Akap12 (NM_057103) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	AKAP12A; AKAP12B; AKAP12G
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200266 representing NM_057103 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCAGGCAGTTCCACCGAGCAGCGGAGCCCCGAGCAGCCGGCGGGGAGCGACACGCCGAGCGAGC TGGTGCTCAGTGGCCATGGGCCCGCAGCTGAAGCCTCGGGAGCAGCTGGAGACCCCGCCGACGCGGACCC CGCCACCAAGCTCCCACAGAAGAATGGCCAGCTGTCTACTGTCAACGGCGTAGCTGAACAAGGAGATGTC CATGTCCAAGAGGAAAACCAGGAGGGGCAGGAGGAAGAAGTCGTTGATGAGGATGTTGGACAGCGAGAGT CAGAAGATGTGAGAGAAAAAGACCGAGTTGAAGAAATGGCGGCCAACTCCACAGCTGTTGAAGATATCAC AAAGGATGGGCAGGAGGAGACATCAGAAATAATTGAACAGATCCCTGCTTCAGAAAGCAATGTGGAAGAA ATGGTACAGCCTGCTGAGTCCCAGGCTAATGATGTTGGCTTCAAGAAAGTATTTAAATTTGTTGGTTTTA AATTCACGGTGAAGAAGGATAAAAATGAAAAGTCAGATACTGTCCAACTACTCACTGTCAAGAAGGATGA AGGCGAAGGGGCAGAAGCCTCTGTCGGAGCTGGAGACCACCAGGAGCCCAGTGTGGAGACTGCCGTCGGA GAGTCAGCATCCAAAGAAAGTGAGCTGAAGCAATCCACAGAGAAGCAAGAAGGCACCCTGAAGCAAGAAC AGAGCAGCACAGAAATCCCCCTTCAAGCCGAATCTGATCAAGCGGCTGAGGAAGAAGCCAAAGATGAAGG AGAAGAAAAACAAGAGAAAGAGCCCACCAAGTCCCCAGAATCCCCGAGCAGCCCAGTCAGCAGTGAGACA ACATCTTCCTTCAAGAAGTTCTTCACTCACGGTTGGGCCGGCTGGCGCAAGAAGACCAGCTTCAAGAAAT CAAAAGAGGATGATCTGGAAACTGCCGAGAAGAGAAAGGAGCAAGAGGCAGAAAAAGTAGACGAGGAAGA AAAGGAAAAGACAGAGCCAGCCTCGGAGGAGCAGGAGCCGGCAGAAGACACAGACCAGGCCAGGTTGTCA GCAGACTACGAGAAGGTGGAGCTGCCTTTGGAAGACCAGGTTGGTGACCTGGAGGCATCGTCAGAGGAGA AGTGTGCTCCTTTGGCAACGGAAGTGTTTGATGAGAAGATGGAAGCCCACCAAGAAGTTGTTGCAGAGGT CCACGTGAGCACCGTGGAGAAGACAGAGGAGGAGCAGGGAGGAGGAGGAGAGGCTGAAGGGGGCGTGGTG GTAGAAGGAACAGGAGAATCCTTGCCCCCTGAGAAACTGGCTGAGCCCCAGGAGGTCCCCCAGGAAGCTG AGCCTGCTGAGGAGCTGATGAAGAGCAGAGAGATGTGTGTCTCTGGAGGAGACCACACTCAACTGACAGA CCTAAGTCCTGAAGAGAAGACGCTGCCCAAACACCCAGAAGGCATTGTCAGTGAGGTGGAGATGCTGTCC TCTCAGGAAAGAATCAAGGTACAGGGAAGTCCCTTGAAGAAACTCTTCAGTAGCTCAGGCTTAAAGAAGC TGTCTGGGAAGAAGCAGAAGGGGAAACGAGGAGGTGGGGGAGACGAAGAGCCTGGAGAATACCAACACAT TCACACCGAATCCCCAGAGAGTGCTGATGAGCAGAAGGGAGAGAGCTCTGCGTCGTCCCCCGAGGAGCCT