Pc (NM_012744) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Pcx
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205534 representing NM_012744 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAAGTTCCAAACAGTTCGAGGGGGCCTGAGGCTCCTGGGTGTCCGCCGATCCTCCACAGCCCCCG TTGCCTCCCCAAATGTCCGGCGTCTGGAGTACAAGCCCATCAAGAAAGTAATGGTGGCCAACAGAGGTGA GATTGCCATCCGAGTGTTTCGTGCCTGCACAGAGCTGGGTATCCGCACAGTGGCTGTCTACTCGGAGCAG GACACAGGGCAGATGCACCGGCAGAAAGCTGATGAAGCCTACCTTATTGGCCGTGGGCTGGCTCCTGTGC AAGCCTACCTGCACATTCCAGACATCATTAAGGTGGCCAAGGAGAATGGTGTAGATGCTGTGCACCCTGG CTATGGGTTCCTCTCAGAGAGAGCAGACTTTGCCCAGGCCTGCCAAGATGCTGGAGTCCGATTCATTGGT CCAAGCCCAGAGGTGGTCCGCAAGATGGGAGACAAGGTGGAAGCCCGGGCCATTGCCATTGCTGCAGGCG TTCCAGTGGTCCCTGGCACTAATTCCCCCATCAATTCCCTGCATGAGGCACACGAGTTCTCTAACACCTA TGGTTTCCCTATTATCTTCAAGGCTGCCTATGGAGGTGGGGGCCGTGGCATGAGGGTTGTGCATAGCTAC GAGGAGCTGGAAGAGAATTACACCCGGGCCTACTCTGAGGCCTTGGCAGCCTTTGGGAATGGGGCATTGT TTGTGGAGAAATTCATTGAGAAGCCAAGACACATTGAGGTGCAGATCCTAGGGGACCAATATGGGAACAT CTTGCACTTGTATGAGCGGGACTGCTCCATCCAGCGGCGGCACCAGAAGGTCGTCGAGATTGCCCCTGCT ACCCACCTGGACCCCCAACTTCGGTCACGCCTCACCAGTGACTCTGTCAAACTTGCCAAGCAGGTTGGCT ATGAGAATGCAGGCACTGTCGAGTTCCTGGTGGACAAGCATGGCAAGCACTACTTCATCGAGGTCAATTC CCGCCTGCAGGTGGAGCACACGGTCACTGAGGAGATTACAGATGTGGACCTGGTCCATGCTCAGATCCAT GTCTCCGAAGGCCGGAGCCTGCCTGACCTGGGGCTGCGGCAGGAAAACATCCGAATCAATGGTTGTGCCA TTCAGTGTCGGGTCACCACTGAGGACCCTGCACGCAGCTTCCAGCCAGACACTGGCCGCATTGAGGTTTT CCGGAGTGGTGAGGGCATGGGCATCCGCCTGGACAATGCCTCAGCATTCCAGGGAGCTGTCATATCCCCC CACTATGACTCCCTGCTGGTCAAAGTCATTGCCCATGGCAAAGACCACCCTACAGCTGCCACCAAGATGA GCAGAGCCCTGGCGGAGTTCCGTGTCCGAGGTGTAAAGACCAACATCCCCTTCCTGCAGAATGTTCTCAA CAACCAGCAGTTCCTAGCGGGCATTGTGGACACCCAGTTCATCGATGAGAACCCCGAGCTGTTCCAGCTG CGGCCTGCACAGAACCGGGCCCAGAAGTTGCTACATTACCTTGGACACGTCATGGTCAATGGCCCTACCA CTCCAATCCCCGTCAAGGTCAGTCCCAGCCCTGTGGACCCCATTGTTCCTGTGGTGCCCATAGGCCCACC CCCAGCTGGTTTCAGAGACATCCTTCTGCGAGAGGGGCCAGAGGGCTTTGCCAGAGCTGTGCGGAATCAC CAGGGGCTGCTGCTAATGGACACAACCTTCCGGGATGCCCACCAGTCACTACTTGCCACTAGAGTGCGCA CACACGATCTCAAAAAGATTGCACCCTACGTTGCCCACAACTTCAACAACCTCTTCAGCATAGAGAACTG GGGAGGAGCCACATTTGACGTGGCCATGCGCTTCTTGTATGAGTGCCCCTGGCGGCGGCTCCAGGAGCTC