Lig1 (NM_030855) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MGC105416
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200285 representing NM_030855 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGAAGTATTATGTCATTTTTCCAACCCACAACAACAGAAGGTAAAGCGAAGAAGCCAGAGAAGG AGATACCCAGCAGCATCAGAGAGAAGGAACCCCCTCCAAAGGTGGCATTGAAGGAGAGGAATCGAGCAGT GCCTGAGAGTGATTCTCCAGTGAAGAGGCCAGGAAGGAAGGTGGCCCAGGTTCTGAGCAGTGAAGGGGAG GACGAAGATGAAGCCCCTGGCACCCCCCAAGTCCAAAAGCCTGTGTCAGACTCCAAACAGAGCTCTCCTC CCAGCCCTGACTCCTGTCCTGAGAACAGTCCTGTCTTCAACTGCAGCCCCTCCATGGACATCTCCCCATC AGGATTCCCAAAGCGCCGAACTGCACGGAAGCAGCTCCCAAAACGGACAATTCAAGACACTCTTGAGGAG CCCAATGAAGACAAAGCCAAAGCAGTTAAGAAAAGGAAAAAAGAAGATCCCCAGACCCCACCAGAAAGCC TCACAGAGGCTGAAGAAGTAAACCAGAAGGAAGAACAGGTGGAGGACCAGCCCACAGTACCCCCTGAGCC CACAGAGTCCCCTGAGTCAGTAACCCTCACAAAGACAGAGAACATTCCAATGTGTAAGGCAGGTGTGAAA CAGAAGCCTCAGGAAGAAGAACAGAGCAAGCCTCCTGCCAGAGGTGCCAAGCCACTGAGCAGTTTCTTCA CACCCCGAAAACCAGCAGTGAAAACTGAAGTCAAACAAGAAGAGTCAGACACTCCAAGGAAGGAAGAGAC CAAGGGAGCCCCGGATCCAACCAATTATAATCCTTCCAAGAGTAACTACCATCCCATTGAAGATGCCTGC TGGAAACATGGCCAAAAGGTTCCTTTTCTTGCTGTGGCCCGGACATTTGAGAAGATTGAGGAGGTGTCTG CTCGGCTTAAGATGGTGGAGACACTGAGCAACCTGCTGCGCTCCGTGGTGGCCCTGTCCCCAACAGACCT GCTCCCTGTTCTCTATCTCAGCCTCAACCGCCTTGGGCCGCCTCAGCAGGGTCTAGAACTGGGCGTTGGT GATGGTGTCCTCCTCAAGGCAGTTGCCCAGGCCACAGGTCGTCAGCTGGAGTCCATCCGGGCTGAGGTAG CTGAGAAGGGGGATGTGGGGCTAGTGGCGGAGAACAGCCGAAGCACTCAGAGGCTCATGCTGCCTTCACC TCCACTTACTGTTTCTGGAGTCTTTACCAAATTCTGTGACATTGCCCGGCTCACTGGCAGTGCTTCCATG GCCAAGAAAATGGACATTATCAAGGGCCTTTTTGTTGCCTGCCGCTACTCAGAAGCCCGGTTTATTGCCA GGTCCCTAAGTGGGCGGCTGCGCCTTGGGCTGGCTGAGCAGTCAGTACTGGCTGCTCTTGCCCAGGCTGG GAGCCTCACACCCCCTGGCCAAGAATTTCCCACGGTGGTGGTGGATGCTGGGAAGGGCAAGACAGCAGAG GCCAGAAAGATGTGGTTGGAAGAACAAGGCATGATCTTGAAGCAAACCTTCTGTGAGGTGCCTGATCTGG ATCGCATCATCCCTGTGCTGCTGGAACATGGCTTGGAAAGTCTCCCAGAGCACTGCAAGCTGAGCCCAGG GGTTCCTCTTAAACCAATGCTGGCCCATCCCACTCGGGGTGTCCGTGAGGTCCTGAAACGCTTTGAGGAG GTCGACTTCACCTGCGAGTATAAATACTATGGGCAGCGGGCACAGATACATGTTCTGGAAGGTGGAGAAG TGAAGATCTTTAGCAGGAACCAGGAAGACAACTCAGGAAAGTATCCTGACATCATCAGCCGCATCCCCAA GATTAAACACCCCTCAGTCACATCTTTCATTCTGGACACTGAGGCTGTGGCCTGGGACCGGGAAAAGAAG CAGATTCAGCCATTCCAAGTGCTCACCACACGCAAGCGCAAGGAGGTTGATGCATCGGAGATACAGGTAC AAGTGTGTCTGTATGCTTTTGACCTCATCTACCTCAACGGAGAGTCCCTGGCTCGCCAGCCCCTGTCTCG ACGCCGGCAGCTACTCCGTGAGAACTTTGTGGAGACAGAGGGTGAGTTTGTCTTTGCCACCTCCTTGGAC ACCAAGGACATCGAGCAGATTGCCGAGTTCTTAGAGCAGTCAGTGAAGGACTCCTGTGAGGGCCTGATGG TGAAGACCCTGGATGTTGATGCTACCTATGAGATCGCCAAGAGGTCACACAACTGGCTCAAGCTGAAGAA GGACTACCTTGAAGGTGTGGGCGACACTCTAGACCTTGTGGTGATTGGTGCCTACCTGGGCCGGGGGAAG CGGCCTGGCCGGTATGGGGGCTTCCTGTTGGCTGCCTACGATGAGGAGAGTGAAGAGCTTGCAGCCATAT GCAAGCTGGGAACCGGATTCAGCGACGAGGAGCTGGAAGAACATCATCAGAACATGCAGGCCCTTTTATT GCCTACCCCACGCCCCTATGTGAGGATTGATGGGGCAGTGGCCCCAAACCACTGGCTGGACCCAAGCATT GTGTGGGAGGTGAAGTGTGCTGATCTTACCCTGTCTCCCATCTACCGTGCTGCACGGGGCATGGTGGACA AAGAAAAAGGGATCTCCCTTCGTTTCCCTCGGTTCATTCGTGTCCGTGAGGATAAGCAGCCAGAGCAGGC CACTACCAGTGACCAGGTGGCCTCTTTGTACCGGAAGCAGAGTCAGATTCAGAACCAGCAAAGCTCAGAC TTGGACTCTGATGTTGAAGATTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_030855
Insert Size	2757 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_030855.1, NP_110482.1
RefSeq Size	3112 bp
RefSeq ORF	2757 bp
Locus ID	81513
Gene Summary	catalyzes the joining of blunt ended DNA [RGD, Feb 2006]

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