Polr1a (NM_031772) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Rpa1; Rpo1-4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202782 representing NM_031772 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCCTCCAAGCACACGCCATGGCGGCGGCTTCAGGGCATTTCCTTCGGGATGTATTCGGCCGAGG AGCTCAAGAAGTTAAGTGTTAAGTCCATTACAAATCCTCGCTATGTGGACAGCCTGGGAAACCCTTCGGC AGATGGCCTGTATGATTTGGCTTTGGGTCCTGCAGATTCCAAAGAAGTATGCTCTACCTGTGTGCAGGAC TTCAACAACTGCTCCGGACACCTGGGCCACATTGACCTCCCGCTCACAGTGTACAACCCTCTCCTCTTCG ATAAACTCTACCTGCTGCTGCGAGGCTCCTGTTTAAATTGTCATATGCTGACTTGTCCTCGGGCTGCAAT TCACCTATTGGTCTGCCAGCTGAAGGTCCTAGACGTTGGAGCCCTACAAGCAGTCTATGAGCTTGAGCGG ATTCTCAGCAGGTTTCTGGAAGAAACTTCGGATCCTTCTGCCTTCGAAATTCAAGAAGAGTTAGAAGAGT ACACAAGCAAAATTCTGCAGAATAACCTCCTGGGGTCCCAGGGTGCACATGTGAAAAATGTGTGCGAGAG CAGAAGCAAACTTGTCGCTCACTTCTGGAAGACACACATGGCTGCTAAGCGCTGTCCACACTGCAAGACG GGACGGTCAGTGGTTCGAAAGGAGCACAATAGTAAGCTAACAATAACATATCCAGCCATGGTGCACAAGA AGTCTGGCCAGAAGGATGCAGAACTCCCAGAAGGAGCACCAGCAGCTCCAGGAATTGATGAGGCTCAGAT GGGGAAACGAGGATACTTGACCCCAAGCAGCGCCCAAGAACATCTTTTCGCCATTTGGAAGAATGAAGGA TTCTTCCTGAATTATCTTTTTTCGGGATTGGATGATATTGGCCCAGAGTCCAGTTTCAACCCCAGTATGT TCTTTCTAGATTTCATCGTGGTACCGCCATCCAGGTATCGTCCCATCAATCGCCTGGGTGACCAGATGTT CACTAATGGCCAGACAGTGAATTTGCAAGCGGTCATGAAGGATGCAGTTTTGATCCGGAAACTTCTGGCA GTGATGGCCCAAGAACAGAAACTGCCCTGTGAGATGACAGAAATCACCATAGACAAGGAAAATGACTCCT CGGGTGCTATCGACCGGTCCTTTCTGAGTTTGCTTCCAGGACAGTCTCTCACAGATAAACTTTACAATAT TTGGATTCGCCTCCAGAGCCACGTCAATATTGTGTTTGACAGTGACATGGACAAACTGATGCTGGAAAAG TACCCTGGCATAAGACAGATCCTCGAGAAGAAGGAAGGCCTGTTCCGGAAGCACATGATGGGAAAGCGAG TGGACTACGCAGCCCGCTCGGTCATCTGCCCAGATATGTACATCAACACCAATGAGATCGGGATTCCCAT GGTATTTGCCACAAAACTGACTTACCCCCAGCCAGTTACCCCCTGGAACGTGCAGGAACTGAGACAGGCA GTCATCAATGGACCCAATGTGCACCCAGGAGCCTCCATGGTCATCAACGAGGATGGCAGTCGCACAGCCC TGAGCGCAGTGGATGCTACACAGCGGGAAGCTGTGGCCAAACAGCTCCTGACACCGTCCACAGGGATCCC TAAGCCTCAGGGAGCAAAAGTTGTGTGCCGGCATGTGAAGAATGGGGACATTCTCCTCCTGAACCGACAG