Usp9x (NM_001135893) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1560056
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200044 representing NM_001135893 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCCACGACTCGTGGCTCTCCAGTTGGAGGGAATGACAACCAGGGCCAAGCTCCTGATGGACAGT CTCAGCCCCCCCTCCAACAGAATCAGACTTCATCGCCTGATTCATCCAATGAAAATTCCCCTGCAACTCC TCCTGATGAGCAAGGCCAAGGTGATGCTCCTCCCCAGATTGAAGATGAGGAACCTGCATTTCCACACACT GACCTGGCAAAGTTAGATGATATGATCAACAGGCCTCGCTGGGTCGTTCCAGTTTTGCCGAAAGGGGAAT TAGAAGTGCTTTTAGAAGCTGCTATTGATCTTAGTAAGAAAGGCCTTGATGTCAAAAGTGAAGCATGTCA GAGATTTTTCCGAGATGGGCTAACAATCTCATTCACAAAAATCCTTACAGATGAAGCAGTGAGTGGCTGG AAGTTTGAAATTCATAGATGTATTATTAACAATACTCATCGCCTGGTGGAGCTGTGTGTGGCTAAGTTAG CCCAAGACTGGTTTCCACTTCTAGAACTTCTTGCCATGGCCTTAAATCCTCATTGCAAATTCCATATCTA CAATGGTACACGTCCCTGCGAGTCAGTGTCCTCAAGTGTTCAGTTGCCTGAAGATGAACTCTTTGCTCGT TCTCCAGACCCTCGATCACCAAAAGGTTGGCTAGTGGATCTCCTCAACAAATTTGGCACTTTAAATGGAT TCCAGATACTGCATGATCGTTTTATTAATGGATCAGCATTAAATGTTCAGATAATTGCAGCCCTTATTAA ACCATTTGGACAGTGCTATGAGTTTCTTACTCTTCACACGGTGAAAAAATACTTTCTTCCAATAATAGAA ATGGTTCCACAGTTTTTAGAAAACTTAACTGATGAAGAGCTGAAGAAAGAAGCAAAGAATGAAGCCAAAA ATGATGCTCTCTCAATGATTATTAAATCATTGAAGAGTTTAGCTTCACGAGTTCCTGGACAGGAAGAAAC TGTAAAGAACTTAGAAATATTTAGGTTAAAAATGATACTTAGATTATTGCAAATTTCTTCCTTCAATGGA AAGATGAATGCACTGAATGAGGTTAATAAGGTGATTTCCAGTGTGTCATACTATACCCATCGGCATGGCA GTTCTGAGGAAGAAGAGTGGCTCACAGCAGAGAGGATGGCTGAATGGATACAGCAGAACAATATTTTATC AATAGTTTTACGAGATAGTCTTCATCAACCACAGTATGTAGAGAAACTAGAAAAGATTCTTCGCTTTGTC ATAAAAGAAAAAGCTCTGACCTTGCAAGATCTTGATAATATCTGGGCAGCACAGGCAGGGAAACATGAAG CCATCGTAAAGAATGTCCATGATCTACTGGCCAAATTGGCATGGGATTTTTCTCCTGAACAACTTGATCA TCTTTTTGATTGCTTTAAGGCCAGTTGGACAAATGCAAGTAAAAAGCAACGTGAAAAGCTCCTTGAGCTG ATTCGTCGTCTTGCAGAAGATGATAAAGATGGTGTGATGGCACATAAAGTGTTGAACCTTCTGTGGAATC TAGCTCACAGTGATGATGTGCCTGTGGATATCATGGACTTGGCTCTCAGTGCTCACATCAAAATACTAGA TTATAGTTGCTCCCAGGACCGGGACACACAAAAGATCCAGTGGATAGATCGCTTCATAGAAGAACTTCGC ACAAATGACAAGTGGGTCATTCCTGCACTGAAACAAATTAGAGAAATTTGCAGTTTGTTTGGTGAAGCAC CTCAAAATTTGAGTCAAACTCAGAGAAGTCCCCATGTATTTTATCGCCATGATTTAATCAATCAACTTCA