Abcc12 (NM_199377) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200649 representing NM_199377 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGCGAAGACCCGTACCTTATCTCAGACCTGGATCGCAGAGGCCGTCGGAGATCCTTTGCCGAGA GATATGATCCCAGCCTGAAAACCATGATCCCAATGCGACCCTATGCAAGGCTGGCACCCAATCCTGTGGA TGATGCGGGGCTGCTGTCCTTCGCCACGTTTTCCTGGCTCACACCTGTGATGATTCGAAGTTACAAGCAC ACGCTGACCGTGGACACCCTGCCTCCACTATCTCCTTACGACTCATCGGATGTCAATGCCAAGAGACTCC AAATCCTTTGGGATGAAGAAATAGAAAGGGTAGGGCCCGAGCGGGCCTCTTTGGGCCGTGTGGTTTGGAA ATTCCAGAGGACCCGTGTTCTGATGGATGTTGTAGCCAACATTCTCTGCATCATCATGGCAGCCTTAGGG CCGACAGTTCTCATTCACCAAATCCTCCAGCACGTTACCAACATCTCCTCCGGACACATCGGGATCAGCA TCTGCTTGTGTCTGGCCCTTTTTGCCACCGAGTTTACCAAAGTCCTCTTTCGGGCCCTTGCCTGGGCCAT AAATTACCGCACAGCGATTCGACTGAAGGTGGCCCTGTCCACGCTAATTTTCAAAAATCTGTTGTCCTTC AAGACATTAACCCACATCTCTGCAGGCGAGGTACTCAATGTACTATCGAGTGATAGTTATTCCTTGTTTG AGGCTGCCTTGTTTTGTCCTTTGCCAGCCACCATCCCGATCCTAATGGTCGTTTGTGCCGTGTATGCCTT TTTCATTCTGGGGTCCACCGCTCTTGTTGGGATATGCGTGTATCTCATATTCATCCCGATCCAGATGTTT ATGGCCAAACTCAACTCAGCTTTCCGAAGATCAGCAATTTCAGTGACAGACAAGCGGGTTCAGACAATGA ATGAGTTTCTGACCTGCATCAAACTGATTAAAATGTATGCCTGGGAGAAATCTTTTATGAACACCATTCA CGACATAAGAAAGAGAGAGAAAAAATTACTGGAAAAGGCTGGATACGTCCAAAGTGGGAACTCAGCCCTG GCTCCCATAGTGTCTACCATAGCCATTGTGTCTACTTTCACCTGCCACATTTTCCTGAAACGCACACTCA CTGCCCCTGTGGCATTTAGTGTGATTGCCATGTTTAATGTAATGAAGTTTTCAATTGCAATCTTGCCTTT CTCGGTCAAAGCAGTGGCCGAAGCCAGTGTCTCTCTAAGGAGAATGAAGAAAATTCTTGTAGCTAAAAGT CCCCCATCCTACATCACCCAACCAGAAGACCCAGATACTATCTTGCTTTTAGCAAATGCCACCCTGACAT GGGAACAAGAAATCAACAGGAAAAGAGGCCCATCGAAGACACAAGATCAGAGAAGGCACGTTTTCAAGAA ACAGAGGGCAGAGCTATACAGTGAGCAAAGTCTCTCAGATCAGGGAGTTGCCAGCCCAGAGCGGCAAAGT GGCAGCCCCAAGTCAGTACTACACAACATCAGCTTTGTGGTGAGAAAGGGAAAGGTCTTGGGGATATGCG GGAATGTTGGAAGCGGGAAGAGTTCCCTCATTTCTGCTCTTCTAGGGCAGATGCAGTTACAGAAAGGGGT CGTGGCTGCCAGTGGACCTTTGGCTTATGTTTCTCAGCAAGCTTGGATCTTCCACGGAAATGTGAGGGAG AACATCCTGTTTGGAGAGAAGTACAACCACCAAAGGTACCAACACACGGTTCATGTCTGTGGGCTCCAGA AGGATTTGAACAGCCTCCCTTATGGAGACCTGACTGAGATTGGGGAGCGGGGTGTGAACCTCTCCGGGGG ACAGAGGCAGAGGATCAGCCTGGCCCGGGCTGTGTACGCCAACCGTCAGCTCTACCTGCTGGATGACCCG CTCTCGGCAGTGGATGCCCATGTGGGGAAGCACGTCTTTGAAGAATGCATTAAAAAGACACTCAAGGGGA AGACTGTGGTGCTGGTTACCCACCAGCTGCAGTTCCTGGAGTCTTGCGATGAGGTCATTCTGTTAGAAGA TGGAGAGATCTGTGAAAAGGGCACCCACAAGGAGTTGATGGAAGAGAGAGGCCGCTATGCAAAGCTGATC CACAACCTCAGGGGACTGCAATTCAAGGATCCAGAGCACATTTACAATGTAGCCATGGTGGAGACCCTGA