Ercc6 (NM_001107296) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203047 representing NM_001107296 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCATGAGGAAATCCCCAACTCCACTCAACCTCAGGGCCAAGACTGGTTACCAGGTCAGCATGTCA GTGCTGACAGAGACATACCAGCTGGGCAGGAGACTGCTGGGGACTCAGAGGCGGGGAAGTGCCTCTCCTC TCCTTCCTGTGACCATGGGCCGTCCACATCTGCTGAAGCTTGTGTACTGGCAGCCACAAGGTGGGGGCCC GCCCTGCTGCACATCGACCGGCATCAGATTCCTGCGGTGGAGCCCAGTGCTCAGGCCCTGGAGCTGCAGG GCCTGGGTGTGGACGTCTACGACCAGGCCGTCCTGGAGGAAGGTGTGCTTCAGCAGGTGGACAGTGCAAT GCATGAGGCCAGCCGTGTGGCCCAGCGTGCTGATGCGGAGAAAGAGTATCAGTCAGTCCTGGACGACCTC ATGTCATGTACAACATCCCTGAGGCAAATCAATAAAATTATAGAGCAACTTAGCCCTCAAGCTGCCAGCA ACAGAGACATCAACAGGAAACTAGATTCTGTGAAACGACAGAAGTACAACAAGGAACAACAGCTAAAGAA GATAACTGCAAAACAGAAACGTCTCCAGGCCATTCTCGGAGGAGCGGAGGTCCATGTGGAACTGGATCAT GGCAGCTTAGAGGAGGAAGATGCAGAGCCAGGCCCATCATGTCTCGGCAGTATGCTCATGCCTGCCCAGG AGAGCGCCTGGGAAGAACTTATCCGCACTGGCCAGATGACACCGTTCGGTACCCCAGCCCCTCAGAAACA GGAGAAAAAGCCTAGAAAAATAATGCTCAATGAAGCATCAGGCTTTGAAAAATATTTGGCAGACCAAGCA CAACTTTCTTTTAAAAGGAAAAAACAAGCTGCTAATAAAAGAACAGCTAAAAAAGCTGTGGTTGTATCTG AGTCAAGCCGAGGACCAAATGAAACTAAGCCAAACCAGAGAAGCCAAGCGCTCTCTCAGACAGACAAGCG CCTGAAGAAGCACATCAGGAAGCTCCAGAGGAGGGCGCTGCAGTTCCAGGGCAAAGTGGGATTGCAAAGG GGGAAGAGACCTCTGGAGCCTGAGGAGAGGCCAGGGGCAGAGGGAGACACTGAGGCCGTTCTGTCTTCCG ATGACATTAGCTATGAACTGAAGCCTCTTCGGAAAGGACAAAAATACGAGAAGAAAGTCCCAGTGCAGGA GATCGACGATGACTTCTTTCCAAGTTCTGAGGAGGAAGATGAAGCTGTGGAAGGAAGAGGAGGAGGCCGC AAGGTCTCCAGGCGCCCAGACGATGGAGATGAGGATTATTATAAACAACGATTAAGGAGGTGGAATAGGC TGAGGCTGCAGGACAAAGAGAAGCTGAAGCTTGAAGACGATTCTGAGGAGAGCGATGCTGAGTTTGACGA AGGTTTCAAAGTGCCGGGTTTTCTGTTCAAAAAGCTCTTTAAGTTCGAGGGACTCGGCCCCACTCTCATT GTCTGCCCAACGACAGTGATGCATCAGTGGGTGAAAGAATTCCACACGTGGTGGCCTCCATTCAGAGTGG CGATTCTGCACGAAACAGGCTCCTGTGCCCACAGAAAGGAGAGGCTGGTTCGAGATATTGTTCATTGTCA TGGAGTTTTGATAACTTCGTATTCCTACATTCGACTGATGCAAGACGACATTAGCAGGCATGACTGGCAC TACGTCATCTTAGACGAAGGACATAAAATTCGAAATCCAAATGCTGCAGTCACCCTTGCTTGCAAACAGA ATAACCTGCGAGAGCTGTGGTCACTCTTCGACTTTACCTTCCCAGGAAAGCTAGGCACGTTGCCTGTGTT CATGGAGCAGTTCTCTGTCCCCATCACCATGGGGGGGTACTCCAATGCTTCCCCAGTACAGGTTAAAACT GCCTATAAGTGTGCGTGTGTCTTGAGAGACACGATAAACCCGTACCTACTGCGAAGAATGAAATCAGATG TCAAGATGAGCCTTTCGCTGCCAGATAAAAATGAACAGGTCTTATTTTGCCGTCTAACTGATGAGCAGCA