GCACAATCATGCCCTAGTTACTTTGGTAGCAGAAAACCTTGCAACTTACATGGAAAGCATGAGACTGTAT GGCAGAGACAATGAAGACTATGACCCACAAACTGTGAGAGTGGGAAGTAGATATAGTCATGTTCAAGAAG TCCAAGAACGTCTTAACTTTCTTAGATTTTTATTGAAGGATGGCCAGCTATGGCTGTGTGCTCCTCAGGC AAAACAAATATGGAAGTGCTTAGCAGAGAATGCGGTTTATCTTTGTGACCGTGAAGCCTGCTTTAAGTGG TATTCCAAATTAATGGGAGATGAACCAGACTTAGATCCTGATATCAATAAGGACTTCTTTGAAAGTAATG TGCTTCAGCTTGATCCTTCACTATTAACTGAAAATGGAATGAAATGTTTTGAGAGATTCTTCAAAGCTGT GAATTGTCGAGAAGGAAAACTAGTAGCAAAAAGAAGAGCCTATATGATGGATGATTTGGAATTGATAGGA TTAGACTATCTTTGGAGGGTTGTAATTCAGAGTAATGATGATATTGCCAGCCGAGCTATTGATCTCCTCA AAGAGATATACACAAACCTTGGTCCAAGACTGCAGGTCAATCAGGTGGTGATCCATGAAGACTTCATTCA GTCTTGCTTTGATCGTTTGAAAGCCTCTTATGACACGTTGTGTGTTTTGGATGGTGACAAAGACAGTATT AATTGTGCAAGACAGGAAGCTGTTCGAATGGTCCGAGTATTAACTGTTTTAAGAGAATATATAAATGAAT GTGACAGTGATTATCATGAAGAAAGAACAATTTTGCCTATGTCAAGAGCTTTCCGTGGTAAACACCTCTC TTTTATAGTTCGGTTTCCAAACCAGGGCAGACAAGTAGATGACTTGGAAGTTTGGTCTCATACAAATGAT ACCATTGGTTCAGTTCGACGATGTATACTAAATCGCATTAAAGCCAATGTAGCTCATACAAAAATTGAAC TCTTTGTGGGTGGTGAGCTTATTGATCCTGGAGATGACAGAAAGTTGATTGGACAATTAAACCTAAAAGA TAAATCACTAATTACAGCCAAACTTACACAAATCAGCTCCAATATGCCGTCAAGTCCTGATAGCTCCTCT GATTCCTCAACTGGATCTCCTGGAAACCATGGTAATCATTACAGTGATGGTCCCAACCCAGAGGTAGAAA GCTGTTTGCCTGGAGTGATAATGTCACTTCATCCCAGATACATCTCTTTCCTTTGGCAAGTTGCAGACTT AGGGAGCAGCCTAAATATGCCCCCTCTTAGAGATGGAGCAAGAGTTCTTATGAAACTTATGCCGCCAGAT AGTACAACAATAGAAAAATTAAGAGCTATTTGTTTAGATCATGCCAAACTTGGAGAAAGCAGCCTTAGTC CATCTCTTGACTCACTTTTCTTTGGTCCTTCAGCCTCACAAGTGCTATACCTAACAGAGGTAGTCTATGC CCTGTTAATGCCTGCTGGTGCACCCCTGGCTGATGACTCCTCTGATTTTCAGTTTCACTTCTTGAAAAGT GGTGGTTTGCCCCTTGTTCTGAGTATGCTAACCAGAAATAACTTCCTACCAAATGCTGATATGGAAACTC GAAGGGGTGCCTACCTCAATGCTCTTAAAATAGCCAAACTGTTACTAACTGCCATTGGCTATGGCCATGT TCGGGCTGTGGCAGAAGCATGTCAGCCAGGTGTAGAAGGCGTGAATCCTATGACATCGGTCAACCAAGTA ACTCATGATCAAGCAGTGGTGCTACAAAGTGCCCTTCAGAGCATTCCTAACCCATCATCTGAATGCATGC TTAGAAACGTGTCTGTTCGTCTTGCTCAGCAGATTTCTGATGAGGCTTCAAGATATATGCCTGATATTTG TGTAATTAGAGCTATACAAAAGATTATTTGGACATCAGGATGTGGGGGATTACAACTGGTATTCAGCCCA AATGAGGAAGTCACAAAAATTTATGAGAAGACCAATGCAGGCAATGAGCCAGACCTTGAAGATGAACAGG