Shank3 (NM_021676) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Prosap2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201936 representing NM_021676 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGCCCCGGGGCCAGCGCCGTGGTCGTGCGCGTCGGCATCCCGGACCTGCAACAAACGAAGTGCC TGCGTCTGGATCCAACCGCGCCCGTGTGGGCCGCCAAGCAGCGTGTGCTCTGCGCCCTCAACCACAGCCT TCAGGACGCGCTCAACTACGGGCTATTCCAGCCTCCCTCCCGGGGTCGCGCCGGCAAGTTCCTGGATGAA GAGCGGCTCTTACAGGACTACCCGCCTAACCTGGACACGCCCCTGCCCTATCTGGAGTTTCGATACAAGC GGAGAGTTTATGCCCAGAACCTCATAGATGACAAGCAGTTTGCAAAGCTGCACACAAAGGCAAACCTGAA GAAGTTCATGGACTATGTCCAGCTACACAGCACAGACAAGGTGGCCCGCCTGCTGGACAAGGGGCTGGAC CCCAATTTCCATGACCCTGACTCAGGAGAGTGCCCTCTGAGCCTTGCAGCACAGTTGGACAACGCCACTG ACCTCCTGAAGGTTCTTCGCAATGGCGGTGCTCATCTGGACTTCCGAACCCGAGATGGGCTAACCGCTGT CCACTGCGCCACCCGACAGCGGAATGCGGGAGCATTGACGACCCTGCTGGACCTGGGGGCTTCACCTGAC TACAAGGACAGCCGCGGCCTGACGCCCCTGTACCATAGTGCCCTAGGGGGCGGGGATGCCCTCTGCTGTG AGCTGCTTCTCCATGATCACGCACAGTTGGGGACCACTGACGAGAATGGCTGGCAGGAGATCCATCAGGC CTGTCGCTTTGGGCATGTACAGCACTTGGAGCACCTGCTGTTCTATGGGGCCAACATGGGTGCCCAGAAC GCCTCGGGAAACACAGCCTTGCACATCTGTGCCCTCTATAACCAGGAGAGCTGTGCCCGCGTCCTGCTTT TCCGTGGTGCCAACAAGGACGTCCGCAATTACAACAGCCAGACAGCCTTCCAGGTGGCCATTATTGCAGG GAACTTTGAGCTTGCCGAGGTAATCAAGACCCACAAAGACTCGGATGTCGTACCATTCAGGGAAACCCCC AGCTATGCAAAGCGACGACGTCTGGCTGGCCCGAGTGGCTTGGCATCCCCTCGGCCCTTACAGCGCTCAG CCAGTGATATCAACCTGAAGGGTGACCAGCCCGCAGCTTCTCCCGGGCCCACTCTCCGAAGCCTCCCTCA CCAACTGCTGCTCCAGAGGCTTCAGGAGGAGAAAGACCGGGACAGGGATGGTGAGCAGGAGAACGACATC AGCGGTCCCTCAGCAGGCAGGGGCGGCCACAGCAAGATCAGCCCCAGCGGGCCCGGCGGATCCGGCCCCG CGCCCGGCCCCGGCCCGGCGTCTCCCGCGCCCCCCGCGCCGCCGCCCCGGGGCCCGAAGCGGAAACTTTA CAGTGCCGTCCCCGGCCGCAAGTTCATCGCTGTGAAGGCGCACAGCCCGCAGGGCGAGGGCGAGATCCCG CTGCACCGCGGCGAGGCCGTGAAGGTGCTCAGCATTGGGGAGGGCGGTTTCTGGGAGGGAACCGTGAAGG GCCGTACAGGCTGGTTCCCAGCTGACTGTGTGGAGGAAGTGCAGATGCGACAGTATGACACACGGCATGA AACTCGAGAGGACCGGACGAAGCGTCTTTTCCGCCACTACACTGTGGGTTCCTATGACAGCCTCACTTCA CACAGTGATTATGTCATTGATGATAAGGTGGCTATCCTGCAAAAACGGGACCATGAGGGTTTTGGCTTTG