Chd1 (NM_001107465) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204748 representing NM_001107465 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGACACAGTGATGAAGAAAGTGTTAGAAATGGCAGCGGAGAATCAAGTCAGTCAGAAGATGATT CTGGGTCAGCATCAGGCTCTGGATCTGGCTCAAGCTCTGGCAGCAGCAGTGATGGGAGCAGCAGCCAGTC TGGGAGCAGCGACTCTGATTCTGGTTCTGACTCCGGAAGTCAATCAGAGTCTGAGTCAGACACATCCCGA GAGAACAAAGTTCAAGCAAAACCACCAAAAGTTGATGGAGCTGAGTTTTGGAAATCTAACCCCAGTATTC TGGCTGTTCAGAGATCTGCAATGCTTAGGAAGCAGACACAGCAGCCTCAGCAGCAGCGCCCAGCTTCATC TAATAGTGGATCCGAAGAGGATTCCTCTAGCAGTGAAGACTCAGACGCCTCGTCCAGCGATGTCAAGAGG AAAAAGCATAACGATGAAGATTGGCAAATGTCTGGGTCTGGATCTCCATCTCAGTCTGGTTCAGACTCAG AATCTGAAGACGAGAGAGATAAAAGTAGCTGTGATGGGACAGAGTCTGATTATGAGCCAAAAAACAAAGT CAAAAGCAGAAAACCTCAGAATAGATCTAAGTCAAAAAGTGGGAAAAAAATTCTTGGACAGAAAAAGAGG CAGATTGATTCGTCTGAGGATGAAGATGATGAGGACTACGATAATGATAAACGAAGCTCTCGACGCCAGG CCACCGTCAATGTGAGCTACAAGGAGGATGAAGAGATGAAAACTGACTCTGATGACCTGCTGGAGGTCTG CGGAGAGGACGTCCCTCAGACGGAGGATGAGGAGTTTGAGACCATAGAAAGGTTTATGGATTGCAGAGTT GGGCGGAAAGGAGCTACTGGTGCTACTACAACCATTTATGCTATTGAAGCAGATGGTGACCCAAATGCAG GATTCGAAAAAAGCAAAGAGCCGGGAGACGTCCAGTATTTAATTAAGTGGAAAGGGTGGTCGCACATCCA TAACACGTGGGAGACGGAAGAAACCCTCAAGCAGCAGAACGTTAGAGGGATGAAAAAGTTGGATAATTAT AAGAAAAAAGATCAGGAGACAAAACGATGGCTGAAAAATGCTTCTCCGGAAGATGTGGAATATTACAACT GCCAGCAAGAGCTTACCGATGACCTCCACAAACAGTACCAGATAGTGGAGCGCATAATTGCTCATTCCAA TCAGAAGTCAGCAGCTGGTCTTCCTGATTACTATTGCAAATGGCAGGGGCTTCCCTACTCTGAGTGCAGC TGGGAGGACGGAGCGCTCATTTCCAAAAAGTTCCAGACGTGTATCGATGAGTATTTTAGCAGGAATCAGT CAAAAACTACACCTTTTAAAGATTGCAAAGTACTGAAACAAAGACCAAGATTTGTAGCTCTGAAGAAACA ACCATCCTATATTGGAGGACATGAGGGTTTAGAATTGAGAGACTATCAGCTGAATGGTTTAAACTGGCTT GCTCACTCTTGGTGCAAAGGAAATAGTTGCATACTTGCTGATGAAATGGGCCTTGGAAAAACGATACAGA CCATCTCATTTTTGAACTATTTGTTTCATGAACATCAGTTATACGGACCTTTTCTACTAGTTGTCCCGCT CTCCACTCTGACTTCCTGGCAAAGGGAAATTCAGACGTGGGCGTCTCAGATGAATGCTGTGGTTTATTTA