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Ddx39b (NM_133300) Rat Untagged Clone
CAT＃: RN203564
Ddx39b (untagged ORF) - Rat HLA-B associated transcript 1 (Bat1), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Bat1; Bat1a; p47 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN203564 representing NM_133300
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGAACGATGTGGACAATGAGCTCTTGGACTATGAAGACGATGAGGTGGAGACGGCCGCTGGGG CAGATGGGACCGAAGCTCCCGCCAAGAAAGACGTCAAGGGCTCCTATGTCTCCATCCACAGCTCCGGCTT TCGAGATTTCCTACTTAAGCCAGAGTTGCTCCGGGCCATTGTTGACTGTGGCTTTGAGCATCCATCAGAG GTCCAGCATGAATGCATCCCCCAGGCCATTCTGGGGATGGATGTCCTGTGCCAGGCCAAGTCAGGCATGG GAAAAACAGCAGTGTTTGTCCTGGCCACACTGCAGCAGCTGGAGCCAGTTACTGGGCAGGTGTCAGTGCT GGTGATGTGTCACACCAGGGAGCTGGCTTTCCAGATCAGCAAGGAATATGAGCGCTTCTCAAAGTACATG CCGAATGTCAAGGTGGCAGTGTTTTTTGGCGGACTGTCTATCAAGAAGGATGAAGAGGTGCTGAAGAAGA ACTGCCCACACATTGTTGTGGGGACTCCTGGCCGAATTCTAGCCCTGGCCCGAAATAAGAGCCTGAACCT CAAACACATTAAACACTTTATCTTGGACGAATGTGACAAGATGCTTGAACAGCTCGACATGCGTCGGGAT GTCCAGGAAATTTTTCGCATGACCCCCCATGAGAAGCAGGTCATGATGTTCAGTGCTACCTTGAGCAAAG AGATCCGCCCAGTGTGCCGCAAGTTCATGCAAGATCCTATGGAGATCTTCGTGGATGACGAGACCAAGTT GACGCTGCACGGGTTGCAGCAATACTACGTGAAACTGAAGGACAACGAGAAGAACCGGAAGCTCTTTGAC CTTCTCGATGTCCTCGAGTTCAACCAGGTTGTGATCTTTGTGAAGTCCGTGCAGCGCTGCATCGCCTTGG CCCAGCTTCTAGTGGAGCAGAACTTCCCAGCCATTGCTATCCACCGCGGGATGCCCCAGGAGGAGAGGCT CTCTCGGTATCAGCAGTTCAAGGATTTTCAGCGGAGGATACTTGTGGCTACCAACCTGTTTGGCCGTGGC ATGGACATTGAGCGCGTGAACATCGCTTTCAACTATGACATGCCAGAGGACTCCGACACCTACCTGCACA GGGTGGCCAGAGCGGGCCGGTTTGGCACCAAGGGCTTGGCCATCACATTTGTGTCAGACGAGAACGATGC CAAGATCCTGAATGACGTGCAGGACCGTTTCGAGGTCAACATCAGTGAGCTGCCCGATGAGATCGACATT TCCTCCTACATTGAACAGACACGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_133300 |
| Insert Size | 1287 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133300.3, NP_579834.2 |
| RefSeq Size | 1711 bp |
| RefSeq ORF | 1287 bp |
| Locus ID | 114612 |
| UniProt ID | Q63413 |
| Gene Summary | Involved in nuclear export of spliced and unspliced mRNA. Assembling component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC and CHTOP onto mRNA. Also associates with pre-mRNA independent of ALYREF/THOC4 and the THO complex. Involved in the nuclear export of intronless mRNA; the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA; its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR203564 | Ddx39b (Myc-DDK-tagged ORF) - Rat HLA-B associated transcript 1 (Bat1), (10 ug) |
CNY 3,990.00 |
|
| RR203564L3 | Lenti ORF clone of Ddx39b (Myc-DDK-tagged ORF) - Rat HLA-B associated transcript 1 (Bat1), (10 ug) |
CNY 6,080.00 |
|
| RR203564L4 | Lenti ORF clone of Ddx39b (mGFP-tagged ORF) - Rat HLA-B associated transcript 1 (Bat1), (10 ug) |
CNY 6,650.00 |
