Cacna1s (NM_053873) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cchl1a3; RGD1565743
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202973 representing NM_053873 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGTCCTCACCCCAGGATGAGGGCCTGAGGAAGAAGCAGCCTAAGAAACCCGTCCCTGAGATTC TGCCGAGGCCACCCCGGGCCTTGTTCTGCTTGACTCTCCAGAACCCCCTGAGGAAGGCCTGCATCAGCGT CGTGGAGTGGAAACCCTTCGAGACCATCATCTTGCTCACCATCTTCGCCAACTGTGTGGCCCTGGCAGTG TACCTGCCCATGCCAGAGGATGACAACAACACTCTGAACCTTGGCCTGGAGAAACTGGAGTACTTCTTCC TCATCGTCTTCTCCATTGAGGCTGCTATGAAGATCATAGCCTATGGCTTCCTATTCCATCAGGATGCTTA CCTGCGCAGTGGCTGGAACGTGCTGGACTTCATCATTGTCTTTCTGGGTGTCTTCACCGCGATTCTGGAA CAAGTTAACATCATTCAAACCAACACGGCCCCCATGAGCAGCAAAGGAGCCGGCTTGGATGTGAAGGCCC TGCGAGCTTTCCGCGTGCTCAGACCCCTGCGGCTGGTGTCAGGGGTGCCCAGCCTTCAGGTAGTCCTCAA CTCCATCTTCAAGGCCATGCTCCCCCTGTTCCACATCGCCCTGCTCGTCCTCTTCATGGTCATCATCTAT GCCATCATCGGGCTGGAGCTCTTCAAGGGCAAGATGCACAAGACGTGCTACTTCATTGGGACAGACATCG TGGCCACAGTAGAGAATGAGAAACCATCACCCTGTGCTAGAACTGGCTCCGGGCGCCCCTGCACCATCAA CGGCAGCGAGTGCCGAGGAGGTTGGCCAGGACCCAACCATGGCATCACCCACTTCGACAACTTTGGCTTC TCTATGCTCACTGTGTACCAGTGCATCTCCATGGAGGGGTGGACCGATGTCCTGTACTGGGTCAATGATG CCATCGGGAACGAGTGGCCCTGGATCTACTTTGTCACCCTCATTCTGCTGGGCTCCTTCTTCATCCTGAA CCTGGTGCTGGGTGTCCTGAGTGGAGAATTCACCAAGGAGCGAGAGAAGGCCAAGTCCAGAGGAACCTTC CAAAAGCTCCGGGAGAAGCAGCAGCTGGAGGAAGACCTTCGTGGTTACATGAGTTGGATCACACAGGGCG AGGTCATGGACGTGGACGACTTGAGAGAAGGCAAGCTGTCTTTGGATGAAGGGGGCTCGGATACAGAGAG CTTATATGAAATCGAGGGCTTGAACAAAATCATCCAATTCATTCGGCACTGGCGGCAGTGGAATCGCGTC TTCCGCTGGAAGTGCCATGACCTAGTGAAATCCAAGGTCTTCTATTGGCTGGTGATCCTGATTGTGGCCC TCAACACCCTGTCCATTGCCTCAGAGCACCACAACCAGCCACTCTGGCTGACGCATTTGCAAGATGTCGC CAACCGCGTGCTGCTGGCCCTCTTCACCATCGAGATGCTCATGAAGATGTACGGGCTGGGCCTGCGCCAG TACTTCATGTCCATCTTCAACCGCTTCGACTGCTTCGTGGTGTGCAGCGGCATCCTGGAGATCTTGCTGG TGGAGTCGGGAGCCATGACGCCCCTGGGCATCTCTGTGCTCCGCTGCATCCGCCTCCTGAGGCTCTTCAA GATCACCAAGTACTGGACATCGCTGAGCAACCTGGTGGCCTCGCTGCTCAACTCCATCCGCTCTATCGCT TCCCTCTTGCTGCTGCTCTTTCTTTTCATGATCATCTTTGCCCTGCTAGGCATGCAGCTCTTTGGAGGCA GATACGACTTTGAAGACACGGAAGTACGACGAAGCAACTTTGACAACTTCCCCCAGGCCCTCATCAGTGT