGAGTACATCCACTCCTCCTTTTCAGGATTCTTGCTCTGCCCCACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239174
Blue=ORF Red=Cloning site Green=Tag(s) MPLPLLPMDLKGEPGPPGKPGPRGPPGPPGFPGKPGMGKPGLHGQPGPAGPPGFSRMGKAGPPGLPGKV GPPGQPGLRGEPGIRGDQGLRGPPGPPGLPGPSGITIPGKPGAQGVPGPPGFQGEPGPQGEPGPPGDRG LKGDNGVGQPGLPGAPGQGGAPGPPGLPGPAGLGKPGLDGLPGAPGDKGESGPPGVPGPRGEPGAVGPK GPPGVDGVGVPGAAGLPGPQGPSGAKGEPGTRGPPGLIGPTGYGMPGLPGPKGDRGPAGVPGLLGDRGE PGEDGEPGEQGPQGLGGPPGLPGSAGLPGRRGPPGPKGEAGPGGPPGVPGIRGDQGPSGLAGKPGVPGE RGLPGAHGPPGPTGPKGEPGFTGRPGGPGVAGALGQKGDLGLPGQPGLRGPSGIPGLQGPAGPIGPQGL PGLKGEPGLPGPPGEGRAGEPGTAGPTGPPGVPGSPGITGPPGPPGPPGPPGAPGAFDETGIAGLHLPN GGVEGAVLGKGGKPQFGLGELSAHATPAFTAVLTSPFPASGMPVKFDRTLYNGHSGYNPATGIFTCPVG GVYYFAYHVHVKGTNVWVALYKNNVPATYTYDEYKKGYLDQASGGAVLQLRPNDQVWVQMPSDQANGLY STEYIHSSFSGFLLCPT TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001294347 |
| ORF Size | 1914 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001294347.2 |
| RefSeq Size | 4397 bp |
| RefSeq ORF | 1917 bp |
| Locus ID | 1296 |
| UniProt ID | P25067 |
| Protein Families | Druggable Genome |
| MW | 61.2 kDa |
| Gene Summary | This gene encodes the alpha 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
Documents
| Product Manuals |
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