AGGAGAGCCAAGCTCAAAGGGATGAAGACGCTGTCTTGGCTTCAGGAGATGAGAGAGATGAAGGAAAAGA ACCCGAGACAGAAGAATTTGTGGACATAAAAGCTCCCGTGCACCAGCTCATCCAGATTGAATCCCCCCAA GAAGGAATCGTGACCTGGAAAACATATCACACGTATATTAAAGCTTCTGGAGGGTACCTGGTCTCCTTCC TGGTTTTGTGTCTCTTCTTCCTGATGATGGGCAGCTCTGCCTTCAGCACCTGGTGGCTGGGGCTCTGGTT GGACAGTGGCTCCCAGGTCATCTGTGCACCCCAGAGCAATGAGACAGCCTGCAACGTTAACCAGACGCTG CAGGACACCAAACACCACATGTACCAGTTGGTTTACATAGCAAGCATGATGTCTGTGCTGACGTTTGGCA TCATCAAAGGCTTCACCTTCACCAACACGACACTGATGGCGTCCTCCTCCCTCCACAACAGAGTGTTTAA CAAGATCGTCAGCAGCCCAATGAGCTTCTTTGACACGACTCCCACAGGCCGGCTAATGAACCGCTTTTCT AAAGATATGGATGAGCTAGATGTGAGGCTGCCATTTCACGCTGAGAATTTTTTGCAGCAGTTTTCTATGG TGGTGTTTATTCTGGTGATTATGGCTGCCTCGTTTCCTGTTGTCCTCGTGGTGCTGGCTGGCCTGGCTAT TCTCTTCTTCATCCTTTTACGCATTTTCCATCGAGGGGTGCAGGAGCTCAAGCAGGTGGAGAACATCAGC CGGTCCCCTTGGTTCTCTCACATCACCTCCTCCATGCAGGGTCTGGGTGTCATCCATGCCTACGACAAAA AGGACGATTGCATCAGCAAGTTTAAGGCGCTCAATGATGAAAACTCCAGCCACCTCTTGTACTTTAACTG TGCACTCAGGTGGTTTGCTCTCAGAATGGACATCCTCATGAACATCGTCACCTTTGTCGTGGCCTTGCTG GTGACCCTGAGCTTTTCTTCAATCAGTGCTTCATCCAAAGGCTTGTCCCTGTCTTACATCATCCAGCTCA GTGGACTGCTTCAAGTGTGTGTGCGAACAGGAACAGAGACCCAAGCCAAGTTCACCTCTGCAGAGCTAAT GAGGGAGTACATTTCGACCTGTGTCCCTGAACACACTCAATCCTTCAAAGTGGGGACCTGTCCCAAAGAC TGGCCGAGCCGTGGGGAGATAACTTTCAAGGACTATCGGATGAGATACAGAGACAACACCCCTCTCGTTC TCGATGGGTTGAACCTGAACATCCAAAGCGGGCAGACAGTTGGGATTGTGGGAAGAACGGGCTCTGGTAA ATCATCACTGGGCATGGCCCTGTTTCGTCTGGTGGAACCAGCCTCTGGCACCATCTTCATTGACGAGGTG GATATCTGCACTGTGGGTCTGGAAGAGCTCCGAACCAAGCTGACCATGATCCCCCAGGATCCTGTCCTGT TTGTAGGTACAGTAAGGTACAACTTGGATCCCTTGGGGAGTCACACCGACGAGATGCTCTGGCATGTTTT GGAGAGAACATTCATGAGAGACACAATAATGAAACTCCCAGAGAAATTACAGGCAGAAGTCACAGAAAAC GGGGAAAACTTCTCAGTAGGAGAACGCCAGCTGCTTTGTATGGCCCGGGCACTTCTCCGTAATTCAAAAA TCATTCTCCTTGATGAAGCTACTGCCTCCATGGATTCCAAGACGGACACCCTTGTTCAGAGCACCATAAA AGAGGCCTTCAAAAGCTGCACAGTGCTGACCATCGCTCATCGCCTAAACACTGTTCTCAACTGTGACCTT GTCCTGGTCATGGAAAATGGGAAGGTGATTGAGTTTGACAAGCCTGAAGTCCTCGCTGAGAAGCCTGACT CTGCATTTGCGATGTTACTAGCTGCAGAAGTTGGACTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_199377
Insert Size	4101 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_199377.1, NP_955409.1
RefSeq Size	4541 bp
RefSeq ORF	4101 bp
Locus ID	291923
UniProt ID	Q6Y306
Gene Summary	member of the ATP-binding cassette (ABC) transporter family; human homolog is a candidate disease gene for paroxysmal kinesigenic choreoathetosis [RGD, Feb 2006]

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