Abcb4 (NM_012690) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Mdr2; Pgy3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205358 representing NM_012690 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTTGAGGCAGCAAGAAACGGAACAGCGCGGCGCCTGGACGGCGACTTTGAACTAGGCAGCATCA GCAACCAGAGCAGAGAAAAAAAGAAGAAAGTGAATTTAATTGGCCCGTTGACACTGTTCCGATACTCTGA TTGGCAGGATAAATTGTTTATGCTCCTGGGCACCGCCATGGCCATAGCTCACGGATCAGGTCTTCCCCTT ATGATGATAGTCTTTGGAGAAATGACAGATAAGTTTGTAGATAATGCTGGGAACTTTTCCTTGCCAGTGA ATTTTTCATTGTCAATGCTAAATCCAGGAAGAATTCTGGAAGAAGAAATGACTAGATATGCATACTACTA TTCGGGACTAGGTGGTGGAGTTCTTTTGGCTGCCTATATCCAAGTCTCCTTCTGGACTTTGGCAGCTGGC CGACAAATAAGGAAAATCAGGCAAAAATTTTTTCACGCCATCCTTCGACAAGAAATGGGCTGGTTTGATA TCAAGGGCACCACCGAACTCAACACGCGGCTGACAGATGACATCTCCAAAATCAGTGAAGGAATTGGTGA CAAGGTTGGAATGTTCTTTCAAGCAATAGCCACGTTTTTTGCAGGATTCATAGTGGGGTTCATCAGAGGC TGGAAACTCACCCTCGTGATCATGGCCATCACCGCCATCTTGGGGCTCTCTACAGCCGTTTGGGCAAAGA TACTCTCAACATTCAGTGACAAAGAACTAGCTGCCTATGCAAAAGCAGGTGCCGTGGCGGAAGAGGCTCT GGGAGCCATCAGGACCGTGATAGCTTTCGGGGGCCAGAACAAAGAGCTAGAAAGGTATCAGAAGCATTTA GAAAATGCCAAAAAGATTGGAATTAAAAAGGCTATCTCGGCCAACATCTCCATGGGCATTGCCTTTTTGT TAATATATGCATCCTATGCACTGGCCTTCTGGTATGGATCCACTCTGGTTATATCAAAAGAATATACAAT TGGAAATGCCATGACAGTGTTCTTCTCAATCCTCATTGGGGCCTTCAGTGTGGGGCAGGCTGCCCCCTGT ATTGATGCTTTCCCCAATGCTAGAGGAGCAGCCTATGTGATCTTTGACATTATTGATAATAATCCTAAAA TTGACAGTTTTTCAGAGAGAGGACACAAGCCAGACAGCATCAAAGGAAATTTGGAGTTCAGTGACGTTCA CTTTTCCTACCCATCTCGGGCTAATATCAAGATCTTGAAGGGCCTCAACCTGAAGGTGAAGAGCGGGCAG ACGGTAGCCCTGGTTGGCAACAGTGGCTGTGGGAAAAGCACAACTGTCCAGCTGCTGCAGAGGCTCTACG ACCCCACAGAGGGTACGATTAGCATCGATGGGCAGGACATCCGGAACTTTAACGTCAGGTGTCTAAGGGA ATTCATCGGCGTGGTGAGTCAAGAGCCGGTACTGTTCTCTACCACGATTGCTGAAAATATCCGCTATGGC CGTGGGAATGTAACAATGGATGAGATTAAAAAAGCTGTCAAAGAGGCTAATGCCTATGACTTCATCATGA AACTGCCACAGAAATTTGACACCCTGGTTGGTGACAGAGGGGCGCAGCTGAGCGGGGGACAGAAACAGAG GATCGCCATTGCTCGTGCCTTGGTCCGCAACCCCAAGATCCTCCTGCTGGACGAGGCCACGTCAGCCTTG GACACAGAAAGCGAAGCTGAGGTGCAGGCCGCTCTGGATAAGGCCAGAGAAGGCCGGACCACCATCGTGA TAGCTCACCGACTGTCAACTGTCCGGAATGCAGATGTCATCGCTGGGTTTGAGGATGGCGTCATCGTGGA GCAAGGAAGCCACAGTGAGCTGATAAAGAAGGAAGGGATCTACTTCAGACTTGTTAACATGCAGACATCA GGAAGCCAGATCCTGTCAGAAGAATTTGAAGTTGAGCTAAGTGATGAAAAGGCTGCTGGAGGTGTGGCCC CAAATGGCTGGAAAGCACGCATATTTAGGAATTCTACGAAGAAAAGTCTGAAAAGTTCACGGGCGCATCA