Fbn1 (NM_031825) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202569 representing NM_031825 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCGAGGAGGGCTGCTGGAGGTCGCGCTGGCTTTCGCCCTGCTCCTCGAGTCCTACACGAGCCATG GGGCGGATGCCAATCTGGAGGCTGGGAGCCTAAAGGAGACCAGAGCCAATCGCGCCAAGAGACGAGGCGG CGGAGGACACGATGCTCTGAAAGGACCCAATGTCTGTGGATCTCGTTATAACGCATACTGTTGTCCCGGA TGGAAAACCTTACCTGGTGGAAATCAGTGTATTGTTCCCATTTGCCGGCATTCCTGTGGGGACGGATTCT GCTCAAGACCAAACATGTGCACTTGCCCGTCTGGTCAGATATCGCCTTCCTGTGGCTCCAGATCCATACA ACACTGTAGCATCCGCTGTATGAATGGGGGCAGCTGCAGCGATGACCACTGTCTGTGCCAGAAGGGGTAC ATTGGCACTCACTGCGGACAACCTGTCTGTGAAAGTGGCTGTCTCAATGGAGGGAGGTGCGTGGCCCCAA ATCGGTGTGCATGCACATACGGCTTTACGGGACCCCAGTGTGAAAGAGATTACAGAACAGGCCCGTGTTT TACTGTGGTAAGCAACCAGATGTGCCAGGGACAGCTCAGCGGGATTGTCTGCACCAAAACACTCTGCTGT GCCACCGTGGGCCGAGCCTGGGGCCACCCCTGCGAGATGTGTCCTGCCCAGCCTCACCCCTGCCGCCGCG GCTTCATTCCCAACATCCGCACTGGAGCTTGTCAAGATGTGGATGAATGTCAGGCTATCCCAGGGCTGTG TCAAGGAGGAAATTGCATTAATACCGTTGGATCTTTTGAGTGCAAATGCCCCGCTGGACACAAATTTAAT GAAGTGTCCCAAAAATGTGAAGATATCGATGAGTGCAGCACCATCCCTGGTGTGTGCGATGGCGGGGAGT GTACCAACACGGTCAGCAGTTATTTCTGCAAATGTCCCCCTGGCTTTTACACCTCTCCCGATGGCACCAG ATGCGTAGATGTTCGCCCTGGTTACTGCTACACAGCTCTGACAAATGGGCGCTGCTCTAACCAGCTGCCA CAGTCCATAACCAAAATGCAGTGCTGTTGTGATGTTGGCCGGTGCTGGTCTCCAGGGGTTACTGTCGCTC CTGAGATGTGTCCCATCAGGTCAACTGAGGACTTCAACAAGCTGTGCTCCGTCCCTCTGGTAATCCCCGG GAGACCAGACTATCCTCCCCCACCCCTCGGCCCCCTTCCTCCGGTTCAGCCTGTTCCTGGGTTTCCTTCC GGGCCTGTGATACCGGTCCCCCGGCCACCGCCAGAATACCCGTATCCATCTCCATCACGGGAACCACCAA AGGTGCTGCCTTTCAACGTTACTGACTACTGTCAATTGGTCCGCTATCTCTGTCAAAATGGGCGCTGCAT TCCAACTCCCGGTAGCTACCGCTGTGAGTGCAACAAGGGCTTCCAGCTGGATATCCGTGGCGAGTGCATC GACGTTGATGAGTGTGAGAAAAACCCATGCACCGGTGGAGAATGTATCAACAACCAGGGCTCATATACCT GTCACTGCCGAGCTGGCTACCAGAGCACACTCACCAGGACTGAGTGCAGAGACATAGATGAGTGTCTGCA GAACGGCCGGATCTGTAACAACGGGCGCTGCATCAACACAGATGGCAGCTTCCACTGCGTGTGCAATGCG