Ddx39b (NM_133300) Rat Untagged Clone

CAT#: RN203564

Ddx39b (untagged ORF) - Rat HLA-B associated transcript 1 (Bat1), (10 ug)



  "NM_133300" in other vectors (3)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Bat1; Bat1a; p47
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN203564 representing NM_133300
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGAACGATGTGGACAATGAGCTCTTGGACTATGAAGACGATGAGGTGGAGACGGCCGCTGGGG
CAGATGGGACCGAAGCTCCCGCCAAGAAAGACGTCAAGGGCTCCTATGTCTCCATCCACAGCTCCGGCTT
TCGAGATTTCCTACTTAAGCCAGAGTTGCTCCGGGCCATTGTTGACTGTGGCTTTGAGCATCCATCAGAG
GTCCAGCATGAATGCATCCCCCAGGCCATTCTGGGGATGGATGTCCTGTGCCAGGCCAAGTCAGGCATGG
GAAAAACAGCAGTGTTTGTCCTGGCCACACTGCAGCAGCTGGAGCCAGTTACTGGGCAGGTGTCAGTGCT
GGTGATGTGTCACACCAGGGAGCTGGCTTTCCAGATCAGCAAGGAATATGAGCGCTTCTCAAAGTACATG
CCGAATGTCAAGGTGGCAGTGTTTTTTGGCGGACTGTCTATCAAGAAGGATGAAGAGGTGCTGAAGAAGA
ACTGCCCACACATTGTTGTGGGGACTCCTGGCCGAATTCTAGCCCTGGCCCGAAATAAGAGCCTGAACCT
CAAACACATTAAACACTTTATCTTGGACGAATGTGACAAGATGCTTGAACAGCTCGACATGCGTCGGGAT
GTCCAGGAAATTTTTCGCATGACCCCCCATGAGAAGCAGGTCATGATGTTCAGTGCTACCTTGAGCAAAG
AGATCCGCCCAGTGTGCCGCAAGTTCATGCAAGATCCTATGGAGATCTTCGTGGATGACGAGACCAAGTT
GACGCTGCACGGGTTGCAGCAATACTACGTGAAACTGAAGGACAACGAGAAGAACCGGAAGCTCTTTGAC
CTTCTCGATGTCCTCGAGTTCAACCAGGTTGTGATCTTTGTGAAGTCCGTGCAGCGCTGCATCGCCTTGG
CCCAGCTTCTAGTGGAGCAGAACTTCCCAGCCATTGCTATCCACCGCGGGATGCCCCAGGAGGAGAGGCT
CTCTCGGTATCAGCAGTTCAAGGATTTTCAGCGGAGGATACTTGTGGCTACCAACCTGTTTGGCCGTGGC
ATGGACATTGAGCGCGTGAACATCGCTTTCAACTATGACATGCCAGAGGACTCCGACACCTACCTGCACA
GGGTGGCCAGAGCGGGCCGGTTTGGCACCAAGGGCTTGGCCATCACATTTGTGTCAGACGAGAACGATGC
CAAGATCCTGAATGACGTGCAGGACCGTTTCGAGGTCAACATCAGTGAGCTGCCCGATGAGATCGACATT
TCCTCCTACATTGAACAGACACGGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133300
Insert Size 1287 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_133300.3, NP_579834.2
RefSeq Size 1711 bp
RefSeq ORF 1287 bp
Locus ID 114612
UniProt ID Q63413
Gene Summary Involved in nuclear export of spliced and unspliced mRNA. Assembling component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC and CHTOP onto mRNA. Also associates with pre-mRNA independent of ALYREF/THOC4 and the THO complex. Involved in the nuclear export of intronless mRNA; the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA; its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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