Ssc5d (NM_001134545) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1565772
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204574 representing NM_001134545 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGTCTTGGCGTGTCTTCTTGCAATGCTGGTGGGGATCCAGGCTATAGAGCGACTCCGCCTGGCTG ATGGCCCTCATGGCTGTGCAGGACGCCTGGAGGTCTGGTACAGCGGGCGCTGGGGCACCGTGTGTGATGA CGGATGGGACCTTCGGGATGCTGCAGTGGCCTGCCGTGTGTTGGGCTGCGGAGGGGCGCTAGCCGCCCCC GGGGGTGCCTTCTTTGGGGAGGGCACTGGGCCCGTGTGGCTCAGTGAGCTGGCCTGTCGGGGCAGTGAGG GACAGCTGGGTATCTGTCCCCACCGGGGCTGGAAGGCTCATATCTGCTCTCACGAGGAGGACGCGGGCGT CGTCTGTGTAGGTCAGCGTGCAGCTAACTCTAGAGAAGACTCAGTGTCCCTGCTGGATGGGGACCCATGG CTGGCACTGTCTGGGGAGCTGAGCCCCAGCTCAGAGGAGCCCCCTATAACTCATGCTCCCCAGCCGGCAG CAAGCTCCCAGAATGGCCCCCGAAAGAAGAACCCCCGGCCACCCAAGCAGGTCAAGTCCACCCGAGCCCC TGTACTGACAAATGGAGCTCCCCGCCAAGAGCGGCTGCGTCTGGTCTCAGGTCCCCATGGATGTGCCGGC CGCCTGGAAGTCTGGCACGGTGGACGTTGGGGTACTGTCTGCGATGATGGATGGGACCTCCGAGACGCAG CAGTGGCCTGTAGGGAGCTAGGATGTGGGGGAGCGCTGGCCGCCCCTGGAGGTGCCAGATTCGGACCTGG TGAGGGACCCGTGTGGATGGACGATGTCGGGTGTGGAGGAGGAGAGGAGGCCCTCCGGGACTGTCCCCGA AGCCCCTGGGGCCGGAGCAACTGTGACCACACAGAGGATGCAGGGCTGGTCTGTACTGGTCCTGCACCCA GGTTGCGCCTTGCTGATGGTCCCCACGGCTGTGCTGGGCGCCTGGAGGTTTGGCATGGTGGCCGCTGGGG GTCGGTATGTGACGATGCTTGGGATCTTCGTGATGCCGCTGTGGCCTGCAGGGAGCTGGGCTGTGGGGGT GCCCTGGCTGCCCCAGGGGGTGCCTTCTTTGGTGAGGGGGCTGGACCCATCATCCTGGATGACCTCAGAT GTCGAGGAAACGAGACAGCCTTACGATTCTGCCCTGCGAGGCCCTGGGGTCAGCATGACTGTCACCATCG GGAGGATGCTGGGGCTGTGTGTGATGGCATGCCTCTCGGTGGCGATGTGCAGCCTACAGCTCCTGCAGTG GACAGCAACAGAACAACACCCAGGCTCCAGTCTACCTCAGTGGGTCATACGCCAGGCCCAGCAGGCAGTT GGCCTCCTCCTGCTTCTCCTACTGCCCCTCCAGAGCCTGGACCTGAAGCTGGGTCCCCCCAGTTGCGCCT GGTGGCTGGACCCAGCAGGTGCTCTGGTCGGCTGGAGGTATGGCATGATGGACGCTGGGGGACAGTATGT GATGACAGCTGGGACATGAGAGACTCAGCTGTGGTCTGCCGGGAGCTGGGCTGTGGGGGGCCCCGACAGC CAGACCCTGCAGCAGGCCGCTTTGGCTGGGGCGCAGGCCCCATCTGGCTGGATGATGTAGGTTGTGTGGG GACGGAGGCTTCACTGTCAGAATGCCCTGCTGCTTCCTGGGGGAAACACAATTGCGCCCACAATGAGGAT GTCGGGGTTACCTGCACCGGGACCCCCGGCCTGGACACCATCTCAGACCCTTTCAGTTGGAGCTGGCTCC CTGGGCTGGGTAGAGATCAGGATGCCTGGCTCCCAGGAGAGCTGGCCACCAAGCCTTCTGCCAGTCTCAC CTCCAGTGTGCTACAGAAAACCACAAAGGCTCCAGGGAAAGTGCCCAAGAGTACCAAGAAGTGGGTGACT AAAAATGCAAAGAGACCAACCACTCAGCCCCCTGGGATACCAATCACTAAACGCCCCAGGGTCCCAGGCA CCCCAACCTCCCTAAGCCCAACCCCACGTACCTCTGAGTTACCAAAGAGACTTACTGCCGAGGCTCCTCG CAGACAGACCTCACACACCACCGAAAGGCTGACCCCTAGGGTCCCCTGGGAGTGGACCTCAGAGACCGCG GCATCACTGTCCACTCAAGGCCCCCAAGAAGTGACCTCTGAGGCCACCACCGCACAAACCCCTCAGACCT