Hecw2 (NM_001108218) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202525 representing NM_001108218 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCTCAGCCCGGGAGCATTTGCTTTTTGTGCGGCGTCGAAATCCACAGATGCGGTACACACTGA GCCCAGAGAACCTTCAGAGCCTCGCAACCCAGAACTCAATGCCCGAGAACATGGCCCTGCAGCGAGCCAA CAGTGACACCGACCTCGTGACCTCCGAGAGCCGCTCCAGCCTGACTGCCAGCATGTATGAGTACACACTG GGGCAAGCCCAGAACCTCATTATCTTCTGGGACATTAAGGAAGAGGTTGACCCCAGCGATTGGATCGGAC TCTATCACATAGACGAGAATTCTCCAGCCAACTTCTGGGATTCTAAAAACAGGGGCGTGACTGGAACCCA AAAAGGACAAATTGTATGGCGGATTGAGCCCGGGCCCTACTTCATGGAACCGGAGATCAAAATCTGTTTC AAATACTACCATGGCATTAGCGGAGCCCTGCGAGCCACAACCCCCTGTATTACTGTGAAGAACCCGGCTG TGATGATGGGTGCAGAAGGCATGGAAGGATGTGCTTCAGGAAGCCTGCACTCTAGGAAACTCGTCAGCTT CACATTGTCAGATCTCAGGGCAGTTGGGCTCAAGAAAGGGATGTTCTTCAATCCTGACCCTTATCTTAAG ATGTCCATTCAGCCAGGGAAGAAGAGCAGTTTCCCTACCTGTGCCCACCACGGGCAGGAGAGAAGATCCA CTATCATCAGTAACACCACCAATCCAATCTGGCACAGAGAGAAATACTCCTTTTTTGCACTTTTGACCGA TGTCTTAGAAATTGAAATCAAAGACAAATTTGCCAAGAGCCGGCCCATCATCAAACGCTTCCTGGGAAAA TTAACCATTCCAGTGCAGAGGCTGTTGGAGAGGCAGGCTGTCGGTGACCAGATGCTCAGCTACAACCTTG GCAGAAGGCTCCCCGCTGATCACGTGAGTGGCTACCTCCAGTTTAGAGTGGAAGTCCCATCCTCTGCCCA GGAAGATGCATCTCCAGAGGCCGTTGGCACCATCCTCGGTGTCCACACTGTGAATGGAGACCTGGGCAGC CCTTCTGATGATGAGGACATGCCAGGGAGCCATCATGACAGCACCATTTGTGCTAACGGGCCAGTGTCTG AGGACAGTGTTGCTGACGGAACCCCCAAGCATTCTTTCAGGACTAGCTCTACCCTGGAGATTGACACAGA GGACTTGATCTCTACTTCTTCAAGAAATTCACCCCCCAGGGGCCGCCAGGATTCACTCAATGATTACTTA GATGCTATTGAACACAATGGCCCCTCTAGGCCAGGGACAGCCTCCTCCTCTGAGCGGCCCATGGGAGCCT CTCCCAAACTGAGGAGTAGTTTTCCCACTGACACCAGACTCAATGCGATGCTTCATATAGACTCAGATGA AGAAGACCATGATTTTCAGCAAGACCTAGGCTACCCATCCTCCTTGGAGGAGGAAGGAGGCCTGATCATG TGCAGCAGGACATCGAGGATCGATGATGGGAGCCTGACGTCTCAGACCAAACCAGAGGATGACAACCCTG TTGAGAACGAGGATGCCTCCGTACATGAAACTGCTTCCTTAGAGGAGAGGCCAGAGAATCTTCCCGAGGT TGAAGATGGCTCCTTGCCGTCAGGTACAGCCCCTGATGAAAATGAAGCCAGCGTAGAGCCTCAGCCCAGT GCTGACCAGGGCAGCACTGAATTGTGTAGCTCTCAAGAAGTCGATCAGCCCACAAGTGGCACGGACGCAG GAGCTTCTGACACATCTGTAGGAAGCCGCAGAGCTGCCAGTGAGACTGAGTCCCTGGACCAGGGATCCGA ACCTTCCCAGGTGTCCTCTGAGACAGAACCCAGTGACCCTGCAAGGACAGAGAGTGTGAGTGAGGCCAGT ACCAGACCCGAGGGAGAGAGTGACCTGGAAGGTGCAGACAGCTCATGCAATGAGAGCGTGACCACACAGC TGTCCTCCGTGGAGACACGGTGCTCATCTCTAGAGAGTGCACGATTTCCAGAAACCCCGGCCTTTTCTTC TCAGGAAGAGGAAGATGGGGCCTGCGCAGCTGAGCCGACCAGCAGCGGCCCCACAGAGGGGTCGCAGGAG TCTGTCTGCACTCCCGGTTCTTTACCTGCTGTGCAGGTGCCCAGCAGGGAAGAGGAAGGGTCGGGGGCTG AAGCTACTGCTTTATCTGAGCAGGGGGAGCTGGGGGAGGTCTGGCAGAGGAGGGGCAGTCTTGAGGGAGC AGCTGCTGCTGCCACTGCTGCTGCCACCGATAGCCAGCCCCAGGAAGACGGGGATCCTGGTGATACACAA GGTGCCTGCGAAGGGGCCACCGCTCAGGAGGAGGGCGCCACTGGAGGTTCTCAAACCAATGGCCATCAGC CCTTGAGGTCACTACCTTCAGTGCGCCAGGACGTCAGCCGATACCAGAGGGTGGACGAGGCTCTCCCACC