Cpeb1 (NM_001106276) Rat Untagged Clone
CAT#: RN204288
Cpeb1 (untagged ORF) - Rat cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10 ug)
CNY 7,130.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN204288 representing NM_001106276
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTTTCTCTGGAAGAAGAATCAGGAAGGATAAAGGACTGCTGGGACAACCAAGAGGTTCCTGCAC TCTCTACATGTAGCAATGCCAATATCTTTCGAAGGATAAATGCCATTTTGGATGATTCACTGGACTTCAG TAAAGTCTGCACAACACCTATAAATCGAGGAATTCATGATCAGTTGCCAGACTTCCAGGACTCTGAAGAA GCAATTACAAGCAGGATGCTTTTCCCCACCTCTGCGCAAGAATCTCCCCGCGGCCTCCCAGATGCAAACG GTTTGTGCCTTGGCCTGCAGTCACTCAGTCTGACTGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGA CTCCTCAGCCCAGAGCAACACACAGTCAGTATTAAGCATGCTCCAAAATCCACTGGGAAATGTCTTAGGA AAAACCCCCTTGAGCTTCCTGTCTCTGGATCCCCTTGGGTCTGACTTGGACAAGTTCCCAGCGCCCTCAG TTAGAGGATCTCGCCTGGACACCCGGCCTATCCTGGACTCCCGTTCTAGCAGCCCCTCTGACTCAGACAC GAGTGGCTTCAGCTCTGGATCAGATCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCCCCTCCTCTG CCCTTTCTTTCTATGACGGGAAATGGTCCCAGAGACCCTCTAAAGATGGGAGTTGGGTCTCGAATGGACC AAGAGCAAGCTGCTCTTGCTGCAGTTGCTCCCTCTCCAACCAGTGCTCCAAAGAGATGGCCAGGAACTTC TGTGTGGCCATCTTGGGACCTTCTCGGAGCTCCTAAAGACCCATTCAGCATAGAGAGAGAGGCTAGACTC CACAGGCAGGCCGCAGCTGTGAATGAAGCTACATGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACTACA AGAACCCCATCTACTCATGCAAGGTGTTCCTAGGAGGTGTTCCTTGGGACATTACAGAAGCTGGATTGGT TAACACCTTCCGTGTTTTTGGCTCTCTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCACCCCCGGTGT CCTCCCAAAGGTAATATGCCTAAAGGGTATGTGTATTTGGTGTTTGAACTAGAGAAGTCCGTCCGGGCCT TGCTTCAGGCTTGTTCTCATGACCCACTGAGCCCAGATGGCCTAAGTGAATATTATTTCAAGATGTCCAG CCGAAGGATGCGCTGCAAAGAAGTACAGGTAATCCCTTGGGTCTTGGCTGACAGCAACTTTGTCTGGAGC CCATCTCAGAGGCTGGATCCCAGCAGGACGGTGTTTGTTGGTGCCTTGCACGGGATGCTCAATGCTGAGG CTCTGGCTGCCATCTTGAATGACCTATTTGGTGGAGTGGTGTATGCTGGGATTGACACAGATAAGCACAA GTACCCCATTGGGTCTGGTCGTGTGACTTTCAATAACCAACGTAGTTACCTGAAAGCAGTCACTGCTGCT TTTGTGGAGATCAAAACCACCAAGTTTACCAAGAAGGTTCAGATCGACCCCTATCTAGAAGATTCTCTGT GTCTTATCTGCAGTTCACAACCTGGTCCTTTCTTCTGCCGAGATCAGGTCTGCTTCAAGTACTTTTGCCG AAGCTGCTGGCACTGGCGACACAGCATGGAAGGCCTACGCCACCACAGCCCGCTAATGAGGAACCAGAAG AACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001106276 |
Insert Size | 1686 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001106276.1, NP_001099746.1 |
RefSeq Size | 2615 bp |
RefSeq ORF | 1686 bp |
Locus ID | 293056 |
UniProt ID | P0C279 |
Gene Summary | Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the 3' UTR of mRNAs. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation. Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses. Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation. Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR204288 | Cpeb1 (Myc-DDK-tagged ORF) - Rat cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10 ug) |
CNY 7,130.00 |
|
RR204288L3 | Lenti ORF clone of Cpeb1 (Myc-DDK-tagged ORF) - Rat cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10 ug) |
CNY 9,120.00 |
|
RR204288L4 | Lenti ORF clone of Cpeb1 (mGFP-tagged ORF) - Rat cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10 ug) |
CNY 9,980.00 |