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Arpc1b (NM_019289) Rat Untagged Clone
CAT＃: RN201798
Arpc1b (untagged ORF) - Rat actin related protein 2/3 complex, subunit 1B (Arpc1b), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201798 representing NM_019289
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTACCACAGCTTCCTGGTGGAACCCATCAGCTGCCATGCCTGGAACAAGGACCGTACTCAGATCG CCATCTGCCCCAACAACCACGAGGTGCACATCTACGAGAAGAGCGGTGCCAAGTGGAACAAGGTGCACGA GCTCAAGGAGCACAACGGGCAGGTGACAGGCATCGACTGGGCCCCCGAGAGTAACCGCATTGTGACCTGC GGCACAGACCGCAATGCCTATGTGTGGACGCTGAAGGGCCGCACGTGGAAGCCCACGCTGGTCATCCTTC GGATTAATCGAGCTGCCCGCTGCGTGCGCTGGGCCCCCAATGAGAACAAGTTCGCCGTGGGCAGTGGCTC CCGTGTCATTTCCATCTGTTATTTTGAGCAGGAGAATGACTGGTGGGTGTGCAAACACATCAAAAAGCCC ATCCGCTCCACTGTCCTTAGCCTGGACTGGCACCCCAACAACGTGCTCCTGGCTGCAGGCTCCTGTGACT TCAAGTGCAGGATCTTCTCTGCCTATATCAAGGAGGTGGAGGAACGGCCAGCCCCTACACCGTGGGGCTC CAAGATGCCCTTTGGGGAGCTGATGTTTGAATCGAGCAGCAGCTGTGGCTGGGTGCATGGTGTCTGCTTC TCGGCCGGTGGGAGCCGAGTTGCTTGGGTCAGCCATGACAGCACTGTGTGCCTGGTAGATGCTGAGAAGA AGATGGCCGTGGCAACCCTGGCCTCTGAGACATTACCGCTCCTGGCCATCACCTTCATCACAGAAAATAG TCTCGTGGCAGCGGGCCACGACTGCTTCCCGGTGCTGTTTACCTATGACAACGCTGCGGGGACATTGAGC TTTGGTGGCCGGCTGGATGTGCCCAAGCAGAACTCCCAGCGTGGCCTGACAGCCCGAGAGCGCTTCCAGA ACCTCGACAAGAAGGCCAGCTCTGAAGGGGGTGCAGCCACAGGGGCTGGCCTGGATTCACTGCACAAGAA CAGCGTCAGTCAAATCTCGGTGCTCAGCGGGGGCAAGGCCAAGTGCTCGCAGTTCTGCACCACAGGCATG GACGGTGGCATGAGCATCTGGGATGTGAAGAGCTTGGAGTCAGCCTTGAAGGACCTGAAGATCAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019289 |
| Insert Size | 1119 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019289.2, NP_062162.1 |
| RefSeq Size | 1536 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 54227 |
| UniProt ID | O88656 |
| Gene Summary | Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201798 | Arpc1b (Myc-DDK-tagged ORF) - Rat actin related protein 2/3 complex, subunit 1B (Arpc1b), (10 ug) |
CNY 3,990.00 |
|
| RR201798L3 | Lenti ORF clone of Arpc1b (Myc-DDK-tagged ORF) - Rat actin related protein 2/3 complex, subunit 1B (Arpc1b), (10 ug) |
CNY 6,080.00 |
|
| RR201798L4 | Lenti ORF clone of Arpc1b (mGFP-tagged ORF) - Rat actin related protein 2/3 complex, subunit 1B (Arpc1b), (10 ug) |
CNY 6,650.00 |
