Itga1 (NM_030994) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205013 representing NM_030994 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCCCAGGCGTCCTGCCAGCCTAGAGGTCACTGTAGCCTGCATATGGCTTCTCACGGTCATCCTAG GCTTCTGCGTCTCCTTCAATGTTGATGTGAAAAACTCAATGAGTTTCAGTGGCCCAGTAGAGGACATGTT TGGATACACTGTTCAACAATATGAAAACGAAGAAGGCAAATGGGTTCTTATTGGTTCTCCTTTAGTTGGC CAACCCAAAGCAAGAACTGGAGATGTCTATAAGTGTCCGGTTGGGAGAGAGAGAGCAATGCCTTGCGTGA AGTTGGACTTGCCAGTTAACACATCGATCCCCAATGTCACAGAAATAAAGGAAAACATGACATTTGGATC AACTTTAGTCACCAACCCGAATGGAGGATTTCTGGCATGTGGGCCCTTGTATGCCTATAGATGTGGACAT TTGCATTATACAACTGGAATATGTTCTGATGTCAGTCCTACATTTCAAGTTGTGAACTCCTTTGCCCCTG TACAAGAATGCAGCACCCAGCTGGACATAGTCATCGTCCTGGATGGCTCCAACAGCATCTACCCCTGGGA AAGTGTCATCGCCTTTTTAAACGACCTTCTTAAGAGGATGGATATTGGCCCTAAGCAGACACAGGTCGGG ATTGTACAGTATGGAGAGAATGTAACCCATGAGTTCAACCTCAATAAGTATTCATCCACAGAAGAGGTCC TTGTCGCAGCAAACAAAATAGGCCGACAGGGAGGCCTCCAAACGATGACAGCCCTTGGAATAGACACAGC CAGGAAAGAGGCATTCACTGAAGCTCGGGGTGCCAGGAGGGGAGTTAAAAAAGTCATGGTTATTGTGACC GACGGAGAATCGCATGACAACTATCGCCTGAAACAGGTCATCCAAGACTGCGAGGACGAAAACATTCAGC GATTTTCCATAGCTATCCTTGGCCACTATAACAGGGGGAACTTAAGCACTGAAAAATTTGTGGAGGAAAT AAAATCGATCGCAAGCGAGCCCACGGAAAAGCACTTCTTCAATGTCTCGGATGAGTTGGCCCTGGTCACT ATTGTTAAAGCTCTGGGAGAAAGGATATTCGCTTTGGAAGCGACAGCTGACCAGTCAGCAGCTTCATTTG AGATGGAAATGTCTCAGACTGGCTTCAGTGCTCACTACTCCCAGGACTGGGTCATGCTTGGAGCGGTGGG AGCCTATGACTGGAACGGAACTGTGGTCATGCAGAAGGCTAACCAGATGGTCATCCCTCATAACACCACC TTTCAAACTGAGCCCGCCAAGATGAACGAGCCTCTGGCTTCTTATTTAGGTTACACAGTGAACTCGGCCA CCATCCCTGGAGATGTGCTCTACATCGCTGGGCAGCCTCGGTACAATCATACGGGCCAGGTCGTCATCTA CAAGATGGAGGATGGGAACATCAACATTCTGCAGACACTCGGCGGAGAGCAGATTGGTTCCTACTTTGGT AGTGTCTTAACAACAATTGACATCGACAAAGATTCTTATACTGATCTGCTTCTCGTCGGGGCCCCCATGT ACATGGGGACAGAGAAAGAGGAACAGGGCAAGGTGTACGTGTACGCTGTGAATCAGACAAGGTTTGAATA TCAAATGAGCCTGGAACCAATTAGGCAGACCTGCTGCTCATCCCTGAAGGATAATTCATGCACGAAAGAA AACAAGAATGAGCCCTGCGGGGCCCGCTTCGGAACAGCAATTGCTGCTGTAAAAGACCTCAACGTGGATG GATTTAATGACGTCGTGATTGGAGCTCCGCTGGAAGATGACCACGCAGGAGCTGTGTACATTTATCATGG CAGTGGCAAGACCATAAGGGAGGCGTATGCACAACGCATTCCATCAGGTGGGGATGGCAAGACCCTGAAA