Plcg2 (NM_017168) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200573 representing NM_017168 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACCATGGTCAACGTGGACACCCTTCCAGAATATGAGAAGAGTCAGATCAAGAGAGCCCTGGAGC TAGGGACGGTGATGACCGTATTCAGTGCCCGCAAATCCACCCCAGAGCGGAGGACAGTACAGATGATCAT GGAGACCCGGCAGGTGGCATGGAGCAAGACAGCAGATAAGATCGAAGGCTTCTTGGACATCATGGAAATA AAGGAAATCCGGCCAGGGAAGAACTCCAAGGACTTTGAGCGAGCCAAGGCTGTCCGTCACAAGGCAGACT GCTGCTTCACCATCTTCTACGGCACCCAGTTCGTGCTCAGCACTCTCAGTCTGGCAACCGACTCAAAGGA GGATGCAGTGAAGTGGCTCTCTGGCTTGAAGATCTTACACCAGGAAGCGATGAACGCATCCACTCCCACA ATGATTGAGAGTTGGCTGCGGAAACAGATTTATTCAGTAGATCAAACCCGAAGAAACAGCATCAGCCTCC GGGAGCTGAAGACCATCTTGCCCCTGGTCAACTTCAAAGTGAGCGGCATCAAGTTCCTCAAGGACAAGCT GGTGGAAATTGGCGCCCAGAAAGATGAGCTCAGTTTTGAACAGTTCCATCTCTTCTATAAAAAACTCATG TTTGAGCAGCAGAAGTCGATACTTGACGAATTCAAAAAAGACTCCTCCGTGTTCATCCTAGGGAACACAG ACCGGCCTGATGCCTCAGCCGTCTACCTGCAAGACTTCCAGAGGTTTCTTTTACATGAACAGCAGGAGCT ATGGGCTCAGGATCTGAACAAAGTCCGGGAGCGGATGACCAAGTTTATCGATGACACAATGAGGGAGACC GCAGAGCCCTTCTTGTTTGTGGATGAGTTCCTCACGTATCTGTTCTCACGGGAGAACAGCATCTGGGACG AGAAGTACGATGCCGTGGACATGCAGGACATGAACAACCCCTTGTCCCATTACTGGATCTCCTCTTCCCA CAACACGTACCTCACTGGAGACCAGCTGCGTAGCGAGTCCTCCACGGAAGCGTATATCCGCTGTCTGCGT GCTGGTTGTCGCTGCATCGAGTTGGATTGCTGGGATGGGCCTGACGGGAAACCCATAATCTATCACGGTT GGACACGGACCACCAAGATCAAGTTTGATGATGTTGTTCAGGCCATCCGGGATCATGCCTTTGTTACCTC CAGCTTTCCCGTGATTCTGTCTATCGAGGAGCACTGCAGTGTGGAGCAGCAACGTCACATGGCCAAGGTC TTCAAGGAAGTGTTAGGAGACCTGCTGTTGACGAAGCCCACGGAGGCCAGTGCTGACCAGCTGCCCTCAC CCAGCCAGCTTCGTGAGAAGATCATTATCAAGCATAAGAAGCTGGGCCCCCGAGGTGATGTCGATGTCAA TGTGGAGGACAAGAAAGATGAGCACAAGACCCAGGGCGAGCTGTATATGTGGGACTCCATCGACCAGAAA TGGACTCGCCACTACTGTGCTATTGCGGACGCCAAGCTGTCCTTCAGTGACGACATTGAACAGACTGTGG AAGAGGATCCGGTCCAGGACACTCCCCCCACGGAGCTACATTTTGGGGAGAAATGGTTCCATAAGAAGGT GGAGAGCAGGACCAGTGCGGAGAAGCTGCTGCAGGAGTACTGTGCCGAGACTGGGGCCAAGGATGGCACC TTCCTGGTGCGGGAGAGCGAGACCTTCCCCAACGACTACACACTCTCCTTCTGGCGGTCTGGCCGGGTGC AGCACTGCCGAATCCGGTCTACCATGGAGGGTGGGGTCATGAAGTACTACCTGACTGACAACCTCACGTT CAACAGCATCTACGCCCTCATCCAGCACTACCGGGAGGCACACCTGCGCTGTGCGGAGTTCGAGCTGCGG CTCACAGACCCGGTGCCCAACCCCAACCCACATGAGTCCAAGCCGTGGTACTATGACAGGCTGAGCAGGG GGGAAGCAGAGGACATGTTGATGCGGATTCCCCGAGATGGAGCCTTCCTCATTCGGAAGCGGGAGGGGAC