Abcc1 (NM_022281) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Abcc1a; Avcc1a; Mrp; Mrp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200856 representing NM_022281 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGAGCAGCTTCTGCAGCTCTGATGGCTCCGATCCGCTCTGGGATTGGAATGTCACATGGCACA CCAGCAACCCTGACTTTACCAAGTGCTTTCAGAATACGGTCCTCACATGGGTGCCTTGTTTCTACCTCTG GTCCTGTTTCCCCCTCTACTTCCTCTATCTCTCTCGACATGACCGGGGCTACATCCAGATGACACACCTC AACAAAGCCAAAACTGCCTTAGGATTCTTTCTGTGGATCATCTGCTGGGCAGACCTCTTCTACTCTTTCT GGGAAAGAAGTCAGGGAATGCTCCTAGCCCCGGTGCTACTGGTCAGCCCGACACTGCTAGGCATCACCAT GCTGCTCGCCACCTTTTTAATTCAGTTTGAGCGAAGGAAAGGAGTCCAGTCCTCAGGGATAATGCTTACT TTCTGGCTTGTAGCCCTACTCTGCGCCCTTGCCATCTTGAGATCTAAGATCATCTCTGCCTTAAAAAAGG ATGCTCAAGTGGACATGTTTCGAGATTCTGCATTCTATCTCTACTTCACCCTCGTGTTCATTCAGCTTGT GCTGTCCTGCTTCTCAGACAGCTCACCCTTGTTCTCTGAAACTGTCCGTGACCCGAATCCATGTCCAGAA TCGAGTGCCTCTTTTCTTTCCAGGATCACTTTTTGGTGGATTACAGGGATGATGGTGCAGGGCTACCGCC AGCCCCTGAAGAGCAGTGACCTCTGGTCATTGAATAAAGAGGACACGTCAGAAGAAGTGGTACCTGTGCT GGTGAATAACTGGAAGAAGGAATGTGTTAAGTCGAGGAAGCAGCCTGTACGGATTGTGTATGCCCCTCCC AAAGATCCCACCAAGCCTAAGGGAAGTTCTCAGTTGGATGTGAATGAGGAAGTGGAGGCACTGATTGTCA AGTCATCCCACAAGGACCGGGACCCCTCTCTGTTCAAGGTGTTGTACAAGACCTTTGGGCCCTACTTCCT CATGAGCTTCCTGTACAAGGCCCTTCATGACCTGATGATGTTTGCTGGCCCTGAGATCTTGGAATTGATT ATCAACTTCGTGAATGACAGGGAGGCCCCTGACTGGCAGGGCTACTTGTACACAGCACTGCTGTTTGTCA GTGCCTGTCTGCAGACACTGGCACTCCACCAGTACTTTCATATCTGCTTCGTCACCGGCATGCGCATCAA GACTGCTGTGGTGGGTGCTGTTTACCGCAAGGCTCTTGTGATCACCAATTCAGCTAGAAAATCGTCCACA GTTGGAGAGATTGTCAACCTCATGTCCGTGGATGCCCAGCGCTTCATGGACTTGGCCACGTATATTAACA TGATCTGGTCAGCCCCTCTGCAAGTCACCCTAGCCCTCTACTTCCTGTGGCTGAACCTGGGCCCTTCTGT GCTGGCTGGGGTGGCTGTTATGATCCTCATGGTGCCCTTCAATGCTGTGATGGCCATGAAGACCAAGACT TACCAGGTGGCACACATGAAGAGCAAAGACAACCGAATCAAGCTGATGAACGAGATCCTCAATGGGATCA AAGTACTCAAATTGTACGCCTGGGAGCTGGCTTTCCAGGACAAAGTTATGAACATCAGGCAGGAGGAGCT GAAGGTGCTGAAGAAATCCGCCTACCTGGCGGCTGTGGGCACATTCACATGGGTTTGCACACCTTTCCTG GTGGCTCTGTCAACCTTTGCTGTCTTTGTGACTGTGGACGAGAAGAACATCCTAGATGCAAAGAAAGCCT TTGTATCCCTAGCCCTGTTCAATATCTTGCGCTTCCCACTCAACATCCTACCCATGGTCATCAGCAGCAT TGTGCAGGCCAGCGTGTCCCTCAAGCGTCTCAGGATCTTTCTGTCTCACGAGGAGCTGGAGCCAGACAGC ATCGAGCGATGGTCGATCAAGGATGGTGGAGGGATGAATAGCATCACTGTGAAGAATGCAACCTTCACTT GGGCCAGGGATGAACCTCCCACACTGAATGGCATCACCTTCGCCATCCCTGATGGAGCCCTTGTGGCCGT GGTGGGCCAGGTAGGCTGTGGGAAGTCATCTCTGCTGTCAGCCCTGCTGGCTGAGATGGACAAAGTGGAG GGACATGTGACTCTCAAGGGCTCCGTGGCCTATGTGCCCCAGCAGGCCTGGATTCAGAATGACTCTCTCC GAGAGAACATACTGTTTGGGCGCCCCCTGCAGGAACATTGCTACAAGGCGGTGATGGAAGCCTGTGCCCT CCTTCCGGATTTGGAAATCCTTCCCAGTGGGGACCTCACAGAGATTGGTGAGAAGGGTGTGAACCTGTCG GGGGGCCAGAAGCAGCGTGTGAGCCTGGCTCGGGCTGTGTATTGTAACTCTGACATCTACCTCTTGGACG