Flt1 (NM_019306) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	FLT-1; VEGFR-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201567 representing NM_019306 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCAGCTGCTGGGACACCGCGGTCCTGCCTTGCGCGCTGCTCGGGTGTCTGCTTCTCACAGGATATT GTTCAGGGTCGAAGTTAAAAGGACCAGAACTGAGTTTAAAAGGCACCCAGCACGTCATGCAAGCGGGCCA GACTCTCTTTCTCAAGTGCAGAGGGGAGGCAGCCCACTCCTGGTCTCTGCCTACTACAGTGAGCCAGGAG GACAAAAAGCTGAGCGTCACTAGATCAGCCTGTGGAAGGAATAACAGACAATTCTGCAGCACCTTGACCT TGAACATGGCGCAGGCCAACCACACGGGCCTCTACTCCTGCAGATACCTGCCTAAATCCACCTCGAAGGA AAAGAAAATGGAATCTGCAATCTACATATTTGTTAGTGATGCAGGGAGTCCTTTCATAGAGATGCACAGT GACATACCCAAACTCGTGCACATGACAGAAGGAAGAGAGCTCATCATCCCCTGCCGGGTGACGTCTCCCA ACATCACAGTCACTCTAAAAAAGTTTCCATTTGACGCTCTTACCCCTGACGGGCAAAGAATAGCGTGGGA CAGTAGGAGAGGCTTTATAATAGCAAATGCAACGTACAAAGAGATAGGACTGCTGACCTGCGAAGCCACA GTTAACGGGCACCTGTACCAGACAAGTTATCTGACCCATCGGCAGACCAATACAATCCTAGATGTCCAAA TAAGCCCACCGAGCCCCGTGAGATTCCTCCGTGGTCAAACTCTTGTCCTCAACTGCACCGTCACCACGGA CCTCAATACAAGGGTGCAGATGAGCTGGAATTACCCTGGTAAAGCAACTAAGAGAGCATCTATCAGGCAG CGGATTGACCAAAGCAATCCCCACAGCAATGTGTTCCACAGCGTTCTTAAGATCAACAACGTGGAGAGCC GGGACAAGGGACTCTACACTTGTCGTGTGAAGAGTGGGTCGTCATTCCGGACTTTCAACACCTCTGTGCA TGTGTATGAAAAGGGATTTATCAGCGTGAAGCATCGGAAGCAACAGGTGCAGGAAACCATAGCAGGAAAA CGGTCCCATCGGCTGTCCATGAAAGTGAAGGCCTTCCCCTCGCCAGAAGTCGTATGGTTAAAAGATGGCG TACCCGCAACGGAGAAATCTGCTCGCTATTCGGTGCATGGCTACTCGTTAATTATCAAAGATGTAACTGC CGAGGACGCAGGGGACTATACAATCTTGCTGGGCATAAAACAGTCAAAGCTATTTAGAAACCTCACAGCC ACTCTCATCGTAAATGTGAAACCTCAGATCTACGAAAAGTCCGTGTCGTCCCTTCCAAGCCCACCTCTCT ACCCACTGGGCAGCAGACAAGTCCTCACTTGCACCGTGTATGGCATCCCTCAGCCTACCATCAAGTGGCT GTGGCACCCCTGTCACTACAACCACTCCAAAGAAAGGAATGACTTCTGCTTTGGGAGTGAAGAATCCTTC ATCCTGGATTCCAGCAGCAACATAGGAAACAGAATCGAGGGCATCACTCAGCGCATGATGGTCATAGAAG GAACCAATAAGACGGTTAGCACATTGGTGGTGGCTGACTCTCGGACCCCTGGAATCTACAGCTGCAAGGC CTTCAATAAAATAGGGACTGTGGAGAGAGACATAAGGTTTTACGTCACAGATGTGCCAAACGGCTTTCAC GTTTCCTTGGAAAAGATACCAACCGAAGGAGAGGACCTGAAACTGTCCTGTGTGGTCAGTAAATTCCTGT ACAGAGACATTACCTGGATCCTGCTACGGACAGTTAACAACAGGACCATGCACCATAGCATCAGTAAGCA AAAAATGGCCACCACTCAGGACTACTCCATCACTCTGAACCTTGTCATCAAGAATGTGTCTCTGGAAGAC TCGGGCACCTATGCCTGCAGAGCCAGGAACATATACACAGGGGAAGAGATCCTTCGGAAGACAGAAGTTC TCGTTAGAGATTTGGAAGCGCCACTCCTGCTTCAAAACCTCAGTGACCACGAGGTGTCCATCAGTGGCTC CACGACCTTAGACTGTCAAGCTAGAGGTGTCCCTGCGCCTCAGATCACTTGGTTCAAAAACAACCACAAA