GGTGACATTAACAGCAGAAACATGATAAGAACGCACGAATGGATGCATCCCCAGACCAAACGGTTAAAGT TTAACATACTTTTAACAACGTATGAAATTTTATTGAAGGATAAGGCATTCCTTGGTGGTCTGAATTGGGC ATTTATAGGTGTTGATGAAGCACATCGATTAAAAAATGATGATTCCCTTCTATATAAAACTTTAATTGAT TTCAAATCTAATCACCGCCTCTTAATCACTGGAACTCCTCTGCAGAACTCCCTGAAAGAGCTCTGGTCAT TGCTGCATTTCATTATGCCAGAAAAGTTTTCTTCATGGGAAGATTTTGAGGAAGAACATGGTAAAGGCAG AGAATATGGTTATGCAAGCCTCCACAAGGAGCTTGAGCCATTTCTGTTACGCAGAGTTAAGAAAGATGTG GAAAAATCTCTTCCTGCCAAGGTGGAGCAGATTTTAAGAATGGAAATGAGTGCTTTACAGAAACAATATT ACAAGTGGATTTTAACTAGGAATTACAAAGCCCTCAGCAAAGGTTCCAAGGGCAGTACCTCAGGCTTTTT GAACATTATGATGGAGCTAAAGAAATGTTGTAACCATTGCTACCTCATTAAACCACCAGATAATAACGAA TTCTATAATAAACAGGAGGCCTTACAACACTTAATCCGTAGTAGTGGAAAATTAATTCTTCTAGACAAGC TGTTGATTCGTCTAAGAGAAAGAGGCAATCGAGTCCTCATTTTTTCTCAGATGGTGCGGATGTTAGATAT ACTTGCAGAATATTTGAAGTATCGCCAATTCCCCTTTCAAAGATTAGATGGATCAATAAAAGGGGAGCTG AGGAGACAAGCTCTAGACCACTTTAATGCGGAGGGGTCAGAGGATTTCTGCTTTTTGCTCTCTACCCGAG CTGGGGGTTTAGGCATTAACTTAGCCTCTGCTGACACTGTTGTTATATTCGATTCGGATTGGAATCCACA AAATGATCTTCAGGCTCAAGCTAGAGCTCATCGAATTGGGCAGAAGAAACAGGTGAATATATATCGCTTG GTTACAAAGGGATCCGTTGAAGAAGATATTCTTGAAAGGGCCAAAAAGAAAATGGTCTTGGATCATCTTG TGATTCAACGAATGGATACCACTGGGAAGACAGTGTTGCACACAGGCTCCGCTCCATCAAGTTCTACCCC CTTCAATAAAGAGGAATTATCAGCCATTTTAAAGTTTGGTGCTGAGGAGCTTTTTAAGGAGCCTGAAGGA GAAGAGCAGGAGCCCCAGGAGATGGATATAGATGAAATCTTGAAGAGAGCTGAGACTCACGAAAATGAGC CAGGCCCGCTGACTGTCGGGGATGAGCTGCTCTCCCAGTTTAAGGTTGCCAATTTTTCAAATATGGATGA AGATGACATTGAATTGGAACCTGAAAGAAATTCAAAGAACTGGGAGGACATCATTCCAGAAGAGCAAAGA CGACGGTTAGAAGAGGAGGAGAGACAAAAGGAGCTGGAAGAAATTTATATGCTCCCGAGAATGAGAAACT GTGCAAAGCAGATAAGTTTCAATGGAAGTGAAGGGCGGCGGAGTAGAAGCAGAAGATACTCGGGATCTGA TAGTGATTCAATCTCGGAAAGGAAGCGGCCGAAGAAACGTGGGCGACCACGCACCATCCCTCGGGAGAAC ATTAAAGGCTTTAGTGATGCGGAGATTAGGCGGTTTATCAAGAGCTATAAGAAATTTGGTGGCCCTCTGG AGAGGTTAGATGCAATTGCTCGAGATGCTGAATTGGTTGATAAATCGGAAACAGACCTCAGAAGACTAGG AGAACTGGTGCATAATGGTTGTGTTAAAGCTTTAAAAGATAGTTCTTCGGGAACAGAGCGAGCAGGTGGC