Tcof1 (NM_001106143) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201779 representing NM_001106143 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGCCAGGAAGCGGCGGGAGCTGCTTCCCCTTATTTACCATCATCTGTTGCAAGCAGGCTACG TCCGCGCGGCGCGGGAAGTTAAGGAGCAGAGCGGCCAGAAGAATTTCCTGACTCATCCCGTCACCCTTCT GGACATCTATACACACTGGCAACAGACCTCAGAGCTCGGCCTGAAGCAGAAGGCAGAGAATGATGAGACA CTGCAAGCCAAGAAGTCTCGAGTGTCAGACCCTATTAGCAGCTCAGAGAGCTCAGAGGAGGAGGAAGAGG AGGCAGAAACGGGAACTGCCAAAGCCACCCCAAGACCGACACCTGTCAATTCCACAGCTGCAGCTTTGCC ATCAAACATGAAACAAAAGGCAAAGACCAAGACAGCCAACAAGACGGTGAACTCCGTGCTGCCCCCTGCG TCCGGAAAGACAGCGGTCCAGCTGCTGTCTGGGAAGTCACCCAAGAAGTCAGCAGAGCCCTTGGCCAGCA TTGTCTTGGCCTCAGAAACTGAGGAGGAGGGCAGTGCCCAAGTCCTGGGACCCACTGCCAAGCCTGGAAT GGTGTCAGCAGGCCAAGCCAGCAGTTCCAGCGAGGATACCTCCAGCTCAAGCGATGAGACAGATGTGGAG GTGAAACCCTCAACAAAACCACAGGCCAAAGCTCCAGTAACTCCTGCCAAGGATCCTCCAGCAAGAACAG CCTCAGGCCCTACCAAGCTAGGGGATGTGACACCCGCATCAGCAAGGGCTGCAGCCACTAAAGCGGCAGA GTCTGAGAGCAGTGAGGAGGACTCAGAGAGTGAGGACGAGGCCCCTGCTGGCCTGCCTAGCCAGGTAACA ACCCCTGGAAAAGGTCTTCATGTCAGAGCTGCCTCAGTTTCTGCCAAGGGGATGTCTAGGAAAGGGCCCA TCTCAGCAACCCCAGAGAAGACTGGGCCCATAACCCCCCAAGCCAAGACAGGAAGGCCACAGAAGGATAT GGAAACCAGCAGTGAGGACGATTCTGACAGTGAGGATGAGATGCCAGTCACTGTGACCACTCCTCAGGCA AGGCCTTCCGGGAAGAGCCCTCAGGTCAGAGGTACCTCTGCCCCTGCCAAGGAGTCATCCCAGAAAGGGG CTCCTGCAGTCACCCCTGGGAAGACAAGACCTGTGGCAGCCCAGGCCCAGGCAGGGAAGCCAGAGACCAG GAGCAGTGAGGAGTCAGAGAGCGACAGTGGAGAGACACCAGCAGCTGGGACTCTAACTACAAGTCCTGCC AAGGTGAAACCTTTGGGGAAAAGCCCCCAGGTCAGACCTGCCTCCACTGCAAACCTGGGGTCATCTAGAA AAGGAGCCAACCCACCCTGCCTTGGGAAGGTGGGGTCAGCAGTTCTCAAGGTCCAAACGGGAAAGGAAGA AGAGGACTCAGAGAGCAGCAGCGAGGAGTCGGACAGTGACGGGGCTGTGAACGCAGCCAAGGCAAAGCCC CCAGGGAGAAAAGCCACTCCAGCACTCCCTCGGAAGACGGGGCCTGTGGCCACCCAGGTCAAGACTGACA AGGGCAAAGACCACGCAGAGAGCAGCGAGGAGTCGACCGACAGCGAGGAGGAGGCAGCACCAGCAGCTTC TGCTGCTCAGGCTAAACCAGCTCCGATAAAGCAGATGAAAGCTTCCCCTAGGAAAGGCACGGCTGCATCC ACCACAGGGGCGAGCGCCTCGTCCCCTCGTAAGGCAGGAACAAAGACCTCTTCAGCCAGCCTATCATCCC TGGCTCTGCCCAAGGGCACCCAGAAGCCAGATGTGGACTCCTCTAGCGAGTGGGAGTCAGAAGGAGCTGC TCCTGGCACCACCGGGGTACAGGGAAAGTCTGGGGGAAAGGGCCTCCAAGGGAGAGCTGCCTCAGGACAA GGGGTGGCCCCACTGCACGCTCAGAAGACAGGGCCTTCAGGTGCCCAGGTCAAAGCCACAGCACAGGAAG ACTCAGAGAGCAGCGAAGAGGAATCCAGCAGTGAAGAAGAGGATGAGACCCCAGCACAGGTCATGGCCTT GGGGAGACTTCCTCCGGCCAAAGCCAACCCACCTCCCACTAAGACACCTCTGGCATCTGCATCTGGAAAA GCGGCCGCAGTAGTTCCTCCACCCAAGGGAAAAGCACCTGCGAGTACTGTTCAGAACAGCACCATCTCTG