CCTTGGAGCCATTTGGTGAGAACCCAGAAGGCTCTCTAGAATCTTCCCAGGATCCAGCTGCCACCCCGAC TGCTGGAGTTCCCGTGCCATCGGGTCCCTTCCGGGTTCGTCTGGCTGATGGGCCCAACAGGTGTGCTGGC CGGCTAGAGGTGTGGCATGCCGGGCTCTGGGGGACAGTCTGTGATGACAGCTGGGACCTCCGGGATGCCA CAGTGGCCTGCTGGGAACTGGGTTGCGGAAAGGTCCGGCCCCGAGTAGGCAAAACCCACTATGGCCCTGG CACTGGGCCCATCTGGTTGGATGACATGGGCTGTAAGGGAAGTGAGACGTCACTGAGTGACTGCCCCTCG GGGACATGGGGGAAGCACAACTGTGACCACGAAGAAGACGTGGTGCTCACCTGCACTGGCTACACAGGGG ATGACGATTACCCTTCCTGGACCTGGGACCCCACTTCGGAAGAGGACCTGACCAAAAGGACCACAGTGGC CACACAGCCTGGACATACACCTTCCTGGGCTACTACCACAAACCCTGAAGTTCTCTCTCCAGCAACGCAG AGCCTTCCAGACATAGATGACCAGGGTAGTTATGAGCTCTCCTGGACATGGGATACACCTTCAGGAAGGA GTCTGTCCAAAGGGACCCCCACCACAACCAAACCTGGATCCACAGTTACCACTAGCACCAGAAAGAGTCC AGGCCATCCCTTTCCAGCTCCAAGGGCCCGTGCAAACACAGGTTTACCAAGGAAGCCAAAACCTGAGCGC CGGACGCTGCAGCCCACCTCAGCCAGGACGACACACACCACCTCATCCCCGGCTTCCTACACCTCACCAG AGCCTTCTGGCTCACAGCAGACCTCTGGCTCCTGGCCACAACTCTTCCCAGACTTGGCTTCAAAGCAGGA GGGGACGTCTACCCCTCCCAAAACATCTCCACTCACCCCAGGACTTCCCTCCCCAACAAACTTTGCCTTG TCCACTACTGATGCCAGTTTGCTTCCAACACGTCCCCCAGAGCTTTCAGGCAGCCCAACATCCAACTCCC CTGAAGGGCTAACCTCTGACTCCCCCATGCTATTAGAAGTGAGCAGCCTTTCCCCTACCTCAGAGCTGAC GCCAGGATCTGACATGACACCAGACCTGGGCACAGCACCCAAAATAATCCCAGAAAGTTCTGAGTCCTCA GACCTCCCCACGAGCCCCAGCACCCACACACAACCATTCACAGCCTCTTACCCTACCAGCATCCCTCACC CCACCACCATCCCTCATCCCACCACTACCCCTCATCCTAGAGCCCCCTCCCACCCCACCACCATCCCTCA ACTCAACACAACTTCCTACCCCACCATGGCCCTGCAACCTACCACCAACCCTCAGCATCCCGGGAGCCCT CATCCCACCACAAGTCCTCAACCTCCCACCAACACTCACTCTTCTTCAACCTCTGCCACCCCCACAAAGT CCCTTCCATCCTCCCCAGAGACAGAATTTTCCTCTCCTACTCCACAACCAAGGGTCAAGCCTAGCCTACA CCCAGATCTGACCTTTGTAGGAGTCCCGCCCACAGGCAAATCCCAGATGCAGAACCTAGAGTTCTCTCTA GCCTCAGAGTCTGGCCCCTCCAGGCCCTCTCCAGCCCCAAGCATGGACCCACTATCCACAGAGGTCTTCA AACCACCCAGAAGCCAGACCTTACACTCAGTTTCAGAACCCATGGTGACCGCTGACCTTGCCATCTCCTC TATGGCCCACCCATCTGACCAACCTCCCCCAGATCGCCTCGCTCAAGGGCCAACTTCTAATCACAACCCA GGTCCCTTTGGGCCATGTGTCTCCTCAGTACCACCTGTAAGGGTCATGGCTTGTGAGCCACCTGCCTTGG TGGAGCTGGTGGCTGCTGTGAGGGAGGTGGGTGACCAGCTGCAGAGGCTGACCCGGGTCCTGGAGCAGGA CCGGCAGGAGCGCCAAGTCCTGGGGCTGGGCCTAGTCAAGTTGGTAGAGGCTGCCCAGGGGTTGGGGCAG CTAAGTGAGACTGTAAAGAGGCTTGCAGAGGTGGCCTGGCCCCCCAGCACACCTGTGCCCACAACCACCA CCACAGAGGAGGAGGAGAGGCCTCTGAGAGGAGATGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134545
Insert Size	4311 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134545.1, NP_001128017.1
RefSeq Size	4495 bp
RefSeq ORF	4311 bp
Locus ID	308341

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