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Wt1 (NM_031534) Rat Untagged Clone
CAT＃: RN203084
Wt1 (untagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug)
CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN203084 representing NM_031534
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTCCGACGTGCGGGACCTGAACGCGCTGCTGCCCGCCGTGTCCTCGCTGGGCGGTGGAGGCGGCT GTGGGCTCCCTGTGAGCGGCGCGGCGCAATGGGCGCCGGTGCTGGACTTCGCGCCTCCGGGCGCCTCGGC TTACGGGTCACTGGGCGGTCCCGCGCCTCCTCCCGCTCCGCCGCCACCTCCGCCGCCACCCCACTCCTTC ATCAAACAGGAGCCCAGCTGGGGCGGCGCCGAGCCGCACGAGGAGCAGTGCCTGAGCGCCTTCACCTTGC ACTTCTCCGGCCAGTTCACCGGTACAGCCGGGGCCTGTCGCTACGGACCCTTCGGTCCTCCCCCGCCCAG CCAGGCGTCATCCGGCCAGGCCAGGATGTTCCCCAATGCGCCCTACCTGCCCAGCTGCCTGGAGAGCCAG CCCTCCATCCGCAACCAAGGATACAGCACGGTCACTTTCGACGGGGCACCCAGTTATGGCCACACGCCCT CGCACCATGCAGCGCAGTTCCCCAATCATTCCTTCAAACACGAGGACCCCATGGGCCAGCAGGGCTCGCT GGGCGAGCAGCAGTACTCGGTGCCACCTCCAGTGTATGGCTGCCACACCCCTACCGACAGTTGCACAGGC AGCCAGGCCCTGCTCCTGAGGACGCCCTACAGCAGTGACAATTTATACCAGATGACCTCCCAGCTTGAGT GCATGACCTGGAACCAGATGAACCTCGGAGCTACCTTGAAGGGAATGGCTGCTGGGAGCTCCAGCTCAGT GAAATGGACAGAAGGGCAGAGCAACCACGGCACAGGGTACGAGAGTGAGAACCACACAACCCCCATCCTC TGTGGTGCCCAGTACAGAATACACACCCACGGTGTCTTCCGAGGCATTCAGGATGTGCGGCGCGTATCTG GAGTGGCCCCAACTCTTGTCCGGTCAGCATCTGAAACCAGTGAGAAGCGTCCTTTCATGTGTGCGTACCC AGGCTGCAATAAGAGATATTTTAAGCTGTCCCACTTACAGATGCACAGCCGGAAGCACACTGGTGAGAAA CCATACCAGTGTGACTTCAAGGACTGCGAGAGAAGGTTTTCTCGCTCAGACCAGCTCAAAAGGCACCAAA GGAGACACACAGGTGTGAAACCATTCCAGTGTAAAACTTGTCAGCGAAAGTTTTCCCGGTCCGACCACCT GAAGACCCACACCAGGACTCATACAGGTAAAACAAGTGAAAAGCCCTTCAGCTGTCGGTGGCACAGTTGT CAGAAAAAGTTTGCGCGGTCAGACGAATTAGTCCGCCATCACAACATGCACCAGAGAAACATGACCAAAC TCCAGCTGGCGCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_031534 |
| Insert Size | 1347 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031534.2, NP_113722.2 |
| RefSeq Size | 2645 bp |
| RefSeq ORF | 1347 bp |
| Locus ID | 24883 |
| UniProt ID | P49952 |
| Gene Summary | This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the human gene is mutated in a small subset of patients with Wilm's tumors. Authors of PMID:7926762 provide evidence that Wt1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR203084 | Wt1 (Myc-DDK-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug) |
CNY 4,024.00 |
|
| RR203084L1 | Lenti ORF clone of Wt1 (Myc-DDK-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug) |
CNY 6,424.00 |
|
| RR203084L2 | Lenti ORF clone of Wt1 (mGFP-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug) |
CNY 8,460.00 |
|
| RR203084L3 | Lenti ORF clone of Wt1 (Myc-DDK-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug) |
CNY 7,600.00 |
|
| RR203084L4 | Lenti ORF clone of Wt1 (mGFP-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug) |
CNY 8,460.00 |
