Grp (NM_133570) Rat Untagged Clone

CAT#: RN201864

Grp (untagged ORF) - Rat gastrin releasing peptide (Grp), (10 ug)



  "NM_133570" in other vectors (3)

CNY 1,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN201864 representing NM_133570
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCGGCTCCGAGCTCTCGCTCTTGCTGTTGGCTCTGGTCCTCTGCCAGGCTCCCCGGGGGCCAGCTG
CCCCGGTGTCGACAGGCGCAGGTGGAGGCACAGTCCTGGCCAAGATGTATCCGCGCGGCAGCCACTGGGC
TGTAGGACACTTAATGGGGAAGAAGAGCACAGATGAGTTGCCGCCCCTGTATGCAGCTGACAGAGATGGC
CTGAAGGAGCAACTGAGGGGATACATCCGCTGGGAAGAAGCTGCAAGGAATTTGCTTGGCCTCCTCGAAG
CCGCGGGGAACAGAAGCCACCAGCCACCTCAGGATCAGCCTCTGGGCAGTCTCCAGCCTACTTGGGATCC
AGAGGATGGCAGCTACTTCAGTGATGCTCAAAATGCTAAGTTGGTAGACTCTCTGCTCCAGGTTCTCAAG
GGGAAGGAAGGAACTGCCAGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_133570
Insert Size 444 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133570.5, NP_598254.2
RefSeq Size 883 bp
RefSeq ORF 444 bp
Locus ID 171101
UniProt ID P24393
Gene Summary increases 5-HT neuronal activity in the hypothalamus PVN; inhibits feeding behavior [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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