Pik3c2a (NM_001108500) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205077 representing NM_001108500 Red=Cloning site Blue=ORF Orange=Stop codon CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGCTCAGATTTCCAACAACAGTGAATTTAAACAGTGTTCATCTTCACATCCAGAACCAATAAGAACCA AAGATGTGGACAAAGCAGAAGCATTACAGATGGAAGCAGAAGCCTTAGCAAAATTGCAGAAGGATAGACA CATGACTGACAGTCCAAGAGGCTTTGAGCTGTCTAACAGCACTAGACAAAGAACACAGGGTTTTAACAAA CAGGATTATGATCTCATGGTGTTTCCTGAGTCGGATTCCCAAAAAAGAGCATTAGATATTGACGTAGAAA AGCTTACCCAAGCTGAACTTGAAAAGATATTGCTGGATGACAATTTTGAAACTAGAAAAACTCCTGCATC GCCAGTTACTCCTGTTCTGAGCCCTTCATTTTCAGCACAGCTATATCTTAGACCTGCTGTTCAAAGAAGC CAGTGGCCCCCTGGATTATGCGGGCCTTCCACATACACTTTACCTTCTATTTATCCTTCAGCATACAGTA AACAGGCCACATTCCAGAATGGCTTCAGTCCAAGGATGCCTACTTTTCCATCTACAGAGTCTATATATTT AAGACTTCCAGGACAGTCTCCATATTTTTCATATCCTTTGACACCTGCCACACCATTTCATCCACAAGGA AGTTTACCAGTCTATCGGCCACTAGTCAGTCCTGACATGGCAAAACTATTTGACAAAATAGCAAGTACCT CAGAATTTTTAAAAAATGGGAAAGCAAGGACTGATTTGGAGATAGCAAACTCGAAAGCTTCAGTCTGCAA TCTACAAATATCTCCAAAGTCTGAAGACATCAATAAATTTGATTGGTTGGACTTGGATCCTCTGAGTAAG CCTAAGATAGACTGTGTGGAAGTGTTAGAACATGAAGAAGAGAAGAAGAATCCAGTTTTGCTAGCAGAGG ATCCTTGGGATGCTGTTCTTCTTGAAGAGAGATCACCAACAAGTCGTCACCTAGAAAGGAAGGTGAATGG AAAATCCCTTTCTGGGGCGACTGTAACAAGAAGCCAGTCTTTAATCGTTCGGACAACTCAAGTTACAAAA GCCCAGAGCCAGGTATCTCAGAAAGACCCAAATGGGACCAGTAGTTTGGCAACTGGAAGTTCTCTTCTAC AAGAATTTGAAGTACAGAATGAGGAGGTGGCAGCTTTTTGTCAATCCATTATGAAATTAAAGACCAAATT TCCATATACCGATCACTGTACAAATCCAGGCTATTTGTTAAGTCCAGTCACAGTGCAAAGAAACATGTGT GGGGAAAATGCCAGTGTGAAGGTCTCCATTGAAATTGAAGGGTTTCAACAACCAGTTACTTTTACATGTG ATGTGAGTTCTACTGTAGACATAATTATAATGCAAGCCCTTTGCTGGGTACATGATGACTTGAATCAAGT GGATGTTGGCAGCTACATTCTGAAAGTTTGTGGTCAAGAGGAGGTTCTACAGAATAATCATTGCCTTGGA AGTCACGAACATATTCAAAATTGTCGAAAATGGGACACAGAGATTAAATTACAACTCTTGACCTTCAGTG CAATGTGCCAGAATCTGGCTCGAACAGCAGAAGATGATGAAGCACCTGTGGATTTAAACAAGTACTTGTA TCAAATAGAAAAACCTTATAAAGAAGTCATGACAAGACACCCTGTTGAAGAACTCTTAGATTCCTATCAC TACCAAGTAGAACTGGCTCTACAAACTGAAAACCAGCACCGAGCTATTGATCAAGTGATTAAAGCAGTAA GAAAAATCTGTAGTGCTTTAGATGGGGTTGAGACCCCCGCCGTCACAGAAGCAGTGAAGAAGCTAAAGCG AGCAGTTAACCTTCCAAGGAACAAAAGTGCTGATGTGACTTCATTATCTGGAGGAGATACTAGCAAGAAC TCAACTAGGGGCTCACTGAATCTTGAAAATCCTGTTCAAGTAAGCATGGATCACTTAACGGCAGCAATTT ATGATCTTCTCAGGCTTCATACAAATTCTTGTAAGAGTTCTACAGTCTGTCCCCAAGGTAGCAGGAACAT CAAGGAAGCATGGACTGCAACAGAGCAGCTCCAGTTCACCGTCTATGCCGCACACGGAATTTCCAGTAAC TGGGTATCCAATTACGAAAAATACTACTTGATATGTTCCCTGTCTCACAATGGGAAGGATCTTTTTAAGC CTATTCAGTCAAAGAAGGTTGGCACATACAAGAATTTCTTCTATCTTATCAAATGGGATGAACTAATCAT TTTCCCTATCCAGATATCGCAGTTGCCTTTAGAGTCAGTTCTTCATCTCACTTTGTTTGGAGTTTTAAAT CAGAGCAGTGGAAGTTCCCCTGATTCTAATAAACAGAGAAAGGGGCCAGAAGCTCTGGGCAAAGTCTCTT TAACTCTATTTGACTTTAAACGGTTTTTAACATGTGGAACTAAACTTCTCTACCTTTGGACTTCATCACA TACAAATTCTATTCCTGGAGCAATCCCCCAAAAAAGCTATGTCATGGAAAGAATTGTGCTACAGGTTGAT TTTCCTTCTCCTGCATTTGACATTATTTATACATCTCCTCAAATTGATAGAAACATTATACAGCAATACA