Tubgcp6 (NM_001108748) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204992 representing NM_001108748 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCATCACCCAGCTGTTCGATGACCTCTGTGAGGCTCTCTTACCTGCTGCCCAGGCTCGCCCCG GCCAGCGCAGCGTGAACAGGAAAAAGGCAAAGCTGAGTCTTAAAAGGGTAGCCTACAATGCTCTTTTCGC AAATCTTTTTCAAGAGGACACCCATCAGCGGCAGCCTGACTCGAAGCTCCCTGTGAAAAACAAGGTCCTC ATGCTGTCCTTCGATTTGAGGGTCGGGGGCTTGGGGCCAGAGGCTGACCGTTTGGAGGAGCTTGTAGAGA AACTTGAAACAGCCCCTGACTGTCCTTTTGTGGAAGTGGGATCAGTGTTGGACCTCTTAGTTCAGCTGGC AGGAAGTGGCCCCCCTCAAGTGCTGCGCAGGAAACGGGATTACTTCTTTAATAACAAGCACGCAGGCCGG AACATACCATACAGCGGCTACGATTGCTATGACTTGAGTGTGTTTGAAATGGACGTTCGGTCACTCATCT CTGGGGAGGAGAATTTGTGTCATAACATGGTCCAGGAAGCCCTTCAGGTGATGGAGGCTGCCCCAGGCAC TGGCCTGACCACCGTTGGGCTCTTCTCAACTGGTGACTCTTGTGGTGACAGGTTTGAGAGAGACACTAGG GTCTCTCTCTTCGGTGCACTTGTGCATAGCCGCACATATGACATGGATGTGCGACTGGACCTGCCCCCTG TGCCAGACAGCGCCGACTTCTCTGGACTGGCCATCAAGGTCCCACAGATTGTGGATCAGTGGGAAGATGA AGGTTTCCAATCAGCTTCCAACTTAACTCCCGACTCCCAGTCTGAACCAAGCATGACCCCGGATTTGGAC CTCTGGGAAGCTGTGCTCACTTATGAAGCCAGCAAGCGGAGGTGCTGGGAGCGTATTGGATGTCCCCCTG GTCACCGAGAGGAGCCCTACCTCACTGAGGCAGGAAGGGATGCCTTTGACAGGTTCTGCAGGCTCTGTCA TGGAGAACTACAGGCCCTCAGTGGGGGCCTCCTCCAGACCCCAAGGCCTGTGCTGGTGGAGGAAAGCGAG CTGGTGAAAGACTCACTGAACGTTCTGCTGGGTGTTGTCTCTGCTACATTTTCCCTCTGTCAGCCGACTC AAGCTTTTGTAGTGGAGCCGGGCGTGCACGTGTCAGGTGCATCTCCTGAAAGCATCAGCAGCATCCTTTC AGAGGTGGCGGAGTATGGGACCTGTTATACACGCCTGAGCCACTTCTCTCTGCAGCCTGTGGTAGGCTCC TTGTGCAGCAGGGGCCTCGTGTTCCAGGCCTTCACCAGCGGCCTCAGGAGGTACTTGCAGTATTACCGCG CCTGTGTTCTCTCCACTCCACCTACCCTAAGTCTCCTCACCATTGGCTTTCTCTTCAAGAAGCTAGGCCG GCAGCTCAGGTACTTGGCTGAGCTTTGTGGTGTTGGCACTGTATCCCTGGCGACCAGTGGAGAACCCAGG GCTGTGTTCCCCACTGGGGTGAAGCTTCTGTCCTACCTCTACCAGGAAGCACTAGATAACTGCAGTAATG AACACTATCCTGTGTTGCTGTCCCTGTTGAAAACCAGCTGTGAGCCCTACACACGGTTCATCCATGATTG GGTGTACAGTGGTGTCTTCAGAGATGTTTATGGGGAGTTCATGATCCAAGTTAACCACGAGTACCTTAGC TTCAGAGACAAGTTTTACTGGACCCATGGCTACGTGCTCATTTCCAAAGAGGTAGAGGACTGTGTTCCTG