Col5a1 (NM_134452) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203050 representing NM_134452 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTACACACCCGCTGGAAAGCTCCGCGCCCGGGCGCCCCGCTGCTGTCCTCGCCGCTGCTCCTGC TCCTGCTGCTGCTGTGGGCGCCGCCTCCGAGCCGCGCAGCTCAGCCAACAGATCTTCTGGAGATGCTAGA TTTTCACAATTTGCCCTCAGGCGTAACGAAAACCACAGGTTTCTGTGCCACTCGACGATCTTCCAAAGAG CCGGATGTTGCCTACCGAGTCTCTAAAGATGCACAGCTCAGCATGCCCACCAAGCAGCTGTACCCTGAGT CTGACTTTCCTGAGGACTTCTCCATCCTGACAACCGTGAAAGCCAAGAAAGGCAGTCAGGCCTTCCTGGT CTCCGTTTACAATGAGCAGGGCATCCAGCAGTTGGGACTGGAGCTGGGCCGCTCCCCTGTCTTCCTCTAT GAGGACCACACAGGGAAGCCCGGGCCTGAAGAGTATCCACTCTTCCCTGGCATCAACCTGTCCGATGGCA AGTGGCACCGAATCGCTATCAGTGTATACAAGAAAAATGTCACCTTGATCCTCGACTGTAAAAAGAAGAT CACGAAGTTCCTCAACCGCGGCGACCACCCCATAATAGATGTCAACGGTATCATCATGTTTGGCTCCCGG ATTCTGGATGATGAAATATTTGAGGGTGACATCCAACAGTTGCTCTTCGTCTCCGACCACCGAGCTGCCT ATGATTACTGTGAGCACTACAGCCCCGACTGTGACACAGCGGTCCCTGACACACCTCAGTCACAGGACCC TAACCCAGATGAATATTACCCAGAAGGAGAGGGTGAGACCTATTACTATGAGTACCCATATTATGAAGAC CCTGAAGACCCGGGAAAGGAGCCCGCCCCCTCTCAGAAGCCAGTGGAAGCCGCCAGAGAAACCACGGAGG TTCCTGAGGAGCAGACCCAGCCCCCATCCGAAGCCCCTACAGTGCCTGAGACCAGTGACACGGCTGGCAA GGAGGACAATCCAGGGATCGGGGACTACGACTATGTGCCCACTGACGACTACTACACTACATCCCCGTAT GAAGACTTCGGCTATGGCGAGGGCGTGGAGAACCCCGACCAGCCCACCAACCCTGACTCAGGGGCCGAGA TCCCCACCAGCACCAGTGTTACCTCCAATTCCTCCAATCCGGCTCCATCTCCAGAGGAAGGGAAGGATGA CCTGGGAGGTGAGTTCACCGAGGAGACCATCAAGAATCTAGATGAAAACTACTACGACCCGTACTTTGAC CCGGACTCCGACTCCAATGTCTCTCCATCGGAGATAGGGCCAGGCATGCCTGCTAACCAGGACACCATCT ATGAAGGGATTGGAGGACCCCGAGGTGAGAAAGGGCAAAAGGGAGAACCAGCCATCATTGAGCCGGGAAT GCTGATAGAGGGGCCCCCTGGCCCTGAAGGCCCTGCTGGTCTTCCAGGACCTCCAGGAACTACGGGTCCT ACTGGCCAAATGGGTGACCCTGGAGAAAGGGGTCCCCCTGGGCGCCCAGGTCTTCCTGGGGCTGATGGCT TGCCAGGTCCCCCCGGGACCATGCTCATGCTGCCGTTCCGTTTTGGAGGTGGTGGCGACGCCGGTTCTAA GGGCCCCATGGTCTCTGCCCAGGAATCCCAGGCACAGGCTATCCTTCAGCAAGCCAGGTTGGCACTAAGG GGACCAGCTGGTCCAATGGGTCTCACCGGGAGACCTGGCCCCATGGGTCCTCCTGGGAGTGGAGGTTTGA AGGGTGAGCCAGGAGACATGGGACCTCAGGGTCCTCGAGGTGTGCAAGGTCCACCCGGCCCAACAGGGAA GCCTGGAAGACGGGGCCGTGCTGGGAGTGATGGTGCCAGAGGCATGCCTGGACAAACAGGCCCCAAGGGT