TAAAGTCTACCAGAATTTCATCGATTCCAAAGCAGTTTACAGGATTCTCAATGGAGAGAATCAGATTTTC TCTGGACTTGTAGCTCTGAGGAAGATCTGCAACCACCCTGACCTCTTTTCTGGCGGTCCCAAGAACACCA GTGCTCTTCCAGAGGATGGACTGGAGGAGGAGCAGTTCGGATACTGGAAACGCTCTGGGAAAATGATTGT TGTTGAGTCTTTGCTGAAAATCTGGCACAAGCAGGGTCAGCGGGTGCTGCTGTTTTCCCAGTCAAGGCAG ATGCTGCACATACTGGAAGTGTTCCTGAGAGCCCACAAGTATTCCTATCTCAAGATGGATGGGACCACTA CCATAGCATCCAGGCAGCCGCTGATTACAAAATACAATGAGGACACGTCCATCTTTGTCTTTCTTCTGAC CACACGGGTGGGCGGCTTAGGAGTGAACCTGACCGGGGCCAACAGAGTCATCATCTACGACCCTGACTGG AATCCAAGCACCGACACACAGGCACGGGAGCGAGCATGGAGGATCGGCCAGAAGAAGCAGGTGACTGTCT ACAGGCTTCTGACGGCCGGCACCATAGAGGAAAAGATCTATCACCGACAAATCTTCAAGCAATTTTTGAC AAACAGAGTGCTAAAAGACCCTAAACAAAGGCGCTTTTTCAAGTCCAATGATCTCTATGAGCTGTTTACT CTGACTAGTCCTGATGCCTCCCAGGGCACTGAAACAAGTGCTATTTTTGCAGGAACCGGATCTAATATTC AGACACTGAAATGCCATTTTAAAAAGAGGACTCCGCCAGTCCTACAAATGGATCCCAAACGCAAGAAGCC CCTGGTTTCTGACACATCTGTAAATGGTGCCACTGTGATGGAAGAAAAATCTCAGGGGTCAGGAGCTACG GGCAGTGTCCCTTCTACAGAAAGTGGCCCTTTGAAAAGTGACCGTCACACAGATGGGAGCAGAGCCAGCA GTGTTGGTTTTGGAGAAGAGGCAGATGCAGGGTCCACGCTGGAGCATCTCTCAGGGCTGAGTGGAGATTC TCCAGCAGTTGACCACACATCCAGGCTGTCAGCTGTGGCGAGCACCAGTGAGAAGCAGGGCTCTTCCTAT GAAGGGGAGTGCTGCCAGGCTCAGACGGGACCTGTTCACCTGAGTGAACAAACGGAAGGTCATTTCTCTA AGCACAAATCAAAAAGGAAACATGATGCAGCGGAGGAAGAGACCACAGAGAAGTGTCCACAGCCCAAGCA AAAGGCCAAGAACTCCAGGCATCACAGAGATGCTAAGTTTGAGGGAACTCGAGTCCCACACCTGGTGAAG AAGAGGCGGTACCATCAGCAAACCACTGAGCAGGAGAGCGGAGCCAAGGACCGGAGCAGTGATGATTATG TTTTGGAAAAGCTTTTCAAAAAATCAGTGGGCGTGCACAGCGTCGTGAAGCATGATGCCATCATAGATGG GTCCAGTCCGGATTATGTGCTGGTGGAGGCAGAAGCCAACAGAGTGGCTCAGGATGCCTTGAAAGCTCTG AGGCTCTCTCGTCAGCAGTGCCTGGGGGCAACATCTGGTGTTCCCACCTGGACTGGCCACAGGGGTATTT CCGGTGCACCAGCAGGAGTAAAAATGCGAGCACGAAACCACATGATTTTGCCGGAGCGTTTGGAGAGTGA CGGCGAGCAGCTTGCTGGGGCTGCGGCTGCGGCTGCGCCTCCCTGCAGCACAGAGCACGACGCCCTTCTG GTGGACATGAGGAACTTCATTGCTTTCCAGGCTCAGGTGGATGGCCAGGCCAGCACTCAGGAAATCCTAC AGGCGTTCGAATCCAAGCTGTCTGTGGCACAGTCCTGTGTCTTCCGAGAACTACTGAGGAATCTGTGCAA TTTCCATAGAACTCCTGGTGGTGAAGGGATTTGGAAACTGAAGCCAGAGTACTGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107296
Insert Size	3978 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107296.1, NP_001100766.1
RefSeq Size	7003 bp
RefSeq ORF	3978 bp
Locus ID	306274

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