CCAGGGGCCAGCGGGCTGTGCCGGCCACGGGAAAAGCAGGGGCCCCAGCAACCCAAGCACAGAAGGGGCC CATGGCTGGCACAGGGGAGGACTCAGAGAGCAGCAGTGAAGAGGAGTCTGACAGTGAAGAAGAAACTCCA GCCCAGGTCAAGCCTGTGGGGAAGACCTCTCAGGTCAGAGCTGCCTCGGCCCCTGTCAAGGAGTCTCCTA ACAAAGGAGCTTATTCAGGAACCTCCAGGAAGACAGGGCCTCCAGCTACCCAAGCTCAGACAGGGAAGAC AGAGGACTCAGAGAGCAGTAGTGAGGAATCTGACAGTGACAGAGAGATACCACCAGCCATCACCCCAGCC CAGGATGGTATCTCCCAGTCTACCAGAAGCAAACTCTCAGGTCTGGCACCCCCAGAGAAGAGCACAGAAG AGTCCTCAGAGAGCAGTGATGAGGATCTGCCATCTGGCCAGGCCATTAAATCCCCTCCAATTTCTGTCAA CCCTAATCGTGGTCCAGCTGCCCCAGTTCCAACCCCAGAACAACACCAGGCTGTGAACACAAGGAAGGCC CAGGCCTCAGGCAGCACTGCCCAGAGCTCCTCCTCCGAGAGTGAGGATGAGGACATGATTCCTGCCACAC AACCCCCCACCCTTGCCATCAGAACCAATGTGACTACGCCCACAGCCCTCTCACAAACAGCCGCCCAACC CAGCAAGAGTGAGCAGTCTAGTCGGATGCCAAAAGGCAAGAAACCAAAGACAGCGTCCACTCAGATCAGC AGTGCCATGGAAGCACTCCCCGTGACGCTTCCCCAGAGCACACCTGCCCAGTCCAAAACCACCAACAAGC TGGGGGACCCCAAACTTGCTGAGAAGCAGCAGCTTACCCCAGGCTACCCCAAAGCCCCCAGGAGCTCAGA GGACAGCAGTGACACCTCTTCAGAGAGCGAGGAGGATGCCAAGAGACCCCAGATGTCCAAGTCGTCCCAA AGGCTGGATCCAGACGCTTCCCAGAAGGAAACTGTGGTAGAGGAGACCCCTACAGAATCCAGCGACGACG AGATGGTGGCCCCCTCACAGTCTCTCCTCTCAGGTTATGTGACTCCCGGCTTAACTGTGGCCAATTCCCA GCCTTCAAAAGCTACTCCTAGGCCAGACGCCAACCCCTTGGTTTCCTCTGCTCCAGCCACCAAAGATAAC CCAGATGGCAAGCAGAAATCAAAATCCCAAGACTCCACTGCAGACACCACACTCCGTAAAACCGGTAGGA AAGAGGCCTCCTCAGGATCCACACCTCAGAAGCCCAAGAAGCCCAAGAAGAGCACCTTGAGCTCACCAGC CCCGGCACAGACACTGCCGAACAGCATCACCCAGCGCCTCCTGGAGCAGCCCTGGCCCCTGAGTGAGGCA CAGGTGCAGGCCTCTGTGATGAAGGTCCTGACAGAACTGCTGGAGCAGGAAAGGCAGAAGGCCACCGAGG CCATCAAGGAGAGTGGAAAGAAGGGCCAGAAACGGAAACTATCAGGGGACCAGGTAGAAGCTGGGGCCCC AAAGAACAAGAAGAAGAAGCAGCAGCTTGCGGCTGGGACAAGTGCTGGTTCCCCAGAAAAGGCCTCCAGG ACTTCCAAGGCAAAATCAAAACTAAACAAAGGGAGTGCTGGTGGCAAGGGGAAGGGGTCTCCTGTCCCCC AAGGAGCCAAGGAGAAGCCCGAAGGCAAGCTGGGGATAAAGCTTGAGAGTGGAGAGCAGAGTGACCCGAA GAGCAAGAAGGAAAAGAAGAAATCCAGTAAGAAAAGAAAAAAGGAAAAAAGACCCCAGCCAAGGACTCTG CCTCACCGGTCCAGAAGAAGAAAAAGAAGAAGAAGAAGACAGCCGAGCCTGCTGTGTGAGGGCCAGGCAG CGCATAGAACAGAGTCTTGGCAGAGCGTGACCATCCCCAGGCCTCTGACCTCTGGGCACTGTGAGTTGGG ACTGGACTTTTTAATTTGCCCTCCCTCGGCAGAAGGTGATGGCTGGCGTTGCTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106143
Insert Size	4188 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106143.1, NP_001099613.1
RefSeq Size	4627 bp
RefSeq ORF	4188 bp
Locus ID	291571

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