Cacna1i (NM_020084) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203004 representing NM_020084 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACAGCAACTTACCGCCCTCATCTGCAGCAGCCCCGGCCCCTGAGCCGGGAATCACTGAGCAGC CGGGGCCCCGGAGTCCCCCTCCATCCCCTCCAGGCCTGGAGGAGCCATTGGAAGGAACCAACCCTGACGT CCCACATCCAGACCTGGCTCCTGTTGCTTTCTTCTGCCTGCGCCAGACCACGAGCCCACGGAACTGGTGC ATCAAGATGGTTTGTAACCCGTGGTTCGAGTGTGTGAGCATGCTGGTTATTCTGCTGAACTGTGTGACCC TGGGCATGTACCAGCCATGTGATGACATGGAGTGCCTGTCGGACCGTTGCAAGATCCTGCAGGTCTTCGA TGACTTCATCTTCATCTTCTTTGCCATGGAGATGGTGCTTAAGATGGTGGCCCTGGGCATTTTTGGCAAG AAGTGCTACCTCGGAGACACATGGAACCGCCTGGATTTCTTCATTGTCATGGCAGGGATGGTTGAGTACT CTCTGGACCTACAGAACATCAACCTGTCAGCCATCCGCACTGTGCGTGTCCTGAGGCCTCTCAAAGCCAT CAACCGTGTACCCAGCATGCGGATCCTGGTGAACCTGCTGCTCGACACGCTGCCCATGCTGGGGAACGTG CTCCTGCTCTGTTTCTTCGTCTTCTTCATCTTCGGCATCATTGGCGTGCAGCTCTGGGCAGGCCTGCTAC GGAACCGCTGCTTCCTGGAAGAGAACTTCACCATACAAGGGGATGTGGCCCTGCCCCCTTATTACCAACC AGAGGAGGATGACGAGATGCCCTTTATCTGCTCCCTGACTGGGGACAATGGCATCATGGGCTGCCACGAG ATCCCCCCACTGAAGGAGCAGGGCCGGGAATGCTGCCTGTCCAAAGATGATGTGTATGACTTCGGGGCGG GGCGCCAGGACCTCAACGCCAGCGGTCTGTGCGTCAACTGGAACCGCTACTACAACGTCTGCCGCACGGG CAACGCCAACCCTCACAAGGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATTGTGATTTTCCAG GTGATCACTCTGGAAGGCTGGGTGGAGATCATGTACTATGTGATGGACGCACATTCTTTCTACAACTTCA TCTACTTCATTCTGCTCATCATAGTGGGCTCCTTCTTCATGATCAACTTGTGCCTCGTTGTCATAGCAAC CCAGTTCTCTGAGACCAAGCAACGGGAGCACCGGCTGATGCTGGAGCAACGCCAGCGCTACCTGTCCTCC AGCACGGTGGCCAGTTACGCTGAGCCCGGTGATTGCTATGAGGAGATCTTCCAATATGTCTGTCACATCC TTCGCAAAGCCAAGCGCCGTGCCCTAGGCCTCTACCAGGCCCTGCAGAACCGGCGCCAGGCCATGGGCCC GGGGACACCAGCCCCTGCCAAGCCTGGGCCCCATGCCAAGGAGCCCAGCCACTGCAAGCTGTGCCCACGA CACAGCCCCCTGGACCCCACTCCCCACACACTGGTGCAGCCCATCTCTGCCATTCTGGCCTCTGACCCCA GCAGCTGCCCTCACTGCCAGCACGAGGCAGGCAGGCGGCCCTCTGGCCTGGGCAGCACTGACTCAGGCCA GGAAGGCTCAGGTTCTGGTGGCTCTGCAGAGGCCGAAGCCAATGGGGATGGACCCCAGAGCAGTGAGGAT GGGGTCTCCTCGGACCTGGGGAAGGAGGAGGAACAGGAGGACGGGGCAGCCCGACTGTGTGGGGATGTGT GGCGCGAGACACGAGAAAAGCTGCGGGGCATCGTGGACAGCAAGTACTTCAACAGAGGTATCATGATGGC TATCCTGGTGAACACAGTCAGCATGGGCATCGAGCACCACGAACAGCCCGAGGAGCTGACCAACATCCTG GAGATCTGCAATGTGGTCTTCACCAGTATGTTTGCCCTGGAGATGATCCTGAAACTGGCCGCCTTTGGGC TCTTCGACTACCTGCGGAACCCTTACAACATCTTTGACAGCATCATCGTCATCATCAGCATCTGGGAAAT CGTGGGGCAGGCAGACGGTGGCCTGTCTGTGCTGCGCACCTTCCGGTTGCTGCGGGTGCTGAAGCTGGTG CGCTTCATGCCGGCGCTGCGGCGCCAGCTCGTGGTGCTCATGAAGACCATGGACAACGTGGCCACCTTCT GCATGCTACTCATGCTGTTCATCTTCATCTTCAGCATCCTTGGGATGCATATCTTTGGCTGCAAATTCAG