Flt1 (NM_019306) Rat Untagged Clone

CAT#: RN201567

Flt1 (untagged ORF) - Rat fms-related tyrosine kinase 1 (Flt1), (10 ug)



  "NM_019306" in other vectors (1)

CNY 21,570.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms FLT-1; VEGFR-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN201567 representing NM_019306
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCAGCTGCTGGGACACCGCGGTCCTGCCTTGCGCGCTGCTCGGGTGTCTGCTTCTCACAGGATATT
GTTCAGGGTCGAAGTTAAAAGGACCAGAACTGAGTTTAAAAGGCACCCAGCACGTCATGCAAGCGGGCCA
GACTCTCTTTCTCAAGTGCAGAGGGGAGGCAGCCCACTCCTGGTCTCTGCCTACTACAGTGAGCCAGGAG
GACAAAAAGCTGAGCGTCACTAGATCAGCCTGTGGAAGGAATAACAGACAATTCTGCAGCACCTTGACCT
TGAACATGGCGCAGGCCAACCACACGGGCCTCTACTCCTGCAGATACCTGCCTAAATCCACCTCGAAGGA
AAAGAAAATGGAATCTGCAATCTACATATTTGTTAGTGATGCAGGGAGTCCTTTCATAGAGATGCACAGT
GACATACCCAAACTCGTGCACATGACAGAAGGAAGAGAGCTCATCATCCCCTGCCGGGTGACGTCTCCCA
ACATCACAGTCACTCTAAAAAAGTTTCCATTTGACGCTCTTACCCCTGACGGGCAAAGAATAGCGTGGGA
CAGTAGGAGAGGCTTTATAATAGCAAATGCAACGTACAAAGAGATAGGACTGCTGACCTGCGAAGCCACA
GTTAACGGGCACCTGTACCAGACAAGTTATCTGACCCATCGGCAGACCAATACAATCCTAGATGTCCAAA
TAAGCCCACCGAGCCCCGTGAGATTCCTCCGTGGTCAAACTCTTGTCCTCAACTGCACCGTCACCACGGA
CCTCAATACAAGGGTGCAGATGAGCTGGAATTACCCTGGTAAAGCAACTAAGAGAGCATCTATCAGGCAG
CGGATTGACCAAAGCAATCCCCACAGCAATGTGTTCCACAGCGTTCTTAAGATCAACAACGTGGAGAGCC
GGGACAAGGGACTCTACACTTGTCGTGTGAAGAGTGGGTCGTCATTCCGGACTTTCAACACCTCTGTGCA
TGTGTATGAAAAGGGATTTATCAGCGTGAAGCATCGGAAGCAACAGGTGCAGGAAACCATAGCAGGAAAA
CGGTCCCATCGGCTGTCCATGAAAGTGAAGGCCTTCCCCTCGCCAGAAGTCGTATGGTTAAAAGATGGCG
TACCCGCAACGGAGAAATCTGCTCGCTATTCGGTGCATGGCTACTCGTTAATTATCAAAGATGTAACTGC
CGAGGACGCAGGGGACTATACAATCTTGCTGGGCATAAAACAGTCAAAGCTATTTAGAAACCTCACAGCC
ACTCTCATCGTAAATGTGAAACCTCAGATCTACGAAAAGTCCGTGTCGTCCCTTCCAAGCCCACCTCTCT
ACCCACTGGGCAGCAGACAAGTCCTCACTTGCACCGTGTATGGCATCCCTCAGCCTACCATCAAGTGGCT
GTGGCACCCCTGTCACTACAACCACTCCAAAGAAAGGAATGACTTCTGCTTTGGGAGTGAAGAATCCTTC
ATCCTGGATTCCAGCAGCAACATAGGAAACAGAATCGAGGGCATCACTCAGCGCATGATGGTCATAGAAG
GAACCAATAAGACGGTTAGCACATTGGTGGTGGCTGACTCTCGGACCCCTGGAATCTACAGCTGCAAGGC
CTTCAATAAAATAGGGACTGTGGAGAGAGACATAAGGTTTTACGTCACAGATGTGCCAAACGGCTTTCAC
GTTTCCTTGGAAAAGATACCAACCGAAGGAGAGGACCTGAAACTGTCCTGTGTGGTCAGTAAATTCCTGT
ACAGAGACATTACCTGGATCCTGCTACGGACAGTTAACAACAGGACCATGCACCATAGCATCAGTAAGCA
AAAAATGGCCACCACTCAGGACTACTCCATCACTCTGAACCTTGTCATCAAGAATGTGTCTCTGGAAGAC
TCGGGCACCTATGCCTGCAGAGCCAGGAACATATACACAGGGGAAGAGATCCTTCGGAAGACAGAAGTTC
TCGTTAGAGATTTGGAAGCGCCACTCCTGCTTCAAAACCTCAGTGACCACGAGGTGTCCATCAGTGGCTC