AAACTGGGAAGCACGTATTGACAGCCACGGCAGAATCTTCTACGTGGACCATGTAAACAGAACCACGACG TGGCAGCGGCCTACAGCTCCCCCTGCCCCACAGGTCCTGCAGAGATCTAACTCCATACAGCAAATGGAGC AGCTAAACCGGCGTTATCAGAGCATCCGCAGAACCATGACGAATGAGAGGCCTGAGGAAAATACCAGTGC TATCGATGGGGCAGGGGAAGAAGCCGACTTTCACCAAGCCAGCGCAGACTTCCGTCGGGAGAACGTCCTG CCCCACTCTACCTCCAGATCCAGGCTCACGCTGCTGTTACAGTCGCCCCCTGTGAAGTTCCTCATCAGCC CAGAGTTCTTCACCGTGCTGCATTCTAACCCTAGTGCCTACCGCATGTTTACAAACAACACGTGTTTGAA GCACATGATCACCAAAGTCCGGCGGGACACCCACCACTTTGAACGCTATCAGCATAACAGGGACCTCGTG GGATTCCTCAACATGTTTGCTAACAAACAGCTGGAACTACCAAGGGGCTGGGAGATGAAACACGATCATC AGGGCAAGGCATTTTTTGTCGACCACAACTCCCGAACCACCACGTTCATTGATCCTCGGCTCCCACTTCA GAGCAGTAGGCCCACGAGTGCGCTGGTTCATCGACAACACCTGACGAGGCAACGCAGTCACAGCGCGGGT GAGGTAGGAGAAGACTCTCGGCATGCAGGACCACCCGTTCTTCCCAGGCCAGCCAGTACATTCAATACGG TCAGTAGGCCACAGTACCAGGACATGGTTCCAGTGGCCTACAATGACAAAATTGTTGCGTTTTTGCGTCA ACCTAACATCCTTGAAATTCTGCAAGAGCGTCAACCTGATCTTGCCAGAAACCACTCACTCAGGGAGAAG ATCCAATTTATCCGAACTGAAGGGACCCCTGGATTGGTGCGGCTCTCAAGTGATGCAGATCTTGTCATGT TACTGAGCTTATTTGAGGAAGAAATAATGTCATATGTGCCTCCTCATGCCTTACTCCACCCCAGCTACTG TCAGTCCCCGCGTGGCTCCCCTGTGTCATCTCCCCAGAATTCACCAGGTACTCAGCGTGCCAATGCCCGG GCTCCTGCACCTTACAAGAGAGATTTTGAAGCCAAACTAAGGAACTTTTATAGGAAGTTAGAAACTAAAG GATACGGACAAGGCCCAGGAAAATTAAAGTTAATTATCCGAAGAGACCACTTGCTGGAAGATGCTTTTAA TCAGATTATGGGCTACTCCAGAAAGGACCTGCAGAGGAATAAACTGTACGTCACCTTTGTCGGGGAGGAA GGGTTGGATTACAGTGGGCCTTCCAGAGAGTTTTTCTTCCTGGTATCCCGAGAACTCTTTAACCCCTATT ACGGCTTATTTGAATATTCAGCCAATGACACATACACAGTACAAATAAGTCCTATGTCTGCCTTTGTAGA CAATCATCATGAATGGTTTCGATTCAGTGGGAGGATCCTCGGTCTTGCACTAATACACCAGTACTTGTTG GACGCCTTCTTTACGCGACCCTTCTATAAGGCTCTTCTCAGAATCCTGTGTGACCTGAGTGATCTGGAAT ACCTTGATGAGGAGTTCCACCAGAGCCTTCAATGGATGAAAGACAATGACATCCATGACATCCTAGACCT CACGTTCACTGTGAACGAAGAAGTTTTTGGGCAGATTACTGAACGAGAGTTAAAACCAGGGGGAGCCAAT ATTCCAGTGACGGAGAAGAACAAGAAGGAGTACATTGAGAGGATGGTGAAGTGGAGGATTGAGAGGGGCG TGGTGCAGCAGACAGAGAGCTTAGTCCGCGGTTTCTATGAGGTAGTAGATGCCAGGCTAGTGTCTGTCTT TGATGCAAGAGAACTGGAGCTGGTCATTGCTGGCACAGCTGAGATAGATCTCAATGACTGGAGGAACAAC ACTGAATACAGAGGCGGGTACCATGACAATCACATTGTAATTCGGTGGTTCTGGGCTGCAGTAGAACGAT TCAACAATGAACAACGACTAAGGTTGTTACAGTTTGTTACAGGCACATCTAGCATTCCCTATGAAGGATT TGCTTCTCTCCGAGGGAGTAATGGTCCAAGAAGATTCTGTGTGGAAAAATGGGGAAAAATCACCGCTCTT CCCAGAGCTCACACGTGTTTTAACCGCCTGGATCTCCCTCCATACCCTTCCTTTTCCATGCTTTATGAAA AACTCTTGACAGCAGTTGAAGAGACCAGTACCTTCGGACTCGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108218
Insert Size	4737 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108218.1, NP_001101688.1
RefSeq Size	5296 bp
RefSeq ORF	4737 bp
Locus ID	316395

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