Kdr (NM_013062) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Vegfr-2
Vector	pCMV6-Entry2
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204835 representing NM_013062 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGCAGGGCGCTGCTAGCTGTCGCTCTGTGGTTCTGCGTGGAGACCCGAGCCGCCTCTGTGGGTT TGCCTGGCGATTCCCTCCATCCACCCAAGCTCAGCACACAAAAAGACATACTTACAATTTTGGCAAATAC AACCCTTCAGATTACTTGCAGGGGACAGAGGGACCTGGATTGGCTTTGGCCCAACACTCCGCGTGACTCT GAGGAAAGGGTGTTGGTGACTGAGTGTGGCGACAGTATCTTCTGCAAGACACTCACAGTTCCCAGAGTGG TTGGAAATGATACTGGAGCCTACAAGTGCTTCTATCGGGACACCGATGTCTCCTCCATCGTTTATGTCTA TGTTCAAGATCACAGGTCACCATTCATCGCCTCTGTCAGTGACGAGCATGGCATCGTGTACATCACTGAG AACAAGAACAAAACTGTGGTGATCCCATGCCGAGGGTCGATTTCAAACCTCAACGTGTCACTTTGTGCTA GGTATCCAGAAAAGAGATTTGTTCCGGATGGAAACAGAATTTCCTGGGACAGCGAGAAAGGCTTTACTAT CCCCAGTTACATGATCAGCTATGCCGGCATGGTCTTCTGTGAGGCAAAGATTAATGATGAAACGTATCAG TCTATCATGTACATAGTTCTGGTTGTAGGATATAGGATTTATGATGTGGTCCTGAGCCCCCCTCATGAAA TTGAGCTATCTGCCGGAGAAAAGCTTGTCTTAAATTGTACAGCAAGAACAGAGCTCAACGTGGGGCTTGA TTTCAGCTGGCAATTCCCGTCCTCAAAGCATCAGCATAAGAAGATTGTAAACCGGGATGTGAAATCCCTT CCTGGGACTGTGGCAAAGATGTTTTTGAGCACCTTGACCATAGACAGTGTGACCAAGAGTGACCAAGGAG AATACACCTGCACAGCGTACAGTGGACTGATGACCAAGAAAAATAAAACATTTGTCCGAGTTCATACAAA ACCTTTTATTGCTTTTGGTAGCGGGATGAAATCTTTGGTGGAAGCCACTGTGGGCAGCCAAGTCCGAATC CCTGTGAAGTATCTCAGTTACCCAGCTCCTGATATCAAATGGTACAGAAATGGACGACCCATTGAGTCCA ATTACACAATGATCGTTGGTGATGAACTCACCATCATGGAAGTGAGTGAAAGAGATGCGGGAAACTACAC GGTCATCCTCACCAATCCCATTTCAATGGAGAAACAGAGCCACATGGTCTCTCTGGTTGTGAATGTTCCA CCCCAGATCGGTGAGAAAGCCTTGATCTCTCCTATGGATTCCTACCAGTATGGCACCATGCAGACGCTGA CATGCACAGTCTATGCCAACCCTCCCCTGCACCACATCCAATGGTACTGGCAGCTAGAAGAAGCATGCTC CTACAGGCCCAGCCAAACAAACCCATATACTTGTAAAGAATGGAGACACGTGAAGGATTTCCAGGGGGGA AATAAGATCGAAGTCACCAAAAACCAATATGCCCTAATTGAAGGAAAAAACAAAACTGTAAGTACTCTGG TCATCCAGGCTGCCTACGTGTCCGCATTATACAAATGTGAAGCCATCAACAAAGCAGGACGAGGAGAGAG GGTCATCTCCTTCCATGTGATCAGGGGTCCTGAAATTACTGTCCAGCCTGCTACCCAGCCAACCGAGCGG GAGAGTATGTCTTTATTGTGCACTGCAGATAGAAACACGTTTGAGAACCTCACGTGGTACAAGCTTGGCT CACAGGCAACATCGGTCCACATGGGCGAATCACTCACACCAGTTTGCAAGAACTTGGACGCTCTTTGGAA ACTGAATGGCACCGTGTTTTCTAACAGCACAAACGACATCTTGATTGTGGCATTCCAGAATGCCTCCCTG CAGGACCAAGGCAACTATGTCTGCTCTGCTCAAGACAAGAAGACCAAGAAAAGACATTGCCTAGTCAAGC AGCTCGTCATCCTAGAGCGCATGGCACCCATGATCACTGGAAATCTGGAGAATCAGACAACAACCATTGG TGAGACCATCGAAGTTGTTTGTCCAACATCTGGAAACCCTACCCCCCTCATTACATGGTTCAAAGACAAT