TTTGCTGTGAGGCATTGGAAGTGATGACACTGTGTTTCGCCCTGATTCCAACAGCCTTAGATGCTCTAAG TAAAGAAAAGGCTTGGCAGACATTTATTATTGACTTACTGTTACACTGTCACAGCAAAACTGTCCGTCAA GTAGCACAGGAACAATTCTTCTTAATGTGCACCAGATGTTGCATGGGACACAGGCCTCTACTTTTCTTCA TTACTCTACTCTTTACTGTTTTGGGGAGCACAGCCAGAGAGAGGGCTAAACATTCAGGCGACTACTTTAC TCTTTTAAGGCATCTTCTTAATTACGCTTACAACAGTAACATTAATGTACCCAATGCTGAAGTTCTTCTA AATAATGAAATTGATTGGCTTAAAAGAATTAGGGATGATGTTAAAAGAACAGGTGAAACAGGCGTTGAAG AGACAATCTTAGAAGGACACCTTGGGGTTACAAAGGAGCTACTGGCTTTTCAGACCCCTGAGAAAAAGTT TCATATTGGTTGTGAAAAGGGGGGTGCTAATCTCATTAAAGAATTAATCGACGATTTCATCTTTCCTGCA TCCAATGTTTACCTCCAGTATATGAGAAATGGAGAACTCCCTGCTGAGCAGGCCATTCCTGTCTGTGGTT CACCAGCCACAATTAATGCTGGTTTTGAGTTACTTGTAGCATTAGCTGTTGGCTGTGTAAGGAACCTCAA ACAGATAGTAGATTCTTTGACTGAAATGTATTACATTGGTACAGCAATAACTACTTGTGAGGCACTTACT GAGTGGGAATACCTGCCACCTGTTGGACCCCGTCCACCAAAAGGATTTGTGGGGCTGAAAAATGCTGGTG CTACTTGTTACATGAATTCTGTGATTCAGCAACTCTATATGATTCCCTCTATCAGGAACGGTATTCTTGC AATTGAAGGCACAGGTAGTGATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAATGAGAGCAAT GTTGATCCCAGGGATGATGTGTTTGGATATCCTCAACAATTTGAAGACAAACCACCACTGAGTAAAACAG AAGATAGAAAAGAGTACAATATTGGTGTTCTAAGACACCTTCAAGTTATCTTTGGCCATTTAGCTGCTTC TCGACTACAGTACTATGTGCCCAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTTAATCTT CGTGAACAACATGATGCTTTAGAGTTCTTTAATTCATTGGTGGATAGTTTAGATGAAGCTTTAAAAGCCT TAGGGCATCCAGCTATGCTAAGTAAAGTCTTGGGAGGTTCCTTCGCTGATCAGAAAATTTGTCAAGGCTG CCCACATAGGTATGAATGTGAAGAATCTTTTACGACTCTGAATGTAGACATTAGAAACCACCAAAATCTT CTTGATTCTTTGGAACAGTATGTCAAAGGAGATTTACTAGAAGGGGCAAATGCTTATCATTGTGAAAAAT GCAATAAAAAGGTTGATACTGTAAAGCGCTTGCTAATTAAAAAGTTGCCTCCTGTTCTTGCTATCCAACT AAAACGATTCGATTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTACTTTGAGTTTCCTCGA GAGTTAGACATGGAGCCGTACACAGTTGCAGGTGTTGCTAAACTAGAGGGAGATAATGTCAACCCAGAAA GTCAGCTGATACAACAGAATGAGCAGTCTGAAAGCGAAAAAGCCGGAAGCACAAAATACAGACTTGTGGG TGTGCTTGTACACAGTGGTCAAGCAAGTGGGGGACATTACTATTCTTACATCATTCAGAGGAATGGAGGA GATGGTGAAAAAAATCGTTGGTATAAATTTGATGATGGAGATGTAACAGAGTGTAAAATGGATGATGATG AAGAAATGAAAAACCAGTGTTTTGGTGGCGAGTACATGGGAGAAGTGTTTGATCACATGATGAAGCGCAT GTCGTACAGGCGGCAGAAAAGGTGGTGGAATGCATATATACTTTTTTATGAAAGAATGGATACGATAGAT