Wnk1 (NM_001002823) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hsn2; Prkwnk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204274 representing NM_001002823 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGGCACCGCAGAGAAGCAGAGCGGCACTCCTGGCTTCCTTTCGCCTCCGGCTCCAGTCCCCA AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCCGCGGTCGCCGACTCCGGAATTGG CAGGACAGAGGAATACCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACT ACCCCCACGGAGCACCGATTTTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACGGCGTTGGAGCTGC CGGGCCTCCCTCTCTCTATCCCTCAACCTAGTGTACCCGCAGTGGTGCCTCAGAGTGCTCCACCCGAGCC TCACAGAGAGGAAACTTTGACGGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCGCGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG TGTCCCAGCCCAGCCTCGTGGGTAGCAAAGAGGAGCCGCCGCCGTCCAGAAGTGGAAGTGGCAGCGGTGG TGCCAGTGCCAAAGAGCCGCAGGAGGAAAGGAACCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA CGGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAAGCTGAAATGCTAAAGGGTCTTCAACATCCTAACATT GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA TGACATCTGGAACACTTAAAACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG GTGTCGGCAAATCCTTAAAGGACTTCAATTTCTCCATACACGAACTCCCCCGATTATTCACCGGGATCTT AAATGTGACAACATCTTTATCACTGGTCCTACCGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA GAAGTACGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACGTCTGAATAT CCATACTCAGAGTGCCAAAATGCTGCTCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT TTGACAAAGTAGCAATTCCTGAAGTGAAGGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA GAAGAAGATGATGGAGAAAAGATAGCTATCAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGG GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC TCAAGAAATGGTCGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT AGGGTATCATTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAACAAGAAAAAAGAAAAC AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGTGTTTCCCAGGCAGGAATCCAGCCACT CTCTGTTGCTAGCACTGGCATACCCACTGCTCCCACCACATCCGCTTCAGTTTCTACTCAAGTAGAGCCT GAAGAACCCGAGGCAGATCAGCATCAACAGCTGCAGTATCAGCAACCTAGTATATCTGTGTTGTCTGATG GAACAGTTGACAGTGGTCAAGGATCATCTGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC CTATGGCTCCCAACATGAGCAGGCACATTCTATTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGCCTCAGTCCGTGGCGCATCCGTGTGGGG GGACCCCAACATACCCAGAATCACAGATATTTTTCCCAACTATTCATGAACGTCCAGTTTCTTTTTCACC ACCTCCTACCTGTCCACCGAAAGTAGCCATTTCCCAGCGACGTAAGAGTACCTCCTTCCTGGAAGCTCAA ACTCGCCACTTCCAACCCCTGCTGAGGACTGTTGGCCAAAATCATCTTCCACCTGGTGGCAGCCCAACTA ACTGGACACCAGAGGCCATAGTTATGTTGGGTACTACAGCCAATAGAGTAAATAGAGAGCTATGTGAGAT GCAGGTCCAACCTGTGTTTGAGACAACCCAGATTTACAGTGACTATAGACCTGGACTAGTACTGGCAGAA GAAGCTCACTATTTTATTCCTCAGGAAACAGTATATCTAGCTGGGGTGCATTACCAGGCCCATGCGGCAG GACAGTATGAGGGTATTTCATACAACTCACCAGTACTGTCAAGTCCTATGAAACAGATAACTGAACAGAA GCCAGTGCCTGGGTGCCCTGCCTCAAGTTCTGTCTTTGAATTTCCTTCTGGACAGGCTTTCCTAGTAGGA CACCTTCAGAATTTAAGATTAGATTCTGGACCAAGTCCAGCATCACCCCTGTCTAGTATTTCTGCGCCTA ACAGTACAGATGCTACACATTTGAAATTTCACCCTGTCTTTGTACCTCATTCTGCACCAGCTGTGTTAAC