CCTCCGCACGGACACGGGAGACACCGTTCCTGACAGGAAGAACTTCGATTCCTTACTGTGGGCCATCGTC ACAGTGTTCCAGATCCTCACTCAGGAGGACTGGAACGTTGTCCTGTACAATGGCATGGCCTCCACCACCC CCTGGGCCTCCCTCTATTTTGTTGCCCTCATGACCTTTGGCAACTACGTTCTCTTCAATCTCCTGGTGGC TATCCTGGTAGAGGGTTTCCAGGCTGAGGGTGATGCTAATCGTTCCTACTCTGATGAGGACCAGAGCTCA TCCAATTTGGAGGAGTTTGACAAGCTCCCAGAGGGCCTGGACAACAGTAGAGATCTCAAGCTCTGCCCAA TACCCATGACACCCAATGGACACCTGGACCCTAGCCTCCCTCTGGGTGCGCATCTGGGTCCTGCTGGTAC CATGGGTACTGCCCCCCGCCTCTCACTGCAGCCAGACCCGGTACTGGTGGCCCTAGACTCTCGGAAAAGC AGTGTCATGTCCCTGGGCAGGATGAGCTATGATCAGCGATCCTTGTCCAGCTCCCGGAGCTCCTACTACG GGCCCTGGGGCCGCAGTGGGACCTGGGCTAGCCGCCGCTCCAGCTGGAACAGCCTGAAACACAAGCCGCC CTCAGCTGAGCATGAGTCCTTACTGTCTGGGGAGGGTGGAGGTAGCTGCGTCAGGGCCTGTGAAGGCGCC CGGGAGGAGGCGCCAACTCGCACCGCACCCCTGCATGCTCCACACGCGCACCACGCGCACCATGGACCCC ACCTGGCACACCGTCACCGACACCACCGCCGGACTCTGTCCCTTGATACCAGGGACTCTGTTGACCTGGG AGAGCTGGTGCCCGTGGTGGGTGCCCACTCACGGGCCGCTTGGAGGGGGGCGGGTCAGGCCCCTGGGCAC GAGGACTGCAATGGCAGAATGCCCAACATAGCCAAGGATGTCTTCACCAAGATGGATGACTGCCGCGACC GCGGGGAGGACGAGGAGGAGATCGACTATACCCTGTGTTTCCGGGTCCGCAAGATGATTGATGTGTACAA GCCGGACTGGTGCGAAGTCCGCGAGGACTGGTCGGTCTACCTCTTCTCCCCCGAGAACAAGTTCCGGATC CTGTGTCAGACTATCATTGCTCACAAGCTTTTTGACTACGTGGTCTTGGCCTTTATCTTCCTCAACTGTA TCACCATTGCTCTGGAGAGACCCCAGATTGAAGCTGGTAGCACTGAGCGCATCTTCCTCACGGTGTCTAA CTACATCTTCACAGCCATCTTCGTGGGCGAGATGACACTGAAGGTGGTTTCTCTGGGCCTGTACTTTGGT GAGCAGGCGTACCTGCGTAGCAGCTGGAATGTACTGGATGGTTTCCTGGTCTTTGTGTCCATCATCGATA TCGTAGTGTCCGTGGCCTCTGCTGGGGGAGCCAAGATTCTGGGGGTCCTCCGGGTCCTGCGGCTCCTGCG TACCTTACGTCCTTTGAGGGTTATCAGCCGGGCCCCTGGGCTGAAGCTGGTGGTAGAGACGCTCATCTCC TCCCTCAAGCCCATTGGGAACATCGTCCTCATCTGCTGTGCCTTCTTCATCATCTTCGGCATCCTGGGGG TGCAGCTTTTCAAAGGCAAGTTCTACCATTGTTTGGGAGTGGACACCCGAAACATCACCAACCGATCTGA CTGCGTGGCGGCCAACTACCGCTGGGTGCATCACAAATACAACTTTGACAACCTGGGCCAGGCATTGATG TCCCTCTTTGTCTTGGCCTCCAAGGACGGCTGGGTGAACATCATGTATAATGGATTAGATGCTGTTGCTG TGGACCAGCAGCCAGTGACGAACCACAACCCCTGGATGCTACTGTACTTCATTTCGTTCCTGCTCATCGT CAGCTTCTTTGTGCTCAACATGTTTGTGGGCGTGGTCGTGGAGAACTTCCACAAGTGCCGGCAGCACCAG GAGGCTGAGGAGGCGCGGAGGCGTGAGGAGAAACGGCTGCGGCGCCTGGAAAAGAAGCGCCGTAAGGCTC AGAGGCTGCCCTACTATGCTACCTACTGTCCCACAAGGCTGCTCATCCACTCCATGTGCACCAGCCACTA CCTGGACATCTTCATTACCTTCATCATCTGCCTCAATGTTGTCACCATGTCCCTGGAGCACTACAACCAG CCTACATCCCTAGAGACAGCCCTTAAGTACTGCAACTACATGTTCACCACTGTCTTTGTGCTGGAGGCTG TGCTGAAGCTGGTGGCATTTGGCCTGAGGCGTTTCTTCAAGGACCGATGGAACCAGCTGGACCTGGCCAT TGTGCTGCTGTCCGTCATGGGCATCACACTGGAGGAGATCGAGATCAATGCCGCCCTTCCCATCAACCCC