GGCTTTCACGTCACGAGGGACGGAAAGAACTGTGAAGACATGGATGAGTGCAGCATCAGGAACATGTGCC TGAATGGGATGTGCATCAACGAAGATGGGAGTTTCAAGTGTATTTGCAAACCTGGGTTCCAACTGGCGTC AGATGGCCGCTACTGCAAAGATATCAATGAGTGTGAGACTCCTGGAATCTGCATGAACGGACGATGCGTG AACACTGATGGCTCCTACAGATGCGAATGCTTTCCCGGATTGGCTGTGGGCCTAGATGGACGCGTGTGTG TTGACACACACATGCGGAGCACATGCTACGGAGGCTACAGGAGAGGCCAGTGTGTGAAGCCGTTGTTTGG TGCTGTTACCAAATCGGAATGCTGTTGTGCCAGCACCGAGTATGCCTTTGGGGAAGCCTGCCAGCCGTGT CCTGCACAGAATTCAGCGGAATATCAGGCACTTTGCAGCAGTGGACCCGGAATGACATCAGCAGGCTCTG ATATAAATGAATGTGCATTAGATCCTGACATTTGCCCAAATGGAATTTGTGAAAATCTCCGTGGGACCTA CAAATGTATATGCAACTCGGGATACGAAGTAGACATAACTGGGAAAAACTGTGTCGATATTAATGAGTGT GTGCTGAACAGTCTACTTTGTGACAATGGACAATGTCGAAACACACCTGGAAGTTTTGTCTGTACCTGCC CCAAGGGATTTGTGTACAAACCTGACTTAAAGACCTGTGAAGACATTGATGAATGTGAATCGAGTCCTTG CATTAATGGAGTCTGCAAGAACAGCCCTGGGTCTTTCATTTGCGAATGCTCTCCTGAAAGTACTCTGGAT CCAACAAAAACCATCTGCATAGAAACCATCAAGGGCACTTGCTGGCAGACTGTCATTGACGGGCGATGTG AGATCAATATCAATGGAGCCACCTTGAAGTCCGAGTGCTGCTCCTCCCTTGGCGCTGCCTGGGGGAGCCC GTGCACTATATGTCAAGTTGATCCCATATGTGGTAAAGGGTACTCGAGAATTAAAGGCACGCAATGTGAA GATATCAATGAGTGTGAAGTATTCCCGGGAGTATGCAAGAATGGCCTGTGTGTTAACTCCAGGGGTTCCT TCAAGTGCGAGTGTCCCAGTGGAATGACTTTGGATGCTACAGGAAGAATCTGTCTTGATATCCGCCTGGA GACCTGCTTCCTCAAGTACGACGACGAGGAGTGCACGCTGCCCATTGCTGGCCGCCACCGAATGGATGCC TGCTGCAGCTCCGTTGGGGCAGCCTGGGGAACAGAAGAGTGTGAGGAGTGTCCATTGAGAAACAGCCGGG AGTATGAGGAACTCTGTCCCCGAGGACCCGGGTTTGCCACGAAAGACATAACAAATGGGAAACCTTTCTT CAAAGACATTAATGAGTGCAAGATGATACCCAGCCTCTGTACCCACGGCAAGTGTAGGAACACCATTGGC AGCTTCAAGTGCAGGTGTGACAGTGGCTTTGCTTTGGACTCTGAAGAGAGGAACTGCACAGACATTGACG AGTGCCGCATATCTCCTGACCTCTGTGGCCGAGGCCAGTGTGTGAACACCCCGGGGGATTTCGAATGCAA GTGTGACGAAGGCTATGAAAGTGGCTTCATGATGATGAAGAACTGTATGGATATTGATGAATGCCAGAGA GATCCTCTCCTCTGTCGAGGAGGCATCTGCCACAACACAGAGGGAAGTTATCGCTGTGAATGTCCTTCTG GCCACCAACTGTCTCCAAACATCTCTGCGTGCATTGACATCAACGAGTGTGAACTGAGCGCGAACCTCTG