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Cblc (NM_001034920) Rat Untagged Clone
CAT＃: RN201072
Cblc (untagged ORF) - Rat Cas-Br-M (murine) ecotropic retroviral transforming sequence c (Cblc), (10 ug)
CNY 6,460.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC124804 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201072 representing NM_001034920
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGCTACAGCCCCGCAAGGGTGGCAGTGGGGAGAGCCACGCGCGCTTGGCCGGGCGGTAAAGT TGCTCCAGCGCCTGGAAGAGCAATGCAGGGATCTCAGGCTGTTCGTGGGGCCCCCGTCGCTACGGGACCT GCTACCCCGCACCGCACAGCTACTTCGAGAGGTGGCAAAAGCCCGGAGCGATAAGACCAGGGGAGACCCC GAGGGTCCCGGAGGCGCCGGTGATTTTCTGGTTATCTACCTGACCAACCTGGAGGCCAAAGGCAGGCAGG TGGCTGAGCTCCTGCCACACCGCGGCAAAAAGGACGCGAACCAGGATGTTTTCCCGGAGGGCTCCAGATT CAGGCGACAACTGGCCAAGCTGGCTCTCATCTTCAGTCACATGCATGCGGAGTTGAGCGCACTCTTCCCT GAGGGCAAGTACTGTGGGCACCTGTACCAGATCACCAAGGGCTCTGCCAACACCTTCTGGAGGGAGAATT GTGGAGTTCGGTGCGTCCTTCCCTGGGCTGAGTTCCAGTCCCTCCTGTGCTCCTGCCACCCTGTGGAACC AGGCCCCATCATGCAGGCCTTGCGGTCCACCTTGGACCTCACCTGTAGCGGCCACGTGTCTGTCTTTGAG TTCGACATCTTCACCAGGCTCTTCCAGCCGTGGCCCACTTTACTGAAGAATTGGCAACTCCTGGCTGTCA ACCATCCTGGCTACATGGCCTTCCTCACCTACGATGAGGTCCAAACACGTCTGCAGGCCTACAGGGACAA ACCGGGCAGCTATATCTTCCGGCCCAGCTGTACCCGCCTAGGGCAGTGGGCCATTGGGTACGTGAGCTCC AATGGAAGCATCCTACAGACCATTCCTCTCAACAAGCCTCTGCTCCAGGTGCTCCTGAAAGGACAAAAGG ACGGCATCTTCCTCTACCCTGATGGAAAGAACCACAACCCAGACTTGACTGAGCTCTGCCGTGCAGAACT GAACCAGTGCATCCAAGTGTCACAGGAACAACTGCAGCTCTACCAAGCCATGAACTCCACCTTTGAGCTG TGCAAGATCTGCACAGAGAGGGACAAGGACGTGAGAATTGAGCCCTGTGGGCATCTTCTCTGCAGCTGCT GCCTGGCTGCCTGGCAGCACTCAGACAGCCAGACCTGTCCCTTCTGCCGCTGTGAGATCAAAGGCCGAGA GGCTGTGAGTATCTGTCAGGCACAGGAGAGGTCAATGGAGGTCAGGACTACTGCAGGTGACTCAGGAGAC AACTGTCACCAGGAGGCTGCCGAATGGAAACTGGAATCGGTGACTCCTTCTGCTCCCCCACTGCCCCCTG AAGTTCCATGCCCTCAGAGACCCCAAGACAAAGGCTGGCTGACACTGGCACCGTTCACCCTGCCCAGACT CCGACCTCCACTTCCTCTCCCCAAAATGGCATCAGTTCTGTGGGAAGTCACCTCCAGGCCCCAGGTCAGG GAGGGGGCCACAGAAAGCTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001034920 |
| Insert Size | 1494 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001034920.2, NP_001030092.1 |
| RefSeq Size | 1712 bp |
| RefSeq ORF | 1494 bp |
| Locus ID | 292699 |
| UniProt ID | G3V8H4 |
| Gene Summary | Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Functionally coupled with the E2 ubiquitin-protein ligases UB2D1, UB2D2 and UB2D3. Regulator of EGFR mediated signal transduction; upon EGF activation, ubiquitinates EGFR. Inhibits EGF stimulated MAPK1 activation. Promotes ubiquitination of SRC phosphorylated at 'Tyr-419', has the highest ubiquitin ligase activity among CBL family proteins. In collaboration with CD2AP may act as regulatory checkpoint for Ret signaling by modulating the rate of RET degradation after ligand activation; CD2AP converts it from an inhibitor to a promoter of RET degradation; the function limits the potency of GDNF on neuronal survival.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201072 | Cblc (Myc-DDK-tagged ORF) - Rat Cas-Br-M (murine) ecotropic retroviral transforming sequence c (Cblc), (10 ug) |
CNY 6,460.00 |
|
| RR201072L3 | Lenti ORF clone of Cblc (Myc-DDK-tagged ORF) - Rat Cas-Br-M (murine) ecotropic retroviral transforming sequence c (Cblc), (10 ug) |
CNY 8,360.00 |
|
| RR201072L4 | Lenti ORF clone of Cblc (mGFP-tagged ORF) - Rat Cas-Br-M (murine) ecotropic retroviral transforming sequence c (Cblc), (10 ug) |
CNY 9,030.00 |