TCATAGCAATGAGAACAGAAGCAACTGTGTATTTGAATTTCATGCTCAAACACCAAGTTCCTCTTCAGGA GAAGGAGGTGGGATTTTACCTCAACGTGTTTACCGAAATCGACAGGTTGCAGTGGACTCAAGTCAGGAAG AACTGTCTCCTCAGTCAGTTGGATTACACTGCCACCTGCAGCCTGTTACTGAAGAACAGCGTAATAACCA TACCCCAGAATTGACCATTTCTGTGGTAGAACCTATGGGACAAAACTGGCCAGTAGGAAGCCCAGAATAT TCCAGTGATTCCTCTCAAATTACTTCTTCAGATATCAGTGATTTTCAATCACCTCCCCCTACAGGGGGAA CAGCTGCACCTTTTGGCTCTGACGTCTCATTGCCCTATATTCGCCTGCCTCAGACAGTGTTACAAGAATC CCCACTATTCTTCTGTTTCCCCCAAGGAACCACATCTCAGCAGGTCTTATCTGCTTCATATTCTTCAGGA GGATCTGCACTCCATCCACAGGCACAGGGGCAGAACCAGGGCCAGCCATCAAGTAGTTTAGCAGGGGTTC TATCTTCACAACCCGTCCAACATCCTCAGCAGCAGGGAATACAGCCGACTGTACCTCCTCAACAGGCAGT ACAGTATTCACTTCCACAGGCAGCGTCTTCCAGTGAAGGCACTGTCCAGCCAGTGAGTCAGCCTCAAGTG TCAGCTGGAACACAGGGCTTCCCATCTCGACTGCCACCACAATACCCAGGAGATTCAAATATTGCTCCCT CTTCCAACGTGGCTTCTGTTTGCATCCATTCTACAGTCCTAGCCCCTCCTCCTATGCCAACAGAAGCATT GGCCACCCAAGGTTACTTTCCTACAGTAGTGCAGCCTTATGTGGAATCAACTCCTTTGGTTCCTATGGGC AGTGTAGGAGGCCAGGTTCAAGTGTCCCAACCAGCAGTGAGTTTATCGCAACAACCCCCCACTACATCCT CCCAGCAAGCAGTTTTGGAGAGTAGTACACAGGGAGTCTCTCAGGCTGCTCCTCCAGAACAAACTCCAAT AACACAGTCACAACCTACTCAACCTGTTCCATTGGTTTCCTCTGTGGACAGTGCCCATTCAGATGTTGCT TCTGGTATGAGTGATGGCAATGAGAATGCTCCTTCATCCAGCGGGAGGCATGAAGGGAGGACGACAAAAC GTCATTATCGAAAATCTGTAAGAAGTCGTTCTCGTCATGAAAAGACTTCTCGCCCGAAACTGAGGATTTT AAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTGGAGACTCATAATCGGAAAATGGTTACA TTTAAATTTGACCTAGATGGTGACAACCCTGAAGAGATAGCAACAATTATGGTGAACAATGACTTTATTC TAGCCATAGAGAGAGAGTCTTTTGTGGCTCAAGTGCGGGAAATTATTGAAAAGGCTGATGAAATGTTAAG CGAGGATGTCAGTGTGGAGCCTGAGGGTGACCAGGGATTGGAGAGTCTACAAGGAAAGGATGACTATGGC TTTCCAGGTTCTCAGAAATTAGAAGGAGAGTTCAAGCAACCAATTGCTGTATCTTCCATGCCACAACAAA TAGGTGTTCCTACCAGTTCTTTAACACAAGTTGTTCATTCTGCTGGAAGACGATTTATAGTGAGTCCAGT TCCAGAAAGTCGATTACGAGAATCAAAAATTTTCACCAGTGAAATACCAGATCCAGTTGCGGCCTCTACA TCTCAGGGCCCTGGAATGAACTTATCTCACTCCGCTTCATCCCTTAGTCTCCAGCAGGCTTTTTCTGAAC TGAAACATGGTCAGATGACTGAAGGACCCAATACAGCACCTCCAAATTTTAATCACCCAGGACCAACATT TTCTCCCTTTTTGACTAGCATTGCTGGTGTCCAAACTGTAGCAGCATCCACACCATCAGTATCAGTCCCT ATAACAAGCAGCCCTCTTAATGACATTTCCACATCAGTGATGCAGTCAGAGGGTGCACTGCCCACTGACA AAGGCATTGGGGGAGTTACCACCAGCACAGGTGTGGTAGCTTCAGGTGGTCTCACCACATTGTCTGTGTC TGAGACACCAACACTTTCCAGTGCAGTTTCAAGTTCTACAGCGCCTGCAGTTGTCACAGTGTCTACAACA TCCCAACCAGTTCAGGCTTCCACATCTGGGAGCATTGCTTCTAGTACAGGCAGTTTTCCTTCTGGAACAT TTTCCACAACTACAGGTACCACAGTGAGTAGTGTAGCTGTCCCAAATGCTAAGCCTCCAACTGTATTACT GCAACAGGTAGCAGGTAATACTGCTGGGGTAGCCATAGTGACATCAGTTTCTACTACCACTCCATTCCCA GCCATGGCTTCACAGCCATCCCTTCCACTTGGTAGCAGCACATCTGCTCCTACACTAGCTGAAACAGTGG TGGTTAGTGCACACTCACTAGACAAGGCATCTCACAGTAGTACAGCAGGATTGGGTTTGTCCTTTTGCGC ACCGTCCTCTTCTTCCTCCTCTGGAACAGCAGTGTCTAGTTCTGTTTCTCAGCCTGGTATTGTGCATCCT TTGGTCATCTCATCAGCTATAGCTTCTACACCTGTCCTCCCACAACCAGCAGTACCTACTTCTACACCTT TATTACCCCAAGTACCTAATATCCCACCCTTGGTACAACCTGTTGCTAATGTGCCTGCTGTACAGCAAAC ACTCATTCATAGTCAGCCTCAACCAGCTCTACTTCCCAACCAGCCTCACACACACTGTCCCGAAATGGAT GCTGATACCCAGTCCAAAGCTCCTGGGATCGATGATATAAAGACTCTGGAGGAAAAGCTGAGATCGCTCT