GAGGAGACCACGTGTCTGGAGAAAGGGCCGCTGGAAGCACCCCAGGATGGGGAAGCTGAGGAAGGAACTA CTTCCGATGGAGAGAAGAAGAGGGAAGGGATCACTCCCTGGGCATCCTTCAAAAAGATGGTGACACCCAA GAAACGGGTCCGAAGACCTTCTGAGAGTGACAAGGAGGAAGAGCTGGAGAAGGTCAAGAGCGCCACCTTG TCCTCCACTGATAGCACAGTGTCAGAAATGCAAGATGAAGTCAAAACTGTTGGTGAGGAACAAAAGCCAG AGGAACCAAAGCGTAGGGTGGATACTTCAGTGTCTTGGGAAGCACTGATTTGTGTCGGATCATCCAAGAA GAGAGCAAGGAAGGCATCCTCTTCAGATGATGAAGGAGGGCCAAGGACACTGGGAGGGGACAGTCACAGA GCAGAGGAGGCCAGCAAAGACAAAGAAGCCGGAACAGACGCTGTTCCTGCCAGCACCCAGGAGCAGGACC AAGCGCAAGGAAGTTCCTCACCCGAGCCAGCGGGAAGCCCTTCCGAAGGGGAAGGTGTCTCCACTTGGGA GTCATTTAAAAGATTAGTCACTCCAAGAAAAAAATCCAAGTCAAAACTGGAAGAGAAAGCCGAAGACTCT AGTGTAGAGCAGTTGTCCACTGAGATCGAACCGAGTAGAGAAGAATCTTGGGTTTCCATTAAGAAATTCA TCCCCGGACGGCGGAAGAAAAGGGCAGATGGGAAGCAAGAACAAGCCACTGTGGAAGACTCAGGGCCAGT GGAGATAAATGAGGACGACCCTGATGTCCCAGCCGTCGTGCCTCTGTCTGAGTATGATGCAGTGGAGAGG GAGAAGATGGAAGCCCAGGGGAATGCGGAGCTGCCCCAGCTGCTGGGGGCTGTGTACGTGTCCGAGGAGC TCAGTAAGACTCTGGTCCACACTGTGAGTGTCGCAGTCATTGATGGGACCAGGGCAGTCACCAGTGTCGA AGAGCGGTCTCCTTCGTGGATATCCGCTTCCGTAACAGAACCTCTTGAACACACAGCGGGAGAAGCCATG CCACCTGTTGAAGAGGTCACTGAAAAAGACATCATTGCAGAAGAAACTCCTGTGCTCACCCAGACGTTAC CAGAGGGTAAAGATGCCCATGACGACATGGTCACCAGTGAAGTGGATTTCACCTCAGAAGCTGTGACAGC CACAGAGACCTCAGAGGCTCTCCGTACTGAAGAAGTTACCGAAGCATCGGGGGCCGAAGAGACCACAGAC ATGGTGTCCGCAGTTTCCCAGCTGACTGACTCCCCAGACACCACAGAGGAAGCCACCCCAGTTCAGGAGG TAGAGGGTGGTGTGCTAGATACAGAAGAAGAGGAGCGCCAGACGCAGGCCATCCTCCAAGCCGTTGCAGA CAAGGTGAAAGAGGAGTCCCAGGTGCCTGCAACCCAGACTGTGCAGAGAACGGGGTCAAAAGCACTGGAG AAGGTTGAGGAGGTAGAGGAGGACTCCGAAGTGCTGGCTTCGGAGAAAGAGAAGGACGTTATGCCGAAAG GACCCGTGCAGGAAGCTGGAGCTGAGCATCTTGCACAGGGCTCTGAGACTGGACAGGCTACTCCAGAGAG CCTTGAAGTTCCTGAAGTCACAGCAGATGTAGACCATGTCGCCACGTGCCAGGTTATCAAGCTCCAGCAG CTGATGGAACAGGCCGTGGCCCCTGAGTCATCCGAAACCTTGACAGACAGTGAGACAAATGGAAGCACTC CCTTAGCAGATTCAGACACTGCAGATGGGACACAGCAAGATGAAACCATTGACAGCCAGGACAGTAAAGC CACTGCAGCTGTCAGGCAGTCACAGGTCACAGAAGAAGAGGCGGCTACTGCTCAGAAAGAGGAGCCTTCG