Cpeb1 (NM_001106276) Rat Untagged Clone

CAT#: RN204288

Cpeb1 (untagged ORF) - Rat cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10 ug)



  "NM_001106276" in other vectors (3)

CNY 7,130.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN204288 representing NM_001106276
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTTTTCTCTGGAAGAAGAATCAGGAAGGATAAAGGACTGCTGGGACAACCAAGAGGTTCCTGCAC
TCTCTACATGTAGCAATGCCAATATCTTTCGAAGGATAAATGCCATTTTGGATGATTCACTGGACTTCAG
TAAAGTCTGCACAACACCTATAAATCGAGGAATTCATGATCAGTTGCCAGACTTCCAGGACTCTGAAGAA
GCAATTACAAGCAGGATGCTTTTCCCCACCTCTGCGCAAGAATCTCCCCGCGGCCTCCCAGATGCAAACG
GTTTGTGCCTTGGCCTGCAGTCACTCAGTCTGACTGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGA
CTCCTCAGCCCAGAGCAACACACAGTCAGTATTAAGCATGCTCCAAAATCCACTGGGAAATGTCTTAGGA
AAAACCCCCTTGAGCTTCCTGTCTCTGGATCCCCTTGGGTCTGACTTGGACAAGTTCCCAGCGCCCTCAG
TTAGAGGATCTCGCCTGGACACCCGGCCTATCCTGGACTCCCGTTCTAGCAGCCCCTCTGACTCAGACAC
GAGTGGCTTCAGCTCTGGATCAGATCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCCCCTCCTCTG
CCCTTTCTTTCTATGACGGGAAATGGTCCCAGAGACCCTCTAAAGATGGGAGTTGGGTCTCGAATGGACC
AAGAGCAAGCTGCTCTTGCTGCAGTTGCTCCCTCTCCAACCAGTGCTCCAAAGAGATGGCCAGGAACTTC
TGTGTGGCCATCTTGGGACCTTCTCGGAGCTCCTAAAGACCCATTCAGCATAGAGAGAGAGGCTAGACTC
CACAGGCAGGCCGCAGCTGTGAATGAAGCTACATGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACTACA
AGAACCCCATCTACTCATGCAAGGTGTTCCTAGGAGGTGTTCCTTGGGACATTACAGAAGCTGGATTGGT
TAACACCTTCCGTGTTTTTGGCTCTCTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCACCCCCGGTGT
CCTCCCAAAGGTAATATGCCTAAAGGGTATGTGTATTTGGTGTTTGAACTAGAGAAGTCCGTCCGGGCCT
TGCTTCAGGCTTGTTCTCATGACCCACTGAGCCCAGATGGCCTAAGTGAATATTATTTCAAGATGTCCAG
CCGAAGGATGCGCTGCAAAGAAGTACAGGTAATCCCTTGGGTCTTGGCTGACAGCAACTTTGTCTGGAGC
CCATCTCAGAGGCTGGATCCCAGCAGGACGGTGTTTGTTGGTGCCTTGCACGGGATGCTCAATGCTGAGG
CTCTGGCTGCCATCTTGAATGACCTATTTGGTGGAGTGGTGTATGCTGGGATTGACACAGATAAGCACAA
GTACCCCATTGGGTCTGGTCGTGTGACTTTCAATAACCAACGTAGTTACCTGAAAGCAGTCACTGCTGCT
TTTGTGGAGATCAAAACCACCAAGTTTACCAAGAAGGTTCAGATCGACCCCTATCTAGAAGATTCTCTGT
GTCTTATCTGCAGTTCACAACCTGGTCCTTTCTTCTGCCGAGATCAGGTCTGCTTCAAGTACTTTTGCCG
AAGCTGCTGGCACTGGCGACACAGCATGGAAGGCCTACGCCACCACAGCCCGCTAATGAGGAACCAGAAG
AACTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001106276
Insert Size 1686 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001106276.1, NP_001099746.1
RefSeq Size 2615 bp
RefSeq ORF 1686 bp
Locus ID 293056
UniProt ID P0C279
Gene Summary Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the 3' UTR of mRNAs. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation. Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses. Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation. Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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