AGCTGGAAGCACTGGAGAATGAAATAAAGGGAAAACTTCTGGATATTGTTCACAGAGATTCATCATTTGG ACTTTCTAAAGAAGATAAGGCCTTTTTGTGGGAAAAACGCTATTATTGCCTAAAACATCCAAATTGTCTT CCGAAGATACTAGCAAGTGCTCCAAACTGGAAGTGGGCTAATCTTGCCAAAACTTACTCTTTGCTCCACC AGTGGCCGCCATTGTGTCCATTAGCTGCATTGGAGCTCCTTGATGCAAAATTTGCTGATCAGGAAGTGCG ATCGCTAGCTGTGAGCTGGATTGAGGCCATTAGTGATGATGAGCTAACAGATCTTCTCCCACAGTTTGTA CAGGCTTTGAAATATGAAATTTATTTGAATAGTTCACTAGTGCGCTTCCTTCTGTCCAGGGCACTGGGAA ACATCCAGATAGCACACAGTTTGTATTGGCTTCTCAAGGATGCTTTGCACGATACACGGTTTGGAAGCAG ATATGAACATGTGTTGGGTGCTCTCCTCTCTGTAGGAGGAAAAGGACTCAGAGAAGAGCTTTCTAAGCAG ATGAAACTTGTACAGCTTTTAGGAGGAGTGGCAGAAAAAGTAAGGCAGGCTAGTGGATCAGCCAGACAGG TGGTCCTCCAGAAGAGTATGGAACGAGTACAGTCCTTTTTTCTGAGAAATAAATGCCGGGTTCCTCTCAA ACCAAGTCTAGTAGCGAAAGAATTAAATATTAAGTCATGCTCATTCTTCAGTTCGAATGCCATGCCTCTG AAAGTCACAATGGTGAATGCTGATCCTCTGGGAGAAGAAATTAATGTCATGTTTAAGGTTGGTGAAGATC TTCGGCAAGATATGTTAGCTTTACAGATGATAAAGATTATGGATAAGATCTGGCTTAAAGAGGGCCTGGA TCTGAGGATGGTGATATTCAAATGCCTGTCGACTGGCCGAGATCAAGGCATGGTGGAGCTAGTTCCTGCC TCAGACACTCTCAGGAAAATCCAAGTGGAATATGGTGTGACAGGATCCTTTAAAGATAAACCACTTGCTG AATGGCTAAGGAAATACAACCCTTCTGAAGAAGAATATGAAAAGGCTTCTGAGAACTTTATCTACTCTTG TGCTGGGTGCTGTGTAGCCACTTATGTTTTAGGCATTTGTGATCGGCACAATGACAATATAATGCTTCGA AGCACAGGACACATGTTCCACATTGACTTTGGAAAGTTTTTGGGCCATGCACAGATGTTTGGTAGCTTCA AAAGGGACCGCGCTCCTTTCGTGCTTACCTCTGACATGGCCTATGTCATTAATGGAGGTGAAAAGCCCAC CATTCGTTTCCAGTTGTTTGTGGACCTCTGCTGTCAAGCCTACAACTTGATAAGAAAGCAAACAAACCTC TTTCTTAACCTTCTCTCACTGATGATTCCTTCAGGATTGCCAGAACTTACAAGTATTCAGGATTTGAAAT ATGTTAGAGATGCACTTCAGCCCCAAACTACAGATGCTGAAGCTACTATTTTCTTTACTAGGCTGATTGA GTCAAGTTTGGGAAGCATTGCCACAAAGTTTAATTTCTTCATTCATAACCTTGCTCAGCTACGTTTTTCT GGCCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCAAAAACATACTCCTTTAGACAAGACGGCC GGATCAAAGAAGTCTCTGTTTTCACATATCATAAGAAATACAACCCAGATAAACACTATATTTATGTGGT TCGAATTCTGAGGGAAGGACACCTTGAACCATCATTTGTATTCCGGACATTTGATGAGTTTCAGGAACTG CACAATAAGCTCAGCATTATTTTCCCACTTTGGAAGTTACCTGGCTTTCCTAATAGGATGGTTCTAGGAA GAACACACATCAAAGATGTAGCAGCCAAGAGGAAAATTGAATTAAACAGTTATTTACAGAGTTTGATGAA TGCATCAACAGATGTAGCAGAGTGTGACCTTGTTTGTACTTTTTTCCACCCCTTACTTCGTGATGAAAAA GCTGAAGGAATAGCTAGGTCTACAGGTACAAGTCCCTTCAGCCCTACTTTGGGCCAAATAGGAGGAGCAG TGAAGTTATCGGTTTCTTACCGAAATGGCACCCTCTTCATCATGGTGATGCACATCAAAGATCTGGTGAC TGAAGATGGGGCTGACCCAAATCCATACGTCAAAACATACCTGCTTCCAGATACCCACAAAACGTCAAAA CGTAAAACCAAAATTTCACGTAAAACTAGGAACCCAACATTCAATGAAATGCTTGTATATAGTGGATACA GCAGAGAAACTCTGAGACAGAGAGAACTTCAACTGAGTGTGCTCAGTGCAGAATCTCTGCGGGAGAATTT CTTCCTGGGTGGAATAACCCTGCCACTGAAAGATTTCAACTTGAGCAAAGAGACAGTTAAGTGGTATCAG CTGACTGCGGCAACATACCTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-MluI
ACCN	NM_001108500
Insert Size	5064 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108500.1, NP_001101970.1
RefSeq Size	8316 bp
RefSeq ORF	5064 bp
Locus ID	361632

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