ATACAACAAGAACCGGGAATTATTTTAGGACCAGGAAACAGCACGCTGTTTATTGAAAGAGTCACAGAAG AGGATGAGGGTGTCTATAGGTGCCGAGCCACCAACCAGAAGGGGGTCGTGGAAAGCTCAGCGTACCTCAC CGTGCAAGGAACCTCAGACAAGTCAAACCTGGAGCTGATCACCCTCACGTGCACGTGTGTGGCTGCGACA CTCTTTTGGCTCCTTCTAACTCTCTTCATCCGAAAACTGAAGCGGTCTTCTTCCGAAGTAAAGACGGACT ACCTGTCAATCATCATGGACCCAGATGAAGTCCCCCTGGATGAGCAGTGTGAACGGCTGCCCTATGATGC CAGCAAGTGGGAGTTTGCGCGGGAGAGACTTAAACTAGGCAAATCACTCGGGAGAGGGGCTTTTGGGAAG GTGGTTCAGGCCTCGGCATTTGGCATTAAGAAATCACCCACCTGCCGGACTGTGGCTGTGAAGATGTTGA AAGAGGGGGCCACAGCCAGTGAGTACAAAGCTCTGATGACCGAACTCAAGATCTTGACCCACATCGGCCA TCATCTGAATGTGGTTAACCTCCTGGGAGCCTGCACCAAGCAAGGAGGGCCTCTGATGGTGATCGTGGAA TACTGCAAATATGGAAACCTGTCCAACTACCTTAAGAGCAAACGTGACTTCTTCTGTCTCAACAAGGATG CAGCCTTGCATATGGAGCCCAAGAAAGAAAAGCTGGAGCCAGACCTGGAGCAGGACCAGAAACCCCGCCT AGACAGTGTCAGCAGCAGCGAGAGTTTCACCAGCTCCGGCTTCCAGGAAGATAAGAGTGTGAGCGATGTG GAAGGAGGCGAGGATTACAGTGAGATCTCCAAGCAGCCCCTCACCATGGAAGACCTGATCTCCTACAGTT TCCAAGTGGCCAGAGGCATGGAGTTTCTGTCCTCCAGAAAGTGCATTCATCGCGACCTGGCAGCACGGAA CATCCTTTTATCTGAGAACAATGTTGTGAAGATTTGCGACTTTGGCCTGGCCCGGGATATTTATAAGAAC CCTGATTACGTGAGGAGAGGAGATACTCGACTTCCCCTAAAATGGATGGCTCCCGAATCCATCTTTGACA AGGTCTACAGCACCAAGAGTGACGTGTGGTCCTACGGAGTGTTGCTGTGGGAGATCTTTTCCTTAGGGGG TTCTCCATACCCAGGAGTGCAAATGGATGAAGACTTCTGTAGCCGGCTGAAGGAAGGCATGCGCATGAGA ACACCAGAGTATGCCACACCTGAAATCTACCAAATCATGCTGGATTGCTGGCACAAAGACCCCAAAGAAA GGCCCCGGTTTGCTGAACTTGTGGAGAAGCTCGGTGACCTGCTTCAAGCCAATGTCCAACAGGATGGTAA AGACTACATCCCCCTCAACGCCATACTGACTAGAAACAGTGGCTTCACATACTCAGTCCCCACCTTCTCA GAGGACTTTTTCAAGGATGGTTTCACGGATCCAAAGTTTCATTCTGGAAGCTCTGATGATGTGAGATACG TAAACGCTTTCAAATTCATGAGCCTGGAAAGAATCAAAACCTTTGAGGAGCTTTCACCAAATGCCACCTC CATGTTTGAAGACTATCATCTGGATACCAGCAGTCTGCTGACCTCCCCCTTGCTGAAGCGATTCACCTGG ACTGAGACCAAGCCCAAGGCCTCAATGAAAATAGACCTGAGAATTACTAGTAAAAGCAAGGAGGCGGGGC TTTCCGATCTGCCCGGACCCAGCTTCTGCTTTTCCAGCTGTGGCCACATCAGGCCTGTGCGCCAGGAGGA TGAAGACGACCCTGAGCTGGGGAAGGAATCCTGCTGCTCTCCGCCCCCAGACTACAACTCCGTGGTGTTG TACTCCTCCCCGCCTGCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019306
Insert Size	4011 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019306.2, NP_062179.2
RefSeq Size	6848 bp
RefSeq ORF	4011 bp
Locus ID	54251
Gene Summary	tyrosine kinase receptor for vascular endothelial growth factor; may play a role in cell proliferation and cell survival [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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