ACACTACCTAATAATGTTCCAGCCCAGGAAGAACATGGGGAAGAACCAGGAAGAGATGTTCTTGAACCTA CACAGCAAGAGCTTGCTGCTGCAGCCGTGCCCGTTCTGGCAAAGACTGAGGTGGGTCAAGAGGGTGAGGT TGACTGGTTGGATGGAGAAAAAGTCAAAGAAGAACAGGAGGTGTTTGTACACTCTGGACCCAACAGTCAA AAGGCTGCTGATGTGACATATGACAGTGAAGTGATGGGAGTGGCCGGGTGTCAGGAAAAGGAGAGTACTG AAGTGCAGAGTCTTAGCCTGGAGGAGGGAGAGATGGAAACTGACGTTGAAAAGGAGAAAAGGGAGACAAA GCCAGAGCAAGTGAGTGAAGAAGGTGAGCAGGAAACAGCCGCTCCTGAGCATGAAGGAACCTACGGGAAG CCAGTCCTGACACTTGACATGCCCAGCTCAGAGAGGGGGAAGGCACTGGGAAGCCTTGGAGGAAGCCCTT CTCTCCCAGACCAAGACAAAGCAGGTTGCATAGAGGTTCAAGTTCAAAGCCTGGACACAACAGTCACTCA AACAGCAGAAGCTGTGGAAAAGGTCATAGAAACGGTTGTGATTTCAGAGACAGGTGAAAGTCCAGAGTGT GTAGGTGCACACTTATTACCAGCTGAGAAGTCCTCTGCAACGGGTGGCCACTGGACTCTTCAGCATGCAG AGGACACGGTACCCCTGGGGCCTGAGTCTCAGGCAGAATCCATCCCAATCATAGTAACTCCTGCTCCTGA AAGCACCCTACATCCTGACCTACAAGGAGAAATAAGCGCATCCCAGAGAGAGCGATCAGAGGAAGAGGAC AAGCCAGATGCTGGTCCTGATGCTGACGGCAAGGAGAGTACAGCAATCGAAAAAGTCCTCAAGGCTGAAC CTGAGATCCTGGAACTTGAGAGTAAGAGCAACAAGATTGTGCTGAACGTCATTCAGACAGCCGTTGACCA GTTCGCACGTACAGAAACAGCCCCCGAAACTCATGCTTATGATTCACAGACCCAGGTTCCTGCAATGCAG GCTGACAGCCAGGGAGCCCAACAGATGCTGGACAAAAATGAAAGTTGCCAAGATGAAACACCCAGTGCCG CAGCCCAGAGAGGACTTGCAAGTCCTGACCGTTCTGGAGGCATGGGCTCAGCCTCGGAAATGCTTGCCGC GCTTGCAGTTGAAAGCGCCGGTGTCAAAGTAAGCATTGAGAAGCTGCCTCCTCAACCCAAAGATCAAAAG GAGCATGCTGCTGATGGCCCTCAGCTCCAAAGCTTAGCCCAGGCAGAGGCCAGTGCCTCTGGAAACCTAA CCAAAGAATCCCCAGACACCAACGGACCAAAGCTAACCGAGGAGGGCGATGCCCCAAAAGTTGAGGTCCA GGAAGAAGAAATGTCTACCAAGTCAGTCAAAGAGAACAAGGCCCAGGCAGAAGAGGACCTGCAGGAGCCA AAGGGAGACCTGGCAGAATCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_057103
Insert Size	5064 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_057103.2, NP_476444.2
RefSeq Size	6255 bp
RefSeq ORF	5064 bp
Locus ID	83425
UniProt ID	Q5QD51
Gene Summary	Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).[UniProtKB/Swiss-Prot Function]

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