TTAGTGAGCATAGCTCATCTGGAACTCAGCATGCTTCTGTCTCCCTGGAGACACCCTTAGTAGTAGAGAC TGTCACACCGGGCATCCCTACCACTGCTGTAGCACCAAGTAAACTCATGACTTCCACTACAAGCACTTGC TTGCCACCAACCAATTTACCCTTAGGAACAGCTGGCATGCCAGTTATGCCAGTGGGCACACCTGGTCAAG TTTCTACCCCAGGCACACATGCCTCAGCCCCAGCCAGCACCGCAACAGGAGCAAAACCTGGAACAACTCC CCCAAAGCCATCTTTAACTAAGACTGTGGTGCCGCCAGTGGGTACTGAGCTTTCGGCTGGTACTGTACCC TGTGAGCAGCTGCCACCTTTTCCCGGACCGTCTCTAATTCAGACCCAGCAACCCCTAGAAGATCTTGATG CTCAGTTGAGAAGAACACTTAGTCCAGAGACTATTCCAGTGACACCCGCAGTTGGTCCTTTGTCTACGAT GTCTTCAACGGCAGTTACAGAAGCAGGAAGTCAACCTCAGAAGGATGGCACAGAAGTTCATGTCACAGCA AGTAGCTCAGGAGCTGGTGTTGTTAAGATGGGGAGATTTCAGGTTTCAGTTACAATGGATGATGCCCAGA AAGAGCGTAAAAATAGGTCAGAAGATACAAAGTCGGTTCATTTTGAATCTAGTACATCAGAGTCATCAGT TCTTTCAAGTAGTAGTCCAGAGAGTACCCTGGTAAAGCCAGAGCCTAATGGAATTACTGTTTCTGGCATC TCCTTAGATGTGCCAGACAGTACCCATAGAACACCGACCCCAGAAGCAAAGTCAGAAACAGGACAGCCTA CCAAGGTTGGACGTTTTCAGGTGACAACTACAGCCAACAAAGTGGGTCGTTTCTCTGTTTCAAGAACTGA GGACAAGGTCACAGAGCTCAAAAAAGAGGGACCAGTGACCTCTCCTTTCAGAGATTCAGAACAAACTGTT ATTCCTGCTGCGATACCAAAGAAGGAGAAGCCTGAGCTGGCAGAACCTTCCCATCTGAATGGGCCATCTT CAGACTTGGAGGCTGCCTTTCTAAGTAGGGGTGGAGAGGATGGATCAGGTAGTCCTCACTCTCCCCCTCA CCTGTGTTCAAAGAGCCTTCCCATCCAGACTCTAAGTCAAAGCCTTAGTAATTCATTCAACTCCTCTTAC ATGAGTAGTGACAATGAGTCCGACATTGAAGATGAAGACTTAAGGTTAGAACTGCGACGACTACGGGAAA AACATCTTAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACCAAACT GGGCAAGGTTCCCCCTGCTGTCATTATTCCCCCAGCTGCTCCTCTGTCAGGGAGAAGAAGGAGGCCTACT AAAAGCAAAGGCAGCAAGTCCAGTCGCAGCAGCTCATTGGGCAACAAAAGCCCTCAGCTTTCAGGTAACC TGTCTGGTCAGAGTGGAACTTCAGTCTTAAACCCCCAACAGACCCTCCATCCCCCAGGCAATACCCCAGA GACTGGACACAACCAGCTGTTACAGCCTCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTT ACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGGACCAGCAGCACAAACACCG TTGGAGGAACAGTGAGTAGCCAGGCTGCCCAGGCTCAGCCTCCTGCCATGACTTCCAGTAGGAAGGGCAC ATTCACAGATGACCTGCACAAGTTGGTAGACAACTGGGCCCGAGATGCCATGAATCTTTCAGGCAGGAGA GGCAGCAAAGGACACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCTCCTGGGCAGCTGT GCATTTCCATGACCTCAAACATGGGTGGCTCTACCCCCATCTCTGCAGCATCTGCTACCTCTCTAGGTCA CTTCACCAAGTCCATGTGCCCCCCACAGCAGTATGGTTTTCCAGCTGCCCCATTCGGCACTCAGTGGAGT GGAACAGGTGGTCCAGCACCACAGCCACTTGGTCAGTTCCAACCTGTAGGAACCACATCCTTGCAAAATT TCAACATCAGCAATTTGCAGAAGTCCATCAGCAACCCCCCAGGTTCCAACCTACGGACCACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001002823
Insert Size	7905 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001002823.2, NP_001002823.2
RefSeq Size	10497 bp
RefSeq ORF	7905 bp
Locus ID	116477
UniProt ID	Q9JIH7
Gene Summary	catalyzes the phosphorylation of myelin basic protein in vitro; has autophosphorylation activity; may regulate the thiazide-sensitive Na-Cl cotransporter NCC [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This variant represents the exon combination of the dorsal root ganglia and sciatic nerve variant inferred from the mouse sequence described in Figure 2F of PubMed ID 18521183. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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