Phka1 (NM_022626) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Pcyt1b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204493 representing NM_022626 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGCCGGAGTAACTCGGGGGTCCGCCTGGACGGCTATGCTCGGCTGGTGCATCAGACCATCCTGT GCCATCAGAATCCAGTGACAGGCTTGCTTCCGGCCAGCTATGATGAGAAAGATGCCTGGGTCCGAGATAA TGTGTATAGCATCCTGGCTGTGTGGGGTTTAGGCCTGGCATATCGAAAGAATGCAGACCGTGATGAGGAC AAGGCAAAGGCCTATGAACTGGAGCAGAGTGTAGTGAAGTTAATGAGGGGACTGCTGCACTGCATGATCA GACAGGTGGATAAAGTAGAGTCTTTCAAGTACAGTCAGAGTACTAAAGATAGCCTTCACGCCAAGTACAA CACCAAAACTTGTGCCACGGTAGTGGGTGATGACCAGTGGGGACACCTGCAGTTGGATGCTACTTCTGTG TACCTGCTCTTCCTAGCACAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGACGAAGTGAATT TTATACAGAACCTTGTGTTTTACATTGAAGCCGCATATAAAACTGCTGACTTTGGGATATGGGAACGCGG AGATAAGACAAACCAAGGCATCTCAGAATTGAATGCAAGTTCAGTTGGAATGGCCAAGGCAGCCCTGGAA GCACTAGATGAATTAGACTTGTTTGGTGTGAAAGGTGGGCCGCAATCAGTTATCCATGTCCTGGCTGACG AAGTACAACACTGCCAGTCGATCCTGAATTCACTACTGCCCAGGGCTTCAACATCGAAAGAAGTTGATGC CAGTCTGCTGTCAGTGGTCTCTTTCCCAGCCTTTGCTGTAGAGGACAGTCATTTGGTGGAGATCACCAAA CAGGAGATCATCACCAAACTTCAGGGTCGTTATGGTTGCTGTCGTTTTCTGCGTGATGGATATAAAACTC CTAAAGAGGATCCTAATCGCCTATACTATGAACCAGCTGAGCTGAAGCTATTTGAAAACATAGAGTGTGA ATGGCCACTGTTCTGGACATACTTTATCCTTGATGGGATCTTCAGTGGCAACACAGAACAGGTTCAAGAA TATAGAGAGGCTCTTGATGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCACTACTTCCAGAGCTGTACA GTGTTCCTCCTGACAGGGTTGATGAAGAATATCAAAATCCTCACACCGTGGATCGAGTCCCTATGGGAAA ATTGCCTCACATGTGGGGTCAGTCCCTATATATTTTAGGAAACTTGATGGCGGAGGGATTTTTAGCTCCT GGAGAAATTGATCCCCTGAATCGTAGGTTTTCCACTGTCCCAAAGCCAGACGTAGTGGTTCAAGTCTCCA TTCTGGCTGAAACAGAAGAAATCAAGGCCATTTTGAAGGACAAAGGAATTGATGTGGAGACCATTGCTGA AGTGTACCCCATAAGAGTACAGCCAGCTCGTATTCTCAGCCACATTTATTCCAGTCTAGGATGCAACAGT AGAATGAAACTCAGTGGACGACCCTACAGGCTCATGGGTGTACTTGGAACGTCAAAACTTTATGACATTC GCAAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAGCAGTTCTACTTGGCTCTGGACAACCA GATGATAGTCGAAATGCTCCGGACAGATCTCTCCTACCTCTGCAGCCGGTGGAGGATGACAGGCCAGCCC ACCATCACTTTCCCCATCTCACACACCATGCTGGATGAAGATGGAGCCAGCTTGAATTCAAGTATCCTGG CAGCACTCCGGAAAATGCAGGATGGTTATTTTGGTGGGGCCAGGATCCAAACAGGTAAGCTGTCAGAGTT TTTGACAACATCTTGCTGCACACACTTAAGCTTCATGGACCCTGGACCCGAGGGTAAGCTGTACAGCGAA