Rasgrf2 (NM_053721) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202861 representing NM_053721 Red=Cloning site Blue=ORF Orange=Stop codon CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGCAGAAGAGCGTGCGCTACAACGAGGGGCACGCCCTGTACCTGGCCTTCCTGGCGCGCAAGGAGGGCA CCAAGCGCGGTTTCCTGAGCAAAAAGGCTGCCGAGGCGAGCCGCTGGCACGAGAAGTGGTTCGCTCTCTA TCAGAATGTGCTCTTTTACTTCGAAGGCGAACAGAGCGGCCGCCCAGCGGGCATGTACCTCCTAGAGGGC TGCAGCTGTGAGCGGACGCCCGCCCCACCAAGGACCAACGCAGGACCCGCAGGCGCCCGGGACGCGCTGG ACAAACAGTATTACTTCACTGTCCTTTTTGGTCACGAAGGTCAGAAACCACTGGAGCTGCGCTGTGAGGA GGAGCAAGCAGGGAAAGAGTGGATGGAGGCCATTCACCAAGCCAGTTACGCAGACATTTTGATTGAGAGG GAAGTGTTAATGCAAAAGTACATTCATCTAGTTCAGATTGTGGAGACAGAAAAAATTGCTGCTAACCAAC TCCGACATCAACTCGAAGATCAAGATACCGAAATTGAAAGACTTAAATCAGAGATTGTTGCTCTTAATAA GACCAAGGAACGGATGCGCCCGTATCATACCCATCAAGAGGAGGAGGATCCAGACATCAAGAAGATCAAG AAGGTTCAGAGCTTCATGAGAGGGTGGCTGTGCAGGAGGAAGTGGAAGACCATCGTTCAGGACTACATTT GTTCCCCCCATGCTGAGAGCATGAGGAAGAGGAACCAGATTGTCTTCACGATGGTGGAGGCCGAGTCAGA ATATGTGCACCAGCTCTACATCCTGGTGAACGGTTTCCTCAGGCCTCTGCGCATGGCAGCCAGCTCCAAG AAGCCCCCCATTAGCCATGATGATGTCAGCAGCATTTTCCTCAACAGTGAAACAATCATGTTTCTTCATG AAATATTCCATCAAGGTCTAAAGGCAAGGCTGGCAAATTGGCCCACTTTGGTTTTAGCTGATCTCTTTGA TATTTTGCTGCCTATGCTCAACATTTATCAAGAATTTGTGCGTAATCATCAGTACAGCCTCCAAGTACTT GCCAACTGTAAGCAAAATAGAGATTTTGACAAGCTCTTAAAGCAGTATGAAGGCAACCCTGCCTGTGAGG GAAGGATGCTGGAGACATTCTTGACTTACCCAATGTTTCAGATCCCCAGGTATATCATCACGCTGCACGA ACTCCTGGCTCACACCCCCCATGAACACGTGGAGAGGAAAAGTCTGGAGTTCGCTAAGTCAAAGCTGGAG GAACTGTCCAGAGTGATGCACGACGAAGTCAGTGACACCGAAAACATACGGAAAAATCTTGCCATTGAGA GGATGATCGTGGAGGGCTGCGACATTTTGCTAGACACCAGTCAAACTTTCATCCGACAAGGTTCTCTTAT CCAAGTACCTTCTGTGGAGAGGGGGAAACTCAGTAAAGTCCGCCTGGGCTCCCTGTCTTTGAAAAAGGAA GGCGAGAGACAATGTTTCTTATTCACGAAGCACTTTCTGATCTGTACCCGAAGTTCAGGAGGAAAGCTTC ATTTGCTCAAGACAGGTGGGGTTCTCTCCCTCATTCAGTGTACGTTGATTGAGGAGCCGGACACAAGTGA TGATGACACCAAAGGTCCTGGTCACATGTTCGGACATCTGGATTTTAAAATCGTCGTGGAGCCTCCTGAT GCTGCCCCGTTCACTGTGGTCTTGCTAGCACCTTCGCGCCAGGAGAAGGCTGCCTGGATGAGCGACATCA GCCAGTGCGTGGACAATATCCGATGCAATGGATTGATGACTATAGTCTTCGAAGAGAATTCTAAAGTTAC TGTGCCCCATATGATAAAGTCTGATGCCCGTCTTCATAAAGACGACACTGACATCTGTTTCAGTAAAACA