TTCTCCGGGGAGCCAAAGCAGAGACCCCCATTGAGGAGTTTACACCCACACCTGCCTTCCCTGCGCTCCA GTACCTTGAGTCTGTAGATGTGGAAGGTGTGGCCTGGAAGGCTGGGCTTCGCACTGGGGACTTCCTCATT GAGGTAAACGGAGTGAACGTCGTGAAGGTTGGACACAAGCAAGTGGTGGGTCTCATCCGTCAGGGTGGCA ACCGTCTGGTCATGAAGGTTGTGTCTGTTACCAGGAAGCCAGAGGAGGATAGTGCTCGGCGCAGAGCCCC ACCACCTCCCAAGAGGGCCCCCAGCACCACGCTGACCCTGCGGTCCAAGTCCATGACGGCTGAGCTCGAG GAACTCGCTTCCATTCGGAGAAGGAAAGGGGAGAAGTTGGATGAGATCCTGGCGGTTGCTGCGGAACCAA CGCTGAGGCCAGACATTGCAGACGCTGATTCCAGGGCAGCCACTGTCAAGCAGCGGCCCACCAGCCGGAG GATTACCCCTGCCGAGATCAGCTCATTGTTTGAGCGACAGGGCCTCCCGGGCCCAGAGAAGCTGCCGGGC TCTCTGCGGAAGGGGATTCCACGGACCAAATCTGTAGGGGAGGATGAGAAGCTGGCATCCCTACTGGAAG GGCGTTTCCCACGCAGCACATCAATGCAAGACACAGTGCGTGAAGGCCGAGGCATTCCGCCCCCACCGCA GACCGCCCCGCCACCCCCACCCGCGCCCTACTACTTCGACTCCGGGCCACCCCCCACCTTCTCACCACCG CCACCACCACCGGGCCGGGCCTATGACACTGTGCGCTCCAGCTTCAAGCCAGGCCTGGAGGCTCGTCTGG GTGCAGGGGCAGCTGGCCTGTATGATTCTGGCACACCTCTGGGCCCGCTGCCCTACCCTGAGCGCCAGAA GCGTGCACGCTCCATGATCATATTGCAGGACTCTGCGCCAGAAGTGGGCGATGTACCCCGGCCTGCGCCT GCAGCCACACCGCCTGAGCGCCCCAAGCGCCGGCCTCGGCCGTCAGGCCCTGATAGTCCCTATGCCAACC TGGGCGCCTTCAGTGCCAGCCTCTTTGCTCCGTCGAAACCGCAGCGCCGCAAGAGTCCGCTGGTGAAGCA GCTTCAGGTGGAGGACGCTCAGGAGCGCGCGGCGTTGGCCGTGGGTAGCCCGGGACCAGTGGGTGGAAGC TTTGCACGAGAACCCTCCCCAACGCACCGCGGGCCCCGACCGGGCGGCCTTGACTACAGCTCTGGAGAAG GCCTGGGGCTCACCTTTGGCGGCCCTAGCCCTGGCCCAGTCAAGGAGCGGCGCCTGGAGGAGCGACGCCG TTCCACTGTGTTCCTGTCTGTGGGTGCCATCGAGGGCAACCCTCCCAGCGCGGATCTGCCATCCCTACAA CCCTCCCGCTCCATTGATGAGCGCCTCCTGGGGACAGGCGCCACCACTGGCCGAGATTTGCTGCTCCCCT CCCCTGTCTCTGCTCTGAAGCCATTGGTCGGTGGTCCCAACCTTGGGCCCTCAAGCTCCACCTTCATCCA TCCTCTTACTGGCAAACCCTTGGATCCTAGCTCACCCCTAGCTCTTGCTCTGGCTGCCCGAGAGCGGGCT CTGGCCTCGCAAACACCTTCCCGGTCCCCCACACCCGTGCACAGTCCTGATGCTGACCGCCCTGGACCCC TCTTTGTGGATGTGCAAACCCGAGACTCCGAGAGAGGACCCTTGGCCTCCCCAGCCTTCTCCCCTCGGAG TCCAGCCTGGATTCCAGTGCCTGCTCGCAGAGAGGCAGAGAAGCCCACTCGGGAAGAGCGGAAGTCACCA GAGGACAAGAAATCCATGATCCTCAGCGTCTTGGACACGTCCTTGCAACGGCCAGCTGGCCTCATTGTTG