Synm (NM_001134858) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Dmn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201120 representing NM_001134858 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCCTGGCGGCTGCAGACCAGCTCCGAAAAGGCCGAGCTCCAGGAGCTCAATGCCCGGCTTTATG AGTACGTGTGCCGGGTGCGAGAGCTGGAGCGCGAGAACCTGCTGCTGGAGGAGGAGCTGCGCAGCCGGCT GAGCCGGGAGGACCGCTGGGCCGAAGATCAAGCGCTCTACGCCGAGGAGGCGCGCAGCTTGCGACAGCAG CTCGACGAACTGAGCTGGTCCACGGCGCTGGCCGAGGGCGAGCGCGACGCGCTGCGGAGGGAACTGCTGG ACCTGCAGCGGGAAGGTTTGGAAGCCAGCACTGCCCGCGGCCGCCTGGATGCCGAGCTAGAAGCGCAGCG GCGGGAGCTGGAGGAGGCGCTGGGCGCGCGCGCCGCCCTCGAGGCGCTGCTGGGCCGGCTGGAGGCAGAG CGACGAGACCTGGATGCGGCCCACGAGCGCCAAGTGCGAGAGCTACGCGCCCGTGCCAGCAGCCTCACCA TGCACTTCCGAGCCCGCGCCACCGGCCCCGCCGCGCCCCCGCCGCGCCTGCGGGACGTGCACGACAGCTA CGCCCTGCTAGTGGCCGAGTCGTGGAGGGAGAGCATGCAGCTGTACGAGGACGAGGTGCGCGAGCTGGAG CAGGCGCTGCGCCGCGGTCAAGAGAGCCGGCTCCAGGCGGAGGACGAGGCGCGGCTGTGCGCGCAGGAGG CGGACGCGCTGCGGAACCAGGCGCTGGAGTTGGAGCAGCTGCGTGCGAGGCTGGAGGATGAGCTGCTTCG GATGCGCGAGGAGTACGGGATGCAGGCGGAGGAGCGGCAGAGAGTGATCGACAGCCTGGAGGATGAGAAG GATGCCCTTACCTTGGCCATGGCTGACAGGCTGCGGGAGTACCAGGAGCTCCTACAGGTGAAGACGGGCC TCAGCTTGGAGGTAGCTACCTACAGGGCCTTGTTGGAAGGAGAAAGCAATCCGGAGATATTGATCTGGAC CGAGAACATTGAAAACGTGCCACGAGAGTCCAGAAACACATCCTATCGCTATACCGACTCAGTATTACAG AGGAAGAATGAGAAGAATCTCTTTCCAAGGCGAAAAACACCTTGGGCAGCTGTAAATCACAGCTCGACTT TGTATTCCAACGGGCCAGGGCACCTTGACTCACAGACAACCACAGCTGTTGGAAATGCTGCCCGAAGGGG CTTCCTGACCTCCTGCTATTCTTCCTCAGCTACAACCTCACAGCAGAAGCCCCTGGAGAAAACTGTCAGC AGTCAAGCCAGTTTAAGACCAGTCATACCAACCCATGGCTTTCTAAGAAACACTGATGCTCAAGTGAAAA CATTCTCCCATGGACCCAAAGCTGAAGGCATGGGGGACACCCTCACACGGCGGGCCACAGAGTCCGTTAT TACCAGAGAGTCATACAGAGGTCACCAGGGCAACGTGGCGGCAGGTGCTGTGAACAGCACTCAGTCAAAT GAGAGGACTGTCATTCTGGGAAAGAAATTAGAAACGCAGGCCACTAAGGAGCAAGAGAGGGACAGGTCAG GGGTGGTCAAATTAAAGCCAGAGGAGAAAATGTTTGATTCTAAAGAGAAGGCTTCGGAGGAAAGAAACTT GAGGTGGGAAGAGCTGACCAAGTTAGATGCAAGAGAGAGGGAAAGCCAGCATTTAAGGAACGAGGCCAAA GGGAAGGAAACACTGACGGGGAGAAGTAGAAAAGAAAGAGAAGTGCCCATCAGTCTAGAAGTATCTCGGG GCAGCAAATCAGAGGTGTCCACAATACACTTGCAGTCATCCGGAAGGAAGGATTCTGCCAACAGTAGGGA AAGAGAGGTAAAGACCAAAGAGACAAAGTTCCACTTGGACGGCAAGGACACTGCTGGCTCTCTGCAGAGT GACTCTACGACAGAGACCATAGCAGAAAACATCGTCACCACCATCCTTAAGCAGTTTACCCAGTCTCCAG GTGCTGAAGAGAATGTCAGTTCTTTGCCAGACACAAAGGTCACCTACCTGGACCGGAAAGAGTTTCCTGG TGACAGGAAAACCAAGACGGAAATCGTCATGGAGTCGAAGCTGACAGAAGACGTCGATGTTTCTGATGAA GCTGGCCTGGACTACCTTCTAAGCAAGGATGTCAAGGAAGTAGGACTGAAAGGGAAGTCAACAGAGACAG TGATTGGAGAGATGATCAACCTCGGTCTAAAAGGAAGGGAGGGGAGAGCCAAAGTCGTAAATGTGGAGAT TGTGGAAGAGCCCATGAGCTACGTAGGTGGTGGGAAAGTAGAGTTTTCTACCCCCTTCCAGGTGGAAGAA GTCGATGATGTGTCTCCCAGCCCCAAGGGGTTCATTGAGGAAGAGGAGGGCGAAGGAGAAACCCACATGG CATTCTCAATGCATCAACGTCAGAGGACCAACCAGCCCCAGGTGAACGTCCCTCATGTGGAAGAAGTGAC