AAATAGGCTGGATGTGGAAACCAATGAACTTGATGCAAACGTGCCACCAGTGTCTTTTCTGAAGGTCTTA AGACTGAATAAAACAGAGTGGCCCTACTTTGTGGTGGGGACACTCTGTGCCATTGCCAACGGGGCCCTCC AGCCGGCATTCTCCATCATCCTGTCAGAGATGATAGCTATCTTTGGCCCTGGGGATGACACAGTAAAGCA ACAGAAGTGTAACATGTTCTCGCTGGTCTTCTTGGGCCTAGGAGTCCACTCCTTCTTTACTTTCTTCCTT CAGGGTTTCACATTCGGGAAAGCTGGCGAGATCCTCACCACAAGGCTCCGGTCCATGGCCTTCAAAGCAA TGCTAAGACAGGACATGAGCTGGTTTGACGATCATAAAAACAGTACTGGTGCCCTCTCTACAAGACTCGC CACAGACGCTGCGCAGGTCCAAGGAGCCACAGGAACCAGGTTGGCTTTAATTGCACAGAACACAGCCAAC CTTGGAACGGGTATTATTATATCATTTATTTACGGTTGGCAACTGACACTTCTGCTCTTATCAGTTGTTC CATTCATTGCTGTAGCGGGAATTGTTGAAATGAAAATGTTGGCTGGCAACGCCAAGAGAGATAAAAAGGA GATGGAAGCTGCTGGAAAGATTGCAACAGAGGCAATAGAAAATATTCGGACTGTTGTATCCTTGACCCAA GAGAGAAAATTTGAGTCAATGTATGTTGAAAAATTACACGGACCTTACAGGAATTCAGTGCGGAAGGCTC ACATCTACGGCATCACTTTTAGCATCTCACAAGCATTCATGTACTTTTCTTATGCTGGCTGCTTTCGATT TGGTTCTTACCTCATTGTGAATGGACACATGCGCTTCAAGGATGTCATCCTGGTGTTCTCAGCAATCGTG CTTGGTGCAGTGGCTCTAGGACATGCCAGCTCATTTGCTCCAGACTATGCAAAAGCCAAGCTGTCTGCAG CATACTTATTCAGTCTGTTTGAAAGACAACCTCTGATTGACAGCTACAGCAGAGAAGGAATGTGGCCGGA TAAGTTTGAAGGAAGCGTGACATTCAATGAAGTTGTGTTCAATTATCCCACCCGGGCCAATGTGCCAGTG CTTCAGGGGCTGAGCCTCGAGGTGAAGAAGGGGCAGACCCTGGCCCTGGTGGGCAGTAGTGGCTGCGGGA AGAGCACCGTGGTCCAGCTGCTCGAGCGCTTCTACGACCCCATGGCCGGAACAGTGCTCCTCGATGGTCA GGAAGCAAAGAAACTCAATGTCCAGTGGCTCCGAGCTCAACTTGGCATTGTGTCCCAGGAGCCCATCCTG TTTGACTGCAGCATCGCCAAGAACATCGCCTACGGAGACAACAGCCGTGTCGTGTCTCAGGATGAGATTG TGAGGGCGGCCAAGGAGGCCAACATCCACCCCTTCATTGAGACACTGCCCCAAAAGTATGAAACAAGAGT AGGAGACAAGGGGACACAGCTCTCTGGAGGCCAGAAACAGAGGATTGCTATCGCCCGAGCCCTCATCAGA CAGCCTCGGGTCCTACTGCTGGATGAAGCCACGTCGGCTTTGGACACTGAGAGTGAAAAGGTCGTCCAGG AAGCGCTGGACAAAGCCAGGGAAGGCCGCACCTGCATTGTGATCGCGCACCGCCTGTCCACCATCCAGAA CGCAGACTTGATCGTGGTGATCGACAACGGCAAGGTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCC CAGAAAGGCATCTATTTCTCCATGGTCAACATTCAAGCTGGCACACAGAACTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012690
Insert Size	3837 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012690.2, NP_036822.1
RefSeq Size	3910 bp
RefSeq ORF	3837 bp
Locus ID	24891
UniProt ID	Q08201
Gene Summary	energy-dependent efflux pump; may be involved in changes in bile formation in response to diabetes [RGD, Feb 2006]

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