CTCAATTCCTGCAAAGTGCCCCAGATCCGCTACGCCAGCGTGGAGCGCCTCTTGGAGCGGTTGACGGATT TGCGGTTTCTGAGCATCGATTTCCTCAACACCTTCCTGCACACCTACCGCATTTTCACCACGGCTGCCGT AGTGCTGGGGAAGCTCTCCGACATCTACAAGAGGCCTTTCACCTCCATTCCTGTCAGATCACTAGAGTTA TTTTTTGCCACGAGCCAGAACAACAGAGAACATTTGGTCGATGGAAAATCCCCACGTCTTTGTCGCAAGT TCTCTTCCCCACCTCCCCTGGCTGTATCCAGAACGTCCTCCCCAGTGAGGGCCAGAAAGCTGTCTTTGAC ATCTTCCTTGAACTCAAGGATCGGAGCCTTGGACCTGACTACATCCTCATCTTCCAGTAGTCCCACCACC ACTGTCCACAGTCCTGCTGCGTCCCCACCACCTCACACTGCTGTCCCAGAGTCTGCACCAGCCGACCGAG CAGGAGACTCCACAGACATGTCACCTTGTAGATCTCCCTCAACCACTCCCCGGCACCTCCGATATCGCCA GCCTGGAGGACAGGTAGCTGACAGCACCCACTGTGCTGTTTCACCTGCTTCTGCTTTTGCGATCGCCACA GCAGCAGCAGGACATGGGAGTCCGCCAGGATTTAACAACGAGAGGACATGTGACAAGGAGTTCATCATAC GGAGAACAGCCACCAATCGAGTCCTGAATGTCCTCCGCCACTGGGTTTCAAAGCACTCACAGGATTTTGA ACTCAATAATGAACTCAAAATGAATGTTCTGAACTTGCTAGAAGAAGTTCTACGAGACCCGGACCTTCTT CCTCAAGAAAGGAAAGCCACGGCGAACATCCTGAGGGCTCTTTCACAAGATGATCAAGATGACATCCACC TAAAATTAGAGGATATAATTCAAATGACAGACTGTCCAAAGGCCGAGTGCTTTGAGACATTGTCAGCCAT GGAGCTGGCTGAGCAGATCACTCTCCTGGACCACATTGTTTTCAGAAGCATCCCCTATGAAGAGTTTCTT GGGCAGGGCTGGATGAAGCTGGATAAAAACGAAAGGACTCCTTATATCATGAAAACCAGCCAGCACTTCA ATGAGATGAGTAACCTGGTGGCCTCCCAGATAATGAATTATGCTGACATCAGTTCCCGTGCCAATGCCAT TGAGAAGTGGGTAGCAGTGGCGGACATCTGCCGATGTCTACACAACTACAATGGTGTGCTGGAGATCACC TCGGCCTTAAACAGAAGTGCCATCTACAGGCTGAAGAAAACCTGGACCAAGGTGTCCAAGCAGACAAAAG CTCTGATGGACAAACTTCAGAAGACTGTCTCGTCTGAAGGAAGATTTAAAAACCTCAGAGAAACTCTCAA AAACTGTAACCCCCCAGCTGTCCCTTACCTTGGGATGTACTTGACAGACCTGGCATTCATTGAAGAAGGG ACACCGAACTTTACCGAAGAAGGCCTTGTCAATTTCTCCAAAATGCGAATGATATCACACATCATTCGTG AGATACGCCAGTTCCAGCAGACCGCTTACCGCATAGACCAGCAGCCAAAGGTCATACAATACCTGCTTGA CAAAGCCCTTGTCATAGATGAAGATACACTCTATGAGCTGTCACTCAAAATTGAACCTCGACTCCCTGCT TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-MluI
ACCN	NM_053721
Insert Size	3573 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053721.1, NP_446173.1
RefSeq Size	3573 bp
RefSeq ORF	3573 bp
Locus ID	114513
UniProt ID	Q99JE4
Gene Summary	human homolog is involved in stimulating the conversion of the GDP-bound form of Ras into the active form [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.