CACGACCTTAGACTGTCAAGCTAGAGGTGTCCCTGCGCCTCAGATCACTTGGTTCAAAAACAACCACAAA
ATACAACAAGAACCGGGAATTATTTTAGGACCAGGAAACAGCACGCTGTTTATTGAAAGAGTCACAGAAG
AGGATGAGGGTGTCTATAGGTGCCGAGCCACCAACCAGAAGGGGGTCGTGGAAAGCTCAGCGTACCTCAC
CGTGCAAGGAACCTCAGACAAGTCAAACCTGGAGCTGATCACCCTCACGTGCACGTGTGTGGCTGCGACA
CTCTTTTGGCTCCTTCTAACTCTCTTCATCCGAAAACTGAAGCGGTCTTCTTCCGAAGTAAAGACGGACT
ACCTGTCAATCATCATGGACCCAGATGAAGTCCCCCTGGATGAGCAGTGTGAACGGCTGCCCTATGATGC
CAGCAAGTGGGAGTTTGCGCGGGAGAGACTTAAACTAGGCAAATCACTCGGGAGAGGGGCTTTTGGGAAG
GTGGTTCAGGCCTCGGCATTTGGCATTAAGAAATCACCCACCTGCCGGACTGTGGCTGTGAAGATGTTGA
AAGAGGGGGCCACAGCCAGTGAGTACAAAGCTCTGATGACCGAACTCAAGATCTTGACCCACATCGGCCA
TCATCTGAATGTGGTTAACCTCCTGGGAGCCTGCACCAAGCAAGGAGGGCCTCTGATGGTGATCGTGGAA
TACTGCAAATATGGAAACCTGTCCAACTACCTTAAGAGCAAACGTGACTTCTTCTGTCTCAACAAGGATG
CAGCCTTGCATATGGAGCCCAAGAAAGAAAAGCTGGAGCCAGACCTGGAGCAGGACCAGAAACCCCGCCT
AGACAGTGTCAGCAGCAGCGAGAGTTTCACCAGCTCCGGCTTCCAGGAAGATAAGAGTGTGAGCGATGTG
GAAGGAGGCGAGGATTACAGTGAGATCTCCAAGCAGCCCCTCACCATGGAAGACCTGATCTCCTACAGTT
TCCAAGTGGCCAGAGGCATGGAGTTTCTGTCCTCCAGAAAGTGCATTCATCGCGACCTGGCAGCACGGAA
CATCCTTTTATCTGAGAACAATGTTGTGAAGATTTGCGACTTTGGCCTGGCCCGGGATATTTATAAGAAC
CCTGATTACGTGAGGAGAGGAGATACTCGACTTCCCCTAAAATGGATGGCTCCCGAATCCATCTTTGACA
AGGTCTACAGCACCAAGAGTGACGTGTGGTCCTACGGAGTGTTGCTGTGGGAGATCTTTTCCTTAGGGGG
TTCTCCATACCCAGGAGTGCAAATGGATGAAGACTTCTGTAGCCGGCTGAAGGAAGGCATGCGCATGAGA
ACACCAGAGTATGCCACACCTGAAATCTACCAAATCATGCTGGATTGCTGGCACAAAGACCCCAAAGAAA
GGCCCCGGTTTGCTGAACTTGTGGAGAAGCTCGGTGACCTGCTTCAAGCCAATGTCCAACAGGATGGTAA
AGACTACATCCCCCTCAACGCCATACTGACTAGAAACAGTGGCTTCACATACTCAGTCCCCACCTTCTCA
GAGGACTTTTTCAAGGATGGTTTCACGGATCCAAAGTTTCATTCTGGAAGCTCTGATGATGTGAGATACG
TAAACGCTTTCAAATTCATGAGCCTGGAAAGAATCAAAACCTTTGAGGAGCTTTCACCAAATGCCACCTC
CATGTTTGAAGACTATCATCTGGATACCAGCAGTCTGCTGACCTCCCCCTTGCTGAAGCGATTCACCTGG
ACTGAGACCAAGCCCAAGGCCTCAATGAAAATAGACCTGAGAATTACTAGTAAAAGCAAGGAGGCGGGGC
TTTCCGATCTGCCCGGACCCAGCTTCTGCTTTTCCAGCTGTGGCCACATCAGGCCTGTGCGCCAGGAGGA
TGAAGACGACCCTGAGCTGGGGAAGGAATCCTGCTGCTCTCCGCCCCCAGACTACAACTCCGTGGTGTTG
TACTCCTCCCCGCCTGCTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_019306
Insert Size 4011 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_019306.2, NP_062179.2
RefSeq Size 6848 bp
RefSeq ORF 4011 bp
Locus ID 54251
Gene Summary tyrosine kinase receptor for vascular endothelial growth factor; may play a role in cell proliferation and cell survival [RGD, Feb 2006]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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