Spag5 (NM_001044224) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Deepest
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203082 representing NM_001044224 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGGGTGAAAACACTGAATCTCGGCTTGTCGCCTTCGCCCCAATCGGGGAAACCAGCTATGAGAA CTCCTCTCCGAGAGCTTAAACTACAGCCCGAGGCCCTCGCTGATTCAGGGAAAGGTCCTATGTTCTCCTC GCTGACCCCATACCTGCGCAGGCTGGATCTGAAGGAAAGGCGCAACAGCTCATCTCCAGTGAATTTTGTT AATGCAGAGAACTCATCTCCAGTGGATTTTGTCAATACTGAGAACACATCTCCAGTGGATTTTGACAATA CTGAGAACAACTTCCTTTCAGAACAGTTCAGCCATCCTTCAGAGTACATAGAAGCTTGTCAGCGTGAATC AGATCTAACTCCTGAAAGCAGCTCTCCTTTCAACACACTGGAGAAGGCAGTGGAAGCAATGGATGATTTT GTGGCAGAACCAATAGATGACGGTATAGTTAAGACCATGGTTCTTTTACCCTTTTCCTTAGGGCAGCAGC AAGACCTGATGCTCCAGGCTCACTTAGATACCACAGCAGAGAGAAATAAAAGCTCTCTAAAAGAATCTTT GGGGCTAGAAGACCTGGTGGAAAAGGAGGCGGCACCTTGTGTGGAAGACAGCTTACCAGAAACTGTTGCT ATCAGGCCTGAGCAACCCATCTCTCAGGACCCTCCATTAAGAGACAGTAACACTGAAGCTGCACCTGCGG GCTCAGTTCCTTCTGAAAATGTCTTGGGTTTCACTGTGGCTTGTCTCTCTCCCTCAACTGTCTTGACCAA AGATTTCTCTGTCGATCATGTCGATCCTGGGGAGGAAACTGCAGAGAACAGAGTTATACAGGAAGTGGAA AAGAGTTTTCTCGCACTCCCTGAGGAGGCTGGATTGGGAGATCAGGCACTTGCTGTAAATGCAGAAGACG TCTCATCCTTATACCTGTCATCAAGTCTAGTAGAAATGGGACCCCAGGAGGCTCCAGGCCCAACAGCAGA AGATGCCAGTAGGATTCCTGACTTGGAGTCAGAGACTTGGATGTCCCCATTGGCCTGGCTAGAAAAAGGT GTGAACACGTCAGTCATGCTACAAAGTCTCCGCCAGAGTTTACCCTTTTCAAAATCTGTGCTTCAGGATG CTGCCGTTGGCAATACACCCCACTCCACTTATTCTGTGGGAACTTCCTTTACTCCTCCAGTGCCACTGGA AGTAGGCACCAAGGACAGTACTTCAGAGACAGAGCGCCTCCTCTTGGGCTGCCGTCCTCCAGATCTGGCA GCCTTGTCCCGGCACGACTTGGAAGAGAACCTGTTGAACGCCCTTGTCTTGCTGGAAGTTCTTTCTCACC AGCTGCAAGCCTGGAAGAGTCAGCTGACTGTCCCGAGCCGGCAAGCTCAAGACAGCAGCACACAGACCGA CAGCTCTGCAGCTGTGGTCACTAAGACACCTAAACATCTTCAGGACAGCAAGGAGATTCGACAGGCTCTA CTGCAAGCCAGGAATGTCATACATTCATGGGGGCTAGTCTCTAGAGACCTGATGTCCTTGCTTCACCTGT CCCTGACACATGTGCAAGATGACAGAGTGACAGTGAGTCAGGAGTCTCAGCGTGCAGAGACCCTGGTTTC CTCCTGTTCTCGTGTACTGAAGAAATTGAGGGCAAAGCTCCAGAGCCTCAAAACAGAATGGGAGGAGGCA CGGCACAGGGAGGAAATGGCCCTTAAAGGCAAGGATGCGGCAGAGGCAGTACTGGAGGCTTTCCGTGCAC ACGCTAGCCAGCGCATCAGCCAACTGGAACAGGGTATCACATCTGTGCAGGAATTCAGAGGCCTTCTACA GGAGGCACAGACCCAACTGATAGGGCTTCACACAGAGCAAGAAGAGCTGGCTCAGCACACAGTGAGTCTG