TGTTCTTGAAACACATTGCTCACGATGTGTATGTCTGTGGAAAGACCATCAACCTACTGAAGCTCTGCTG TCCCCGGCATTACCTTTGTTGGTCTGATGTCCCTGTGCCTCGGATCTCCGTGATCTTCTCTCTCGAGGAA TTGAAGGAGATTGAGAAGGACTGTGCCGTCTATGTTGGGCGGATGGAGAGAGTTGCACGTTATAGTTCTA TCAGCAAGGAGGAAAAGGAACTACGTATGGAAATTGCAAAACAAGAACTAATTGTCCATGCCCGGGAAGC AGCATCCAGGGTCCTGAGTGAGCTGAGTGACCGGCAGATGTCAGAACAGATTGCTCTGGACACCCGGAAG CGAGAGCAGTTTCAGCGACTGAAGGAACAGTTTGTGAAGGATCAGGAGAGACGCCTGGCAGCCAGACAAG AAGAGCTCGGTGATGACTTCAGTTATGCCCATGAACTCCGTGCCCGGGAGAAGAGGCTAAAGGCCCTGGA GGAAGAACTGGAGAGGAAGGCCAGGCAGGCGCTGGTTGATCACTATAGCAAGTTGTCTGCAGAGGCAGCT CGTCGAGAGCAGAAGGCTCTGTGGAGAATCCGGAGGCATCGGCTGGAAAGTGCACGACTTCGTTTTCTCC TGGAAGACCAGAAGTGTATTCAGGAGATGCTGAGAGACATGGAGGCTCACCAGCCCCAGGAGCCACTGAG TGTTCTCCCAAGCACATGCTCTCAGGTCACGTCTCTCGGTCCCGAGCATGAAGGTGAAGGCCATAGCTGT GATCTGGAGTCCACAGAACTGCGCTGCGATTGTTCAAACCAACCATGTGCATCAACACCATCGGTCCTCA AGTCTGGGGCTGGACAGGCAGAGGAAGCTGGGCCATTCTCAACTGGTCTCAGCATCACAGACTTTCTGCC CATGGGCTCTGGAGATGAACAGCCTGTGGGTGACACTGGAGCTCCCTTCTTAGAGGTGGCTCTGCAGAGC ATCTGCTCAGACCTATCCCCTATAACCCCTGGACCGGCACTCACAGCAGGTGGCCTGCAGCCTACCCAGT CAGAGTATGATTTCAATACCATACTGAGGCCAGCTATGGCTACCCCCCCTTTACCGGGACCCTTCCGGGA TGTTCAGAATAGTGTAGACAGTGAAAAACAGCATCTACTGCGGGATATGCCCACAAAACCAGATTCATGT ATCCATGACATGCAAGAGACTTTGCCTTGCCTACATCCACTCAGTCATGTCACTCCTGCGGAGGGCAGCC TCCAGCCTGTGGGCCAGCTCCTTGAACACATGTCAGAGACTACTGTCGCTACAGAGAGCCATGCTTCTGG AATGGCTCCTTGCCAGCAACTTAGCATTTCCAGACACGTGTCAGATGCCAACATCAAGGTGGGAGATTAT ATGTCAGACGTGGCTCTTCCTCGGCCACGGTGGAATATCCATGGACATGTGTCAGAGGCCAACATTGGAG TTGGGGAGAATGTGTCGGACGTGGCTCCTTCTCGGCCACGGTGGAATGTCCACGGACATGTGTCAGATGC CAGCATCAAGGTTGGGGAGAATGTGTCAGACGTGGCTCCTTCTCGGCCACGGTGGAATGTCCACGGACAT GTGTCCGATGCCAGCATCAAGGTTGGGGAGAATGTGTCAGAAGTGGCTCCTTCTCGGCCACGGTGGAATG TCCACGGACATGTGTCAGATGCCAGCATCAAGGTTGGGGAGAATGTGTCAGACGTGGCTCCTTCTCGGCC ACGGTGGAATGTCCACGGACATGTGTCAGATGCCAGCATCAAGGTTGGGGAGAATGTGTCAGACGTGGCT CCTTCTCGGCCACGGTGGAATGTCCACGGACATGTGTCAGAGGCCAGTATTAGGATTGGGGAGAATGTGT