ACCCCCTCTCGGCTGTGGATGCACATGTTGGGAAGCACATCTTTGAGAAGGTGGTGGGTCCCATGGGCCT ACTGAAGAACAAGACACGGATCCTGGTCACCCATGGTATCAGCTACCTGCCCCAAGTGGATGTCATCATT GTCATGAGTGGCGGCAAGATCTCAGAGATGGGATCTTATCAGGAGCTGCTAGACCGGGATGGGGCCTTTG CTGAGTTCGTGCGCACCTATGCCAACACTGAGCAGGACCTGGCTTCAGAGGATGACAGTAAGAATGGTGT CAGTGGTTTAGGGAAGGAGTCAAAGCCGGTGGAAAATGGGATACTGGTGACAGACGCAGTAGGGAAGCCC CTGCAGAGGCATCTCAGCAACTCTTCTTCCCACAGTGTGGTTACTAACCAGCAGCACAGCAGCACAGCCG AGCTGCAGAAGTCTGGAGTTAAGGAGGAGACTTGGAAGCTGATGGAAGCAGACAAGGCCCAGACAGGGCA GGTGAAGCTTTCCGTGTACTGGAACTACATGAAGGCCATTGGCCTCTGCATCTCCTTCTTGAGTATCTTC CTTTTCCTGTGCAATCATGTATCTGCACTGGCTTCTAACTATTGGCTGAGTCTCTGGACAGATGACCGCC CTGCTGTCAATGGGACTCAGGAGAACAGGAATTTTCGACTAAGTGTCTATGGGGCCTTGGGCATCTTGCA AGGTGTGGCAGTATTTGGCTATTCCATGGCTGTGTCCATTGGGGGCATCTTTGCCTCCCGTCGCCTGCAC CTAGACCTGCTACAGAATGTCCTGCGATCACCCATGAGTTTCTTTGAGCGTACACCCAGTGGGAACCTAG TGAACCGATTCTCCAAGGAGTTGGACACAGTGGACTCCATGATCCCGCAGGTCATCAAGATGTTCATGGG TTCACTCTTCAGTGTCATTGGAGCTGTCATCATCATCCTACTGGCTACGCCCATTGCCGCAGTCATCATC CCACCCTTGGGTCTGGTTTACTTCTTTGTGCAGAGGTTCTATGTGGCCTCCTCTCGACAGCTGAAGCGCC TGGAGTCTGTCAGTCGTTCCCCTGTGTACTCACACTTCAATGAGACCTTGCTGGGGGTCAGTGTCATCCG TGCCTTTGAGGAACAGGAGCGCTTCATTCGCCAAAGTGACCTGAAAGTAGATGAGAACCAGAAGGCCTAC TACCCCAGCATTGTGGCCAACAGGTGGCTTGCTGTGCGCCTGGAGTGTGTGGGCAACTGCATTGTGCTGT TTGCTGCCCTTTTCGCAGTCATCTCCCGGCATAGCCTCAGTGCTGGCTTGGTGGGTCTCTCTGTGTCTTA CTCACTGCAGATAACTGCATACTTGAACTGGCTAGTTCGAATGTCCTCTGAGATGGAGACCAACATTGTG GCAGTGGAGAGACTGAAGGAATATTCTGAAACGGAGAAGGAGGCTTCTTGGCAAATCCAAGAGACAGCTC CACCCAGCACCTGGCCCCATTCAGGCCGTGTAGAGTTCCGGGATTACTGCTTGAGGTATCGAGAAGACTT GGACTTGGTTCTCAAGCACATAAATGTCACCATTGAGGGTGGAGAAAAGGTTGGTATTGTGGGTCGTACA GGAGCTGGGAAATCATCTCTCACCCTGGGTTTGTTCCGGATCAATGAGTCTGCAGAAGGGGAGATCATCA TTGATGGGATAAACATTGCTAAGATTGGCCTGCACAACCTGCGCTTCAAGATCACCATCATTCCACAGGA TCCTGTTTTGTTCTCGGGTTCCCTCCGCATGAACCTGGACCCTTTCAGTCAGTATTCTGATGAAGAAGTC TGGATGGCTCTGGAGCTTGCTCACCTGAAGGGCTTTGTGTCAGCCTTGCCTGACAAGCTGAACCATGAGT GTGCAGAAGGTGGAGAGAATCTGAGTGTGGGGCAGCGACAGCTTGTGTGCCTGGCCCGGGCTTTGCTGAG GAAGACAAAGATTCTAGTGTTGGACGAGGCTACAGCAGCTGTGGATCTGGAGACAGATGACCTTATTCAG TCCACCATCCGGACGCAGTTTGAAGACAGTACTGTGCTCACTATTGCTCATCGGCTGAATACCATAATGG ACTATACAAGGGTGATTGTCCTGGACAAAGGAGAAATTCGGGAGTGTGGTGCACCCTCTGAGCTCCTGCA GCAAAGAGGCGTCTTCTATAGCATGGCCAAGGATGCTGGCTTGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022281
Insert Size	4599 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022281.2, NP_071617.2
RefSeq Size	4981 bp
RefSeq ORF	4599 bp
Locus ID	24565
UniProt ID	Q8CG09
Gene Summary	ATP-binding cassette (ABC) multiple drug resistance protein involved in the release of glutathione synthase during oxidative stress [RGD, Feb 2006]

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