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USP25 (NM_001283041) Human Tagged ORF Clone
CAT＃: RG239986
- TrueORF®
USP25 (tGFP-tagged) - Human ubiquitin specific peptidase 25 (USP25), transcript variant 1
ORF Plasmid: DDK
"NM_001283041" in other vectors (2)
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CNY 11,590.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | USP21 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239986 representing NM_001283041.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTTGAATCAA CTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAAC TTGGAATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGAGGAGACAACTTAC TACCAAACAGCACTTCCTGGCAATGATAGATACATCAGTGTGGGAAGCCAAGCAGATACAAATGTGATT GATCTCACTGGAGATGATAAAGATGATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAGG GCATTCAGGGAGACTGGAATAACTGATGAGGAACAAGCCATTAGCAGAGTTCTTGAAGCCAGCATAGCA GAGAATAAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGA AAAAGACAGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTTAGTGCTGTTATT CAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTGAATTACAAGCCTCCATCAAATGCTCAA GATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCTTTTATGCGTGAGCTGAGGTATCTATTTGCA CTTCTTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTC AAATCAAATGACTCACAGCAGCAAGATGTGAGTGAGTTTACACACAAATTATTAGATTGGTTAGAAGAT GCCTTCCAAATGAAAGCTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTGTTC TATGGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGAAAACACTGAAATGTTTGGTCAG TACCCACTTCAGGTCAATGGGTTCAAAGATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAAGGAGAA ATTGAGTCTTTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGAATTACCACCT GTGTTAACATTTGAATTGTCAAGATTTGAATTTAATCAGGCATTGGGAAGACCAGAAAAAATTCACAAC AAATTAGAATTTCCCCAAGTTTTATATTTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATT AAGAGGGAAGAGATCAAGAGACTGAAAGATTACCTCACGGTATTACAACAAAGGCTAGAAAGATATTTA AGCTATGGTTCCGGTCCCAAACGATTCCCCTTGGTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCA AGTAAACCTGTTTGCACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCATACCA TCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCTTCAGAACTGCCAAGCACATCACCT TCATCAGTTGCTGCCATTTCATCGAGATCAGTAATACACAAACCATTTACTCAGTCCCGGATACCTCCA GATTTGCCCATGCATCCGGCACCAAGGCACATAACGGAGGAAGAACTTTCTGTGCTGGAAAGTTGTTTA CATCGCTGGAGGACAGAAATAGAAAATGACACCAGAGATTTGCAGGAAAGCATATCCAGAATCCATCGA ACAATTGAATTAATGTACTCTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTT CACGAAGGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCATCGTGAAAGCAGATGGATG AAGTACAATGATATTGCTGTGACAAAATCATCATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGTTAT AGAAATGCCAGTGCATACTGTTTAATGTACATAAATGATAAGGCACAGTTCCTAATACAAGAGGAGTTT AATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACATTACCACCGGATTTGAGAGATTTTGTTGAG GAAGACAACCAACGATTTGAAAAAGAACTAGAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAG GAAAAGCTTTTAGCGTCTCAGAAATTGAGAGAGTCAGAGACTTCTGTGACAACAGCACAAGCAGCAGGA GACCCAGAATATCTAGAGCAGCCATCAAGAAGTGATTTCTCAAAGCACTTGAAAGAAGAAACTATTCAA ATAATTACCAAGGCATCACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGAAACCTGAA AATACTACAAGCCAACCACTTTCTAATCAGCGAGTTGTAGAGGTGGCGATCCCTCATGTAGGGAAATTT ATGATTGAATCAAAGGAGGGGGGGTATGATGACGAGATCATGATGACACCGAACATGCAAGGTATTATC ATGGCGATAGGTAAATCCAGGAGTGTATATGACAGGTGTGGCCCTGAAGCAGGGTTCTTTAAGGCAATT AAGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCAGAAACCGATTATCGTTTA CATCATGTAGTGGTCTACTTTATCCAGAACCAGGCACCAAAGAAAATTATTGAGAAAACATTACTAGAA CAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGTCACAACATAATGAAAGTTGCTCAAGCCAAA CTGGAAATGATAAAACCTGAAGAAGTAAACTTGGAGGAATATGAGGAGTGGCATCAGGATTATAGGAAA TTCAGGGAAACAACTATGTATCTCATAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCC TTGCTGTTCCTCATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTTATACAGAGGACAT GATGAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTAAAATTAAATGAGCAAGCCGCAGAACTC TTCGAATCTGGAGAGGATCGAGAAGTAAACAATGGTTTGATTATCATGAATGAGTTTATTGTCCCATTT TTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTAGCTGTAGAAGATATGAGAAATCGA TGGTGTTCCTACCTTGGTCAAGAAATGGAACCACACCTCCAAGAAAAGCTGACAGATTTTTTGCCAAAA CTGCTTGATTGTTCTATGGAGATTAAAAGTTTCCATGAGCCACCGAAGTTACCTTCATATTCCACGCAT GAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGTCGAACTCCTGCTGATGGAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239986
Blue=ORF Red=Cloning site Green=Tag(s) MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTY YQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIA ENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQ DLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLED AFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGE IESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRI KREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIP SQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCL HRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWM KYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVE EDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQ IITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGII MAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLE QFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDS LLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPF LPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTH ELCERFARIMLSLSRTPADGR TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001283041 |
| ORF Size | 3375 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001283041.2 |
| RefSeq Size | 5359 bp |
| RefSeq ORF | 3378 bp |
| Locus ID | 29761 |
| UniProt ID | Q9UHP3 |
| Protein Families | Protease |
| MW | 130.4 kDa |
| Gene Summary | Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008] |
Documents
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