CAGATGTGGATCTTCAGCCACATGGACATGTTCAGCCTCCCTTGATTCTGGAGGAGCCCTTCCCAGAAGC TGAGCCTGACCTCAAGTCCCATCAGTGTCCCCCTGATCATGTGTCTCAGACAGTCCTTTGTGTGGAAGCA CAGAGCCCTGCTCAAGAATGTGAGCCTCAACTACCTGAAGAAATGAAGCCTACCATCTGTTTGAGCCTTG GCAGTACAGAGGAAGGTAGCCTCCAGACCAAGACCCTTGTGACTGAGCCTAGCATGCTGGGAAATGGCAT CCCTGAAGAGAAAGGCCCAAGGAGGAATAGGGATGCTGAAGACCTTTCTCCATGTTTACCTTCAAGCCCA CAGGAAGACACACTCCCCAGTAGCCCAGGTCCTAGTGAGGAGGTACCAAGCACAGAAGCGGAGGAGGCCA GACGCTGGGGCAAGGAGCAAGCCTATTTGGCAGATCTCATGAAACTGTACCGCCTGGAGCAGTACCCGGA CAGCTACGAGTCCATGTCGGAACCTCCCATGGCTCACTTGGTACACCACATGCTTCCCCGGGCCTTCGCC TTCCCTGTTGACCCCCAAGTGCAGTCAGCAGTGGATGAGAGTGCAGTGCAGCTGAGTGAGCTGCTGACGT TGCCTGTGCTTATGAAGCGCTCCCTCATGGCTCCTTTGGCTGCCCATGTCAGCTTGGTCAACAAAGCTGC CGTCGACTACTTCTTTGTGGAGCTGCACCTGGAGACACACTTTGAGGCACTGCGGCATTTCCTGCTGATG GAGGATGGAGAGTTCGCTCAGTCCCTCAGTGACCTGCTTTTTGAGAAGCTAGGGGCTGGGCAGACGCCTG GAGAGCTGCTCAACCCACTGGTCCTTAACAGCATCCTGAGCAAGGCACTGCAGTATAGCCTCCACGGGGA CACCCCACATGCTACCAACCTGTCTTTTGCCCTCAAGTACCTGCCTGAGGTGTTTGCCCCTAATGCCCCA GATGTGCTGAGCTGCCTGGAGCTCAGGTACAAGGTCGACTGGCCCCTCAACATTGTTATCACCGAGAGCT GCCTGAACAAGTACAGTGGGATCTTCTCCTTCCTGTTGCAGTTGAAGCTCATGATGTGGACACTCAAAGA CATCTGCTTCCACCTCAAGCGCACAGCCCTAGTGAGCCACACAGCTGGCTCAGTGCAGTTCCGACAGCTG CAGCTGTTTAAGCATGAGATGCAGCATTTTGTGAAAGTCATCCAGGGCTACATTGCTAACCAGATCCTGC ACGTCAGCTGGTGTGAGTTCAGAGCCCGTCTAGCTGTGGTGGGCGACCTGGAGGAGATTCAGCGGGCCCA TGCCGAGTACCTACACAGGGCTGTTTTCAGGGGCCTACTGACCGAGAAGGCAGCACCCGTCATGAACATC ATCCATAGCATCTTCAGCCTGGTGCTCAAGTTCCGAAGCCAGCTCATCTCTCAGAACTGGGGCCCGGCTA CCGGCCCCCGTGGTGCCGAGCACCCCAACTTCCCACTCATGCAGCAGTCCTACAGCACCTTCAAGTACTA CTCCCACTTCCTCTTCAAAGTAGTGACCAAGCTGGTGAACAGAGGCTATCAGCCCCACCTGGAAGACTTT TTGCTTCGGATCAACTTCAACAACTACTACCAGGACTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108748
Insert Size	5292 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108748.1, NP_001102218.1
RefSeq Size	6445 bp
RefSeq ORF	5292 bp
Locus ID	362980

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