Atf6 (NM_001107196) Rat Untagged Clone

CAT#: RN204267

Atf6 (untagged ORF) - Rat activating transcription factor 6 (Atf6), (10 ug)



  "NM_001107196" in other vectors (3)

CNY 8,460.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN204267 representing NM_001107196
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCGCCTTTTAGTCCGGTTCTTCCTCATGGACCAGGTGAAGACTGGGAGTCCACGTTGTTTGCTG
AGCTTGGCTATTTCACAGACACGGATGATGTCCAGTTTGATGCAGCACATGAGACTTACGAAAATAATTT
TGATCATCTTAATTTTGATTTGGATTTGATGCCTTGGGAGTCAGACATATGGAGCCCCAGCAGCCACTTC
TGCTCAGACATTAAGGCAGAGCCCCAGCCTCTTTCTCCGGCTTCCTCCAGTTGTTCTGTCTCTTCTCCTC
GGTCCACAGACTCCTGTTCTTCAACTCAGCACGTTCCTGAGGAGTTGGACTTGTTGTCTAGTTCTCAGTC
TCCCCTTTCCTTGTATGGCGACAGCTGTCACAGCCCCTCCTCAGCAGAGCCACTGAAGGAAGAGAAGCCT
GTCACTGGTCCTGGAAACAAAACGGAACATGGACTGACTCCAAAGAAAAAAATCCAGATGAGTTCAAAAC
CTTCAGTTCAGCCCAAGCCTTTGTTACTTCCAGCAGCACCCAAGACTCCAGCAAACGCCAGTGTCCCAGC
AAAGACCATCATCATTCAGACACTGCCAGCCCTGATGCCACTGGCAAAGCAGCAGTCGATTATCAGTATA
CAGCCTGCGCCCACCAAAGGTCAGACTGTTTTGCTCTCTCAGCCTGCTGTGGTTCAACTTCAGACCCCTG
GAGTTCTGCCCTCTGCTCAGCCAGTACTTGCTGTCACTGGCGGAGCCACACAGCTACCTAACCATGTGGT
GAATGTGGTGCCAGCCCCGGTGGTGAACAGCCCGGTGAACGGAAAACTGTGTGTGACTAAACCTGTTCTA
CAAAGCAGCACCAGAAGCACGGGTTCAGATATTGCCGTACTGAGGAGACAGCAGCGTATGATCAAGAACC
GAGAGTCTGCTTGTCAGTCACGAAAGAAGAAGAAAGAGTATATGCTAGGCCTGGAGGCCAGGTTGAAGGC
CGCCCTCTCCGAGAATGAGCAGCTGAAGAAGGAGAATGGGTCCCTGAAGCGACAGCTGGACCAGGTGGTG
TCAGAGAACCAGAGGCTCAAAGTCCCAAGTCCAAAGCGCAGAGCTGTCTGTGTGATGATAGTATTAGCAT
TTATCATGCTGAACTATGGGCCCATGAGCATGCTGGAGCAAGATTCCAGGAGAGTGAAACCTAGTGTGAG
CCCTGCCAATCAAAGGAGGCATCTTTTAGAGTTTTCAGCAAAAGAAGTTAAAGACACGTCAGATGGTGAC
AACCAGAAAAACAGTTACAGATATGATCATTCTGTGTCCAATGACAAAGCCTTAATGGTGCTAAGTGAAG
AACCATTACTTTATATCCCTCCACCTCCATGTCAGCCCCTCATTAACACGACAGAGTCTCTCAGGTTGAA
CCATGAACTTCGAGGCTGGGTTCATAGACATGAAGTGGAAAGGACCAAATCGAGAAGAATGACAAATAGC
CAGCAGAAAACCCGCATTCTCCAGGGTGCTCTGGAACAGGGCTCGAATTCTCAGCTGATGGCTGTCCAGT
ACACAGAAACCACTAGCATCAGTAGGAATTCTGGGAGTGAGCTGCAGGTGTATTACGCTTCGCCTGGAAG
TTACCAAGGCTTCTTTGACGCCATCCGCAGGAGGGGAGATACATTTTACGTCGTATCATTTCGAAGGGAT
CACCTGCTATTACCAGCTACCACCCATAACAAGACCACAAGACCGAAGATGTCCATTGTGTTACCAGCAA
TAAACATAAATGATAACGTGATCAATGGGCAGGATTATGAAGTAATGATGCAGATTGACTGTCAGGTCAT
GGACACCAGGATCCTCCACATCAAAAGCTCCTCGGTTCCCCCGTATCTCCGAGATCATCAGAGAAACCAA
ACCAGCACCTTCTTCGGTTCCCCTCCCACAGCCACAGAGACGACCCATGTGGTCAGCACCCTCCCTGAGT
CGGTACAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001107196
Insert Size 1971 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001107196.1, NP_001100666.1
RefSeq Size 3217 bp
RefSeq ORF 1971 bp
Locus ID 304962
UniProt ID G3V909
Gene Summary Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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