ACTTCTGCCTTGCAGCAGGACTGGACATCAGTCCAACAGAATTATGGGACATGGGCAGCTTTGCTGAGTC GGTCTCGAGAACTCACCAAGAAACTCACAGCCAAGAGCCGGCAAGCCCTTCAGGAACGTGATGCTGCAAT TGAGGAAAAGGATCAGGTTGTGAAGGAAGTGGAACAAGTCTCTGCCCATTTAGAGGACTGTAAAGGCCAA ATAGAACAACTGAAGTTGGAAAATAGTCGCCTTACTGCAGATCTCTCGGCTCAGCTGCAGACTCTGGCTA GCACAGAGAACGAACTAAAGGAACTACAGAGTCAGCATTCCCATTGTGTCCAAGACTTGGCCATGAAGGA TGAACTGCTGTGTCAACTTACCCAGAGCAACAAGGAACAGGCTGCTCAGTGGCAAAAGGAAGAAACGGAA CTGAAACACAGACAAGCAGAACTGCTGCAGCAGAAGGCTGTGTTGGCTAAGGAGGTACAGGACCTGAGGG AGACCATGGAGTTTGTAGATGAAGAGAGTCAAGTTGCTCACCTGGAGCTGGGCCAGATCGAGAGTCAGTT GAAAGCCACACTAGAAGTTCTGCGGGAGCGCAGCCTGCAGTGTGAGACCCTCAAGGACACCGTGGAGAGC CTGAGGGCAGAGCTGGCCAGCACCGAAGCAAAGCATGAGCAACAAGCGCTGGAGAAGACGCACCAGCATT CTAAAGAGCTGTGTCTATTGGCGGAGCAGCTACAGAGTCTCACCCTCTTCTTAGAGGCTAAACTAGAGGA GAACAAGGCAGAATCAGACATCATTCTGCCCAGCACAGGCTGTGCTCCAGCCCAGGAACACCCTCCTTCC AGTGATAGCAGCGTCTCAGAACAGATCCCGACAGCAGTGGTAGATGAAGTGCCAGAACCAGCTCCTGTGC CATTGCTTGGAAGTGTTAAGAGTGCTTTCACCCGCGTAGCCTCAACAGCTCCCTTTCGGCCTACAGAGAC CCCAGCCCTGGAGAAGAGCCTGGCAGAAATGAGTGCTGTGTTACAGGAGCTTCAACGCCTCTGTGCCCTG CTGCAAGAATCTAAAGAGGAAGCCGTTGGTGTCCTGCAGAGGGAAATCTGTGAACTGCACACAAGACTAC AGGCCCAAGAAGAAGAACATCAGGAAGCCCAAAAGGCAAAGGAAGCAGACATAGAGAAGTTGAACCAGGC CTTGTGCTTGCGCCACAAGAATGAGAAGGAACTCCTGGAAGTGATACAGAAGCAGAACGAAAAGATCCTG GGGCAGATAGACACGAGTGGCCAGCTCATAAACCTCAGGGAGGAGGTGACCCAGCTTACACGTTTACTTC GGCGGGCAGAGACAGAGACTAAAGTGCTCCAGGAAGCCCTGGAAGGCCAGGTAGATCCCAGCTGTCAGCT GATGGCTACTAACTGGATCCAGGAAAAAGTGTTTCTCTCGCAGGAGGTGAACAAACTGAGGGCTATGTTC CTGGAGGTGAAAAATGAGAAGAAACAGCTGATGGACAAGTATCTGAGCCATAGGCACATCCTGGAGGAGA ATCTTCGGCGCTCTGACACAGAGTTAAAGAAACTTGATGACACAATTCAGCATATCTATGAGACTCTGTT GTCTATCCCAGAGGTAGTGAAGAGTTGCAAGGAGCTACAAGGATTGCTAGAATTTCTGAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001044224
Insert Size	3564 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001044224.1, NP_001037689.1
RefSeq Size	3881 bp
RefSeq ORF	3564 bp
Locus ID	252918
Gene Summary	C-terminal has two leucine zippers which are involved in sperm tail protein interactions [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.