ACCATCATCCGTATCATGCGTGTTCTGCGTATCGCCCGGGTGTTGAAGCTATTGAAGATGGCCACAGGAA TGCGGGCCCTGCTGGACACAGTGGTACAGGCTCTGCCCCAGGTGGGCAACCTGGGCCTGCTCTTCATGCT GCTCTTCTTCATCTATGCTGCTCTGGGAGTGGAGCTCTTCGGAAAGCTGGTCTGCAATGACGAGAACCCG TGTGAGGGCATGAGCCGGCACGCCACCTTTGAAAACTTCGGCATGGCCTTCCTCACGCTCTTCCAGGTCT CCACAGGCGATAACTGGAATGGAATTATGAAGGACACCCTGCGAGACTGTACCCATGATGAGCGCAGCTG CCTAAGCAGCCTGCAGTTTGTGTCACCGCTCTACTTTGTGAGCTTCGTGCTCACAGCTCAGTTCGTGCTC ATCAACGTGGTGGTGGCCGTGCTGATGAAACATCTGGATGACAGCAACAAGGAGGCCCAGGAGGATGCAG AGATGGATGCTGAGATCGAGCTGGAGATGGCCCATGGCCTCGGCCCCTGCCCTGGCCCCTGCCCTGGTCC CTGCCCCTGCCCCTGGCCCCTAGCCCCTGTCCTGGCCCGAGGCTGCCCGCTAGTTCTCCTAGGGGCTACC GGGGCGAGGATCGGGAGGGGCAGGTGCTGGAGGCGACACCGAGAGTCACCTGTGCCGAGCACTCGCTATT CTCCAGCCCAGGAGACCCTGTGGCTGGACAGCGTCTCTTTAATCATCAAGGACTCCTTGGAGGGGGAGCT GACCATCATTGACAACCTGTCTGGGTCCGTCTTCCACCACTACGCCTCACCTGACGGCTGTGGCAAGTGT CACCATGACAAGCAAGAGGTGCAGCTGGCTGAGACAGAGGCCTTCTCCCTGAACTCAGACAGGTCTTCAT CCATCCTGCTGGGGGATGACCTGAGTCTTGAGGACCCCACGGCCTGCCCACCTGGCCGCAAAGACAGCAA GGGTGAGCTGGACCCACCTGAGCCCATGCGTGTGGGAGACCTGGGCGAATGCTTCTTCCCCTTGTCCTCT ACGGCCGTCTCGCCGGATCCAGAGAACTTCCTGTGTGAGATGGAGGAGATCCCATTCAACCCTGTCCAGT CCTGGCTCAAACATGACAGCAGTCAAGCACCCCCGAGCCCTTTCTCCCCGGATGGCTCCAGCCCTCTCCT GCAGATGCCTGCTGAGTTCTTCCACCCTGCTGTGTCTGCCAGCCAGAAAGGCCCAGAAAAGGGCACTGGC ACTGGAACCCTGCCCAAGATTGCACTTCAGGGGTCCTGGGCATCGCTGAGGTCACCGAGTGTCAACTGCA CCCTCCTCCGGCAGGCCACCGGGAGCGACACGTCGCTGGACGCCAGCCCCAGCAGCTCAGCGGGCAGCCT ACAGACCACACTGGAAGACAGTCTGACTCTGAGTGACAGTCCCCGGCGTGCCCTGGGGCCGCCGGTCCAG GTGCCTGGGCCACGGGCTAGCCTGTCACCGGCCACCCGGCGCCGCCTCAGCCTGCGGGGCCGTGGCCTGT TTAGTCTGCGTGGGCTGCGGGCCCATCAGCGTAGCCACAGCAGTGGCGGCTCCACCAGCCCTGGCTGCAC TCACCACGACTCCATGGACCCCTCTGATGAGGAGGGCCGCGGGGGCGCAGGTGGCGGGGGTGCAGGCAGT GAGCACTCGGAGACCCTCAGCAGCCTGTCACTCACATCTCTCTTCTGCCTACCACCCACGCTGCCACCAC CCGGCCTCACCCCAGCCAGGAAGTTCAACAGTACCAGCAGCCTGGCCGCTGGACCTGGCCGTCCTGGCTC CACTGTCTCAGCCCGTGGTCTGGTCAGAAGCCCCTCATGGGCTGCAGACCGCAGCAAGGACCCACCAGGC CAAGCTCAGCTGGTCTCAGGCTTGGGCTCCTCAGCACCCGGGCCACAGCCACCTCCCGGGGAGTCAACTG ATGCTGCGAGCAAAAGGAAGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_020084
Insert Size	6606 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_020084.3, NP_064469.3
RefSeq Size	6709 bp
RefSeq ORF	6606 bp
Locus ID	56827
UniProt ID	Q9Z0Y8
Gene Summary	low voltage-activated calcium channel subunit; important for calcium ion transport into nerve, muscle and endocrine cells [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.