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Mapk10 (NM_012806) Rat Untagged Clone
CAT＃: RN203224
Mapk10 (untagged ORF) - Rat mitogen activated protein kinase 10 (Mapk10), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Jnk3; SAPb; SAPKC; Serk2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN203224 representing NM_012806
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAAAAGCAAGGTAGATAACCAGTTCTACAGTGTGGAAGTGGGAGACTCAACCTTCACAGTTCTAA AGCGCTACCAGAACCTGAAGCCGATCGGCTCTGGGGCTCAGGGAATAGTTTGTGCTGCGTATGACGCTGT CCTCGACAGAAATGTGGCCATTAAGAAGCTCAGCAGACCCTTCCAGAACCAAACTCATGCCAAGAGGGCT TACCGGGAGCTGGTCCTCATGAAGTGTGTGAACCATAAAAACATTATTAGCTTATTAAATGTCTTTACAC CCCAGAAAACACTGGAGGAGTTCCAAGATGTTTACTTAGTGATGGAACTGATGGACGCCAACTTGTGTCA GGTGATTCAGATGGAGCTGGACCACGAGCGGATGTCGTACTTGCTGTACCAGATGCTGTGCGGCATCAAA CACCTCCACTCCGCTGGGATCATCCACAGGGACTTAAAACCCAGTAACATCGTAGTCAAGTCTGATTGCA CACTGAAAATCCTGGACTTTGGACTGGCCAGGACAGCGGGCACAAGCTTCATGATGACTCCGTATGTGGT GACGAGATATTACAGAGCCCCCGAGGTCATCCTGGGCATGGGCTACAAGGAGAACGTGGACATATGGTCT GTGGGCTGCATCATGGGAGAAATGGTTCGTCACAAAATCCTCTTTCCCGGAAGGGACTATATTGACCAGT GGAACAAAGTCATAGAGCAGCTAGGAACTCCGTGTCCAGAATTCATGAAGAAATTGCAGCCCACCGTCAG AAACTACGTGGAGAACCGGCCCAAGTATGCAGGCCTCACCTTCCCCAAGCTCTTTCCAGATTCCCTCTTC CCAGCGGATTCCGAGCACAATAAACTTAAAGCCAGCCAAGCCAGGGACTTGTTGTCAAAGATGTTAGTGA TTGACCCAGCGAAGAGGATATCGGTGGATGACGCATTGCAGCATCCGTACATCAACGTTTGGTACGACCC TGCTGAAGTGGAGGCGCCTCCGCCTCAGATATATGACAAGCAACTGGATGAAAGGGAGCACACCATCGAA GAATGGAAAGAACTCATCTACAAGGAAGTAATGAACTCAGAAGAGAAGACTAAGAACGGCGTAGTCAAAG GCCAGCCCTCACCTTCAGGTGCAGCAGTGAACAGCAGTGAGAGTCTCCCTCCATCCTCGTCTGTCAACGA CATCTCCTCCATGTCCACCGACCAGACCCTCGCATCCGACACTGACAGCAGCCTGGAAGCCTCGGCGGGA CCGCTGGGTTGTTGCAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_012806 |
| Insert Size | 1281 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012806.2, NP_036938.2 |
| RefSeq Size | 2786 bp |
| RefSeq ORF | 1281 bp |
| Locus ID | 25272 |
| UniProt ID | P49187 |
| Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017] Transcript Variant: This variant (2) uses an alternate acceptor splice site in the 5' region, which results in translation initiation from an in-frame, downstream start codon compared to variant 1. The encoded isoform (2, also known as SAPK beta) has a shorter N-terminus compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR203224 | Mapk10 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase 10 (Mapk10), (10 ug) |
CNY 3,656.00 |
|
| RR203224L3 | Lenti ORF clone of Mapk10 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase 10 (Mapk10), (10 ug) |
CNY 6,080.00 |
|
| RR203224L4 | Lenti ORF clone of Mapk10 (mGFP-tagged ORF) - Rat mitogen activated protein kinase 10 (Mapk10), (10 ug) |
CNY 6,650.00 |
