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Aco1 (NM_017321) Rat Untagged Clone
CAT＃: RN203228
Aco1 (untagged ORF) - Rat aconitase 1, soluble (Aco1), (10 ug)
CNY 11,500.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Acon1; AH; IRP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN203228 representing NM_017321
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAATCCATTTGCGCACCTTGCCGAGCCCTTGGACCCTGCACAGCCAGGAAAGAAATTCTTCAACT TGAATAAATTGGAGGACTCAAGATATGGACGCTTACCGTTCTCTATCAGAGTTCTCCTGGAGGCCGCCGT TCGGAACTGTGACGAGTTTTTGGTGAAGAAGAATGACATTGAGAATATCCTGAACTGGAGTATCATGCAG CATAAGAGCATAGAAGTGCCGTTTAAGCCAGCCCGAGTCATACTGCAGGACTTTACGGGCGTGCCTGCTG TGGTTGATTTTGCAGCAATGCGCGATGCTGTGAAGAAGTTGGGAGGAAATCCAGAGAAAATAAACCCTGT CTGCCCCGCTGACCTTGTAATCGATCATTCCATCCAGGTTCATTTCAACAGAAGGGCAGACAGTTTGCAG AAGAATCAAGACCTGGAATTTGAAAGGAATAGAGAACGATTTGAATTTTTAAAGTGGGGTTCCCAGGCCT TTTGCAACATGAGGATTATTCCTCCTGGCTCAGGAATTATTCACCAAGTGAATCTGGAGTATTTGGCAAG AGTAGTGTTTGATCAGGATGGATGTTACTACCCAGATAGCCTCGTGGGCACAGATTCTCACACCACCATG ATTGATGGTCTGGGAGTTCTTGGTTGGGGTGTAGGTGGTATTGAAGCAGAAGCTGTCATGCTGGGTCAGC CAATCAGCATGGTGCTTCCCCAGGTGATTGGCTACAAGCTGATGGGGAAGCCTCACCCTCTGGTAACCTC CACGGACATTGTGCTCACCATTACCAAGCACCTCCGACAAGTTGGGGTCGTGGGCAAATTCGTGGAGTTT TTCGGGCCAGGAGTGGCCCAGCTGTCCATTGCTGACCGAGCTACGATTGCCAACATGTGCCCAGAGTACG GCGCGACGGCAGCCTTCTTCCCGGTCGACGACGTTAGCATCGCGTACCTGGTGCAGACAGGTCGTGAGGA AGACAAAGTAAAGCACATTAAAAGGTATCTTCAGGCTGTAGGCATGTTTCGAGACTTCAGTGACTCCTCT CAAGACCCAGACTTCACTCAGGTCGTGGAGTTAGATTTGAAAACAGTTGTGCCTTGCTGCAGTGGACCTA AAAGACCTCAGGACAAAGTTGCTGTGTCTGAGATTGAAAAGGACTTTGAAAGCTGCCTTGGAGCCAAGCA AGGATTTAAAGGTTTTCAAGTTGCTCCAGACCATCACAATGACCACAAGACGTTTATCTATAACGACAGT GAATTCACTCTTGCTCATGGCTCCGTGGTGATCGCCGCCATCACTAGCTGCACAAACACCAGCAATCCGT CCGTGATGTTAGGCGCAGGATTGTTAGCAAAGAAAGCCGTAGAGGCTGGCCTGAATGTGAAGCCTTACGT CAAAACCAGCCTGTCTCCTGGGAGTGGAGTGGTCACCTACTACCTTCGAGAGAGTGGAGTCATGCCTTAC CTGTCCCAGTTAGGGTTTGACGTGGTGGGCTACGGCTGCATGACCTGCATCGGCAACAGTGGACCCCTCC CCGAACCTGTGGTGGAGGCTATCACCCAGGGAGACCTTGTGGCTGTTGGGGTACTGTCTGGAAACAGGAA TTTTGAAGGACGAGTCCATCCTAACACCCGGGCCAACTACTTAGCATCTCCCCCACTAGTAATAGCATAT GCAATTGCAGGCACCGTCAGGATCGACTTCGAGAAAGAGCCTTTGGGAGTGAACGCACAGGGCCAACAAG TGTTTCTGAAGGATATCTGGCCAACTCGAGATGAGATCCAGGAGGTGGAGCGGAAGTATGTCATCCCCGG CATGTTCAAGGAGGTCTATCAGAAGATAGAGACTGTAAACAAAAGCTGGAATGCCTTAGCAGCCCCTTCA GAGAAGCTGTATGCGTGGAACCCCAAGTCTACTTATATCAAGTCACCGCCATTCTTTGAAAGCTTGACTT TAGATCTCCAGCCCCCCAAGTCTATAGTGGATGCCTATGTGCTACTAAATCTAGGAGATTCCGTAACAAC GGACCATATCTCTCCAGCGGGGAACATTGCAAGAAACAGCCCTGCCGCTCGCTACTTGACGAACAGAGGC CTGACGCCACGAGATTTCAACTCCTACGGCTCCCGCCGGGGTAACGACGCCATCATGGCACGGGGGACAT TTGCCAACATTCGCTTGCTGAACAAGTTTCTGAACAAGCAGGCCCCTCAGACTGTCCACCTTCCTTCAGG AGAAACCCTCGATGTGTTCGATGCCGCTGAGCGGTACCAGCAGGCTGGACTTCCCCTGATTGTTCTGGCT GGCAAAGAGTACGGTTCAGGCAGCTCCCGAGACTGGGCAGCCAAAGGTCCTTTCCTGCTGGGAATCAAAG CTGTCCTGGCAGAGAGCTACGAGCGCACTCACTGCAGCAACCTGGTTGGCATGGGGGTGATCCCCCTTGA GTATCTCCCCGGCGAAACTGCAGACTCTCTGGGACTCACGGGTCGGGAAAGGTACACGATCCACATTCCC GAACACCTTAAGCCCCGCATGAAGGTTCAGATAAAGCTGGACACCGGGAAGACCTTCCAGGCCGTGATGA GGTTCGACACCGACGTGGAGCTCACTTACTTCCACAATGGAGGCATCCTGAACTACATGATCCGAAAGAT GGCCCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017321 |
| Insert Size | 2670 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017321.1, NP_059017.1 |
| RefSeq Size | 3564 bp |
| RefSeq ORF | 2670 bp |
| Locus ID | 50655 |
| UniProt ID | Q63270 |
| Gene Summary | This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism [provided by RefSeq, Jan 2014] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR203228 | Aco1 (Myc-DDK-tagged ORF) - Rat aconitase 1, soluble (Aco1), (10 ug) |
CNY 11,500.00 |
|
| RR203228L3 | Lenti ORF clone of Aco1 (Myc-DDK-tagged ORF) - Rat aconitase 1, soluble (Aco1), (10 ug) |
CNY 13,210.00 |
|
| RR203228L4 | Lenti ORF clone of Aco1 (mGFP-tagged ORF) - Rat aconitase 1, soluble (Aco1), (10 ug) |
CNY 14,350.00 |
