Grin2d (NM_022797) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	GluN2D
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203008 representing NM_022797 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGGCGCCGGTGGCCCCCGCGGCCCTCGGGGCCCCGCTAAGATGCTGCTGCTGCTGGCGCTGGCGT GCGCCAGCCCGTTCCCGGAGGAGGTGCCGGGGCCGGGCGCGGTCGGCGGGGGCACGGGCGGGGCGCGGCC ACTCAACGTGGCGCTTGTCTTCTCCGGCCCGGCATATGCGGCAGAGGCGGCGCGCTTGGGCCCGGCCGTG GCAGCGGCAGTGCGCAGCCCCGGCCTGGACGTACGGCCCGTGGCGCTGGTGCTCAACGGCTCCGACCCTC CGAGCCTTGTGCTGCAGCTCTGCGACCTGCTGTCGGGGCTGCGCGTGCACGGCGTGGTGTTCGAGGACGA CTCGCGCGCGCCCGCCGTCGCGCCCATTCTCGACTTCCTGTCCGCGCAGACCTCGCTGCCCATCGTAGCC GTGCACGGCGGCGCCGCGCTCGTGCTCACACCCAAGGAGAAGGGCTCCACCTTCCTGCAGCTTGGCTCCT CCACAGAGCAACAGTTGCAGGTCATTTTTGAGGTGCTGGAGGAGTACGACTGGACATCCTTTGTGGCAGT GACCACGCGTGCCCCAGGCCATCGGGCCTTCTTGTCATACATCGAGGTGCTGACTGATGGCAGCCTGGTG GGCTGGGAGCATCGAGGAGCGCTGACACTGGACCCCGGGGCGGGTGAGGCTGTACTGGGCGCACAGCTCC GTAGTGTCAGTGCGCAGATCCGCCTGCTCTTCTGCGCCCGCGAGGAGGCAGAGCCTGTTTTCCGGGCAGC GGAAGAGGCCGGCCTCACTGGGCCTGGCTACGTCTGGTTCATGGTGGGACCCCAGCTGGCTGGAGGCGGG GGCTCCGGGGTCCCTGGGGAGCCCCTTCTTCTGCCAGGAGGTTCCCCACTGCCTGCCGGGCTGTTTGCAG TGGCCTCTGCTGGCTGGCGTGACGACTTGGCACGTCGAGTGGCTGCTGGTGTGGCGGTGGTGGCCAGAGG TGCCCAGGCCCTGCTGCGAGACTATGGCTTCCTGCCTGAGCTGGGTCATGACTGCCGCACCCAGAATCGC ACCCACCGCGGGGAGAGTCTGCACAGGTACTTCATGAACATCACCTGGGATAACCGAGACTACTCCTTCA ATGAGGATGGCTTTCTGGTGAACCCTTCACTGGTGGTCATCTCCCTCACCAGAGACAGGACATGGGAAGT GGTGGGCAGCTGGGAACAGCAGACCCTCCGCCTCAAGTACCCTCTATGGTCCCGCTATGGCCGCTTCCTG CAGCCAGTGGATGACACACAGCACCTCACTGTGGCCACGCTGGAGGAGAGACCCTTTGTCATTGTAGAGC CTGCAGACCCCATCAGCGGCACTTGCATCAGAGACTCGGTCCCCTGCCGGAGCCAGCTCAACCGTACCCA CAGCCCTCCGCCTGACGCTCCCCGCCCGGAGAAGAGATGCTGCAAGGGTTTCTGCATTGATATTCTGAAG AGGCTGGCGCACACCATCGGTTTCAGCTATGACCTCTACCTGGTTACCAACGGCAAGCATGGCAAGAAGA TCGATGGCGTCTGGAATGGCATGATTGGTGAGGTGTTCTATCAGCGTGCGGACATGGCCATCGGCTCTCT CACCATCAATGAGGAGCGGTCAGAGATCGTGGACTTCTCCGTCCCCTTTGTAGAGACAGGCATCAGCGTC ATGGTGGCACGCAGCAATGGCACTGTGTCCCCCTCTGCCTTCCTCGAGCCCTACAGCCCTGCTGTCTGGG TGATGATGTTCGTCATGTGCCTCACCGTGGTCGCCGTCACAGTTTTCATCTTTGAGTACCTCAGTCCTGT GGGCTATAACCGAAGCCTGGCCACGGGCAAACGCCCTGGTGGCTCTACCTTCACCATTGGGAAATCCATC TGGCTGCTGTGGGCCCTGGTCTTCAACAACTCCGTGCCAGTGGAGAATCCTCGGGGCACCACCAGCAAGA TCATGGTGCTGGTGTGGGCCTTCTTCGCGGTCATCTTTCTTGCCAGCTACACAGCCAATCTGGCCGCCTT CATGATCCAGGAGGAGTACGTGGACACCGTGTCTGGGCTCAGCGACCGGAAGTTCCAGCGACCCCAGGAG