Mrc1 (NM_001106123) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203299 representing NM_001106123 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACTCCCCCTGCTCCTGGCTTTTATCTCTGTGATTCCGGTCGCTGTTCAACTCTTGGACTCACGGC AATTTTTAATCTATAATGAGGACCACAAGCGCTGCGTGGACGCTCTAAGCGCCATCTCCGTTCAGACGGC AACTTGCAACCCGGAAGCTGAATCCCAGAAATTCCGCTGGGTGTCGGAGTCGCAGATCATGAGTGTTGCT TTCAAATTGTGTTTGGGGGTGCCATCGAAAACTGACTGGGCCTCCGTCACCCTGTATGCCTGCGACTCGA AAAGTGAATTTCAGAAATGGGAGTGTAAAAACGACACACTCTTTGGAATCAAGGGCACAGAGCTATATTT TAACTATGGCAACAGGCAAGAAAAGAATATCAAGCTTTACAAAGGTTCCGGTTTGTGGAGCAGATGGAAG GTCTACGGGACCACAGATGACCTGTGCTCTAGGGGATATGAAGCCATGTACTCCTTACTGGGCAATGCAA ACGGAGCTGTCTGTGCATTTCCATTCAAGTTTGAAAACAAGTGGTACGCAGACTGCACCTCTGCCGGGCG GTCAGATGGATGGCTCTGGTGTGGAACCACCACTGACTATGACACCGACAAGCTGTTTGGATTTTGTCCA CTGCAGTTTGAGGGCAGCAAGAGGCTGTGGAATACAGACCCACTGACTGGCATTCTTTACCAGATAAACT CCAAGTCTGCCTTAACCTGGCACCAGGCAAGAGCAAGCTGCAAGCAGCAGAATGCTGAGCTCCTGAGTGT CACGGAGATTCACGAGCAGATGTACCTCACAGGATTGACCAGTTCCTTGACCTCTGGACTCTGGATTGGA CTCAACAGTTTAAGTTTAAGCACTGGCTGGCAGTGGGCTGGGGGAAGCCCATTCCGGTATCTGAACTGGC TACCAGGAAGTCCATCATCAGAACCTGGAAAGAGCTGTGTGTCACTAAACCCTGGAAAAAATGCCAAGTG GGAGAATCTGGAATGTGTTCAGAAACTTGGCTACATTTGTAAAAAAGGCAACAATACTTTGAATCCATTT ATCATTCCCTCGGAAAGTGATGTGCCTACTGCCTGCCCTAATCAGTGGTGGCCCTATGCAGGCCACTGCT ACAGGATCTACAGGGAAGAGAAGAAGATCCAGAAATACGCTTTGCAAGCCTGTAGGAAGGAGGGTGGTGA CCTGGCAAGTATCCACAGCATTGAGGAGTTTGACTTCATCTTCTCCCAGCTAGGATACGAGCCAAATGAT GAGCTGTGGATTGGTTTAAATGACATCAAGATTCAGATGTACTTTGAGTGGAGCGATGGGACCCCGGTGA CATTTACTAAATGGCTTCCCGGAGAGCCGAGCCATGAGAACAACAGGCAGGAGGACTGCGTGGTGATGAA AGGGAAGGATGGATACTGGGCAGATAGAGCTTGTGAGCAACCACTGGGTTACATCTGTAAGATGGTCTCA CAAATCCACACTGTAATACCAGAGGGTGCAGAAAAAGGCTGCCGGAAAGGCTGGAAACGGCATGGGTTTT ACTGCTACTTGATTGGATCCACCCTTTCAACGTTCGCTGATGCAAACCAAACATGCACAAATGAGAAGGC TTATTTGACAACAGTTGAAGACAGATATGAACAAGCATTCCTGACTAGTTTGGTTGGATTGAGGCCTGAA AAATATTTTTGGACAGGACTCTCAGATGTTCAAAACAAAGGGACGTTTCGGTGGACTGTGGACGAGCAGG TGCAGTTTACACACTGGAACGCTGACATGCCAGGACGAAAGGCAGGATGCGTTGCTATGAAAACTGGAGT GGCAGGTGGTTTATGGGATGTTTTGAGTTGTGAAGAAAAGGCAAAATTTGTGTGCAAACACTGGGCAGAA GGAGTAACTCGTCCACCAGAGCCCACAACAACTCCTGAACCCAAATGTCCAGAAGACTGGGGTACCACCA GTAAAACCAGCATGTGTTTCAAGCTGTATGCAAAAGGAAAACATGAAAAGAAAACGTGGTTTGAGTCTCG AGATTTTTGCAAAGCTATTGGGGGAGAGCTGGCGAGCATCAAGAGTAAAGATGAACAGCAAGTGATCTGG AGGCTGATTACGAGCAGTGGAAGCTACCATGAGCTGTTTTGGCTGGGACTGACCTATGGAAGTCCTTCAG AGGGGTTCACCTGGAGTGATGGTTCTCCTGTTTCCTATGAAAATTGGGCTTATGGAGAGCCAAATAATTA