GATTATGATGAAGACTATGATGATGAGCTGGACTCTGGCAACTGGATGGATAGCTATGACTCAACACGTA ATGCTCGCTGTGGTGATGAAGTTGCCCGTTATTTAGACCACCTTTTGGCACACACTGGTCCCCATCCTAA ACTAACTCCCACCTCACGGAAGGGAGGGCTAGATCGGTTCCGAGCTGCTGTGCAAACAACTTGCGACTTA ATGTCCTTGGTGGCCAAGGCCAAGGAGCTGCATATACAGAATGTTCACATGTATCTTCCTACAAAGTTAT TTCAGCCTTCTCGCCCTTCACTCAACTTACTTGACTCACCTGAATCTCCACAAGATAGCCAGGTTCCTTC TGTTCGTGTAGAAGTCCATCTTCCTAGGGACCAGTCTGGGGAAGTGGACTTCCAGTCATTGGTTTCACAG TTGAAGGAGACCTCAAGCTTACAGGAGCAAGCTGATATACTCTACATGCTATATACAATGAAAGGACCTG ACTGGAACACTGAATTGTACGAAGAAGGAGGCAGTACTGTGAGAGAGCTTCTTAGTGAACTATATGTCAA AGTCGGTGAAATTCGGCACTGGGGTCTGATCCGATACATCTCTGGGATCTTACGGAAGAAGGTGGAGGCA CTTGATGAGGCCTGCACAGACCTTCTGTCCTACCAGAAACACCTGACAGTAGGGCTTCCTCCAGAACCTC GAGAGAAGACCATCTCTGCGCCTCTACCGTACGAGGCACTCACTAAGCTGATAGATGAAGCCAGCGAAGG AGACATGAACATCTCCACCCTGACACAGGAAATAATGGTCTATCTTGCCATGTATATGAGAACTCAGCCT GGCCTTTTTGCAGAAATGTTCCGACTTCGAATTGGTTTGATCATACAAGTTATGGCAACAGAACTGGCCC ACTCTCTGCGATGTTCAGCTGAAGAAGCCACAGAGGGCCTGATGAATCTCAGTCCTTCAGCCATGAAGAA TCTCCTGCATCACATCCTCAGCGGCAAAGAGTTTGGAGTGGAACGAAGCGTTCGTCCCACTGATTCAAAT GTGAGTCCTGCTATTTCCATCCATGAGATCGGTGCTGTCGGAGCAACAAAAACAGAACGAACTGGAATCA TGCAGTTAAAAAGTGAGATCAAGCAGGTGGAATTCCGTAGGCTGTCTGTCTCACCCGAGAGTCAGACTAG TGGTGGTCATCCCTCAAGTATAGATTTGATGTCACCGACCTTTCTGTCCCCTGCAGCCTGTATTTCTGCG AGCAGTGGGTCCTTTCCTACTGTATGTGAGCCACAGACATCTAAAGATAGTCGTCAAGGCCAATGGCAAC GCAGGAGAAGACTAGATGGAGCACTAAATAGAGTACCAATTGGATTTTATCAAAAAGTGTGGAAAATTTT GCAGAAATGTCACGGGCTTTCTGTGGAAGGTTTTGTTCTTCCCTCTTCAAGCACTAGGGAGATGACCCCA GGCGAGATTAAATTCTCTGTCCACGTGGAGTCCGTCCTGAACCGTGTGCCTCAGCCAGAGTACCGTCAGC TTCTGGTTGAAGCCATCCTCGTTCTCACCATGCTGGCAGATATTGAAATCCATAGCATTGGGAGCATCAT TGCTGTGGAAAAAATAGTTCATATTGCCAATGACTTGTTCCTTCAGGAACAGAAAACCCTCGGCGCAGAT GATATCATGTTGGCAAAGGATCCTGCATCTGGCATCTGTACTCTTCTGTATGACAGTGCACCCAGTGGCA GATTTGGCACCATGACCTACCTCTCCAAGGCAGCTGCCACCTACGTGCAGGAGTTTCTGCCACACAGCCT CTGTGCCATGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022626
Insert Size	3726 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022626.2, NP_072148.2
RefSeq Size	4359 bp
RefSeq ORF	3726 bp
Locus ID	64561
Gene Summary	catalyzes phosphatidylcholine (PC) synthesis; mediates maintenance of cell membrane PC homeostasis [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.