CGGGAGCTCATCCCCAACATCCCATTCCAGATGCTACTGAGGGGGGCCAATGCTGTGGGCTACACCAACT ACCCTGACAACGTGGTCTTCAAGTTCTGTGAGGTGGCCAAAGAGAATGGCATGGACGTCTTCCGGATCTT TGACTCCCTTAACTACCTGCCAAACATGCTGCTGGGCATGGAAGCAGCTGGCAGTGCTGGGGGTGTGGTG GAAGCTGCCATCTCCTACACGGGTGACGTGGCTGACCCCAGTCGCACTAAATACTCACTGGAGTACTACA TGGGCTTAGCTGAAGAACTGGTGCGAGCCGGCACTCACATCCTCTGCATTAAGGACATGGCAGGCCTGCT GAAGCCTGCAGCATGCACCATGCTGGTCAGCTCCCTCCGGGACCGGTTCCCCGACCTCCCACTGCACATC CATACCCATGACACATCAGGGTCAGGTGTGGCAGCCATGTTGGCCTGTGCACAAGCTGGGGCTGATGTTG TGGATGTGGCAGTCGACTCTATGTCTGGGATGACCTCACAGCCCAGCATGGGGGCCCTGGTGGCCTGTAC CAAAGGGACTCCTCTGGACACAGAGGTACCCCTGGAGCGTGTGTTTGACTACAGTGAGTATTGGGAAGGG GCTCGGGGGCTGTATGCAGCCTTTGATTGCACGGCTACCATGAAGTCTGGCAACTCAGACGTGTATGAGA ATGAGATCCCAGGGGGCCAGTACACCAACCTACACTTCCAGGCCCACAGCATGGGACTTGGCTCCAAGTT CAAGGAGGTCAAGAAGGCCTATGTGGAGGCTAACCAGATGCTGGGGGACCTCATCAAGGTGACACCATCC TCCAAGATTGTGGGGGATCTGGCCCAGTTCATGGTGCAGAACGGGTTGAGCCGGGCAGAGGCAGAAGCTC AGGCAGAAGAGCTGTCCTTCCCCCGCTCTGTGGTGGAGTTCCTCCAGGGCTACATTGGCATTCCCCATGG GGGTTTCCCTGAACCCTTCCGTTCTAAGGTGCTAAAGGACCTGCCAAGAATAGAAGGGAGGCCTGGAGCC TCCCTCCCTCCCTTGAACCTGAAGGAGCTGGAGAAGGACCTGATTGATAGGCATGGAGAGGAGGTGACCC CAGAGGACGTTCTCTCTGCAGCCATGTACCCTGATGTCTTTGCTCAGTTCAAAGACTTCACGGCTACCTT TGGCCCCCTGGATAGCCTGAATACTCGTCTCTTTCTTCAAGGACCCAAAATTGCAGAGGAGTTTGAGGTT GAGCTGGAACGGGGCAAGACCTTGCACATCAAAGCCCTGGCTGTAAGCGACCTGAACCGTGCTGGCCAGA GGCAGGTGTTCTTTGAACTCAATGGGCAGCTTCGATCCATTCTGGTTAAAGACACCCAGGCCATGAAGGA GATGCACTTCCATCCCAAGGCCTTGAAGGATGTGAAGGGCCAAATTGGGGCCCCTATGCCTGGGAAGGTC ATAGACGTCAAGGTGGCAGCAGGAGCCAAGGTGGTTAAGGGCCAGCCCCTCTGTGTGCTCAGCGCCATGA AGATGGAGACTGTGGTGACTTCGCCCATGGAGGGCACTATCCGAAAGGTTCACGTGACCAAGGACATGAC TCTGGAAGGCGATGACCTCATCCTAGAGATTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012744
Insert Size	3537 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012744.2, NP_036876.2
RefSeq Size	4024 bp
RefSeq ORF	3537 bp
Locus ID	25104
UniProt ID	P52873
Gene Summary	biotin-containing enzyme that catalyses the carboxylation of pyruvate to form oxaloacetate [RGD, Feb 2006]

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