CTTCCAGGTCCTGACGGGTGAAGACTGGAACTCTGTGATGTACAATGGGATCATGGCCTACGGAGGCCCG TCCTACCCTGGCGTGCTCGTGTGCATTTATTTCATCATCCTTTTTGTCTGTGGTAACTACATCCTTCTCA ATGTCTTCCTGGCTATCGCTGTGGACAACCTGGCAGAGGCTGAGAGCCTGACATCTGCCCAGAAGGCCAA GGCTGAGGAGAGAAAGCGCAGGAAGATGTCTAGAGGGCTCCCAGACAAGTCAGAAGAGGAGAGATCCACA ATGACCAAGAAGCTGGAGCAGAAACCCAAGGGTGAAGGCATCCCCACCACTGCCAAGCTGAAAATCGATG AGTTTGAATCGAACGTCAATGAGGTGAAAGACCCCTACCCTTCAGCTGACTTCCCAGGGGACGACGAGGA AGATGAGCCTGAGATCCCAGCAAGCCCCCGACCACGCCCTCTGGCTGAGCTGCAGCTGAAAGAGAAAGCG GTGCCCATCCCAGAGGCCAGCTCCTTCTTCATCTTCAGCCCCACCAATAAGATCCGCGTCCTATGTCACC GCATTGTCAACGCCACCTGGTTCACCAACTTTATCCTACTCTTCATCCTGCTCAGTAGTGCTGCTCTGGC CGCTGAGGACCCCATCCGGGCCGACTCCATGAGGAATCAGATCCTTGAGTATTTTGATTATGTGTTCACT GCTGTTTTCACTGTGGAGATCGTCCTTAAGATGACCACCTATGGAGCCTTCCTGCACAAAGGCTCCTTCT GCCGCAACTACTTCAACATCCTGGACCTGCTGGTAGTGGCTGTGTCTCTCATCTCCATGGGCCTCGAGTC CAGCGCCATCTCCGTGGTGAAGATCTTGAGGGTCCTGAGGGTGTTGCGACCACTCCGGGCCATAAACAGA GCCAAAGGGTTGAAGCACGTGGTGCAGTGCGTGTTTGTTGCTATCCGCACCATCGGGAACATCGTGCTGG TCACCACGCTCCTGCAGTTCATGTTTGCATGCATCGGAGTCCAGCTTTTCAAGGGAAAATTCTACAGCTG CAATGATTTATCCAAGATGACTGAAGAGGAGTGCAGGGGCTACTACTACATATACAAGGATGGAGACCCC ACGCAGATAGAGTTGCGACCCCGTCAGTGGATACACAATGACTTCCACTTTGACAATGTCCTGTCTGCTA TGATGTCACTCTTCACGGTCTCCACCTTTGAGGGTTGGCCTCAGCTACTGTACAAGGCCATAGACTCCAA TGAGGAGGACACAGGCCCCGTCTACAACAACCGAGTGGAGATGGCCATCTTCTTCATCATCTACATCATT CTCATCGCCTTCTTCATGATGAACATCTTTGTGGGCTTCGTCATCGTCACCTTCCAGGAGCAGGGGGAGA CTGAGTATAAGAACTGTGAGCTGGACAAGAACCAGCGCCAATGTGTGCAGTATGCCCTGAAGGCCCGCCC GCTGAGGTGTTACATCCCCAAAAACCCATACCAGTATCAGGTGTGGTATGTCGTCACTTCCTCCTACTTT GAATACCTGATGTTTGCTCTCATCATGCTCAACACTATCTGCCTAGGCATGCAGCATTACAACCAGTCGG AACAGATGAACCACATATCGGACATCCTCAATGTGGCTTTCACCATCATCTTCACCCTGGAGATGATCCT CAAGCTCATAGCTTTCAAGCCCAGGGGCTATTTTGGAGACCCCTGGAACGTGTTTGACTTTCTAATCGTC ATTGGCAGCATCATTGACGTTATCCTGAGTGAGATCGATACTTTGCTGGCCTCCAGCGGGGGACTATATT GCCTGGGTGGAGGCTGCGGGAACGTTGACCCAGATGAGAGCGCCCGCATCTCCAGTGCCTTCTTCCGCCT GTTCCGAGTCATGCGACTGATCAAGCTGCTGAGCCGGGCGGAGGGTGTGCGCACCCTGCTCTGGACGTTC ATCAAGTCCTTCCAGGCCCTGCCGTATGTGGCTTTGCTGATCGTCATGCTCTTCTTCATCTACGCTGTCA TCGGCATGCAGATGTTCGGGAAGATCGCCATGGTGGACGGGACGCAAATAAACCGGAACAACAACTTCCA