CCAAAACGTTGAATATTGTGGTGAGTTGAAAGGTGACCCCGGCATGTCCTGGAATGATATTAACTGTGAA CACCTCAACAACTGGATCTGTCAGATCCAAAAAGGGAAAACACTACTACCCGAGCCTACACCCGCTCCTC AAGACAATCCACCAGTGACTGCAGATGGGTGGGTTATTTACAAAGACTACCAGTACTATTTCAGCAAGGA GAAGGAAACCATGGACAACGCGCGGGCCTTCTGCAAGAAGAATTTTGGTGATCTTGCTACCATTAAAAGC GAAAGTGAAAAGAAGTTTCTGTGGAAATATATAAACAAGAATGGTGGGCAGTCACCGTATTTTATTGGCC TGTTGATCAGCTTGGATAAGAAGTTCATTTGGATGGACGGAAGCAAAGTCGATTTTGTAGCTTGGGCTAC GGGGGAACCCAACTTTGCAAATGATGATGAAAACTGTGTAACAATGTACACAAATTCAGGGTTCTGGAAT GATATCAACTGCGGTTATCCAAATAACTTCATCTGCCAGCGACATAATAGCAGCATCAATGCCACTGCCA TGCCTACCACACCCCCAACTCCAGGTGGCTGCAAGGAAGGTTGGCATTTGTACAACAACAAGTGCTTTAA AATTTTTGGGTTTGCTGAAGAAGAGAAAAAGACCTGGAAAGAGGCACGGAAAGCTTGCATAGGATTGAAA GGAAACCTGGTGTCCATAGAGAATGCAAAAGAGCAAGCATTTGTCACTTATCACATGAGAGACTCCACTT TCAACGCCTGGACTGGACTGAATGACGTCAACTCAGAGCACACGTTCCTTTGGACAGACGGACGAGGAGT TCATTATACAAACTGGGGGAAAGGCTATCCCGGCGGAAGGAGAAGCAGTCTGTCGTACGAAGATGTTGAC TGTGTTGTTGTGATTGGTGGCAATTCACGAGAGGCAGGGACCTGGATGGATAGCACCTGTGACAGTAAAC AAGGCTATATATGCCAAACACAGACCGACCCTTCTGTGCCTATCTCTCCAACCACGGCACCAAAAGATGG CTTTGTTAAGTATGGTAAAAGCAGCTATGCCCTTATGAAATTGAAGTCGCCATGGCACGAAGCAGAGAAA TACTGCAAGGATCGTACTTCCCTGCTTGCCAGCATTCTTGACCCCTATAGTAATGCATTTGCATGGATGA AAATGCACCCATTTAATGTACCCATATGGATTGCCCTGAACAGCAACTTGACCAACAATGAGTACACTTG GACAGATAAATGGAGGGTGCGGTACACTAACTGGGGAACTGATGAACCCAAGCTTAAGTCAGCATGCGTT TACATGGATGTCGATGGCTACTGGAAGACATCATACTGCAATGAAAGCTTTTACTTTCTCTGCAAAAAAT CAGACGAAATCCCGGCTACGGAACCCCCACAGCTGCCTGGCAAATGTCCAGAGTCAGAGCAAACTGCGTG GATCCCTTTCCACGGCCACTGTTACTACATTGAATCTTCTTTTACAAGGAGTTGGGGTCAAGCCTCCCTG GAGTGCCTTCGAATGGGTGCCTCCCTGGTTTCCATCGAGACTGCTGCCGAATCCAGTTTTCTGTCATACC GTGTTGAACCTCTTAAAAGTAAAACCAATTTTTGGATAGGCATGTTCCGAAATGTTGAAGGAAAGTGGCT TTGGTTGAATGACAATCCTGTGTCCTTTGTCAACTGGAAAACGGGTGACCCTTCGGGTGAACGGAATGAT TGTGTAGTTCTATCTTCATCTTCTGGCCTTTGGAATAATATCCACTGTACTTCATACAAAGGATTTATTT GTAAAATGCCAAAAATTATTGATCCTGTAACTACACATTCATCTATTACAACCAAAGCTGACCAAAGGAA GATGGATCCTCAACCCAAGGGCTCTTCCAAAGCAGCGGGAGTGGTCATCGTGGTCCTTCTGATTGTGATA GGTGCCGGTGTTGCGGCCTATTTCTTTTACAAGAAAAGGCGTGTACTGCACATACCTCAAGAGGCCACCT TTGAAAACACTCTCTACTTCAACAGTAATCCAAGTCCTGGAACAAGTGACACGAAAGATCTCGTGGGCAA CATCGAACAGAATGAGCATGCGGTCATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106123
Insert Size	4371 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106123.2, NP_001099593.2
RefSeq Size	5233 bp
RefSeq ORF	4371 bp
Locus ID	291327

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