Il16 (NM_001105749) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200610 representing NM_001105749 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCCACAGCCACTCTGGAAAGAGCCGGAAATCCACGAAATTTCGCTCCATTTCCCGTTCCCTGA TCCTCTGTAACGCCAAGACCAGTGATGACAGCTCCAGCCCTGATGAGAAGTACCCCGACCCTTTTGAGAC TTCACTGTGCCAGGACAAGGAGGGCTTCTTTCACTCCTCCATGCAGCTGGCAGACACATCTGAGGCTGGG CTCAGCAACATACCAGACCTGGCACTGGCCTCAGAGGCTGCTCAACTCCAAGCAGCTGGGAGCGATCGGG GCAAGCACTGCAGGAAGATGTTCTTCCTGAAGGAATCTTCTACAACTTCCTCTAAAGAAAAGGCTGGAAA ATCAGAAGCACAAAGTAGCAACTTCCTGTTTCCTAAAGCCTGCCACCAAAGGACACGCAGCAACTCCACC AGTGTTAATCCCTACAGTGCCAGAGAAATGGATTTTCCAATGACCAAGAAATCTGCAGCTCCCACCGACA GGCAGCCCTACTCTCTCTGTAGCAACAGGAAGTCCCTCTCTCAACAACTGGACTACCCAGTTTCGGGCAC TGCAAGACCAACTCGGTCACTGAGCACAGCTCAGCTCGGGCAGCTGTCTGGGGGTCTGCAGGCGTCAGTC ATTTCCAACATAGTGCTGATGAAGGGCCAGGCAAAGGGCCTGGGCTTCAGCATTGTTGGGGGAAAGGACA GTATTTACGGCCCTATTGGGATTTACGTGAAGAGCATCTTTGCAGGGGGAGCAGCTGCAGCCGACGGGCG GCTTCAGGAAGGTGATGAAATTCTGGAACTCAATGGTGAATCAATAGCTGGCCTAACACACCAGGATGCT TTGCAGAAGTTTAAGCAAGCCAAGAAGGGACTGCTGACCCTGACAGTGAGGACGCGCCTGACAACACCCC CTTCACTATGCAGCCACCTGTCTCCCCCGCTGTGCCGCTCCCTGAGCTCCAGCACGTGCGGTGCCCAGGA CAGCAGCCCCTTCTCCTTGGAAAGCCCAGCGTCTCCTGCCAGCACTGCCAAACCCAACTACAGAATCATG GTGGAGGTGTCACTGAAGAAAGAGGCAGGTGTTGGCCTGGGCATTGGCCTGTGCAGCATCCCCTACTTCC AGTGCATTTCGGGCGTCTTCATTCACACGCTGTCACCAGGATCCGTGGCGCATCTCGATGGACGGCTGAG GTGTGGTGATGAGATTGTGGAAATCAATGATTCTCCAGTGCACTGCATGACCCTCAATGAAGTCTACACA ATCCTGAGCCACTGTGACCCTGGCCCAGTTCCCATCATCGTTAGCCGACATCCGGACCCACAGGTGTCTG AACAACAACTCAAGGAAGCTGTGGCTCAGGCAGTGGAAGGCGTCAAGTTTGGGAAGGAAAGGCACCAGTG GAGCTTGGAAGGTGTCAAAAGGCTGGAGAGCAGTTGGCATGGGCGGCCCACCTTGGAGAAAGAACGTGAG AAGCACTCAGCGCCCCCGCATCGAAGGGCCCAGAAGATCATGGTCCGATCCAGCAGTGACAGCAGCTACA TGTCTGGGTCACCAGGGGGAAGTCCCTGCAGTGCAGGTGCGGAGCTACAGCCTTCTGAGGGGGAAGGCAG CACACACAGCCCCAGCCTGCCCCCAGGGGAGGAGCAAGAGCCGTGTCCAGGGGTCCCATCCAGGCCACAT CAGGAGAGCCCACCCCTTTCTGAGAGCTTGGAAAGAGACAGCCACCCACCACTGAAACTGAAGAACTCTT TTGAGATTTTGGTGAGAAAGCCGACATCCACCAAACCCAAGCCTCCACCCAGAAAATACTTTAAAAATGA CAGCGAGCCTCAGAAGAAACTGGAGGAGAAAGAGAAGGTCACAGACCCTTCTGGGCACACCTTGCCCACC TGCAGCCAGGAAACCAGAGAGCTGCTGCCACTGCTGCTACAGGAAGACACTGCAGGGAGAACCCTCTGCA CCGCTGCTTGCTGCCCAGGACCTGCAGCCAGCACGCAGACTTCATCCTCCACAGAAGGAGAGCCAAGGAG