AGACTTGGAAAGGTGAAGGGGCCAACGTTCCGAATATCCGGAGTACAGGTGAATGCGAAGCTGGTCATTG CTCACGAGGACGAGCTGATACCTTTGCATAAGTCCATTCCCTCAGACCCGGAGGAGAGGAAGCAGTATAC AATCCCATGCCATACAAAGGCTGCTCACTTCGATATAGACTGGGGCAAAGAGGATGACTCGAATTTATTA ATTGGTATCTATGAATATGGCTATGGAAGCTGGGAAATGATTAAAATGGATCCAGACCTCAGCTTAACAC ACAAGATTCTTCCAGATGATCCTGATAAAAAACCACAAGCAAAACAGTTACAGACTCGAGCAGACTACCT CATCAAACTACTTAGCAGAGATCTTGCAAAAAAAGAGGCTCAGAGGCTTTCTGGTGCGGGAGGCTCAAAG AGGAGAAAAACAAGAGCTAAAAAGAATAAAGCAATGAAGTCCATCAAAGTCAAAGAGGAAATAAAGAGTG ACTCATCGCCCCTGCCTTCAGAGAGGTCTGAGGAGGAGGACGATAACGTGAATGACTCCAAGCCTGAAAG TAAAGACCGCTCCAAAAAGTCTGTGGTGTCTGATGCTCCCGTCCACATCACTGCAAGTGGAGAGCCAGTC CCCATAGCTGAAGAGTCTGAAGAGCTGGATCAGAAGACATTCAGTATTTGTAAAGAAAGAATGAGACCGG TGAAGGCAGCTTTGAAACAACTTGACAGGCCTGAGAAGGGCCTTTCGGAAAGAGAGCAGCTGGAACACAC TAGACAGTGTTTAATCAAGATCGGCGACCATATCACTGAATGTTTGAAGGAGTATTCCAATCCTGAGCAA ATTAAGCAGTGGAGAAAAAACCTGTGGATTTTTGTTTCTAAGTTTACTGAATTTGATGCAAGGAAATTAC ATAAATTATATAAGCATGCTATTAAAAAACGACAAGAATCTCAGCAAAACAGTGACCAGAATAGCAATGT TCCTACCACTCATGTGATTAGGAATCCAGACATGGAAAGGTTAAAAGAGAATACAAATCACGATGACAGC AGCAGGGACGGCTACTCTTCGGACAGACACTTATCTCAGTGCCATGATCATCACAAGGACCGCCATCAGG GAGACTCTTATAAAAAGAGTGACTCTCGGAAGAGACCCTACTCCTCATTTAGCAACGGCAAAGACCATCG CGAGTGGGATCACTACAGGCAAGACAGTAGGTACTATAGTGACAGAGAGAAACACAGAAAACTGGATGAC CACAGGAGTCGAGAGCACAGGCCAAGTGTGGAAGGCAGCTTAAAGGACAGGTGTCATTCTGACCACCGAT CTCACTCGGACCATCGGATGCATTCAGACCATCGCTCCAGCACTGAGCACACACACCATAAGTCTTCCAG GGATTATAGGTACCTCTCAGATTGGCAGCTGGACCACCGAGCTGCCAGCAGTGGCCCTAGGTCTCCTTTA GATCAGAGGTCTCCATATGGCTCCAGGTCCCCATTTGAACATTCAGCTGAACACAGAAGTACACCTGAAC ACACCTGGAGTAGTCGGAAGACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107465
Insert Size	5136 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107465.1, NP_001100935.1
RefSeq Size	6568 bp
RefSeq ORF	5136 bp
Locus ID	308215

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