TGCATGCCACCAGCAATGGACAGGAGCCCAACAGGCTGGGGGCTGAAGAGGAGCGCCCGGGTACTCCGGA GCTGGCCCCAACCCCCATGCAGGCAGCAGCTGTGGCAGAGCCCATGCCAAGCCCACGAGCCCAACCCCCT GGCAACATCCCAGCAGATCCCGGGCCAAGCCAAGGCAACTCAGAGGAGGAGCCAAAGCTGGTATTCGCTG TGAACCTGCCACCTGCTCAACTGTCCTCCAACGATGAGGAGACCAGAGAGGAGCTGGCCCGCATTGGGCT AGTGCCACCCCCTGAAGAGTTTGCCAATGGGATCCTGCTGGCCACCCCACCCCCAGGACCGGGCCCCTTG CCCACCACGGTACCCAGCCCGGCCTCAGGGAAGCCCAGCAGCGAGCTGCCCCCTGCCCCGGAGTCTGCAG CTGACTCTGGAGTAGAGGAGGCCGACACTCGAAGCTCCAGTGACCCCCACCTGGAGACCACAAGCACCAT TTCCACAGTGTCCAGCATGTCCACCCTGAGCTCGGAGAGTGGAGAACTCACTGACACCCACACCTCCTTT GCCGATGGACACACTTTTCTACTCGAGAAGCCACCAGTGCCTCCCAAGCCCAAACTCAAGTCCCCGCTGG GGAAGGGGCCGGTGACCTTCAGGGGCCCGCTGCTGAAGCAATCCTCGGACAGTGAGCTCATGGCCCAGCA GCACCATGCCACCTCTACTGGGTTGACTTCTGCTGCTGGGCCTGCCCGCCCTCGCTACCTCTTCCAGAGA AGGTCCAAGCTGTGGGGGGACCCCGTGGAGAGTCGGGGGCTCCCTGGGCCTGAGGATGACAAACCAACTG TGATCAGTGAGCTCAGCTCCCGTCTGCAGCAGCTGAATAAAGACACTCGCTCCTTGGGGGAGGAACCAGT TGGTGGCCTGGGTAGCCTGCTGGACCCTGCTAAGAAGTCGCCCATTGCAGCAGCTCGCTGCGCGGTGGTC CCGAGTGCCGGCTGGCTCTTCAGCAGCCTCGGTGAGCTGAGCACCATCTCAGCGCAGCGCAGCCCCGGGG GCCCGGGCGGAGGGGCCTCCTACTCGGTGCGGCCCAGCGGCCGGTACCCCGTGGCGAGACGAGCCCCGAG CCCAGTGAAACCCGCATCGCTGGAGCGGGTGGAGGGGCTGGGGGCGGGCGTGGGAGGCGCGGGGCGGCCC TTCGGCCTCACGCCTCCCACCATCCTCAAGTCGTCCAGCCTCTCCATCCCGCACGAACCCAAGGAAGTGC GCTTCGTGGTGCGAAGTGCGAGTGCGCGCAGCCGCTCCCCCTCACCATCTCCGCTGCCCTCGCCTTCTCC TGGCTCTGGCCCCAGTGCCGGCCCGCGTCGGCCATTTCAACAGAAGCCCCTGCAGCTTTGGAGCAAGTTC GATGTGGGCGACTGGCTGGAGAGCATCCACTTAGGCGAGCACCGAGACCGCTTCGAGGACCATGAGATCG AAGGCGCACACCTGCCTGCGCTCACCAAGGAAGACTTCGTGGAGCTGGGAGTCACACGCGTTGGCCACCG CATGAACATCGAGCGTGCGCTCAGGCAGCTGGATGGCAGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_021676
Insert Size	5223 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021676.1, NP_067708.1
RefSeq Size	7391 bp
RefSeq ORF	5223 bp
Locus ID	59312
UniProt ID	Q9JLU4
Gene Summary	may be involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]

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