GACCGTGGCTTTGATGGTCTGGCTGGGTTGCCGGGAGAGAAAGGCCATAGAGGTGACCCTGGTCCTTCTG GCCCGCCGGGACTCCCAGGAGATGATGGAGAAAGGGGTGACGATGGAGAAGTTGGGCCCAGGGGGCTGCC TGGGGAGCCTGGACCACGTGGTCTGCTTGGGCCAAAAGGCCCCCCTGGCCCACCTGGACCTCCTGGTGTG ACGGGTATGGATGGCCAGCCTGGCCCCAAAGGAAATGTGGGTCCCCAGGGAGAGCCTGGGCCCCCAGGAC AGCAGGGTAATCCTGGTGCCCAGGGTCTTCCCGGTCCCCAGGGTGCCATTGGTCCTCCAGGAGAAAAGGG TCCTTTGGGGAAACCAGGTCTCCCAGGAATGCCCGGAGCTGATGGACCCCCGGGGCACCCTGGCAAAGAA GGTCCTCCAGGAGAGAAAGGAGGCCAGGGTCCTCCCGGCCCCCAGGGTCCCATTGGCTACCCAGGTCCAC GAGGAGTCAAGGGGGCAGATGGCATCCGAGGGCTGAAGGGCACCAAGGGAGAGAAGGGTGAAGACGGCTT CCCTGGGTTTAAAGGCGACATGGGAATAAAGGGTGACCGGGGGGAGATCGGCCCACCCGGCCCCCGAGGA GAAGATGGTCCTGAAGGCCCAAAAGGTCGAGGTGGTCCCAATGGTGATCCTGGTCCCCTGGGGCCCACCG GGGAGAAGGGGAAGCTCGGAGTGCCAGGATTACCAGGGTACCCAGGAAGACAAGGGCCAAAGGGTTCTAT TGGATTCCCTGGCTTCCCGGGCGCCAACGGAGAGAAAGGTGGCAGGGGGACACCTGGAAAGCCAGGACCA CGGGGACAGCGAGGCCCAACGGGCCCGAGGGGTGAACGAGGCCCACGGGGCATCACTGGGAAGCCTGGCC CTAAGGGCAACTCAGGAGGTGATGGCCCAGCCGGCCCTCCTGGTGAGCGGGGACCCAATGGACCCCAAGG CCCCACCGGCTTCCCTGGACCTAAGGGTCCCCCGGGTCCACCAGGCAAGGACGGGCTCCCCGGACATCCT GGGCAGAGAGGGGAGACTGGTTTCCAAGGCAAGACTGGCCCTCCAGGCCCTCCAGGAGTGGTTGGCCCTC AGGGTCCCACAGGCGAGACGGGTCCCATGGGGGAGCGTGGCCACCCTGGTCCTCCAGGCCCCCCTGGTGA ACAGGGCCTCCCAGGTGCTGCTGGGAAAGAAGGGACGAAGGGTGACCCAGGTCCTGCTGGCCTCCCTGGG AAGGATGGACCTCCAGGATTGCGTGGATTCCCTGGGGACCGAGGGCTCCCCGGCCCCGTGGGAGCACTCG GACTGAAAGGCAGCGAAGGCCCCCCTGGCCCCCCAGGTCCTGCGGGTTCTCCAGGGGAGAGAGGACCAGC TGGTGCTGCTGGACCCATTGGAATTCCAGGGAGACCTGGGCCTCAGGGACCTCCGGGGCCTGCTGGAGAG AAAGGGGTTCCTGGAGAGGAAGGTCCGCAAGGCCCGGCTGGCCGAGATGGCCTGCAAGGTCCCGTGGGGC TCCCTGGACCAGCCGGCCCCGTGGGTCCTCCTGGAGAAGATGGAGATAAGGGAGAGATCGGAGAGCCCGG GCAGAAAGGGAGCAAGGGCGACAAAGGCGAGCAGGGTCCTCCTGGGCCTACCGGTCCTCAAGGCCCCACT GGGCAGCCAGGCCCTTCGGGAGCAGATGGTGAACCTGGCCCTCGTGGACAGCAGGGCCTGTTTGGGCAGA AAGGAGATGAAGGTTCAAGAGGTTTCCCAGGACCGCCAGGGCCAGTGGGCTTGCAGGGTTTACCAGGACC TCCAGGAGAAAAGGGTGAGACAGGAGACGTGGGCCAGATGGGCCCTCCTGGTCCACCAGGCCCCCGAGGA CCCTCTGGAGCTCCAGGAGCTGATGGGCCACAGGGTCCTCCAGGAGGGATTGGCAACCCTGGTGCAGTCG