GACTTTCCCACAAGCTGTGCTGCTGCTCTTCAGGTGTGCCACAGGGGAAGCCTGGCAGGAGATCCTGCTG GCCTGCAGCTACGGGAAACGCTGCGACCCGGAGTCTGACTACGCACCGGGGGAGGAGTACGCGTGTGGCA CCAACTTTGCCTACTACTACTTCATCAGCTTCTACATGCTCTGCGCCTTCCTGATCATCAACCTCTTTGT GGCTGTCATCATGGACAACTTTGACTACCTCACAAGGGACTGGTCCATTCTGGGCCCTCACCACCTGGAT GAGTTCAAGGCCATCTGGGCTGAGTACGACCCAGAGGCTAAGGGCAGGATCAAGCACCTGGACGTGGTGA CCCTGCTGAGGAGGATCCAGCCCCCTCTGGGCTTTGGGAAGTTCTGCCCACACAGGGTAGCATGTAAGAG GCTGGTGGGCATGAACATGCCCCTGAATAGTGATGGCACAGTCACCTTCAATGCCACACTCTTCGCCCTG GTACGCACAGCCCTCAAGATCAAGACAGAAGGCAATTTTGAACAAGCCAATGAGGAGCTGAGAGCCATCA TCAAAAAGATCTGGAAGAGAACCAGCATGAAGCTCCTGGACCAAGTCATCCCTCCAATAGGAGATGACGA GGTGACAGTGGGGAAGTTCTATGCCACATTCCTCATCCAGGAGCACTTTCGGAAGTTCATGAAGCGCCAG GAGGAATATTATGGGTATCGACCCAAAAAGGACACAGTTCAGATACAGGCTGGATTGCGGACCATAGAGG AAGAGGCAGCTCCTGAGATCCACCGAGCCATCTCTGGAGACCTGACGGCGGAGGAGGAGCTGGAGAGGGC CATGGTAGAGGCTGCCATGGAGGAGGGGATATTCCGGAGAACTGGAGGCCTGTTTGGTCAAGTGGACAAC TTCCTGGAAAGGACCAACTCTCTGCCCCCTGTCATGGCTAACCAAAGACCCCTCCAATTTGCCGAAATGG AGATGGAAGAGCTGGAGTCACCAGTCTTTTTGGAGGACTTCCCTCAGAACCCAGGCACCCACCCTCTTGC TCGTGCCAATACCAACAACGCCAATGCCAATGTTGCCTATGGCAACAGCAGCCATAGAAACAGCCCAGTG TTTTCCAGCATCCGCTATGAAAGGGAGCTCCTGGAAGAGGCAGGCAGGCCTGTTACCAGAGAAGGACCCT TCAGTCAGCCCTGCAGCGTCTCTGGAGTTAACAGCAGATCCCATGTGGACAAACTGGAAAGACAGATGTC CCAAAGAAGAATGCCCAAAGGCCAGGTGCCTCCTTCCCCATGCCAGCTCTCCCAGGAAAAGCATCCTGTG CACGAAGAGGGGAAGGGCCCCAGGTCATGGTCTACGGAAACATCTGACAGTGAGAGCTTTGAGGAGCGTG TTCCCAGGAATTCGGCTCACAAGTGCACAGCCCCAGCTACCACCATGCTGATCCAGGAGGCTCTGGTTCG AGGAGGACTAGACTCCTTAGCAGCAGATGCTAACTTCGTCATGGCAACAGGCCAGGCCCTGGCAGATGCC TGCCAGATGGAACCAGAGGAAGTCGAGGTGGCAGCAACAGAGCTACTGAAGCGAGAATCCCCAAAGGGCG GGGCCATGCCCAGGGAGCCTTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_053873
Insert Size	5553 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053873.1, NP_446325.1
RefSeq Size	6125 bp
RefSeq ORF	5553 bp
Locus ID	682930
UniProt ID	Q02485
Gene Summary	skeletal isoform and subunit of voltage dependent L-type Ca+2 channel; may mediate renal Ca+2 reabsorption; may play a role in bone metabolism [RGD, Feb 2006]

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