GAGTGCCAGCCCAGAGACTCCAGCATCCCCAGGAAAACACCCGCTGCTGAAGAGGCAGGCGCGGATGGAC TATAGCTTTGACAGCACCACTGAAGACCCTTGGGTTAGAATTTCCGACTGCATCAAAAACCTATTTAGCC CTATCATGAGTGAGAACCATAGCCATATGCCACTACAGCCCAATGCCAGCCTTGGTGAAGAGGACGGGAC ACAGGGCCACCCAGAGGGGGGCCTGTCAAAAATGGACACTGCCAATGGTGCTCCCAGGATTTTCAAGTCA GCAGATGGCAGCACTGTGAAGAAGGGACCCCCAGTAGCCCCAAAGCCAGCCTGGTTTCGGCAGAGCTTGA AAGGTCTGAGGAATCGTGTTCCAGAAACCAGAAGAGCCCCGGAAGTAGCTTCAGCCATTCAGACAACACC AGTTTCTAGGGATGCCTCAGGGCCACAGTCTCAGGCCTCCTCCTCCTCCATCCGGCAGAGAATCAGCTCC TTTGAAAACTTTGGCTCCTCACAATTGCCAGATAGAGGAGTCCAGAGACTGAGCCTGCAGCCCACCTCTG GGGAGACTACCAGACTTCCAGGAAAGCAAGATGAAGGACGGTTTTCTGGTCTCCCGGCTCAAGGGACTAA AGTAACTGCTAAACACAGGCAGACAGAGGTAGAGCCTCCATCTACAACCTTCCCTAACTCCTCAGAGGTC GGGGACCCAGATATGTCTGAGTCTCCTCCCCCAGGTCAGCGACCCAACCCGAAAGCTTTGTCTCCAGACC CTCTCTTGAGACTGTTGACTACACAAACTGAGGATACTCAAGGACCAGGTTTCAAGATGCCAAGTCAGCG GGCACGGAGCTTCCCCCTGACCAGGACCCAGTCCTACGAGACAAAGCTGCTGGATGAAAAGTCCAGCAAG CTCTACTCCATTAGCAGTCAGCTATCCTCTGCTGTCATGAAATCCCTGCTGTGCCTTCCATCTTCAGCCT CTTGTGGCCAGATCACGTGCATCCCCAAGGAGAGGGTGTCTCCAAAGTCACCCTGCAACAACAGCGCAGC TGCAGAGGGTTTTGGTGAAGCTGTGGCCTCCGACACAGGGTTTTCTCTCAACCTTTCAGAGCTGAGAGAA TATTCAGAAGGTCTCACAGAACCCGGGGAAACCGAGGACAGGAACCACTGTTCTTCTCAGGCCGGTCAGT CAGTTATCTCCTTGCTGAGTGCAGAAGAACTGAAAAAACTCATTGAGGAAGTGAGAGTTCTAGACGAGGC GACACTGAAGCAATTAGACAGCATTCACGTCACCGTCTTACACAAGGAAGAAGGTGCTGGGCTTGGATTC AGCTTGGCAGGAGGGGCAGATCTAGAAAACAAGGTGATCACGGTTCACAGAGTGTTTCCAAATGGACTGG CTTCCCAGGAAGGCACGATTCAGAAAGGCAATGAGGTTCTTTCCATCAATGGCAAATCTCTCAAGGGCGC TACCCACAATGATGCCCTAGCTATCCTTCGCCAAGCTCGGGACCCAAGGCAAGCTGTGATTGTCACCAGG AAAGCAATTGTGGAGGCCGCTCATGACCTGAACTCCTCTACTGACTCTGCAGCATCAGCTTCAGTAGCCA GTGGCATTTCTGTAGAATCTGTGGAGGCCACTGTCTGCACAGTGACTCTGGAGAAGACCTCTGCAGGGCT GGGCTTCAGCCTGGAAGGAGGAAAGGGCTCCCTGCATGGTGACAAGCCTCTTACCATAAACAGGATTTTC AAAGGAACAGAACAAGGTGATGCGGTCCAGCCAGGGGATGAGATCTTGCAGCTCGCTGGCACTGCTGTGC AAGGTCTTACACGGTTTGAAGCCTGGAATGTCATCAAGGCATTACCTGATGGACCTGTCACTGTAGTCAT CAGAAGGAACGGCCTCGAGGGCAAGCAGACGACAGCTTCTGCAGACTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001105749
Insert Size	3972 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001105749.1, NP_001099219.1
RefSeq Size	4985 bp
RefSeq ORF	3972 bp
Locus ID	116996

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