CCCACCTTGCACAGACCCTCCATCCAGGCCCACCGTGCCCACATTCTGCCTGAGGAAAAAGTCCTGCGCC TTCACTATGCCAACTGCAAGGCCTACAATGCTGACTTCGATGGAGACGAGATGAATGCCCACTTCCCCCA GAGTGAGCTGGGCCGAGCTGAAGCCTATGTCCTGGCCTGCACTGACCAGCAGTATCTTGTTCCCAAGGAT GGCCAGCCCTTGGCAGGACTGATTCAGGATCACATGGTGTCGGGTGCAAACATGACCATTCGGGGATGCT TTTTCACCCGGGAACAGTACATGGAGCTGGTGTACCGAGGGCTCACAGACAAAGTGGGACGCGTGAAGCT CTTTCCTCCTGCCATCCTGAAGCCCTTCCCGCTGTGGACAGGAAAGCAGGTCGTGTCGACCCTCCTCATA AACATAATCCCAGAGGACTACACCCCCCTGAATTTAACTGGAAAGGCAAAAATCGGCAGTAAAGCCTGGG TGAAGGAAAAGCCTCGGCCTGTTCCTGACTTTGACCCTGACTCCATGTGTGAGTCCCAGGTGATCATCAG GGAAGGGGAGCTGCTCTGCGGGGTGCTGGACAAGGCACACTATGGGAGCTCTGCCTACGGCCTGGTCCAC TGCTGCTACGAGATCTATGGGGGTGAGACCAGCGGCAGGGTTCTCACCTGCTTAGCCCGCCTCTTCACTG CCTACCTACAGCTCTATCGAGGTTTCACCTTGGGTGTGGAAGACATTTTGGTTAAGCCAAATGCAGATGT GATGAGACAGCGCATCATTGAAGAATCTACCCAGTGTGGACCCCGGGCTGTCAGGGCTGCGTTGAACCTG CCAGAGGCTGCATCATGTGATGAGATCCAGGGGAAATGGCAGGATGCCATCTGGCGCAAAGACCAGAGGG ATTTTAACATGATTGATATGAAGTTCAAGGAGGAAGTGAACCATTACAGCAATGAGATTAACAAGGCATG TATGCCTTTTGGCTTGCACAGACAATTCCCTGAAAACAACCTGCAGATGATGGTGCAGTCGGGAGCCAAA GGGTCGACCGTGAACACGATGCAAATCTCTTGCCTGCTGGGCCAAATTGAGTTGGAAGGTCGGAGACCCC CACTGATGGCGTCTGGCAAGTCACTGCCCTGCTTTGAGCCTTACGAGTTCACCCCAAGGGCTGGTGGCTT TGTCACTGGAAGGTTCCTTACCGGTATCAGGCCACCTGAGTTCTTCTTCCACTGCATGGCGGGGCGAGAA GGCCTGGTGGATACTGCTGTCAAGACCAGCCGTTCTGGCTATCTCCAAAGGTGTATCATCAAGCACCTGG AGGGTCTGGTCATCCAGTACGATCTCACGGTCCGTGACAGTGATGGCAGTGTGGTACAGTTCCTGTATGG GGAGGATGGCCTGGACATCCCCAAGACACAGTTCCTACAACCCAAGCAGTTCCCCTTTCTGGCCAGCAAT TATGAGGTGATAATGAAATCCAAGCATCTTCATGAAGTTTTGTCTAGAGCAGATCCCCAGAAAGTGCTCC GCCACTTCAGAGCCATTAAAAAATGGCACCACAGGCATTCCAGTGCCCTGCTGAGAAAAGGCGCCTTCCT GAGTTTTTCCCAGAAGATTCAGGCAGCTGTGAAAGCCTTGAACCTAGAGGGCAAGACCCAGAATGGGCGC AGCCCAGAGACCCAGCAGATGTTACAGATGTGGCATGAATTAGATGAGCAGAGCCGACGGAAATACCAGA AGAGGGCTGCCCCGTGTCCCGACCCTAGCCTTTCTGTGTGGCGCCCAGACATCCACTTCGCATCTGTGTC TGAAACATTTGAAAAAAAGATTGATGACTACAGTCAGGAGTGGGCAGCTCAAGCAGAGAAGAGTCACAAC