CGACTCTTACGCCATCACCTTCAGGGCCAGGGGCAAGGTGAAACATTGCCGTATCAACCGGGACGGACGT CACTTTGTGCTGGGGACCTCTGCCTACTTTGAGAGCCTGGTGGAGCTCGTCAGCTACTATGAGAAGCACG CACTCTACCGCAAGATGAGGCTTCGCTACCCCGTGACCCCGGAGCTCCTGGAACGATATAATATGGAAAG AGACATCAACTCTCTCTATGACGTCAGCAGGATGTATGTGGACCCAAGTGAGATCAACCCTTCCATGCCT CAGAGGACTGTGAAAGCACTCTATGACTACAAAGCCAAGCGCAGCGATGAGCTGACCTTCTGCCGAGGTG CCCTCATCCACAACGTCTCCAAGGAGCCGGGAGGATGGTGGAAAGGGGACTATGGGACCCGGATCCAGCA GTATTTCCCATCTAACTACGTTGAAGATATCTCGGCAGGCGATGCTGAGGAGATGGAAAAGCAGATTATT GAAGACAACCCCCTCGGGTCTCTCTGCAGAGGCATATTGGATCTTAATACCTACAATGTGGTGAAGGCCC CCCAGGGGAAAAACCAGAAAGCCTTCGTCTTTATCCTGGAGCCTAAGAAACAGGGGGACCCTCCTGTGGA GTTCGCGACTGACCGTGTGGAGGAACTGTTTGAGTGGTTTCAGAGTATACGAGAGATCACTTGGAAGATC GACACCAAGGAGAACAACATGAAGTACTGGGAGAGGAATCAGTCCATTGCTATTGAGCTCTCTGACTTGG TTGTGTACTGCAAGCCGACTAGCAAAACCAAGGACCATTTGGAAAATCCTGACTTCCGGGAAATTCGCTC TTTTGTGGAGACAAAGGCAGACAGCATTGTCAGGCAAAAGCCCGTGGATCTATTGCGGTACAACCAGAAG GGCCTGACCCGTGTCTACCCCAAGGGACAGAGAGTTGACTCTTCCAACTACGACCCCTTCCGTCTGTGGC TGTGTGGCTCCCAGATGGTGGCGCTCAATTTCCAGACTCCAGACAAGTACATGCAGATGAACCATGCATT GTTTTCGCTCAATGGGCGGACAGGTTACGTCCTGCAGCCCGAGAGCATGCGGTCTGAGAAGTATGACCCG ATGCCCCCGGAGTCCCAGAGGAAGATCCTGATGACACTCACTGTCAAGGTTCTTGGTGCACGCCACCTCC CTAAACTAGGGCGGAGTATCGCCTGTCCCTTTGTAGAGGTGGAAATCTGTGGGGCCGAGTATGACAGCAA CAAATTCAAGACGACGGTTGTGAATGACAACGGTCTCAGCCCTGTCTGGGCTCCAACTCAGGAGAAGGTG ACATTTGAAATTTATGACCCAAACCTTGCGTTCCTACGCTTTCTGGTCTACGAAGAAGACATGTTCAGTG ACCCCAACTTCCTGGCTCATGCCACATACCCCATTAAAGGCATCAAATCAGGATTTAGATCAGTCCCTCT GAAGAACGGGTACAGTGAAGACATCGAGCTGGCATCCCTCCTGGTTTTCTGTGAGATGCGGCCAGTCCTG GAGAGTGAAGAAGAACTCTATTCCTCCTGTCGCCAGCTGCGGAGGCGGCAGGAAGAGCTCAACAACCAGC TCTTCCTGTATGACACACACCAGAACCTGCGAGGAGCCAACCGGGATGCCCTGGTGAAGGAGTTCAATGT TAACGAGAATCAGCTGCGGCTGTACCAGGAGAAGTGTAACCGGAGGCTGAGAGAGAAGAGAGTGAGTAAC AGCAGGTTCTACTCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017168
Insert Size	3798 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017168.1, NP_058864.1
RefSeq Size	4321 bp
RefSeq ORF	3798 bp
Locus ID	29337
UniProt ID	P24135
Gene Summary	has phosphoinositide-specific phospholipase C activity [RGD, Feb 2006]

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