Shc1 (NM_053517) Rat Untagged Clone

CAT#: RN201790

Shc1 (untagged ORF) - Rat SHC (Src homology 2 domain containing) transforming protein 1 (Shc1), (10 ug)



  "NM_053517" in other vectors (3)

CNY 6,080.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms P66shc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN201790 representing NM_053517
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAGCTGAGTGGAGGCGGCGGGCGCAGGACTCGGGTAGAAGGGGGCCAGCTGGGGGGCGAGGAGT
GGACCAGACACGGGAGCTTTGTCAATAAGCCCACACGAGGCTGGCTGCATCCCAACGACAAAGTCATGGG
ACCTGGGGTTTCCTACTTGGTTCGGTACATGGGTTGCGTGGAGGTCTTACAGTCAATGCGAGCCCTTGAC
TTCAATACCCGGACTCAGGTCACCAGGGAGGCTATCAGTTTGGTGTGTGAAGCTGTGCCTGGTGCCAAAG
GGGCGATGAGGAGGAGAAAGCCCTGCAGCCGCCCACTCAGCTCTATCCTGGGAAGGAGTAACCTGAAGTT
TGCTGGAATGCCGATCACTCTCACTGTGTCTACCAGCAGCCTTAACCTCATGGCAGCAGACTGCAAACAG
ATCATTGCCAACCATCACATGCAATCTATCTCATTCGCGTCTGGTGGGGATCCGGACACAGCTGAGTACG
TTGCCTATGTTGCCAAAGACCCTGTGAATCAGAGAGCCTGCCATATCCTGGAGTGTCCTGAAGGACTTGC
TCAGGACGTCATCAGCACCATCGGGCAGGCCTTTGAGTTGCGCTTCAAACAGTATCTCAGGAATCCACCC
AAGCTGGTCACCCCCCATGACAGGATGGCTGGCTTTGATGGCTCAGCTTGGGATGAGGAGGAAGAAGAGC
CCCCTGACCATCAGTACTACAATGACTTCCCAGGGAAGGAACCCCCTCTTGGTGGAGTGGTAGATATGAG
GCTTCGGGAAGGGGCTGCTCGACCCACTCTGCCCAGTACCCAGATGCCCAGCCACTTGGGAGCTACACTG
CCAATAGGGCAGCATGTTACAGGAGACCATGAAGTCCGTAAACAGATGCTGCCTCCACCACCGTGCCCAG
GCAGAGAACTCTTCGATGATCCTTCCTATGTCAACATCCAGAATCTAGACAAGGCCCGGCAAGCTGGTGG
TGGGGCTGGACCCCCAAATCCTTCTGTTAATGGCAGTGCACCCCGAGACCTCTTTGACATGAAGCCCTTC
GAAGATGCACTCCGGGTGCCGCCAGCTCCCCAGTCCATGTCCATGGCTGAGCAGCTCCAAGGGGAGTCCT
GGTTTCATGGGAAACTCAGCCGGCGGGAGGCTGAGGCGCTGCTGCAGCTCAACGGTGACTTCTTGGTGCG
AGAGAGTACAACCACACCTGGCCAGTACGTGCTCACTGGTCTGCAGAGTGGGCAGCCCAAGCACTTGCTT
CTGGTGGACCCTGAAGGTGTGGTTCGGACAAAGGATCACCGCTTTGAGAGTGTCAGTCACCTGATCAGCT
ACCACATGGACAATCACTTACCCATCATCTCTGCAGGCAGTGAGCTGTGTCTACAGCAACCTGTGGATCG
AAAAGTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_053517
Insert Size 1410 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053517.2, NP_445969.2
RefSeq Size 3108 bp
RefSeq ORF 1410 bp
Locus ID 85385
UniProt ID Q5M824
Gene Summary mouse homolog interacts with growth factor receptors; involved in transformation and differentiation in a Ras-dependent fashion [RGD, Feb 2006]
Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and uses a downstream start codon, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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