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Gmcl1 (NM_001010956) Rat Untagged Clone
CAT＃: RN200083
Gmcl1 (untagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 2, (10 ug)
CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Gcl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200083 representing NM_001010956
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCTCTGAGCAGCCGGGTCCTGCGGCCCGCGGGGCGCACAGAGCAGCCCGAACCCACGCCCGGTG CTGGGGGCTCGGCCCGCAGATCGGACGCGGGCGAAGATGCGGCCCACGGCTTCTGTTACTGTCCCGGCGG CCGCAAGCGCAAGCGCAGCAGCGGCACTTTCTGCTACTGTCACCCCGAGTCAGAGACAGACGAAGACGAG GACGAGGGCGACGAGCAGCAGAGGCTGCTGAACACGCCGCGCAGGAAAAAATTAAAGAGCACATCGAAAT ACATCTACCAAACGCTGTTTTTGAATGGTGAAAACAGTGACATTAAGATCTGTGCTCTAGGTGAAGAGTG GAGCTTACACAAAATCTACTTATGTCAATCTGGCTACTTTTCTAGTATGTTCAGTGGTTCTTGGAAAGAA TCCAGCATGAATATTATTGAACTGGAGATTCCTGACCAGAACATTGATATAGAAGCACTGCAGGTTGCAT TTGGATCACTGTATCGAGATGACGTCTTAATAAAGCCCAGCAGGGTTGTCGCCATTTTGGCAGCAGCTTG CATGCTGCAGTTGGATGGTTTGATACAGCAGTGTGGTGAGACCATGAAGGAGACCATCTCTGTAAGAACT GTGTGTGGCTATTACACATCGGCAGGGACCTATGGACTAGACTCTGTAAAGAAAAAGTGCCTCGAGTGGC TGCTGAACAACCTCATGACTCACCAGAGTGTGGAGCTTTTCAAAGAACTCAGTATAAACGTCATGAGGCA GCTCATTGGTTCATCTAACTTATTTGTGATGCAAGTGGAAATGGATGTATATACAGCTCTGAAAAAGTGG ATGTTCCTTCAGTTGGTGCCTTCCTGGAATGGGTCTTTAAAGCAGCTTTTGACAGAAACAGATGTCTGGT TTTCAAAGTGGAAAAAAGACTTTGAAGGGACGACTTTCCTTGAAACTGAGCAGGGAAAACCATTTGCGCC CGTGTTCAGACATTTAAGGCTACAGTACATTATCAGTGATCTGGCTTCTGCAAGGATCATTGAGCAGGAC TCTCTGGTACCTTCAGAATGGCTGGCTTCAGTGTATAAGCAGCAGTGGTTGGCTATGCTTCGGGCTGAAC AAGACAGTGAAGTGGGGCCTCAAGAAATCAATAAAGAAGAACTTGAGGGAAATAGCATGAGGTGTGGTAG AAAGCTTGCCAAAGATGGTGAATACTGCTGGCGGTGGACAGGTTTTAATTTTGGCTTTGACCTCCTTGTG ACTTACACCAATCGATACATCATTTTCAAACGCAATACGCTAAACCAGCCATGTAGTGGATCTGTCAGCT TGCAGCCTCGAAGAAGCATAGCATTTAGATTGCGCTTGGCTTCTTTCGACAGTAGTGGAAAACTCATATG CAGTAGAGCGACTGGCTACCAAATCCTGACGCTTGAGAAAGACCAGGTACGTGTGCTTCTGCGCTTGCGT CTTGTGTGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001010956 |
| Insert Size | 1482 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001010956.3, NP_001010956.1 |
| RefSeq Size | 1952 bp |
| RefSeq ORF | 1482 bp |
| Locus ID | 312516 |
| Gene Summary | This gene encodes a nuclear envelope protein that contains a broad-complex, tramtrack and bric a brac/ Pox virus and Zinc finger (BTB/POZ) protein-protein interaction domain. In mouse, this gene is expressed at high levels in the testis, and null mutant mice exhibit morphologically abnormal sperm that have chromatin condensation defects. These defects result in significantly impaired fertility, demonstrating a role for this gene in male germ cell development in mouse. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] Transcript Variant: This variant (2) lacks the 3'-terminal exon and has a 3' end that extends into an intron compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200083 | Gmcl1 (Myc-DDK-tagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 2, (10 ug) |
CNY 6,370.00 |
|
| RR200083L3 | Lenti ORF clone of Gmcl1 (Myc-DDK-tagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 2, (10 ug) |
CNY 8,270.00 |
|
| RR200083L4 | Lenti ORF clone of Gmcl1 (mGFP-tagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 2, (10 ug) |
CNY 9,030.00 |