GAGACCCTTGTAGAAGATTCAGGCATTGTACTAAAAGACGGGAACCGGAACCTAACTATCCGAAGGGTGA GGAAGGAAGACGGGGGCCTCTACACCTGCCAGGCCTGCAATGTCCTTGGCTGTGCAAGAGCAGAGACACT CTTCATAATAGAAGGCGTCCAGGAAAAGACCAACTTGGAAGTCATTATCCTCGTCGGCACTGCAGTGATT GCCATGTTCTTCTGGCTCCTTCTTGTCATTCTCGTACGGACCGTTAAGCGGGCCAATGAAGGGGAACTGA AGACAGGCTACTTGTCCATTGTCATGGATCCAGATGAACTGCCCTTGGATGAGCGCTGTGAACGCTTGCC TTATGATGCCAGCAAGTGGGAGTTCCCCAGGGACCGGCTGAAACTAGGAAAACCTCTTGGCCGTGGTGCC TTTGGCCAAGTGATTGAGGCAGATGCCTTTGGAATCGACAAGACAGCGACTTGCAAAACAGTGGCTGTCA AGATGTTGAAAGAGGGAGCAACACACAGCGAGCACCGAGCCCTCATGTCCGAACTCAAGATCCTCATCCA CATTGGCCACCATCTCAATGTGGTGAACCTGCTGGGTGCCTGCACGAAGCCCGGAGGGCCTCTCATGGTG ATTGTAGAATTCTGCAAGTTTGGAAACCTATCAACTTACTTACGGGGCAAGAGAAATGAATTCGTGCCCT ATAAGAGCAAAGGGGCACGCTTCCGCTCTGGGAAAGACTATGTTGGGGAGCTCTCCGTAGACCTGAAGCG GCGCTTGGACAGCATCACCAGCAGTCAGAGCTCTGCCAGCTCAGGTTTTGTGGAGGAGAAATCCCTCAGT GACGTAGAGGAAGAAGAAGCTTCTGAAGAACTGTACAAGGACTTCCTGACCTTGGAGCATCTCATCTGTT ACAGCTTCCAAGTGGCTAAGGGCATGGAGTTCTTGGCATCAAGGAAGTGTATCCACAGGGACCTGGCAGC ACGAAACATTCTCCTATCGGAGAAGAACGTGGTTAAAATCTGTGACTTTGGCTTGGCCCGGGACATTTAT AAAGACCCAGATTACGTCAGAAAAGGAGATCCCCGACTCCCTTTGAAGTGGATGGCTCCGGAAACAATTT TTGACAGAATATACACAATTCAGAGTGGTGTGTGGTCTTTTGGTGTTTTGCTCTGGGAAATATTTTCCTT AGGTGCTTCCCCATATCCTGGGGTCAAGATTGATGAAAAATTTTGTAGGAGATTGAAAGAAGGAACGAGA ATGCGGGCTCCTGACTACACCACCCCAGAAATGTACCAAACCATGCTGGATTGCTGGCATGAGGACCCCA ACCAGAGACCCGCGTTTTCAGAGTTGGTGGAGCACTTGGGAAATCTCCTGCAAGCAAATGCTCAGCAGGA TGGCAAAGACTATATTGTTCTTCCAATGTCAGAGACACTGAGCATGGAAGAGGATTCTGGACTCTCCCTG CCTACCTCACCTGTTTCCTGTATGGAGGAAGAGGAAGTGTGCGACCCCAAATTCCATTATGACAACACAG CAGGAATCAGTCATTATCTGCAGAACAGCAAGCGAAAAAGCCGGCCAGTGAGTGTAAAAACATTTGAAGA TATCCCTTTGGAGGAACCAGAAGTAAAAGTGATTCCAGATGACAGCCAGACAGACAGTGGGATGGTCCTT GCCTCAGAAGAGCTGAAAACTCTGGAAGACAGGAACAAATTATCTCCATCTTTTGGTGGGATGATGCCCA GTAAAAGCAGGGAGTCTGTGGCCTCGGAAGGCTCCAACCAGACCAGCGGCTACCAGTCTGGGTATCACTC AGACGACACAGATACCACCGTGTACTCCAGCGACGAGGCAGGACTTTTAAAGCTGGTGGATGTTGCAGGG CACGTTGACTCTGGGACCACACTGCGCTCATCTCCTGTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_013062
Insert Size	4032 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013062.1, NP_037194.1
RefSeq Size	5892 bp
RefSeq ORF	4032 bp
Locus ID	25589
UniProt ID	O08775
Gene Summary	receptor for vascular endothelial growth factor (VEGF) [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.