CATGATGATGAGGTGATAAGATACATATCAGAGATTGCTATCACCACAAGGCCCCATCAGATTGTTATGC CATCGGCCATTGAAAGAAGTGTGCGGAAGCAGAATGTCCAATTCATGCATAACCGAATGCAGTACAGTTT GGAATATTTTCAGTTTATGAAAAAGCTACTTACATGTAATGGTGTTTATTTAAACCCTCCACCAGGGCAA GATCACCTGTCTCCTGAAGCAGAAGAAATCACTATGATTAGTATTCAGCTTGCTGCTAGGTTCCTTTTTA CTACAGGATTTCACACAAAGAAAATAGTCCGAGGATCCGCCAGTGATTGGTATGATGCATTGTGTATTCT CCTCCGTCACAGCAAGAATGTGCGGTTTTGGTTTGCTCACAATGTGCTTTTTAATGTTTCAAATCGATTC TCTGAGTACCTTTTGGAATGCCCTAGTGCAGAAGTGAGAGGTGCATTTGCTAAACTTATAGTTTTCATTG CACACTTTTCCTTACAAGATGGGCCTTGTCCTTCACCTTTTGCATCTCCTGGACCTTCTAGTCAGGCTTA TGATAACTTAAGTTTGAGTGACCACCTACTAAGAGCAGTACTAAATCTCTTGAGGAGGGAGGTTTCAGAG CATGGACGTCATTTACAGCAGTATTTCAACTTGTTTGTAATGTATGCCAATTTAGGAGTGGCAGAGAAAA CACAGCTGCTCAAACTGAGTGTACCTGCTACCTTTATGCTTGTGTCCTTAGATGAAGGTCCTGGTCCTCC AATTAAATATCAGTATGCTGAATTAGGCAAATTATACTCCGTAGTGTCACAGTTAATCCGCTGTTGCAAT GTCTCTTCAAGAATGCAGTCTTCAATCAATGGTAATCCTTCTCTTCCAAATCCTTTTGGTGATCCTAATT TATCACAACCTATAATGCCAATTCAACAAAATGTGGTAGACATTTTATTTGTGAGAACAAGTTATGTGAA GAAAATTATTGAAGACTGCAGTAACTCTGATGAGACCGTCAAATTGCTTCGCTTTTGCTGCTGGGAGAAT CCTCAGTTCTCATCTACTGTCCTGAGTGAACTTCTTTGGCAGGTTGCGTATTCCTATACTTACGAACTCC GGCCCTATTTGGATCTGCTTTTACAAATCTTACTGATTGAAGACTCCTGGCAGACTCACAGAATTCATAA TGCCCTTAAAGGAATTCCAGATGACCGAGATGGGCTGTTTGACACAATTCAGCGTTCTAAGAATCACTAT CAAAAAAGAGCATACCAATGTATAAAATGTATGGTAGCCCTCTTTAGCAGTTGTCCTGTGGCTTACCAAA TCCTGCAGGGCAATGGAGATCTTAAAAGGAAATGGACCTGGGCAGTAGAGTGGCTTGGAGATGAACTCGA AAGAAGACCATACACTGGCAATCCTCAGTACACTTACAACAACTGGTCTCCTCCAGTGCAAAGCAATGAA ACATCAAATGGCTACTTCTTGGAGAGATCACATAGCGCTAGGATGACACTTGCAAAAGCTTGTGAACTCT GTCCTGAGGAGGAACCAGATGACCAAGATGCCCCAGATGAGCATGAATCACCTCCACCTGAAGATGCCCC ATTATACCCCCATTCCCCTGGATCTCAATATCAGCAGCCCTATACAGGCCCAGCCGCACATCACATGAAC AACCCTCAGAGAACTGGCCAACGAGCACAAGAAAATTATGAAGGCAGTGAAGAAGTGTCCCCACCTCAGA CCAAGGATCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001135893
Insert Size	7644 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001135893.1, NP_001129365.1
RefSeq Size	10475 bp
RefSeq ORF	7644 bp
Locus ID	363445

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