TCCCCACGGGCGTTGTGTGAACCTCATTGGGAAGTACGAGTGTGCCTGCAACCCGGGCTATCACCCCACT CATGATAGGCTTTTCTGTGTCGACATTGATGAATGCAGCATAATGAATGGTGGTTGTGAGACCTTCTGCA CAAACTCTGACGGGAGCTATGAATGTAGCTGTCAGCCAGGCTTCGCGCTGATGCCAGACCAGCGATCGTG CACAGACATTGATGAGTGTGAAGATAACCCCAATATCTGTGATGGTGGCCAGTGCACAAACATTCCTGGA GAGTACAGGTGCTTGTGCTATGATGGGTTCATGGCGTCTGAAGACATGAAGACTTGTGTTGATGTCAATG AATGTGACCTGAATCCCAACATCTGCCTCAGTGGGACCTGCGAGAACACTAAAGGCTCCTTCATCTGCCA CTGTGATATGGGCTACTCAGGGAAAAAAGGAAAAACTGGCTGCACAGATATCAATGAATGTGAGATTGGA GCACACAACTGTGGCAGACATGCTGTGTGCACAAATACAGCAGGGAGCTTCAAGTGCAGCTGCAGTCCTG GCTGGATTGGAGATGGCATTAAGTGCACAGATCTGGATGAATGCTCTAATGGAACCCACATGTGCAGCCA ACACGCAGACTGCAAGAACACCATGGGGTCATATCGCTGTCTCTGTAAGGATGGCTACACAGGGGATGGC TTCACCTGCACAGACCTTGACGAGTGCTCTGAGAACCTGAACCTCTCTGGCAATGGCCAGTGCCTCAATG CCCCTGCGGGGTACCGCTGTGAATGCGACATGGGCTTCGTGCCCAGTGCTGATGGGAAGGCCTGTGAAGA TATCAACGAGTGCTCCCTTCCAAACATCTGTGTCTTTGGAACTTGCCACAATCTCCCGGGCCTCTTCCGC TGCGAGTGTGAGATTGGCTACGAGCTGGACCGAAGTGGTGGAAACTGCACAGATGTTAATGAGTGTCTGG ATCCTACCACCTGCATCAGTGGGAACTGTGTCAACACTCCAGGCAGTTACACATGCGACTGTCCACCTGA TTTTGAGCTGAATCCAACTCGAGTGGGCTGTGTCGATACTCGCTCTGGAAACTGCTATCTGGATATCCGA CCCCGGGGAGACAATGGAGATACGGCCTGCAGAAATGAAATTGGAGTTGGTGTTTCTAAGGCTTCCTGCT GTTGCTCTCTGGGTAAAGCTTGGGGAACCCCATGTGAGCTGTGTCCTCCTGTGAACACATCCGAGTATAA AATTCTCTGCCCCGGAGGAGAAGGTTTTCGTCCAAATCCCATCACCGTTATATTGGAAGACATCGATGAG TGCCAGGAGCTCCCAGGGCTGTGCCAAGGGGGGAAGTGCATCAACACCTTTGGCAGCTTCCAGTGTCGCT GTCCAACGGGTTACTACCTGAATGAAGACACACGGGTGTGTGATGATGTGAACGAATGTGAGACTCCCGG AATCTGTGGTCCGGGGACATGTTACAACACCGTTGGCAACTATACCTGCATTTGTCCTCCAGACTACATG CAAGTGAATGGGGGGAATAATTGCATGGACATGAGAAGAAGCCTGTGCTACAGAAACTATCATGCTGACA ACCAGACCTGCGATGGAGAACTGCTCTTCAACATGACCAAGAAGATGTGTTGCTGCTCCTACAACATCGG CCGTGCCTGGAATAAGCCCTGTGAACAGTGTCCCATCCCAAGCACAGATGAATTTGCTACCCTCTGTGGA AGCCAGAGGCCAGGCTTCGTGATTGACATTTACACAGGTTTACCTGTGGATATTGATGAATGCCGGGAGA