GGAGGCGGGCGACTCGGAGGGGGAGCAGAGTTATTTCGTGTCTACCCCAGATGAGTACCCTGGGGGACAT GACAGAGAAGATGATGGCTCTGTGTATGGGCAGATCCACATTGAGGAAGAGTCCACCATCCGGTACTCAT GGCAAGACGAGATTGCACAGGGGACTTGGAGAAGGAAAGCGAGAGGTGACACGGGGGAAGAGAAGCACAT GAAGGTCCTGGAGGTTCCATCGGCTTCCCTGGGTGGTGCCTTTGGATCTGCTCACGTGAAGGAAGAAGCT AGTGGTGAACTCCACGCTGAACCCACAATCATTGAGAAAGAGATCAAAATACCGCATGAATTCCACACCT CCATAAAGGGCGTCTTCTCCAGTGAGCCTCGGCATCAGTTGGTGGAGGTCATTGGGCAGCTGGAGGAAAC GTTGCCTGAGCGCATGAAGGAAGAGCTGTCTGCCCTGACCAGACAGAGTCAAGGAGAGCCAGGGAGTGTC TCAGTGGATGTAAAGAAAGTCCAGAGCTCTGCTGGCGGTTCTGTGACCTTAATGGCTGAGGTCAACCTCT CGCAGACTGTAGATGCTGATCAGTTAGACCTGGAGGAGTTGAGCAGGGACGAAGCTGGGGAAATAGAGAA GGCTGTGGAGTCAGTGGTAAGAGAGAGCTTGACCAAGCGCTCCAGCCCAGTGCCTAGAAGCCCAGACAGG GAAGATGGAGAGGAGGCGCCTGCCGGTGGCTTTCTCTTCAAGCGCTGGGCCACCAGGGAGCTGTATAGCC CGTCTGGTGAGAAGGATGATACTGACCGGGTCTTTCACAGCTCGGATCAGCGTGTTACCCAGGGCCCAGT GTCAGCCACCGTGGAAGTGACCAGCCCAACGGGTTTTGTGCAGTCTCATGTGCTGGAGGATGTAAGCCAG TCTGTAAGGCACGTTAAACTAGGCCCCACTGAGATGTGGAGGACGGAACAGGTGTCCTACGGAGGACCCA CCTCACCGGTGGTGGAGGTAAGTGAAGACTTCAGCGAGGCAGTCAGCTCCGAGGGAGCCAGCCGCTCCGT GAGGCACGTTACACTGGGTCCCCGTCAAAACCGAGTGTCCACAGAAGTCATTTTCCGAGGCTCTGTGCCT ACCGGGCAGGAGACAGGAGACACAGGAGAGCCTGACCCTGCAGCACTGTCTGTGGAAGCTGACATCTCAG GGAGTGGCAGGATGCCCGGCTCTCAGCAGGCTCACAATGAAAAGGAAACTCCTTTTCCGGGTCCTATTTC TGGGGCAGCCCAGGTTGGTAGTAACTTTTCAACTGAAGGGTCAGTGGGTACCCAGACTTTTGTTAGGCAT CTCCAGTTAGGCTCCAGAGAAGGGTTCAGAGAAGAAATCCAGTTCATAGCTCCCATCCCAGACAGGGCAG AGTGGGGAGAAGAGGATTCTGGGTACACCAGAGTGTCTCTAGGGAGGAGCACCTCCATCCAGCACATTGA CATCGTGCCTCAGAGGTACCAGGCTTCCAAGCAGGTGGCCCCACAGACATTGGGATTTAGGGACTCAGAA GGCGTAGTCCTGGTGGAGAGGTCAGCAGGTATGACCCAGGCCACTCACAATTTCACCTCAGATAGAGAAA TCCTCAAGAAGGAAGAGAACACATTCCAGAGGGTCATTTCTGGGTCCCCTCCAGACAGTGTGGGCGACAC AGGAGCAGAGGTGACAGAAAACATCAGCAGATCCTTCAGGCACATTCAAATAGGTCCTACAGAGGAGGAC CCCTCTGAATACATTGTCACCAGTGGACCCTTGTCTAAAACATTTGTTCTTGACAGTTCCGTGGCATCCC CTGGGCCGGGTGGGGGAGCAGACGGCAGTAGCACACCATGTCACATTGCACTGGGGCCCAAAGAAACTTC ATTTACCTTTCAGATGGGTGTAAGTGACACGAGAGCCATCCGCAGCTGGACCCGAGACACAGGGTCAGAA GTGGAAGCTCACAGTGTGTCTCACCACGGTGGCTGGAGAGTTGCTCACAGTAGGGATGAGCAATCAGCCA GCACCAGCTCTCAGGCCTCTGCTGGGGATGCACACCAGGCCCGTGGAGAGAAAGGCACGGAGCAGGCTGG ATTTGACAAGACGGTGCAGCTGCAAAGAATGGTAGACCAAAGGTCTGTGGCTTCAGATGAGAAGAAAGTG GCCCTTCTCTATCTAGACAATGAGGAGGAAGAGGAGGGCGAGGGATGGTTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134858
Insert Size	4674 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134858.3, NP_001128330.1
RefSeq Size	7432 bp
RefSeq ORF	4674 bp
Locus ID	308709
Gene Summary	human homolog is an intermediate filament protein; involved in connecting desmin the extracellular matrix [RGD, Feb 2006]

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