GAGAAAAGGGAGAGCCTGGCGAAGCTGGAGAGCCTGGCCTTCCAGGAGAAGGAGGTCCCCTGGGACCTAA AGGAGAAAGAGGGGAGAAGGGAGAGGCTGGCCCCTCTGGTGCTGCTGGACCCCCTGGACCCAAAGGCCCA CCTGGAGATGATGGCCCCAAAGGCAGCCCCGGCCCCGTGGGCTTTCCTGGAGACCCTGGGCCCCCTGGAG AGCCAGGCCCAGCAGGTCAAGATGGCCCACCTGGTGACAAAGGGGATGATGGAGAACCTGGGCAGACGGG ATCCCCAGGCCCTACTGGTGAACCTGGTCCATCTGGGCCTCCAGGAAAGAGGGGTCCACCGGGCCCTGCA GGCCCTGAAGGCAGGCAGGGGGAGAAAGGAGCCAAGGGAGAAGCTGGCTTAGAAGGCCCTCCTGGGAAGA CTGGCCCCATTGGTCCCCAAGGGGCCCCTGGGAAGCCTGGCCCTGATGGTCTTCGTGGGATCCCTGGTCC TGTGGGTGAGCAAGGCCTCCCAGGATCCCCAGGCCCTGATGGTCCACCCGGCCCCATGGGTCCTCCAGGA CTCCCTGGCCTCAAAGGAGACTCCGGTCCCAAAGGTGAAAAGGGCCATCCGGGCCTGATTGGACTCATCG GACCTCCGGGCGAACAAGGTGAAAAAGGTGACCGAGGACTCCCAGGCCCCCAGGGCTCATCTGGTCCTAA AGGAGAACAGGGCATTACAGGTCCTTCTGGCCCGCTTGGGCCTCCTGGTCCCCCAGGCTTGCCGGGCCCT CCAGGTCCCAAAGGTGCTAAAGGTTCATCGGGTCCCACTGGCCCGAAGGGCGAGGCAGGCCATCCAGGAC TCCCTGGTCCACCCGGGCCTCCAGGCGAGGTCATCCAGCCCCTGCCAATCCAGGCGTCCAGGACTCGGCG GAACATTGATGCCAGTCAGCTCCTGGATGATGGGGCTGGGGAGAGCTACGTGGATTATGCAGATGGCATG GAAGAGATCTTTGGTTCCCTCAACTCCCTGAAGCTGGAGATTGAGCAGATGAAGCGGCCACTGGGCACAC AGCAGAACCCAGCCCGTACCTGCAAGGACCTTCAACTCTGCCATCCTGATTTCCCAGACGGCGAATACTG GGTCGATCCCAACCAAGGGTGCTCCAGGGACTCCTTCAAAGTCTACTGCAATTTCACAGCTGGAGGGTCC ACGTGCGTCTTCCCTGACAAGAAGTCTGAGGGAGCCAGAATCACTTCTTGGCCCAAAGAAAACCCAGGTT CCTGGTTCAGTGAATTCAAGCGTGGGAAACTGCTCTCCTATGTGGATGCTGAAGGCAACCCTGTGGGCGT GGTACAAATGACCTTCCTGCGGCTGCTGAGTGCCTCTGCCCAGCAGAACATCACCTACAACTGCTACCAG TCCGTGGCCTGGCAGGATGCCGCCACAGGCAGCTACGATAAGGCTATCCGCTTCTTGGGCTCCAACGATG AGGAAATGTCTTATGATAACAACCCCTACATCCGTGCCCTGGTGGATGGCTGTGCTACCAAGAAAGGCTA CCAGAAGACGGTGCTGGAGATTGACACGCCCAAAGTTGAGCAAGTCCCCATTGTGGACATCATGTTCACC GACTTTGGTGAAGCCTCACAGAAATTTGGATTTGAAGTAGGGCCAGCTTGCTTCCTAGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_134452
Insert Size	5523 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_134452.1, NP_604447.1
RefSeq Size	5551 bp
RefSeq ORF	5523 bp
Locus ID	85490
UniProt ID	Q9JI03
Gene Summary	alpha chain of type V collagen; binds heparin with high affinity [RGD, Feb 2006]

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