TCCCAGGGGTTTGTGAAAATGGAGTGTGCATCAACATGGTTGGCAGCTTCCGGTGTGAGTGCCCTGTGGG ATTCTTCTACAACGACAAGTTGCTGGTTTGTGAAGATATCGATGAGTGTCAGAATGGCCCCGTGTGCCAG CGCAATGCGGAATGCATCAACACCGCAGGCAGCTACCGCTGTGACTGCAAACCCGGCTACCGCCTCACCT CCACAGGTCAATGCAACGATCGAAACGAGTGCCAAGAAATCCCGAACATATGCAGTCATGGCCAGGTCAT CGACACTGTGGGAAGCTTCTACTGCCTCTGTCACACAGGCTTCAAAACAAACATAGATCAGACCATGTGC TTAGACATAAATGAGTGTGAGAGAGACGCCTGTGGGAATGGGACTTGCAGAAACACAATTGGTTCCTTCA ACTGCCGCTGCAACCATGGCTTCATACTCTCTCACAACAATGACTGCATAGACGTTGATGAATGTGCAAC TGGAAACGGGAACCTTTGTAGAAATGGCCAGTGCGTCAACACGGTGGGGTCCTTTCAGTGCAGGTGCAAT GAAGGCTACGAGGTGGCTCCAGACGGCAGGACCTGTGTGGATATCAATGAATGTGTTCTGGATCCTGGGA AATGTGCACCTGGAACCTGTCAGAACTTGGACGGCTCCTACAGATGCATTTGCCCACCTGGGTACAGTCT ACAGAATGACAAATGTGAAGATATTGATGAGTGTGTCGAAGAGCCAGAAATCTGTGCTTTGGGGACCTGC AGCAACACTGAAGGCAGCTTCAAATGTCTGTGTCCAGAAGGGTTCTCCCTGTCCTCTACTGGAAGAAGGT GCCAAGACTTGAGAATGAGCTACTGCTACGCGAAGTTTGAAGGTGGGAAGTGTTCGTCGCCCAAATCCAG GAATCATTCCAAGCAGGAATGCTGCTGCGCTTTGAAGGGAGAAGGCTGGGGAGACCCTTGTGAGTTGTGT CCCACTGAGCCAGATGAGGCTTTCCGCCAGATATGCCCCTTTGGAAGTGGGATCATCGTGGGCCCTGATG ACTCGGCAGTTGATATGGATGAATGCAAAGAACCTGATGTCTGTAAACATGGGCAGTGCATCAACACAGA CGGCTCCTATCGCTGCGAGTGTCCCTTTGGTTATATTCTAGAAGGGAATGAGTGTGTGGATACCGATGAA TGCTCCGTGGGCAATCCTTGTGGAGATGGGACCTGCAAGAATGTGATTGGAGGTTTTGAATGTACCTGTG AGGAGGGATTCGAGCCTGGTCCAATGATGACATGTGAAGATATAAATGAATGTGCCCAGAATCCCCTACT CTGCGCCTTCCGCTGTGTAAATACCTATGGGTCCTATGAATGCAAGTGCCCTGTTGGATATGTTCTGCGA GAAGACAGGAGGATGTGTAAAGATGAGGATGAGTGTGCAGAGGGGAAGCACGACTGTACTGAAAAGCAAA TGGAGTGCAAGAACCTCATCGGTACCTACATGTGCATCTGCGGCCCAGGGTACCAGCGAAGACCTGATGG AGAGGGCTGCATAGATGAGAATGAATGTCAGACCAAGCCTGGGATCTGTGAGAACGGGCGTTGCCTCAAC ACCCTGGGGAGCTACACCTGTGAGTGTAATGATGGCTTCACAGCCAGCCCCACTCAGGATGAGTGCTTGG ACAACCGGGAAGGGTACTGCTTTTCTGAGGTACTGCAGAACATGTGCCAGATTGGATCAAGCAACAGAAA CCCCGTCACCAAGTCCGAGTGCTGCTGTGATGGAGGGAGAGGCTGGGGACCCCACTGTGAGATCTGCCCC