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Plin5 (NM_001134637) Rat Untagged Clone
CAT＃: RN201130
Plin5 (untagged ORF) - Rat similar to lipid droplet associated protein (LOC501283), (10 ug)
CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Oxpat |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201130 representing NM_001134637
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGAGAGGCAAAGACACCACCCTAGCTCTACACAGCAGGATGTCCGGTGATCAGACAGCTCAGG ACCCTGGATCCAGCCTGGGGGAACTGGACCAGCACAACGTGGTGAAGCGAGTGGTGGCCTTGCCCCTGGT CAGGGCCACGTGCACTGCTGTGTCTGGTGCCTACAACTCAGCCAAGGACAGGCACCCGCTGCTGGGCTCT GCCTGCCGCTTTGCTGAACACTGTGTGTGTAGTGTGGCTACCTGTGCCCTGGACCACGCACAGCCACTGC TGGAGCACCTGCAGCCCAAGTTGGCCACAGTGAATGATCTTGCCTGCAGGGGACTAGACAAATTGGAAGA GAAGCTGCCCTTCCTGCAGCAGCCATCGGACACGGTGGTCACCTCAGCCAAGGATGCAGTGGCCAAAAGT GTCACAGGCGTGGTGGACCTGGCCCAAAGGGGCCGGCGTTGGAGCGGGGAGCTGAGGCGCTCCGTGAGCC AAGCCATGGACACTGTGCTGAGGCGCTCCGTGAGCCAAGCCATGGACACTGTGCTGGGCAAATCAGAGGA GCTGGTGGACCATTTCCTGCCCATGACTGAAGCTGAGCTAGTGGCCCTGGCGACTGAGTCCCAGGGCCCA GAAGTGGGCTCAGTGGAGGAGCAGAGGCAGAAACAGGGCTACTTTGTGCGTCTGGGATCCCTATCGGCAC GCCTCCGCCATCTTGCCTATCAACACTCTTTGGGGAAACTGAGGGAGAGCAAACACCGCACCCAGGAGAT GCTGGCCCAGCTGCAGAAAACTCTGGAGCTGATCCAGCATATGCAGAGTAGGGCAAGCCCCACCCCTACT TTCCATCACCCAAAGGTCCAGGAGCTTTGCGGGGACTGGAGCCCATGTCTGGAGAATGGCTACCGCCACA GCCAGGTGGAGCTGGAGACACTGGCTCTGTCGAGAAGTCTGACCCTGGAGCTGCAGAGTGCAGTGGATGC CCTTGCAGGCTGTGTTCGGGGCCTGCCCCCTAGTGCCCAGGCCAAGGTGGCTGAAGTGCAGCGCAGTGTG GATGCTCTACAGGCCACCTTTGCTGATGCACACTGCCTTGGTGACGTGGCACCCACTGCTCTGGCTGAGG GCCAGGACAGCGTGGCCCAGGCACATGCCTGTGTGGATGAGTTCCTGGATTTGGTCCTTCGAGCCATGCC ACTGGCCTGGCTTGTGGGGCCCTTTGCGCCCATCTTGGTGGAGCGGTCAGAGCCCCTGATCAACCTGGCC ACCTGTGTGGATGAGGTGGTGGGTGACCCTGACCCCCGCTGGGCACACATGGACTGGCCAGCCCAGCAGA GGGCCTGGGAGGCTGAGCCTGCAGACCCTGGGGGACAAGAGGCTGAGCCCCCACTGGGCCAGGTCAAGCA CACAATGATGCCAGAGCTAGACTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001134637 |
| Insert Size | 1428 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001134637.1, NP_001128109.1 |
| RefSeq Size | 1937 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 501283 |
| UniProt ID | M0R7Z9 |
| Gene Summary | Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201130 | Plin5 (Myc-DDK-tagged ORF) - Rat similar to lipid droplet associated protein (LOC501283), (10 ug) |
CNY 6,180.00 |
|
| RR201130L3 | Lenti ORF clone of Plin5 (Myc-DDK-tagged ORF) - Rat similar to lipid droplet associated protein (LOC501283), (10 ug) |
CNY 7,980.00 |
|
| RR201130L4 | Lenti ORF clone of Plin5 (mGFP-tagged ORF) - Rat similar to lipid droplet associated protein (LOC501283), (10 ug) |
CNY 8,840.00 |