TTTTTCGGCCAGTCTATCCACGGAGAGATGGATTTAAATGGTGACGGTCTGACTGACGTGACCATTGGAG GCCTTGGTGGAGCAGCCCTCTTCTGGGCCAGAGATGTGGCTGTAGTTAAAGTGACCATGAATTTTGAACC CAATAAAGTGAATATTCAAAAGAAAAACTGCCGTGTGGAGGGCAAAGAAACAGTGTGCATAAATGCTACA ATGTGTTTTCATGTGAAATTAAAGTCTAAAGAGGACTCAATTTACGAGGCTGATCTGCAGTACCGTGTCA CCCTTGATTCACTGAGGCAGATATCACGGAGCTTTTTTTCTGGAACTCAGGAAAGGAAGATTCAAAGAAA TATCACCGTTCGAGAATCAGAATGCATCAGGCACTCCTTCTACATGTTGGACAAACATGACTTTCAGGAC TCTGTGAGAGTGACTCTGGATTTTAATCTCACTGATCCAGAAAATGGTCCTGTACTTGATGACGCTCTGC CAAACTCAGTCCACGAACACATTCCCTTTGCCAAAGACTGTGGAAACAAGGAAAGATGCATTTCAGACCT CACTCTGAATGTGTCCACCACAGAAAAGAGCCTGCTGATCGTCAAGTCCCAGCATGACAAGTTCAACGTT AGCCTCACCGTCAAAAACAAAGGAGACAGTGCGTACAACACCAGGACAGTGGTGCAGCATTCACCAAATC TGATTTTTTCGGGAATTGAGGAGATCCAAAAAGATAGCTGTGAATCTAATCAAAATATCACTTGCAGAGT TGGATATCCTTTCCTAAGAGCAGGAGAAACGGTTACCTTCAAAATAATATTCCAGTTTAACACATCCCAT CTCTCGGAAAATGCAATCATTCACTTAAGTGCAACAAGTGACAGTGAGGAGCCCCTGGAATCTCTTAATG ATAATGAAGTAAATATTTCCATCCCAGTAAAATATGAAGTTGGACTGCAGTTTTACAGTTCTGCGAGTGA ACATCACATTTCAGTCGCTGCCAATGAGACGATCCCTGAGTTTATTAACTCCACTGAGGACATTGGGAAT GAAATTAATGTCTTCTATACGATTAGAAAGAGGGGGCATTTCCCAATGCCAGAACTTCAGCTGTCAATTT CATTCCCCAATTTGACGGCAGATGGTTATCCTGTACTGTACCCAATTGGATGGTCATCTTCAGATAATGT GAACTGTAGACCCCGGAGCCTTGAGGACCCCTTTGGCATCAACTCTGGGAAGAAAATGACAATATCGAAG TCTGAGGTTCTCAAAAGAGGCACAATCCAGGACTGCAGTAGTACGTGTGGAGTTGCCACCATCACGTGTA GCCTCCTTCCTTCCGACCTGAGTCAAGTGAATGTCTCGCTCCTCCTGTGGAAACCGACTTTCATAAGAGC ACATTTTTCCAGCTTAAACCTTACTCTAAGAGGAGAACTTAAGAGTGAAAATTCATCGCTGACTTTAAGT AGCAGCAACCGGAAGCGAGAGCTGGCTATTCAGATATCCAAAGACGGGCTCCCAGGCAGAGTGCCGCTGT GGGTTATCCTCCTGAGCGCCTTCGCGGGGCTACTGCTGCTAATGCTCCTTATATTGGCTCTGTGGAAGAT TGGATTCTTCAAAAGGCCACTGAAGAAGAAAATGGAGAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_030994
Insert Size	3543 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_030994.2, NP_112256.1
RefSeq Size	3963 bp
RefSeq ORF	3543 bp
Locus ID	25118
UniProt ID	P18614
Gene Summary	alpha subunit of the integrin molecule; involved in binding laminin and collagen [RGD, Feb 2006]

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