CAATACCCACCTCTGAAGTTTGGCACGGTGCCCAATGGGTCCACGGAAAAGAACATCCGGAGCAACTACC CTGACATGCACAGCTACATGGTGCGATACAACCAGCCGAGAGTGGAGGAGGCACTCACACAGCTCAAGGC AGGGAAACTGGACGCCTTCATCTATGACGCAGCAGTGCTCAACTACATGGCCCGCAAGGATGAGGGCTGC AAGCTGGTCACCATCGGCTCCGGCAAGGTCTTCGCCACCACTGGTTATGGCATCGCCCTACACAAGGGCT CCCGCTGGAAGAGGCCCATCGACCTGGCGCTGCTGCAGTTCCTGGGGGACGATGAGATTGAGATGCTGGA GCGGCTGTGGCTTTCAGGGATCTGCCACAATGACAAAATCGAAGTGATGAGTAGCAAGCTGGATATCGAC AACATGGCAGGTGTCTTCTACATGCTCCTTGTGGCCATGGGCCTCTCCTTGCTGGTCTTCGCCTGGGAAC ATCTGGTGTACTGGCGCCTGCGGCATTGCCTGGGTCCCACCCACCGCATGGATTTCCTACTGGCCTTCTC CAGGGGTATGTACAGCTGCTGCAGCGCCGAGGCTGCTCCACCGCCGGCCAAGCCTCCGCCACCGCCTCAG CCGCTGCCCAGTCCCGCATATCCCGCCGCTCGCCCGCCCCCTGGCCCCGCACCCTTCGTGCCCCGAGAGC GCGCAGCTGCCGACCGCTGGCGCCGAGCCAAGGGCACAGGGCCCCCGGGGGGCGCAGCGATAGCCGACGG CTTCCACCGATACTACGGCCCCATCGAGCCACAGGGGCTGGGCCTCGGCGAGGCACGCGCGGCACCGAGA GGCGCAGCCGGACGCCCACTGTCCCCACCCACCACACAGCCCCCGCAGAAGCCACCACCTTCCTACTTCG CCATCGTGCGCGAGCAGGAGCCGACCGAGCCCCCGGCCGGCGCCTTCCCCGGCTTCCCATCTCCGCCTGC GCCGCCCGCTGCGGCTGCCGCTGCCGTCGGGCCGCCACTGTGTCGCCTGGCTTTCGAGGACGAAAGCCCG CCCGCACCCTCGCGCTGGCCGCGTTCAGATCCCGAGAGCCAGCCTCTGTTGGGTGGGGGCGCGGGCGGCC CGAGCGCGGGGGCCCCAACCGCACCACCGCCGCGCCGTGCCGCGCCACCACCGTGCGCCTACCTGGACCT CGAGCCTTCGCCTTCGGACTCCGAGGACTCGGAGAGCCTGGGCGGAGCATCGCTCGGTGGCCTGGAGCCC TGGTGGTTCGCCGACTTCCCCTACCCGTATGCCGAGCGCCTCGGGCCGCCGCCCGGCCGCTACTGGTCGG TCGACAAGCTCGGGGGCTGGCGCGCTGGTAGCTGGGACTACCTGCCCCCGCGCGGAGGCCCCGCGTGGCA CTGCCGCCACTGCGCCAGCCTGGAGCTGCTACCGCCACCGCGCCACCTCAGCTGCTCGCACGACGGCTTA GACGGCGGCTGGTGGGCGCCTCCGCCTCCACCCTGGGCCGCGGGGCCACCTCCCCGGCGCCGGGCGCGCT GTGGTTGTCCGCGACCTCACCCACACCGCCCCCGGGCCTCGCACCGTGCGCCCGCCGCCGCGCCGCACCA CCACCGACACAGGCGCGCGGCCGGTGGCTGGGACTTCCCGCCGCCCGCGCCCACCTCGCGTTCGCTGGAG GACCTGAGCTCCTGCCCGCGGGCGGCCCCCACGCGCAGGCTCACCGGGCCATCCCGCCACGCGCGCCGCT GCCCGCACGCTGCGCACTGGGGGCCGCCCCTGCCCACCGCATCGCACCGGAGACACCGGGGCGGGGACCT GGGCACACGCAGGGGCTCTGCGCATTTCTCCAGCCTGGAGTCCGAGGTATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_022797
Insert Size	3972 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022797.1, NP_073634.1
RefSeq Size	5246 bp
RefSeq ORF	3972 bp
Locus ID	24412
UniProt ID	Q62645
Gene Summary	N-methyl-D-aspartate receptor subunit; expressed in mid-brain structures with peak expression at day P7 [RGD, Feb 2006]

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