TTCGAGGGCACAGTGGCTTACAAGAAACTGTGTCCCCACGGCCGAGGATTCATGACCAACGGAGCAGATA TTGATGAGTGCAAGGTAATCCACGATGATTGCCGAAACGGGGAGTGTGTCAATGACAGAGGATCATATCA CTGCATCTGTAAAACTGGCTACACTCCGGACATAACAGGGACCGCCTGTGTAGATCTAAATGAGTGCAAC CAGGCTCCCAAACCCTGCAATTTCATATGCAAAAACACAGAAGGGAGTTACCAGTGTTCCTGCCCCAAGG GCTATATTCTGCAAGAGGATGGAAGGAGCTGCAAAGATCTTGACGAGTGTGCAACCAAGCAGCATAACTG TCAGTTCCTGTGTGTTAACACCATCGGCGGCTTCACATGCAAATGCCCTCCTGGGTTCACCCAGCACCAC ACTGCCTGCATTGATAACAATGAATGCACTTCTGAGATCAACCTGTGTGGGTCCAAGGGCGTTTGCCAGA ATACACCAGGAAGCTTCACCTGTGAATGCCAGCGGGGATTCTCCCTCGATCAGAGTGGTGCCAGCTGTGA AGATGTGGACGAGTGTGAGGGTAACCACCGCTGCCAACATGGCTGCCAGAACATCATTGGAGGCTATAGG TGCAGCTGCCCCCAGGGCTACCTCCAGCACTACCAGTGGAACCAGTGTGTAGACGAAAACGAGTGCCTGA GTGCACATGTCTGTGGGGGAGCCTCCTGCCACAACACCCTGGGGAGTTACAAGTGCATGTGTCCTGCTGG CTTCCAGTACGAACAGTTCAGTGGAGGCTGCCAAGATATCAACGAGTGTGGCTCATCCCAGGCCCCCTGC AGTTATGGTTGCTCTAATACTGAGGGTGGCTACCTATGTGGCTGTCCTCCAGGATACCTCCGGATAGGCC AAGGGCATTGTGTTTCTGGAATGGGCATGGGCCGAGGTGGCCCAGAGCCACCTGCCAGTGGCGAGATGGA TGACAACTCACTGTCCCCAGAGGCCTGCTATGAGTGTAAGATCAATGGCTACCCAAAACGAGGCAGGAAA CGGAGAAGCACGAATGAAACAGATGCCTCTGACATCCAGGATGGGTCTGAAATGGAAGCCAACGTGAGCC TCGCGAGCTGGGATGTGGAGAAGCCAGCTAGCTTTGCTTTCAATATTTCCCATATCAATAACAAAGTCCG AATCCTAGAGCTCCTGCCGGCCCTTACAACTCTGATGAACCACAACAAATACTTGATCGAGTCTGGAAAT GAATATGGCTTCTTTAAAATCAACCAGAAAGAAGGGGTCAGCTACCTCCACTTCACGAAGAAGAAGCCAG TGGCTGGAACCTACTCCTTACAAATCAGCAGTACTCCACTTTATAAAAAGAAAGAACTTAACCAGTTAGA AGACAGATATGACAAAGACTACCTCAGCGGTGAACTGGGCGATAACCTGAAGATGAAAATTCAGATCTTG CTGCATTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_031825
Insert Size	8619 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031825.1, NP_114013.1
RefSeq Size	8942 bp
RefSeq ORF	8619 bp
Locus ID	83727
Gene Summary	modulates early organogenesis and vascularization of the rat kidney [RGD, Feb 2006]

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