AGATCAGAGCTTTCTCTTGACAGGCTGAGAACCTTACTGCAGCTCAAGTGGCAGCGCTCACTGTGTGACC CAGGCGAGGCTGTTGGCCTCCTGGCCGCCCAGAGCATCGGAGAGCCCTCCACACAGATGACACTGAACAC CTTCCACTTTGCAGGCAGAGGCGAGATGAACGTCACCCTGGGCATTCCAAGATTGAGGGAGATTCTCATG GTGGCCAGTGCCAACATCAAAACACCCATGATGAGCGTGCCTGTGTTCAACACCAAGAAAGCACTGAGGA GAGTGAAGAGTCTGAAGAAGCAGCTCACCAGGGTGTGCCTGGGGGAGGTATTGCAGAAAGTTGACATCCA GGAGTCCTTCTGTATGGGAGAGAAACAGAATAAATTCCGGGTGTATGAGCTCCGGTTTCAGTTCCTGCCA CATGCATATTACCAGCAGGAGAAGTGCCTGAGACCCGAGGACATCTTGCACTTCATGGAAACCAGATTCT TTAAGCTACTAATGGAAGCTATCAAAAAGAAGAATAGCAAAGCATCAGCCTTCAGAAGTGTGAATACCAG GAGAGCTACACAGAAGGACCTCGATGACACTGAGGACTCGGGGAGGAATCGGAGAGAGGAGGAAAGGGAT GAGGAAGAGGAGGGGAACATTGTGGATGCTGAAGCTGAGGAGGGGGATGCAGATGCTTCTGACACCAAAC GCAAGGAGAAGCAAGAAGAAGAGGTTGACTATGAGAGTGAGGAAGAAGGGGAGGAGGAAGAGGAAGAGGA TGTACAAGAAGAGGAGAACATCAAAGGTGAAGGTGCCCACCAGACCCATGAGCCAGATGAGGAAGAAGGT TCAGGCCTTGAGGAGGAATCATCCCAGAACCCTCCCTGCCGCCATTCCAGGCCTCAGGGAGCTGAGGCCA TGGAACGCCGAATCCAGGCTGTACGGGAGTCCCATTCGTTCATTGAGGACTACCAGTATGACACAGAGGA GAGCCTGTGGTGCCAGGTGACAGTGAAGCTCCCATTGATGAAGATTAATTTTGACATGAGCTCCTTGGTT GTATCCTTGGCCCACAATGCCATTGTCTATACGACTAAGGGCATCACTCGGTGTCTCCTGAACGAAACGA TCAACAGTAAGAATGAGAAGGAGTTTGTCCTGAACACGGAGGGAATCAACCTCCCCGAGCTGTTCAAGTA CTCCGAGGTGCTGGACCTGCGCCGCCTGTACTCCAACGACATCCATGCTGTGGCCAATACGTACGGCATC GAGGCTGCGCTGCGGGTGATAGAGAAGGAGATTAAGGATGTGTTTGCTGTGTACGGCATTGCGGTTGATC CTCGCCATCTCTCCCTGGTTGCTGATTATATGTGTTTTGAGGGTGTTTATAAGCCACTCAATCGCTTTGG GATCCAGTCAAGCTCCTCCCCTTTGCAGCAGATGACGTTTGAAACCAGCTTCCAGTTTCTGAAACAGGCC ACCATGATGGGATCCCATGATGAACTGAAGTCACCTTCTGCCTGCCTTGTGGTGGGAAAGGTCGTCAAAG GCGGGACAGGCCTGTTTGAGCTCAAGCAGCCCCTAAGATAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_031772
Insert Size	5151 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031772.1, NP_113960.1
RefSeq Size	6018 bp
RefSeq ORF	5151 bp
Locus ID	83581
UniProt ID	O54889
Gene Summary	subunit of RNA polymerase I [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.